Fluorescence signaling molecularly imprinted polymers for antibiotics prepared via site-directed post-imprinting introduction of plural fluorescent reporters within the recognition cavity

2016 ◽  
Vol 4 (44) ◽  
pp. 7138-7145 ◽  
Author(s):  
Hirobumi Sunayama ◽  
Takeo Ohta ◽  
Atsushi Kuwahara ◽  
Toshifumi Takeuchi
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Fluorescent Dyes ◽  
Specific Binding ◽  
Fluorescence Signal ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Fluorescent Reporters ◽  
Binding Event ◽  
Fluorescence Signaling

An antibiotic-imprinted cavity with two different fluorescent dyes was prepared by molecular imprinting and subsequent post-imprinting modifications (PIMs), for the readout of a specific binding event as a fluorescence signal.

scholarly journals Cholesterol templated polymers : are they really specific binding materials?

2021 ◽  
Author(s):  
Konstance Tsavdaris
Keyword(s):  
Endocrine Disruptors ◽  
Inclusion Complexes ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Specific Binding ◽  
Molecularly Imprinted ◽  
Molecularly Imprinting ◽  
Imprinted Polymers ◽  
Starting Point

The technique of molecular imprinting creates template specific and selective polymer products with a large assortment of applications. For example, molecularly imprinted polymers (MIPs) may pose as a means to separate undesirable components, such as endocrine disruptors, from the environment. Taking advantage of β-cyclodextrin's abililty to form inclusion complexes with a number of guests has led scientists to use them as scaffolds in the synthesis of MIPs. Komiyana's approach of molecularly imprinting cholesterol with β-cyclodextrin was used as a starting point to apply MIPs as potential tools for trapping endocrine disruptors. This study presents results on the re-binding of cholesterol to a cholesterol-templated MIP and to a non-MIP (NMIP), as well as the binding of a series of structurally unrelated compounds to the cholesterol-templated MIP and NMIP. The results consistently show that cholesterol-templated MIPs synthesized using Komiyama's method lack specificity and selectivity for their template. This calls into question their efficacy as a tool for trapping endocrine disruptors.

scholarly journals Cholesterol templated polymers : are they really specific binding materials?

2021 ◽  
Author(s):  
Konstance Tsavdaris
Keyword(s):  
Endocrine Disruptors ◽  
Inclusion Complexes ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Specific Binding ◽  
Molecularly Imprinted ◽  
Molecularly Imprinting ◽  
Imprinted Polymers ◽  
Starting Point

The technique of molecular imprinting creates template specific and selective polymer products with a large assortment of applications. For example, molecularly imprinted polymers (MIPs) may pose as a means to separate undesirable components, such as endocrine disruptors, from the environment. Taking advantage of β-cyclodextrin's abililty to form inclusion complexes with a number of guests has led scientists to use them as scaffolds in the synthesis of MIPs. Komiyana's approach of molecularly imprinting cholesterol with β-cyclodextrin was used as a starting point to apply MIPs as potential tools for trapping endocrine disruptors. This study presents results on the re-binding of cholesterol to a cholesterol-templated MIP and to a non-MIP (NMIP), as well as the binding of a series of structurally unrelated compounds to the cholesterol-templated MIP and NMIP. The results consistently show that cholesterol-templated MIPs synthesized using Komiyama's method lack specificity and selectivity for their template. This calls into question their efficacy as a tool for trapping endocrine disruptors.

