Detection of Bacillus Anthracis Spores Using Magnetostrictive Microcantilever-based Biosensor

2006 ◽  
Vol 951 ◽  
Author(s):  
Liling Fu ◽  
Suiqiong Li ◽  
Kewei Zhang ◽  
Z.-Y. Cheng
Keyword(s):  
Detection Limit ◽  
Acoustic Wave ◽  
Resonance Frequency ◽  
Bacillus Anthracis ◽  
High Performance ◽  
Filamentous Phage ◽  
Q Value ◽  
High Q ◽  
Bacillus Anthracis Spores ◽  
Higher Sensitivity

ABSTRACTRecently, the magnetostrictive microcantilever (MSMC) as a high performance biosensor platform was introduced. The MSMC is a wireless acoustic wave (AW) sensor and exhibits a high Q value. More importantly, the MSMC works well in liquid. In this paper, the detection of Bacillus anthracis spores using MSMCs with filamentous phage as the bioprobe is reported. The phased-coated MSMC biosensors were exposed to cultures containing target spores with increasing concentrations ranging from 5 × 104 to 5 × 108 spores/mL. By monitoring the shift in the resonance frequency of the MSMCs, the spores were detected in a real-time manner and a detection limit of 105 spores/mL was obtained for the MSMCs used in this research. Higher sensitivity is expected for the MSMCs with smaller size.

Microfabricated, Wireless, Magnetoelastic Micro-Particles for the Detection of Bacillus Anthracis Spores

2006 ◽  
Vol 951 ◽  
Author(s):  
Jiehui Wan ◽  
Michael L. Johnson ◽  
Huihua Shu ◽  
Valery A. Petrenko ◽  
Bryan A. Chin
Keyword(s):  
Resonance Frequency ◽  
Bacillus Anthracis ◽  
Biomolecular Recognition ◽  
Sensor Surface ◽  
Recognition Element ◽  
Specific Phage ◽  
Micro Particles ◽  
Bacillus Anthracis Spores ◽  
Molecular Recognition Element ◽  
Magnetoelastic Resonance

ABSTRACTMagnetoelastic resonance biosensors were fabricated by immobilizing a bio-molecular recognition element onto the surface of Fe79B21 magnetoelastic particles (MEP). These sensors can be measured wirelessly and remotely for both in-air and in-liquid bacteria detection. Filamentous bacterio-phage that was selected specifically for the detection of Bacillus anthracis spores was employed as the biomolecular recognition element and immobilized onto the MEPs' surfaces. Attachment of the spores to the sensor surface due to specific phage-spore binding results in a shift in the resonance frequency of the biosensor. Insitu measurement of the resonance frequency of biosensors of 5×100×500 microns were used to determine the sensor response as a function of spore concentrations of 103 to 108 cfu/ml. Specificity of the sensor was evaluated by conducting tests using a mixture of Bacillus anthracis Sterne strain, Bacillus cereus and Bacillus megaterium spores.

scholarly journals Enhanced tunable performance of high Q-factor BaxSr1−xTiO3 film bulk acoustic wave resonators

2013 ◽  
Vol 5 (3) ◽  
pp. 361-369 ◽  
Author(s):  
Andrei Vorobiev ◽  
Spartak Gevorgian
Keyword(s):  
Acoustic Wave ◽  
Resonance Frequency ◽  
Coupling Coefficient ◽  
Piezoelectric Effect ◽  
Bulk Acoustic Wave ◽  
Electromechanical Coupling Coefficient ◽  
Q Factor ◽  
High Q ◽  
Series Resonance ◽  
Film Bulk

