scholarly journals Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (Eugenol) on Inflammatory and Apoptosis Processes in Dental Pulp Fibroblasts

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Andrea Martínez-Herrera ◽  
Amaury Pozos-Guillén ◽  
Socorro Ruiz-Rodríguez ◽  
Arturo Garrocho-Rangel ◽  
Antonio Vértiz-Hernández ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Primary Teeth ◽  
Third Molars ◽  
Pulp Therapy ◽  
Inflammatory Agent ◽  
Zinc Oxide Powder ◽  
Pulp Inflammation ◽  
Apoptotic Genes ◽  
Negative Controls ◽  
Direct Capping

Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13 μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children’s teeth.

scholarly journals Pulpal Diagnosis of Primary Teeth: Guidelines for Clinical Practice

2013 ◽  
Vol 2 (2) ◽  
pp. 65-68 ◽  
Author(s):  
Golam Mohammad ◽  
Farjana Jerin ◽  
Suraya Jebin
Keyword(s):  
Decision Making ◽  
Dental Pulp ◽  
Primary Teeth ◽  
Pediatric Dentistry ◽  
Evidence Based ◽  
Good Decision ◽  
Pulp Therapy ◽  
Reliable Assessment ◽  
Therapy Technique ◽  
History Of

Diagnosis of pulp status is an important clinical step to achieve success in pulp therapy technique or endodontic treatment in children. In pediatric dentistry, history of symptoms given by a child may not be reliable. Assessment of dental pulp status plays an important role. It is hoped that these guidelines will facilitate pulpal diagnosis, good decision-making and evidence-based practice for pediatric patients.DOI: http://doi.dx.org/10.3329/bjdre.v2i2.16248 Bangladesh Journal of Dental Research & Education Vol.2(2) 2012: 66-68

Dental pulp therapy for primary teeth in children undergoing cancer therapy

2014 ◽  
Vol 61 (12) ◽  
pp. 2297-2301 ◽  
Author(s):  
Elinor Halperson ◽  
Dinna Moss ◽  
Nili Tickotsky ◽  
Michael Weintraub ◽  
Moti Moskovitz
Keyword(s):  
Cancer Therapy ◽  
Dental Pulp ◽  
Primary Teeth ◽  
Pulp Therapy

scholarly journals Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jie Chen ◽  
Huaxing Xu ◽  
Kun Xia ◽  
Shuhua Cheng ◽  
Qi Zhang
Keyword(s):  
Dental Pulp ◽  
Tissue Repair ◽  
Pulp Tissue ◽  
Tissue Repair And Regeneration ◽  
Injury Model ◽  
Tnf Α ◽  
Pulp Inflammation ◽  
Inflammation Resolution ◽  
Pulp Repair ◽  
Dentin Regeneration

Abstract Background Unresolved inflammation and tissue destruction are considered to underlie the failure of dental pulp repair. As key mediators of the injury response, dental pulp stem cells (DPSCs) play a critical role in pulp tissue repair and regeneration. Resolvin E1 (RvE1), a major dietary omega-3 polyunsaturated fatty-acid metabolite, is effective in resolving inflammation and activating wound healing. However, whether RvE1 facilitates injured pulp-tissue repair and regeneration through timely resolution of inflammation and rapid mobilization of DPSCs is unknown. Therefore, we established a pulp injury model and investigated the effects of RvE1 on DPSC-mediated inflammation resolution and injured pulp repair. Methods A pulp injury model was established using 8-week-old Sprague-Dawley rats. Animals were sacrificed on days 1, 3, 7, 14, 21, and 28 after pulp capping with a collagen sponge immersed in PBS with RvE1 or PBS. Hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry, and immunohistofluorescence were used to evaluate the prohealing properties of RvE1. hDPSCs were incubated with lipopolysaccharide (LPS) to induce an inflammatory response, and the expression of inflammatory factors after RvE1 application was measured. Effects of RvE1 on hDPSC proliferation, chemotaxis, and odontogenic differentiation were evaluated by CCK-8 assay, transwell assay, alkaline phosphatase (ALP) staining, alizarin red staining, and quantitative PCR, and possible signaling pathways were explored using western blotting. Results In vivo, RvE1 reduced the necrosis rate of damaged pulp and preserved more vital pulps, and promoted injured pulp repair and reparative dentin formation. Further, it enhanced dentin matrix protein 1 and dentin sialoprotein expression and accelerated pulp inflammation resolution by suppressing TNF-α and IL-1β expression. RvE1 enhanced the recruitment of CD146+ and CD105+ DPSCs to the damaged molar pulp mesenchyme. Isolated primary cells exhibited the mesenchymal stem cell immunophenotype and differentiation. RvE1 promoted hDPSC proliferation and chemotaxis. RvE1 significantly attenuated pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) release and enhanced ALP activity, nodule mineralization, and especially, expression of the odontogenesis-related genes DMP1, DSPP, and BSP in LPS-stimulated DPSCs. RvE1 regulated AKT, ERK, and rS6 phosphorylation in LPS-stimulated DPSCs. Conclusions RvE1 promotes pulp inflammation resolution and dentin regeneration and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. This response is, at least partially, dependent on AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 has promising application potential in regenerative endodontics.

scholarly journals Reactionary Versus Reparative Dentine in Deep Caries

2019 ◽  
Vol 25 (1) ◽  
pp. 15-21
Author(s):  
Iliescu Alexandru-Andrei ◽  
Gheorghiu Irina-Maria ◽  
Tănase Mihaela ◽  
Iliescu Andrei ◽  
Mitran Loredana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Complex Response ◽  
Pulp Inflammation ◽  
Proliferation And Differentiation ◽  
Molecular Signals