scholarly journals MOLECULARLY IMPRINTED POLYMER NANOPARTICLES (MIP-NPs) APPLICATIONS IN ELECTROCHEMICAL SENSORS

2019 ◽  
pp. 1-6
Author(s):  
DIANE FAUZI ◽  
FEBRINA AMELIA SAPUTRI
Keyword(s):  
Molecularly Imprinted Polymers ◽  
High Stability ◽  
Electrochemical Sensors ◽  
Specific Binding ◽  
Environmental Contaminants ◽  
Polymer Nanoparticles ◽  
Selective Recognition ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Molecularly Imprinted Polymers (MIPs) is a polymer that binds together to form a specific binding site that is selective for certain analytes. Its high stability, its synthesize simplicity, and it can ease costs significantly make it was applied widely as a receptor instead of antibodies or enzymes. MIPs can be re-developed into MIPs nanoparticles (MIP-NPs) which have greater potential. MIPs use in electrochemical sensors have relevant applications in daily life and have been tested in human samples. Electrochemical sensors have been successfully functioned with MIP-NPs leading to real-time monitoring of drugs, pesticides, environmental contaminants, and secondary metabolites, as well as molecules with biological relevance. The aim of this review is to summarize the developments and applications of MIP-NPs as a selective recognition component in electrochemical sensors with special emphasis on their analytical applications.

scholarly journals Smart Bandage Based on Molecularly Imprinted Polymers (MIPs) for Diclofenac Controlled Release

2018 ◽  
Vol 11 (4) ◽  
pp. 92 ◽  
Author(s):  
Ortensia Parisi ◽  
Mariarosa Ruffo ◽  
Luca Scrivano ◽  
Rocco Malivindi ◽  
Antonio Vassallo ◽  
...  
Keyword(s):  
Controlled Release ◽  
Molecularly Imprinted Polymers ◽  
Specific Binding ◽  
Skin Irritation ◽  
Spherical Particles ◽  
Batch Adsorption ◽  
Scatchard Analysis ◽  
Binding Studies ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

The aim of the present study was the development of a “smart bandage” for the topical administration of diclofenac, in the treatment of localized painful and inflammatory conditions, incorporating Molecularly Imprinted Polymers (MIPs) for the controlled release of this anti-inflammatory drug. For this purpose, MIP spherical particles were synthesized by precipitation polymerization, loaded with the therapeutic agent and incorporated into the bandage surface. Batch adsorption binding studies were performed to investigate the adsorption isotherms and kinetics and the selective recognition abilities of the synthesized MIP. In vitro diffusion studies were also carried out using Franz cells and the obtained results were reported as percentage of the diffused dose, cumulative amount of diffused drug, steady-state drug flux and permeability coefficient. Moreover, the biocompatibility of the developed device was evaluated using the EPISKIN™ model. The Scatchard analysis indicated that the prepared MIP is characterized by the presence of specific binding sites for diclofenac, which are not present in the corresponding non-imprinted polymer, and the obtained results confirmed both the ability of the prepared bandage to prolong the drug release and the absence of skin irritation reactions. Therefore, these results support the potential application of the developed “smart bandage” as topical device for diclofenac sustained release.

Advances in Molecularly Imprinted Systems: Materials, Characterization Methods and Analytical Applications

2020 ◽  
Vol 16 (3) ◽  
pp. 196-207 ◽  
Author(s):  
Yeşeren Saylan ◽  
Adil Denizli
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
High Stability ◽  
Low Cost ◽  
Three Dimensional ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Wide Range ◽  
Physicochemical Features ◽  
Target Molecules

Introduction: A molecular imprinting is one of the fascinating modification methods that employ molecules as targets to create geometric cavities for recognition of targets in the polymeric matrix. This method provides a broad versatility to imprint target molecules with different size, three-dimensional structure and physicochemical features. In contrast to the complex and timeconsuming laboratory surface modification procedures, this method offers a rapid, sensitive, inexpensive, easy-to-use, and selective approach for the diagnosis, screening and monitoring disorders. Owing to their unique features such as high selectivity, physical and chemical robustness, high stability, low-cost and reusability of this method, molecularly imprinted polymers have become very attractive materials and been applied in various applications from separation to detection. Background: The aims of this review are structured according to the fundamentals of molecularly imprinted polymers involving essential elements, preparation procedures and also the analytical applications platforms. Finally, the future perspectives to increase the development of molecularly imprinted platforms. Methods: A molecular imprinting is one of the commonly used modification methods that apply target as a recognition element itself and provide a wide range of versatility to replica other targets with a different structure, size, and physicochemical features. A rapid, easy, cheap and specific recognition approach has become one of the investigation areas on, especially biochemistry, biomedicine and biotechnology. In recent years, several technologies of molecular imprinting method have gained prompt development according to continuous use and improvement of traditional polymerization techniques. Results: The molecularly imprinted polymers with excellent performances have been prepared and also more exciting and universal applications have been recognized. In contrast to the conventional methods, the imprinted systems have superior advantages including high stability, relative ease and low cost of preparation, resistance to elevated temperature, and pressure and potential application to various target molecules. In view of these considerations, molecularly imprinted systems have found application in various fields of analytical chemistry including separation, purification, detection and spectrophotometric systems. Conclusion: Recent analytical methods are reported to develop the binding kinetics of imprinted systems by using the development of other technologies. The combined platforms are among the most encouraging systems to detect and recognize several molecules. The diversity of molecular imprinting methods was overviewed for different analytical application platforms. There is still a requirement of more knowledge on the molecular features of these polymers. A next step would further be the optimization of different systems with more homogeneous and easily reachable recognition sites to reduce the laborious in the accessibility in the three-dimensional polymeric materials in sufficient recognition features and also better selectivity and sensitivity for a wide range of molecules.

