Indonesian propolis suppressed the expression of COX-2 in inflamed rat dental pulp in direct capping treatment

Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13 μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children’s teeth.

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Eckols reduce dental pulp inflammation through the ERK1/2 pathway independent of COX-2 inhibition

Oral Diseases ◽

10.1111/odi.12266 ◽

2014 ◽

pp. n/a-n/a ◽

Cited By ~ 3

Author(s):

U Paudel ◽

Y-H Lee ◽

T-H Kwon ◽

N-H Park ◽

B-S Yun ◽

...

Keyword(s):

Dental Pulp ◽

Pulp Inflammation ◽

Cox 2

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Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells

Inflammation Research ◽

10.1007/s00011-005-0013-5 ◽

2006 ◽

Vol 55 (2) ◽

pp. 78-84 ◽

Cited By ~ 38

Author(s):

T. Kojima ◽

M. Yamaguchi ◽

K. Kasai

Keyword(s):

Substance P ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells ◽

Cox 2

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Preconditioning with Melatonin Enhances Osteogenic Differentiation of Dental Pulp Mesenchymal Stem Cells by Regulating MAPK Pathways and Promotes the Efficiency of Bone Regeneration in Calvarial Bone Defects

10.21203/rs.3.rs-936574/v1 ◽

2021 ◽

Author(s):

Ya-Hui Chan ◽

Kuo-Ning Ho ◽

Yu-Chieh Lee ◽

Meng-Jung Chou ◽

Wei-Zhen Lew ◽

...

Keyword(s):

Stem Cells ◽

Bone Regeneration ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Bone Defects ◽

Calvarial Bone ◽

Alp Activity ◽

Cox 2

Abstract Background:Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival of engrafted stem cells remain challenging. Melatonin can regulate MSC biology. However, its function in the osteogenic differentiation of dental pulp-derived MSCs (DPSCs) remains unclear. We investigated the effects and mechanisms of melatonin preconditioning on the osteogenic differentiation and bone regeneration capacities of DPSCs.Methods:The biological effects and signaling mechanisms of melatonin with different concentrations on DPSCs were evaluated using a proliferation assay, the quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, a real-time polymerase chain reaction, and a western blot in vitro cell culture model. The in vivo bone regeneration capacities were assessed among empty control, MBCP, MBCP+DPSCs, and MBCP+DPSCs+melatonin preconditioning in four-created calvarial bone defects by using micro–computed tomographic, histological, histomorphometric, and immunofluorescence analyses after 4 and 8 weeks of healing.Results:In vitro experiments revealed that melatonin (1, 10, and 100 μM) significantly and concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity and extracellular calcium deposition, and osteogenic gene expression of DPSCs (p < 0.05). Melatonin activated the phosphorylation of RUNX-2 and OCN and inhibited COX-2/NF-κB phosphorylation. Furthermore, the phosphorylation of mitogen-activated protein kinase (MAPK) P38/ERK signaling was significantly increased in DPSCs treated with 100 μM melatonin, and their inhibitors significantly decreased osteogenic differentiation. In vivo experiments demonstrated that bone defects implanted with MBCP bone-grafting materials and melatonin-preconditioned melatonin exhibited significantly greater bone volume fraction, trabecular bone structural modeling, new bone formation, and osteogenesis-related protein expression than the other three groups at 4 and 8 weeks postoperatively (p < 0.05).Conclusions:These results suggest that melatonin promotes the proliferation and osteogenic differentiation of DPSCs by regulating COX-2/NF-κB and p38/ERK MAPK signaling pathways. Preconditioning DPSCs with melatonin before transplantation can efficiently enhance MSC function and regenerative capacities.

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POTENSI KOMBINASI EKSTRAK AKAR SIDAGURI (Sida rhombifolia L.) DAN GETAH JARAK (Jatropha curcas L.) SEBAGAI BAHAN DEVITALISASI

ODONTO Dental Journal ◽

10.30659/odj.6.0.14-20 ◽

2019 ◽

Vol 6 ◽

pp. 14

Author(s):

Maria Tanumihadja ◽

Indrya Kirana Mattulada ◽

Nurhayaty Natsir ◽

Lukman Muslimin

Keyword(s):

Blood Vessels ◽

Jatropha Curcas ◽

Dental Pulp ◽

Histopathological Examination ◽

Jatropha Curcas L ◽

Cox 2 ◽

Sida Rhombifolia ◽

Alternative Drugs

Background: Arsenic paste was used to devitalize dental pulp in some problems before surgical, but it has been proven to have many hazardous effects. So, other alternative drugs are needed that are equally effective but safer. Jatropha (Jatropha curcas L.) can cause lysis blood vessels in the pulp, whereas the sidaguri (Sidarhombifolia L.) are effective in relieving inflammation. The purpose of this study was to determine the devitalization effect of jatropha and sidaguri on rabbit animal model.Method: Sidaguri roots were extracted using reflux method while jatropha sap was dried using lyophilization method. For easy application, the extract made into a paste (1; 1) and inserted into the cavity. After 7 days of exposure, the teeth were removed, calcified and followed to histopathological staining and COX-2 expression (IHC).Result: Histopathological examination showed that the paste was able to cause necrosis of pulp nerve. Higher dose of the drug increased the necrosis area, but the paste is not able to reduce COX-2 expression.Conclusion: It can be concluded that the paste has a potential to developed as devitalization agent although it is necessary to study to the reduce the inflammation process.

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Synergy of TLR2 and H1R on Cox-2 Activation in Pulpal Cells

Journal of Dental Research ◽

10.1177/0022034509354720 ◽

2009 ◽

Vol 89 (2) ◽

pp. 180-185 ◽

Cited By ~ 7

Author(s):

C. Park ◽

S.Y. Lee ◽

H.J. Kim ◽

K. Park ◽

J.S. Kim ◽

...

Keyword(s):

Dental Pulp ◽

Histamine Receptor ◽

Cell Type ◽

Microbial Infection ◽

Synergistic Activation ◽

Major Cell Type ◽

Cox 2 ◽

Micro Organisms ◽

Stimulation Of

Although pulp fibroblasts are a major cell type in dental pulp, their roles in microbial recognition and pulpal inflammation are not well-understood. Considering the pivotal role of Toll-like receptors (TLRs) in the recognition of micro-organisms, we hypothesized that TLRs on pulp fibroblasts may induce inflammatory signals in dental pulp. In human pulp fibroblasts, TLR2, 3, 4, and 5 were constitutively expressed. Stimulation of TLR2 and 3 induced the expression of pro-inflammatory genes such as CXCL10, CCL5, and/or Cox-2 in pulp fibroblasts. Interestingly, histamine synergistically activated TLR2-mediated Cox-2 expression and PGE2 production. The synergistic effect of histamine is mediated by histamine receptor-1 (H1R). Studies on the intra-cellular signaling pathways revealed that p38 activation is required for the synergistic activation of Cox-2 by TLR2 and histamine. Analysis of these data suggests that TLR2 on pulp fibroblasts, in concert with H1R, can induce an inflammatory response during microbial infection in dental pulp.

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