Emerging intrinsically tunable film bulk acoustic wave(BAW) resonators allow the development of new generation reconfigurable and agile microwave circuits. In this paper, we demonstrate the enhancement of tunable performance of the high Q-factor BaxSr1−xTiO3 BAW – solidly mounted resonators (BAW–SMR) by varying Ba concentration. The Ba0.5Sr0.5TiO3 BAW–SMR reveal tunability of series resonance frequency up to 2.4%, electromechanical coupling coefficient up to 7.5% and rather high Q-factor, up to 250 at 5.3 GHz. Correlations between the measured electroacoustic parameters are analyzed using the theory of dc field-induced piezoelectric effect in paraelectric phase ferroelectrics. Higher coupling coefficient and tunability of resonance frequency of the Ba0.5Sr0.5TiO3 BAW–SMR are associated with higher tunability of permittivity. Strong anisotropy in field-induced piezoelectric effect is predicted with highest coupling coefficient in (001) direction of the BaxSr1−xTiO3 films. It is also shown that the tunability of series resonance frequency of Ba0.5Sr0.5TiO3 BAW–SMR is limited by relatively high and negative nonlinear electrostriction coefficient which is found to be m ≈ −4·1010 m/F. The BAW–SMR Q-factor is limited significantly by extrinsic acoustic loss associated with wave scattering at reflection from relatively rough top interface. The results of analysis show possible ways of further improvement of the performance of tunable BAW–SMR.

A Method Detection Limit for Bacillus anthracis Spores in Water Using an Automated Waterborne Pathogen Concentrator

2015 ◽  
Vol 98 (4) ◽  
pp. 1003-1012 ◽  
Author(s):  
Ben Humrighouse ◽  
Adin Pemberton ◽  
Vicente Gallardo ◽  
H.D. Alan Lindquist ◽  
Robert LaBudde
Keyword(s):  
Detection Limit ◽  
Bacillus Anthracis ◽  
Method Detection Limit ◽  
Waterborne Pathogen ◽  
Bacillus Anthracis Spores

Dendritic Cells Endocytose Bacillus Anthracis Spores: Implications for Anthrax Pathogenesis

2005 ◽  
Author(s):  
Katherine C. Brittingham ◽  
Gordon Ruthel ◽  
Rekha G. Panchal ◽  
Claudette L. Fuller ◽  
Wilson J. Ribot
Keyword(s):  
Dendritic Cells ◽  
Bacillus Anthracis ◽  
Bacillus Anthracis Spores

scholarly journals Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(vinyl chloride) Coated Piezoelectric Crystal Immunosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ali Oztuna ◽  
Hasan Nazir ◽  
Mehmet Baysallar
Keyword(s):  
Bacillus Anthracis ◽  
Vinyl Chloride ◽  
Quartz Crystal ◽  
Adhesive Layer ◽  
Antibody Immobilization ◽  
Potential Threat ◽  
Amine Functionalized ◽  
Poly Vinyl Chloride ◽  
Bacillus Anthracis Spores ◽  
Flow Through

Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.

scholarly journals Murine splenocytes produce inflammatory cytokines in a MyD88-dependent response to Bacillus anthracis spores

2007 ◽  
Vol 9 (2) ◽  
pp. 502-513 ◽  
Author(s):  
Ian J. Glomski ◽  
Jörg H. Fritz ◽  
Selina J. Keppler ◽  
Viviane Balloy ◽  
Michel Chignard ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Inflammatory Cytokines ◽  
Murine Splenocytes ◽  
Bacillus Anthracis Spores

A high sensitivity wireless mass-loading surface acoustic wave DNA biosensor

2014 ◽  
Vol 28 (07) ◽  
pp. 1450056 ◽  
Author(s):  
Hua-Lin Cai ◽  
Yi Yang ◽  
Yi-Han Zhang ◽  
Chang-Jian Zhou ◽  
Cang-Ran Guo ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Dna Sequences ◽  
Biological Treatment ◽  
High Performance ◽  
Processing System ◽  
High Sensitivity ◽  
Treatment Method ◽  
Saw Sensor ◽  
Target Dna

In this paper, a surface acoustic wave (SAW) biosensor with gold delay area on LiNbO 3 substrate detecting DNA sequences is proposed. By well-designed device parameters of the SAW sensor, it achieves a high performance for highly sensitive detection of target DNA. In addition, an effective biological treatment method for DNA immobilization and abundant experimental verification of the sensing effect have made it a reliable device in DNA detection. The loading mass of the probe and target DNA sequences is obtained from the frequency shifts, which are big enough in this work due to an effective biological treatment. The experimental results show that the biosensor has a high sensitivity of 1.2 pg/ml/Hz and high selectivity characteristic is also verified by the few responses of other substances. In combination with wireless transceiver, we develop a wireless receiving and processing system that can directly display the detection results.

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