Microfabricated, Wireless, Magnetoelastic Micro-Particles for the Detection of Bacillus Anthracis Spores

2006 ◽  
Vol 951 ◽  
Author(s):  
Jiehui Wan ◽  
Michael L. Johnson ◽  
Huihua Shu ◽  
Valery A. Petrenko ◽  
Bryan A. Chin
Keyword(s):  
Resonance Frequency ◽  
Bacillus Anthracis ◽  
Biomolecular Recognition ◽  
Sensor Surface ◽  
Recognition Element ◽  
Specific Phage ◽  
Micro Particles ◽  
Bacillus Anthracis Spores ◽  
Molecular Recognition Element ◽  
Magnetoelastic Resonance

ABSTRACTMagnetoelastic resonance biosensors were fabricated by immobilizing a bio-molecular recognition element onto the surface of Fe79B21 magnetoelastic particles (MEP). These sensors can be measured wirelessly and remotely for both in-air and in-liquid bacteria detection. Filamentous bacterio-phage that was selected specifically for the detection of Bacillus anthracis spores was employed as the biomolecular recognition element and immobilized onto the MEPs' surfaces. Attachment of the spores to the sensor surface due to specific phage-spore binding results in a shift in the resonance frequency of the biosensor. Insitu measurement of the resonance frequency of biosensors of 5×100×500 microns were used to determine the sensor response as a function of spore concentrations of 103 to 108 cfu/ml. Specificity of the sensor was evaluated by conducting tests using a mixture of Bacillus anthracis Sterne strain, Bacillus cereus and Bacillus megaterium spores.

Detection of Bacillus Anthracis Spores Using Magnetostrictive Microcantilever-based Biosensor

2006 ◽  
Vol 951 ◽  
Author(s):  
Liling Fu ◽  
Suiqiong Li ◽  
Kewei Zhang ◽  
Z.-Y. Cheng
Keyword(s):  
Detection Limit ◽  
Acoustic Wave ◽  
Resonance Frequency ◽  
Bacillus Anthracis ◽  
High Performance ◽  
Filamentous Phage ◽  
Q Value ◽  
High Q ◽  
Bacillus Anthracis Spores ◽  
Higher Sensitivity

ABSTRACTRecently, the magnetostrictive microcantilever (MSMC) as a high performance biosensor platform was introduced. The MSMC is a wireless acoustic wave (AW) sensor and exhibits a high Q value. More importantly, the MSMC works well in liquid. In this paper, the detection of Bacillus anthracis spores using MSMCs with filamentous phage as the bioprobe is reported. The phased-coated MSMC biosensors were exposed to cultures containing target spores with increasing concentrations ranging from 5 × 104 to 5 × 108 spores/mL. By monitoring the shift in the resonance frequency of the MSMCs, the spores were detected in a real-time manner and a detection limit of 105 spores/mL was obtained for the MSMCs used in this research. Higher sensitivity is expected for the MSMCs with smaller size.

Dendritic Cells Endocytose Bacillus Anthracis Spores: Implications for Anthrax Pathogenesis

2005 ◽  
Author(s):  
Katherine C. Brittingham ◽  
Gordon Ruthel ◽  
Rekha G. Panchal ◽  
Claudette L. Fuller ◽  
Wilson J. Ribot
Keyword(s):  
Dendritic Cells ◽  
Bacillus Anthracis ◽  
Bacillus Anthracis Spores

scholarly journals Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(vinyl chloride) Coated Piezoelectric Crystal Immunosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ali Oztuna ◽  
Hasan Nazir ◽  
Mehmet Baysallar
Keyword(s):  
Bacillus Anthracis ◽  
Vinyl Chloride ◽  
Quartz Crystal ◽  
Adhesive Layer ◽  
Antibody Immobilization ◽  
Potential Threat ◽  
Amine Functionalized ◽  
Poly Vinyl Chloride ◽  
Bacillus Anthracis Spores ◽  
Flow Through

Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.

scholarly journals Murine splenocytes produce inflammatory cytokines in a MyD88-dependent response to Bacillus anthracis spores

2007 ◽  
Vol 9 (2) ◽  
pp. 502-513 ◽  
Author(s):  
Ian J. Glomski ◽  
Jörg H. Fritz ◽  
Selina J. Keppler ◽  
Viviane Balloy ◽  
Michel Chignard ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Inflammatory Cytokines ◽  
Murine Splenocytes ◽  
Bacillus Anthracis Spores

scholarly journals Multigeneration Cross-Contamination of Mail with Bacillus anthracis Spores

