Culture of Insect Heart Muscle Tissue and Its Applicability to Bio-Actuators

2008 ◽  
Vol 1096 ◽  
Author(s):  
Yoshitake Akiyama ◽  
Kikuo Iwabuchi ◽  
Yuji Furukawa ◽  
Keisume Morishima
Keyword(s):  
Muscle Tissue ◽  
Heart Muscle ◽  
Average Frequency ◽  
Culture Conditions ◽  
Electrical Pulse ◽  
Mammalian Tissue ◽  
Dorsal Vessel ◽  
Tetanic Contraction ◽  
Twitch Contraction ◽  
Regulation Method

AbstractAn insect heart (dorsal vessel) is well suited as an environmentally robust bioactuator since insect tissue is generally robust over culture conditions compared with mammalian tissue. In this paper, the applicability of a caterpillar dorsal vessel to a bioactuator was assessed by fabricating a micropillar actuator driven by dorsal vessel tissue and evaluating the response to electrical pulse stimuli. The actuator worked autonomously for more than 90 days at 25 °C without any maintenance. The average frequency and displacement for 30 s on the 28th day of culturing were 0.83 Hz and 41 μm, respectively. Furthermore, as a regulation method for the dorsal vessel, electrical pulse stimuli were applied to the micropillar actuator. The contractile delay was about 50 ms. A twitch contraction was evoked by electrical pulse stimulus at 20 ms in duration and 10 volts in amplitude. A tetanic contraction was observed when stimuli over 10 Hz were applied.

Tetanic Contraction in Vocal Fold Muscle

1989 ◽  
Vol 32 (2) ◽  
pp. 226-231 ◽  
Author(s):  
Fariborz Alipour-Haghighi ◽  
Ingo R. Titze ◽  
Adrienne L. Perlman
Keyword(s):  
Muscle Tissue ◽  
Isometric Tension ◽  
Tetanic Contraction ◽  
Shortening Velocity ◽  
Twitch Contraction ◽  
Passive Response ◽  
Recorded Data ◽  
Series Of Experiments ◽  
Isotonic Shortening

Active properties of canine vocalis muscle tissue were investigated through a series of experiments conducted in vitro. Samples of the vocalis muscle were dissected from dog larynges excised a few minutes before death and kept in Krebs solution at a temperature of 37 ± 1°C and a pH of 7.4 ± 0.05. Isometric and isotonic tetanic responses of the vocalis muscle were obtained electronically with a Dual Servo System (ergometer). Isometric tension was recorded at various levels of elongation and stimulation rate. Isotonic shortening was recorded at various levels of force, and shortening velocity was obtained by numerical analysis of recorded data. It was found that fused tetanus occurred at stimulation rates of about 90 Hz, where the isometric titanic force saturates. Repeated stimulation of the muscle in vitro not only caused nonrecoverable fatigue in the tissue, but also decreased its passive tension. The combined active and passive isometric tension increased with elongation of the muscle. Results of isometric active responses were normalized with respect to average passive response. This normalization allowed for better comparison between tetanic contraction and twitch contraction. It was found that maximum tetanic contraction was 6.4 times greater than maximum twitch contraction obtained in a previous study. A tetanic contraction period was defined and investigated for eight samples of vocalis muscle tissue from different dogs. The tetanic contraction period showed a linear increasing trend with strain.

Flavo-Protein of the Heart Muscle Tissue

Nature ◽  
1939 ◽  
Vol 143 (3611) ◽  
pp. 76-77 ◽  
Author(s):  
F. B. STRAUB
Keyword(s):  
Muscle Tissue ◽  
Heart Muscle

scholarly journals Isolation and properties of a flavoprotein from heart muscle tissue

1939 ◽  
Vol 33 (5) ◽  
pp. 787-792 ◽  
Author(s):  
Ferenc Bruno Straub
Keyword(s):  
Muscle Tissue ◽  
Heart Muscle

scholarly journals Heart phantom with electrical properties of heart muscle tissue

2018 ◽  
Vol 4 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Leonie Korn ◽  
Simon Lyra ◽  
Daniel Rüschen ◽  
Alexander Pugovkin ◽  
Dmitry Telyshev ◽  
...  
Keyword(s):  
Electrical Properties ◽  
Muscle Tissue ◽  
Heart Muscle ◽  
Casting Process ◽  
Left Ventricular ◽  
Myocardial Tissue ◽  
Ventricular Assist ◽  
Novel Method ◽  
Materials Used ◽  
Silicone Model

