Influence of Adult Mesenchymal Stem Cells on In Vitro Vascular Formation

Adult Mesenchymal stem cells-derived exosomes carry several biologically active molecules that play prominent roles in controlling disease manifestations. The content of these exosomes, their functions, and effect on the immune cells may differ depending on their tissue sources. Therefore, in this study, we purified the exosomes from three different sources and, using the RNA-Seq approach, highly abundant microRNAs were identified and compared between exosomes and parental cells. The effects of exosomes on different immune cells were studied in vitro by incubating exosomes with PBMC and neutrophils and assessing their functions. The expression levels of several miRNAs varied within the different MSCs and exosomes. Additionally, the expression profile of most of the miRNAs was not similar to that of their respective sources. Exosomes isolated from different sources had different abilities to induce the process of neurogenesis and angiogenesis. Moreover, these exosomes demonstrated their varying effect on PBMC proliferation, neutrophil survival, and NET formation, highlighting their versatility and broad interaction with immune cells. The knowledge gained from this study will improve our understanding of the miRNA landscape of exosomes from hMSCs and provide a resource for further improving our understanding of exosome cargo and their interaction with immune cells.

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Effects of steroids on the morphology and proliferation of canine and equine mesenchymal stem cells of adipose origin — in vitro research

Acta Veterinaria Hungarica ◽

10.1556/avet.2014.001 ◽

2014 ◽

Vol 62 (3) ◽

pp. 317-333 ◽

Cited By ~ 5

Author(s):

Krzysztof Marycz ◽

Agnieszka Śmieszek ◽

Jakub Grzesiak ◽

Jakub Nicpoń

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Medical Practice ◽

Proliferation Rate ◽

Cellular Activity ◽

Simultaneous Application ◽

Steroid Injections ◽

Adult Mesenchymal Stem Cells ◽

Steroid Drugs

Disorders of the locomotive system, especially those occurring due to degenerative changes of the joints, are serious problems in daily veterinary medical practice. Steroid injections are the main way of treating these disorders. However, this approach brings usually only temporary effects of pain relief, and may cause many side effects. Alternative therapies focus on regeneration of damaged tissue using adult mesenchymal stem cells (MSCs). Since 2002, the great plasticity and immunomodulatory properties of MSCs isolated from adipose tissue (AdMSCs) have been used successfully in the treatment of degenerative joint diseases (DJD) of both dogs and horses. Possible simultaneous application of steroid therapy and stem cell transplantation could improve the commonly used clinical procedure. In this paper, the influence of the two steroid drugs (betamethasone and methylprednisolone) on AdMSCs was evaluated on the basis of morphology and proliferation rate. Both steroids positively influenced the viability and proliferation state of cells in a concentration of 0.01 mg/ml and 0.1 mg/ml, respectively. However, the concentration of 1 mg/ml had a cytotoxic effect. Moreover, the lower dosage of steroid drugs used in the experiment did not affect the morphology of cells and significantly increased cellular activity. In conclusion, our data demonstrate the stimulating effect of steroid drugs on cell morphology, proliferation rate and cytophysiological activity. These findings may influence the use of stem cells and steroids in applied regenerative veterinary medical practice in the future.

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Isolation, Culture, In-Vitro Differentiation and Characterization of Canine Adult Mesenchymal Stem Cells

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-014-0309-8 ◽

2014 ◽

Vol 84 (4) ◽

pp. 875-884 ◽

Cited By ~ 1

Author(s):

Sarabjot Singh ◽

Dipak Deka ◽

Raghunath Mulinti ◽

Naresh Kumar Sood ◽

Ravi Kant Agrawal ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

In Vitro Differentiation ◽

Culture In Vitro ◽

Adult Mesenchymal Stem Cells

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RAT BONE MARROW MESENCHYMAL STEM CELLS ISOLATION, CULTIVATION AND DIFFERENTIATION

Cluj Veterinary Journal ◽

10.52331/cvj.v15i1.6 ◽

2009 ◽

Vol 15 (1) ◽

pp. 30-34

Author(s):

