Discovery and Development of OMNiMIPs: One MoNomer Molecularly Imprinted Polymers

2007 ◽  
Vol 1005 ◽  
Author(s):  
David A. Spivak ◽  
Martha Sibrian-Vazquez ◽  
Stephen Houck
Keyword(s):  
Molecular Recognition ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recognition Element ◽  
Molecular Recognition Element ◽  
New Formulation ◽  
Separation Media

AbstractThere is enormous potential for the analytical applications of molecularly imprinted polymers (MIPs); for example, the imprinted polymer sites can function as the molecular recognition element of sensors, immunoassays, and separation media. However, difficulties with formulation variables and the need for empirical optimization have inhibited the widespread use of MIPs by the general scientific community. While investigating new crosslinkers for molecular imprinting, we have recently discovered a much simpler approach to MIP formation which utilizes a single crosslinking monomer, NOBE (N, Obismethacryloyl ethanolamine) in addition to template, solvent and initiator (shown in Scheme 1). We have given this molecular imprinting method the acronym “OMNiMIPs” which stands for one monomer molecularly imprinted polymers. This new formulation eliminates variables such as choice of functional monomer (FM) and crosslinker (XL), the ratio of functional monomer to crosslinker (FM/XL), and the ratio of functional monomer to template which normally complicates MIP design. The affects of OMNiMIP performance variables toward molecular recognition indicate significant differences between these new materials and traditional MIPs formulated with ethyleneglycol dimethacrylate (EGDMA) and methacrylic acid (MAA). These differences and the utility of OMNiMIPs will be discussed.

Development of Improved Crosslinking Monomers for Molecularly Imprinted Materials

2002 ◽  
Vol 723 ◽  
Author(s):  
David A. Spivak ◽  
Martha Sibrian-Vazquez
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Polymer Complex ◽  
Functional Monomer ◽  
Design Synthesis ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Catalytic Sites ◽  
And Performance

AbstractMolecular imprinting involves the self-assembled complexation of a substrate to functional monomers to form a pre-polymer complex which is “locked-in” to place by copolymerization with an excess of crosslinking monomer. Removal of the template leaves binding or catalytic sites that are complementary in size, shape, and functionality to the template. Most of the research in molecularly imprinted materials has focused on choice of substrate or functional monomer of the pre-polymer complex. The cross-linking monomers have primarily been EGDMA or DVB, which are commercially available. Redirecting focus on the design of crosslinking monomers for molecular imprinting, we have developed new classes of crosslinked polymers to optimize the performance of molecularly imprinted polymers. The design of the new crosslinking monomers has followed two strategies: (1) development of new crosslinked materials for formation of the supporting matrix, and (2) development of crosslinking monomers that simultaneously serve as the functional monomer. The details of the design, synthesis, polymerization and performance of these new crosslinking monomers for molecularly imprinted polymers will be reported.

Signalling molecular recognition nanocavities with multiple functional groups prepared by molecular imprinting and sequential post-imprinting modifications for prostate cancer biomarker glycoprotein detection

2020 ◽  
Vol 8 (35) ◽  
pp. 7987-7993
Author(s):  
Tetsuro Saeki ◽  
Eri Takano ◽  
Hirobumi Sunayama ◽  
Yuri Kamon ◽  
Ryo Horikawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Molecular Recognition ◽  
Functional Groups ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Cancer Biomarker ◽  
Glycoprotein P ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Novel sequential post-imprinting modifications were demonstrated on the development of multi-functionalized molecularly imprinted polymers for a biomarker glycoprotein.

scholarly journals Computer-Aided Design of Molecularly Imprinted Polymers for Simultaneous Detection of Clenbuterol and Its Metabolites

Polymers ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 17 ◽  
Author(s):  
Bingcheng Zhang ◽  
Xin Fan ◽  
Dayun Zhao
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Density Functional ◽  
Simultaneous Detection ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Dummy Template ◽  
Computer Aided

Molecular imprinting technology (MIT) offers an effective technique for efficient separation and enrichment of specific analytes from complicated matrices and has been used for illicit veterinary drug detectionin recent years due to its high selectivity, good chemical stability, and simple preparation. The development of in silico-based approaches has enabled the simulation of molecularly imprinted polymers (MIPs) to facilitate the selection of imprinting conditions such as template, functional monomer, and the best suitable solvent. In this work, using density functional theory (DFT), the molecularly imprinted polymers of clenbuterol and its metabolites were designed by computer-aided at B3LYP/6-31 + G (d, p) level. Screening molecular imprinting components such as functional monomers, cross-linkers, and solvents has been achieved in the computational simulation considerations. The simulation results showed that methacrylic acid (MAA) is the best functional monomer; the optimal imprinting ratio for both clenbuterol (CLB) and its dummy template molecule of phenylephrine (PE) to functional monomer is 1:3, while the optimal imprinting ratio for the two dummy template molecules of CLB’s metabolites is 1:5. Choosin gethyleneglycol dimethacrylate (EDGMA) as a crosslinker and aprotic solvents could increase the selectivity of the molecularly imprinted system. Atoms in Molecules (AIM) topology analysis was applied to investigate the template-monomer complexes bonding situation and helped to explain the nature of the reaction in the imprinting process. These theoretical predictions were also verified by the experimental results and found to be in good agreement with the computational results. The computer-simulated imprinting process compensates for the lack of clarity in the mechanism of the molecular imprinting process, and provides an important reference and direction for developing better recognition pattern towards CLB and its metabolite analytes in swine urine samples at the same time.