AbstractThe weakened heart is supported by a left ventricular assist device (LVAD) to supply the heart muscle with oxygenated blood. In case the heart muscle recovers during LVAD therapy, the patient has to be weaned from the device. To date, there is no adequate method to detect heart muscle recovery in LVAD therapy. In order to establish a novel method based on the measurement of electric conductivity, this study presents a silicone model of a ventricle mock-up to simulate the electrical properties of cardiac muscle tissue. Previously, it has been shown that the electrical properties of myocardial tissue change during ischemia, so that these changes are a possible estimate for measuring the condition of myocardial tissue. To this purpose, this study presents a casting process for a ventricle model and describes the materials used to imitate the electrical properties of the heart muscle to obtain conductive material. Initial results showed that the higher the carbon concentration in the silicone, the higher the conductivity of the silicone samples. The measurements were performed at different frequencies and the samples were analyzed for homogenization.

scholarly journals Effects of Synthetic Anti-Inflammatory Sterol in CB3V-Induced Myocarditis: A Morphological Study on Heart Muscle Tissue

2016 ◽  
Vol 1 (1) ◽  
pp. 69-89 ◽  
Author(s):  
Paola Castrogiovanni ◽  
Francesca Trovato ◽  
Marta Szychlinska ◽  
Carla Loreto ◽  
Salvatore Giunta ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Heart Muscle ◽  
Morphological Study ◽  
Anti Inflammatory

scholarly journals 4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

2017 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
Gohar Musheghyan ◽  
Arevik Minasyan ◽  
Gohar Arajyan ◽  
Sinerik Ayrapetyan
Keyword(s):  
Brain Tissue ◽  
Muscle Tissue ◽  
Heart Muscle ◽  
Pain Threshold ◽  
Mechanical Vibration ◽  
Membrane Receptors ◽  
Tissue Hydration ◽  
Thermal Pain ◽  
Muscle Tissues

In this work the effect of 4Hz 30dB horizontal mechanical vibration (MV) on thermal pain threshold, hydration and [3H]-ouabain binding in brain and heart muscle tissues of rats was studied. It was revealed that 4Hz MV treatment for 10 minutes increased pain threshold, which was accompanied by brain and heart muscle tissue dehydration. In vitro state, hydration of brain and heart muscle tissues of sham animals was increased, while in 4Hz MV-treated animals the increase of brain tissue hydration was more pronounced and heart muscle tissues were dehydrated. The fact that 4Hz MV treatment also impacted heart muscle tissue hydration indicates that 4Hz MV effect on brain and heart muscle tissues is realized through a common messenger circulating in blood. The incubation of brain and heart muscle tissues in PS containing 10-4M and 10-9M ouabain led to tissue hydration in sham and 4Hz MV-treated animals. However, tissues of 4Hz MV-treated animals were less hydrated, and this hydration was accompanied by the decrease and increase of membrane receptors’ affinity at 10-4M and 10-9M ouabain concentrations, respectively. Based on the obtained data, it is suggested that pain-relieving effect of 4Hz MV is due to α3 isoform-dependent brain tissue dehydration.

Effects of acidosis on Ca2+sensitivity of contractile elements in intact ferret myocardium

1998 ◽  
Vol 274 (1) ◽  
pp. H147-H154 ◽  
Author(s):  
Kimiaki Komukai ◽  
Tetsuya Ishikawa ◽  
Satoshi Kurihara
Keyword(s):  
Muscle Length ◽  
Length Change ◽  
Troponin C ◽  
Tetanic Contraction ◽  
Twitch Contraction ◽  
Intracellular Ca ◽  
The Right ◽  
The Hill ◽  
The Relationship ◽  
Stimulation Of

We investigated the effects of acidosis on the intracellular Ca2+ concentration ([Ca2+]i) and contractile properties of intact mammalian cardiac muscle during tetanic and twitch contractions. Aequorin was injected into ferret papillary muscles, and the [Ca2+]iand tension were simultaneously measured. Acidosis was attained by increasing the CO2 concentration in the bicarbonate (20 mM)-buffered Tyrode solution from 5% (pH 7.35, control) to 15% (pH 6.89, acidosis). Tetanic contraction was produced by repetitive stimulation of the preparation following treatment with 5 μM ryanodine. The relationship between [Ca2+]iand tension was measured 6 s after the onset of the stimulation and was fitted using the Hill equation. Acidosis decreased the maximal tension to 81 ± 2% of the control and shifted the [Ca2+]i-tension relationship to the right by 0.18 ± 0.01 pCa units. During twitch contraction, a quick shortening of muscle length from the length at which developed tension became maximal ( L max) to 92% L maxproduced a transient change in the [Ca2+]i(extra Ca2+). The magnitude of the extra Ca2+ was dependent on the [Ca2+]iimmediately before the length change, suggesting that the extra Ca2+ is related to the amount of troponin-Ca complex. Acidosis decreased the normalized extra Ca2+ to [Ca2+]iimmediately before the length change, which indicates that the amount of Ca2+ bound to troponin C is less when [Ca2+]iis the same as in the control. The decrease in the Ca2+ binding to troponin C explains the decrease in tetanic and twitch contraction, and mechanical stress applied to the preparation induced less [Ca2+]ichange in acidosis.

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