Abstract The dentine-pulp complex response in deep caries is histological characterized by tertiary formation and mild chronic pulp inflammation. The quiescent primary odontoblasts are reactivated, laying down reactionary tertiary dentine. In more severe carious damage the primary odontoblasts die and reparative tertiary dentine is secreted by odontoblast-like cells, which are differentiated in adult teeth mainly from dental pulp stem cells DPSC. Though associated with reversible pulpitis DPSC still preserve in deep caries the capability of migration, proliferation and differentiation. Some common mechanisms of molecular signals involved in tertiary dentine formation might also explain the balance between inflammation and regeneration of dentine-pulp complex.

scholarly journals Multiple Pulp Stones in Primary and Developing Permanent Dentition: A Report of 4 Cases

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Mohita Marwaha ◽  
Radhika Chopra ◽  
Payal Chaudhuri ◽  
Atul Gupta ◽  
Jayna Sachdev
Keyword(s):  
Liver Function ◽  
Liver Function Test ◽  
Kidney Function ◽  
Dental Pulp ◽  
Metabolic Disorders ◽  
Primary Teeth ◽  
Permanent Teeth ◽  
Metabolic Evaluation ◽  
Function Test ◽  
Pulp Stones

Pulp stones are foci of calcification or discrete calcifications in the dental pulp. They are frequently found on bitewing and periapical radiographs, but their occurrence in entire dentition is unusual. We are reporting four cases in which the occurrence of pulp stones ranged from their presence in just primary teeth (Cases 1 and 2) to involvement of young permanent teeth also (Case 3) and even unerupted permanent teeth (Case 4). In all the cases, dental, medical, and family histories as well as the findings from the clinical examination of the patient were not contributory. Histopathological report revealed true denticle. Metabolic evaluation of patients through liver function test, kidney function test, and blood investigation did not show any metabolic disorders. Patients were also evaluated for any systemic, syndromic, or genetic involvement, but this was also noncontributing. Therefore, it is suggested that these unusual cases may be of idiopathic origin.

scholarly journals Micro‐XRD and nanoindentation investigation of bioceramics for dental pulp therapy

2019 ◽  
Vol 2 (1) ◽  
pp. e10027
Author(s):  
Satish Alapati ◽  
Masahiro Iijima ◽  
William A. Brantley ◽  
Shuichi Ito ◽  
Takeshi Muguruma ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Pulp Therapy

scholarly journals Melatonin as an Agent for Direct Pulp-Capping Treatment

2020 ◽  
Vol 17 (3) ◽  
pp. 1043 ◽  
Author(s):  
Julia Guerrero-Gironés ◽  
Antonia Alcaina-Lorente ◽  
Clara Ortiz-Ruiz ◽  
Eduardo Ortiz-Ruiz ◽  
María P. Pecci-Lloret ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dental Pulp ◽  
Bone Growth ◽  
Study Groups ◽  
Histological Evaluation ◽  
Sprague Dawley ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Pulp Inflammation

Melatonin plays an essential role in the regulation of bone growth. The actions that melatonin exerts on odontoblasts may be similar to its action on osteoblasts. This research aimed to evaluate the pulp response to melatonin used for direct pulp capping to evaluate the antioxidant effect of melatonin administered orally and its influence on dental pulp. Direct pulp capping was performed on the upper molars of Sprague Dawley rats using melatonin or Mineral Trioxide Aggregate (MTA). The study groups were: MTA; Melatonin; MTA + Melatonin administered orally; and Melatonin + Melatonin administered orally. In the latter two groups, the animals drank water dosed with melatonin ad libitum (10 mg/100 mL). After 30 days, the animals were sacrificed, and 5 ml of blood, the kidneys, and the liver were extracted in order to evaluate oxidative stress using thiobarbituric acid reactive substances testing (TBARS). Fragments of the maxilla containing the study molars were prepared for histological evaluation. The degree of pulp inflammation and pulp necrosis, the presence of reparative dentin and dentin bridging the pulp chamber, the presence and regularity of the odontoblastic layer, and the presence of pulp fibrosis were evaluated. No significant differences were found between the four study groups for any of the studied histological variables. The oral administration of melatonin did not modify the local effects of MTA or melatonin on dental pulp, or reduce basal-level oxidative stress. The effect of melatonin on pulp is similar to that of MTA and may be used as an agent for direct pulp capping.

scholarly journals Expression of early angiogenesis indicators in mature versus immature teeth

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Luis F. Lopez-Moncayo ◽  
Hernan Dario Muñoz-Alvear ◽  
Francisco Hernandez-Acosta ◽  
Melissa Pantoja-Mora ◽  
...  
Keyword(s):  
Root Development ◽  
Dental Pulp ◽  
Cell Metabolism ◽  
Elisa Test ◽  
Physiological Process ◽  
Oxygen Balance ◽  
Third Molars ◽  
Angiogenic Activity ◽  
Immature Teeth ◽  
Development Group

Abstract Background Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF-1α, as well as vascular destabilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF-1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development. Methods Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at − 80 °C, defrosted in an ice bath and re-frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. Student t and Mann–Whitney U tests were performed to determine statistically significant differences between groups. Results In the complete root development, HIF-1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group. Conclusions The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.

Sign in / Sign up

Export Citation Format

Share Document