scholarly journals Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Sensors ◽  
2019 ◽  
Vol 19 (16) ◽  
pp. 3485 ◽  
Author(s):  
Brian Regan ◽  
Fiona Boyle ◽  
Richard O’Kennedy ◽  
David Collins
Keyword(s):  
Cardiovascular Disease ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Point Of Care ◽  
Analytical Performance ◽  
Point Of Care Testing ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recognition Element ◽  
Recognition Elements

Molecular imprinting is a rapidly growing area of interest involving the synthesis of artificial recognition elements that enable the separation of analyte from a sample matrix and its determination. Traditionally, this approach can be successfully applied to small analyte (<1.5 kDa) separation/ extraction, but, more recently it is finding utility in biomimetic sensors. These sensors consist of a recognition element and a transducer similar to their biosensor counterparts, however, the fundamental distinction is that biomimetic sensors employ an artificial recognition element. Molecularly imprinted polymers (MIPs) employed as the recognition elements in biomimetic sensors contain binding sites complementary in shape and functionality to their target analyte. Despite the growing interest in molecularly imprinting techniques, the commercial adoption of this technology is yet to be widely realised for blood sample analysis. This review aims to assess the applicability of this technology for the point-of-care testing (POCT) of cardiovascular disease-related biomarkers. More specifically, molecular imprinting is critically evaluated with respect to the detection of cardiac biomarkers indicative of acute coronary syndrome (ACS), such as the cardiac troponins (cTns). The challenges associated with the synthesis of MIPs for protein detection are outlined, in addition to enhancement techniques that ultimately improve the analytical performance of biomimetic sensors. The mechanism of detection employed to convert the analyte concentration into a measurable signal in biomimetic sensors will be discussed. Furthermore, the analytical performance of these sensors will be compared with biosensors and their potential implementation within clinical settings will be considered. In addition, the most suitable application of these sensors for cardiovascular assessment will be presented.

scholarly journals Molecular Recognition: Perspective and a New Approach

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2757
Author(s):  
W. Rudolf Seitz ◽  
Casey J. Grenier ◽  
John R. Csoros ◽  
Rongfang Yang ◽  
Tianyu Ren
Keyword(s):  
Molecular Recognition ◽  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Binding Kinetics ◽  
Molecularly Imprinted ◽  
New Approach ◽  
Imprinted Polymers ◽  
High Affinity ◽  
Water Blocking ◽  
High Level

This perspective presents an overview of approaches to the preparation of molecular recognition agents for chemical sensing. These approaches include chemical synthesis, using catalysts from biological systems, partitioning, aptamers, antibodies and molecularly imprinted polymers. The latter three approaches are general in that they can be applied with a large number of analytes, both proteins and smaller molecules like drugs and hormones. Aptamers and antibodies bind analytes rapidly while molecularly imprinted polymers bind much more slowly. Most molecularly imprinted polymers, formed by polymerizing in the presence of a template, contain a high level of covalent crosslinker that causes the polymer to form a separate phase. This results in a material that is rigid with low affinity for analyte and slow binding kinetics. Our approach to templating is to use predominantly or exclusively noncovalent crosslinks. This results in soluble templated polymers that bind analyte rapidly with high affinity. The biggest challenge of this approach is that the chains are tangled when the templated polymer is dissolved in water, blocking access to binding sites.

Signaling molecularly imprinted polymers: molecular recognition-based sensing materials

2005 ◽  
Vol 5 (5) ◽  
pp. 263-275 ◽  
Author(s):  
Toshifumi Takeuchi ◽  
Takashi Mukawa ◽  
Hideyuki Shinmori
Keyword(s):  
Molecular Recognition ◽  
Molecularly Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Fluorescence signaling molecularly imprinted polymers for antibiotics prepared via site-directed post-imprinting introduction of plural fluorescent reporters within the recognition cavity

2016 ◽  
Vol 4 (44) ◽  
pp. 7138-7145 ◽  
Author(s):  
Hirobumi Sunayama ◽  
Takeo Ohta ◽  
Atsushi Kuwahara ◽  
Toshifumi Takeuchi
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Fluorescent Dyes ◽  
Specific Binding ◽  
Fluorescence Signal ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Fluorescent Reporters ◽  
Binding Event ◽  
Fluorescence Signaling

An antibiotic-imprinted cavity with two different fluorescent dyes was prepared by molecular imprinting and subsequent post-imprinting modifications (PIMs), for the readout of a specific binding event as a fluorescence signal.

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