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0152225 ◽  
Author(s):  
Jason Edmonds ◽  
H. D. Alan Lindquist ◽  
Jonathan Sabol ◽  
Kenneth Martinez ◽  
Sean Shadomy ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Cross Contamination ◽  
Bacillus Anthracis Spores

Phage as a molecular recognition element in biosensors immobilized by physical adsorption

2007 ◽  
Vol 22 (6) ◽  
pp. 986-992 ◽  
Author(s):  
Viswaprakash Nanduri ◽  
Iryna B. Sorokulova ◽  
Alexandre M. Samoylov ◽  
Aleksandr L. Simonian ◽  
Valery A. Petrenko ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Physical Adsorption ◽  
Recognition Element ◽  
Molecular Recognition Element

RAZOR™ EX Anthrax Air Detection System for Detection of Bacillus anthracis Spores from Aerosol Collection Samples: Collaborative Study

2013 ◽  
Vol 96 (2) ◽  
pp. 392-398 ◽  
Author(s):  
Ted Hadfield ◽  
Valorie Ryan ◽  
Usha K Spaulding ◽  
Kristine M Clemens ◽  
Irene M Ota ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Bacillus Anthracis ◽  
Detection System ◽  
Collaborative Study ◽  
Probability Of Detection ◽  
Data Sets ◽  
Method Performance ◽  
Bacillus Anthracis Spores ◽  
Phosphate Buffered Saline ◽  
Positive Results

Abstract The RAZOR™ EX Anthrax Air Detection System was validated in a collaborative study for the detection of Bacillus anthracis in aerosol collection buffer. Phosphate-buffered saline was charged with 1 mg/mL standardized dust to simulate an authentic aerosol collection sample. The dust-charged buffer was spiked with either B. anthracis Ames at 2000 spores/mL or Bacillus cereus at 20 000 spores/mL. Twelve collaborators participated in the study, with four collaborators at each of three sites. Each collaborator tested 12 replicates of B. anthracis in dust-charged buffer and 12 replicates of B. cereus in dust-charged buffer. All samples sets were randomized and blind-coded. All collaborators produced valid data sets (no collaborators displayed systematic errors) and there was only one invalid data point. After unblinding, the analysis revealed a cross-collaborator probability of detection (CPOD) of 1.00 (144 positive results from 144 replicates, 95% confidence interval 0.975–1.00) for the B. anthracis samples and a CPOD of 0.00 (0 positive results from 143 replicates, 95% confidence interval 0.00–0.0262) for the B. cereus samples. These data meet the requirements of AOAC Standard Method Performance Requirement 2010.003, developed by the Stakeholder Panel on Agent Detection Assays.

Phage coated magnetoelastic micro-biosensors for real-time detection of Bacillus anthracis spores☆

2009 ◽  
Vol 137 (2) ◽  
pp. 501-506 ◽  
Author(s):  
Wen Shen ◽  
Ramji S. Lakshmanan ◽  
Leslie C. Mathison ◽  
Valery A. Petrenko ◽  
Bryan A. Chin
Keyword(s):  
Bacillus Anthracis ◽  
Real Time ◽  
Bacillus Anthracis Spores ◽  
Real Time Detection

scholarly journals Direct Colorimetric Detection of Virus by a Polymerized Bilayer Assembly

1993 ◽  
Vol 330 ◽  
Author(s):  
Deborah H. Charych ◽  
Jon O. Nagy ◽  
Wayne Spevak ◽  
Joel Ager ◽  
Mark D. Bednarski
Keyword(s):  
Color Change ◽  
Colorimetric Detection ◽  
Self Assembled Monolayer ◽  
Visible Absorption ◽  
Polymer Backbone ◽  
Color Transition ◽  
Recognition Element ◽  
Ligand Interactions ◽  
Molecular Recognition Element ◽  
Bilayer Assembly

ABSTRACTScreening and detecting virus by receptor-ligand interactions presents an important challenge in medical and environmental diagnostics, and in drug development. We have developed a direct colorimetric detection method based on a polymeric bilayer assembly. The bilayer is composed of a self-assembled monolayer of octadecyl siloxane and a Langmuir-Blodgett layer of polydiacetylene. The polydiacetylene layer is functionalized with receptor-specific ligands such as analogs of sialic acid. The ligand serves as a molecular recognition element, while the conjugated polymer backbone signals binding at the surface by a chromatic transition. The color transition is readily visible to the naked eye as a blue to red color change and can be quantified by visible absorption spectroscopy. The color transition can be inhibited by the presence of soluble inhibitors. Raman spectroscopic analysis shows that the color transition may arise from binding induced strain on the material resulting in bond elongation and conjugation length reduction.

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