Emoke PALL ◽

Ioan GROZA ◽

Olga SORITAU ◽

Ciprian TOMULEASA ◽

Mihai CENARIU ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Bone Marrow Aspiration ◽

Homogeneous Population ◽

Adult Rat ◽

Adult Mesenchymal Stem Cells ◽

Marrow Mesenchymal Stem Cells ◽

Rat Bone

Bone marrow stromal cells (MSCs) represent a heterogeneous population derived from the non–blood-forming fraction of bone marrow that regulates hematopoietic cell development. In vitro, adult mesenchymal stem cells resident in this bone marrow fraction differentiate into bone, cartilage, and fat. Because MSCs can be easily obtained using a simple bone marrow aspiration and show extensive capacity for expansion in vitro, these cells have been considered as candidates for cell therapy. The aim of this study was to purify rat MSCs from adult bone marrow and to functionally characterise their abilities to differentiate along diverse lineages. Our data demonstrate that we successfully isolated, culture-expanded and differentiated a relatively homogeneous population of MPCs from adult rat bone marrow.

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Faculty Opinions recommendation of Fibroblast growth factor receptors in in vitro and in vivo chondrogenesis: relating tissue engineering using adult mesenchymal stem cells to embryonic development.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.3374963.3071054 ◽

2010 ◽

Author(s):

Mauro Alini ◽

Martin Stoddart

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Fibroblast Growth ◽

Fibroblast Growth Factor Receptors ◽

Adult Mesenchymal Stem Cells

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Human Glioblastoma-Derived Mesenchymal Stem Cell to Pericytes Transition and Angiogenic Capacity in Glioblastoma Microenvironment

Cellular Physiology and Biochemistry ◽

10.1159/000488429 ◽

2018 ◽

Vol 46 (1) ◽

pp. 279-290 ◽

Cited By ~ 17

Author(s):

Dongye Yi ◽

Wei Xiang ◽

Qing Zhang ◽

Yongcun Cen ◽

Qing Su ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Conditioned Medium ◽

Blue Staining ◽

Alizarin Red ◽

Differentiation Potential ◽

And Migration ◽

Proliferation And Migration ◽

Vascular Formation

Background/Aims: Tumor vascular formation and maintenance are crucial events in glioblastoma development. Mesenchymal stem cells (MSCs) have been shown to differentiate into pericytes and contribute to neovascularization in the glioma microenvironment. Moreover, glioblastoma-derived mesenchymal stem cells (gb-MSCs), which consist of CD90-MSCs and CD90+MSCs, are a subpopulation of MSCs that are more active in glioma vascularization. However, the functions of gb-MSCs and the microRNA (miRNA) modifications in the glioblastoma microenvironment have not yet been fully elucidated. Here, we focus on the pericyte differentiation potential of gb-MSCs and miRNA modifications in gb-MSCs during new vascular formation and glioblastoma growth. Methods: In vitro, surface markers of gb-MSCs were detected by flow cytometry; the differentiation potential was evaluated by Oil Red O staining, Alizarin Red staining and Alcian blue staining; the proliferation and migration of gb-MSCs in different conditioned media were analyzed by the cck8 test and wound-healing assay, respectively; gb-MSC to pericyte transition was detected by immunofluorescence staining and western blot assay; angiogenetic capacity was analyzed by tube formation assay; and levels of cytokines in different supernatant were determined by ELISA. Additionally, RNA was isolated from gb-MSCs, and miRNA modifications were analyzed using the RAffymetrix miRNA microarray Results: We showed that glioblastoma-conditioned medium increased gb-MSC proliferation and migration and was capable of inducing gb-MSC differentiation into pericytes. Glioblastoma secreted angiogenic factors and gb-MSCs incubated in malignant glioblastoma-conditioned medium formed more tube-like structures, and these cells also adhered to tube-like vessels formed by human umbilical vein endothelial cells (HUVECs) on Matrigel to maintain tumor vascular structure in vitro. miRNA expression were also modified in gb-MSCs cultured in malignant glioblastoma-conditioned medium in vitro. Conclusion: These results provide new insight into the functional effects of a subpopulation of MSCs in glioblastoma and may help in the development of novel therapies for solid tumors.

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