The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food

2017 ◽  
Vol 9 (47) ◽  
pp. 6682-6688 ◽  
Author(s):  
Jing Wang ◽  
Yaxin Sang ◽  
Weihua Liu ◽  
Na Liang ◽  
Xianghong Wang
Keyword(s):  
Immunosorbent Assay ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Molecular Imprinting Technique

In this study, a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method using molecularly imprinted polymers (MIPs) as artificial antibodies was developed to detect enrofloxacin (ENRO) in animal-based food.

Development of Improved Crosslinking Monomers for Molecularly Imprinted Materials

2002 ◽  
Vol 723 ◽  
Author(s):  
David A. Spivak ◽  
Martha Sibrian-Vazquez
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Polymer Complex ◽  
Functional Monomer ◽  
Design Synthesis ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Catalytic Sites ◽  
And Performance

AbstractMolecular imprinting involves the self-assembled complexation of a substrate to functional monomers to form a pre-polymer complex which is “locked-in” to place by copolymerization with an excess of crosslinking monomer. Removal of the template leaves binding or catalytic sites that are complementary in size, shape, and functionality to the template. Most of the research in molecularly imprinted materials has focused on choice of substrate or functional monomer of the pre-polymer complex. The cross-linking monomers have primarily been EGDMA or DVB, which are commercially available. Redirecting focus on the design of crosslinking monomers for molecular imprinting, we have developed new classes of crosslinked polymers to optimize the performance of molecularly imprinted polymers. The design of the new crosslinking monomers has followed two strategies: (1) development of new crosslinked materials for formation of the supporting matrix, and (2) development of crosslinking monomers that simultaneously serve as the functional monomer. The details of the design, synthesis, polymerization and performance of these new crosslinking monomers for molecularly imprinted polymers will be reported.

Signalling molecular recognition nanocavities with multiple functional groups prepared by molecular imprinting and sequential post-imprinting modifications for prostate cancer biomarker glycoprotein detection

2020 ◽  
Vol 8 (35) ◽  
pp. 7987-7993
Author(s):  
Tetsuro Saeki ◽  
Eri Takano ◽  
Hirobumi Sunayama ◽  
Yuri Kamon ◽  
Ryo Horikawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Molecular Recognition ◽  
Functional Groups ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Cancer Biomarker ◽  
Glycoprotein P ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Novel sequential post-imprinting modifications were demonstrated on the development of multi-functionalized molecularly imprinted polymers for a biomarker glycoprotein.

Water-compatible molecularly imprinted polymers prepared using metal–organic gel as porogen

RSC Advances ◽  
2015 ◽  
Vol 5 (103) ◽  
pp. 84601-84609 ◽  
Author(s):  
Li Ma ◽  
Lei Tang ◽  
Rong-Shan Li ◽  
Yan-Ping Huang ◽  
Zhao-Sheng Liu
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Metal Organic ◽  
Organic Gel

A novel water-compatible approach suitable for molecular imprinting was described by using metal–organic gel (MOG) as the porogenic solvent.

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