Elastolytic-sensitive 3D-printed chitosan scaffold for wound healing applications

AbstractDeveloping an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.

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N-halamine modified ceria nanoparticles: Antibacterial response and accelerated wound healing application via a 3D printed scaffold

Composites Part B Engineering ◽

10.1016/j.compositesb.2021.109390 ◽

2021 ◽

Vol 227 ◽

pp. 109390

Author(s):

Zhenming Yang ◽

Xuehong Ren ◽

Yu Liu

Keyword(s):

Wound Healing ◽

Ceria Nanoparticles ◽

3D Printed

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PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing

Scientific Reports ◽

10.1038/s41598-019-55214-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 6

Author(s):

Amritha Vijayan ◽

Sabareeswaran A. ◽

G. S. Vinod Kumar

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Growth Factor ◽

Treated Group ◽

Growth Factor Delivery ◽

Chitosan Scaffold ◽

Collagen Formation ◽

Dual Growth Factor Delivery

AbstractApplication of growth factors at wound site has improved the efficiency and quality of healing. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) induce proliferation of various cells in wound healing. Delivery of growth factor from controlled release systems protect it from degradation and also result in sustained delivery of it at the site of injury. The goal of the study was to develop a Polyethylene glycol (PEG) cross-linked cotton-like chitosan scaffold (CS-PEG-H) by freeze-drying method and chemically conjugate heparin to the scaffold to which the growth factors can be electrostatically bound and evaluate its wound healing properties in vitro and in vivo. The growth factor containing scaffolds induced increased proliferation of HaCaT cells, increased neovascularization and collagen formation seen by H and E and Masson’s trichrome staining. Immunohistochemistry was performed using the Ki67 marker which increased proliferation of cells in growth factor containing scaffold treated group. Frequent dressing changes are a major deterrent to proper wound healing. Our system was found to release both VEGF and bFGF in a continuous manner and attained stability after 7 days. Thus our system can maintain therapeutic levels of growth factor at the wound bed thereby avoiding the need for daily applications and frequent dressing changes. Thus, it can be a promising candidate for wound healing.

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3D Printed Pericardium Hydrogels To Promote Wound Healing in Vascular Applications

Biomacromolecules ◽

10.1021/acs.biomac.7b01165 ◽

2017 ◽

Vol 18 (11) ◽

pp. 3802-3811 ◽

Cited By ~ 20

Author(s):

Laura G. Bracaglia ◽

Michael Messina ◽

Shira Winston ◽

Che-Ying Kuo ◽

Max Lerman ◽

...

Keyword(s):

Wound Healing ◽

Promote Wound Healing ◽

3D Printed

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Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold

PLoS ONE ◽

10.1371/journal.pone.0099410 ◽

2014 ◽

Vol 9 (6) ◽

pp. e99410 ◽

Cited By ~ 65

Author(s):

Ken Ye ◽

Raed Felimban ◽

Kathy Traianedes ◽

Simon E. Moulton ◽

Gordon G. Wallace ◽

...

Keyword(s):

Stem Cells ◽

Adipose Stem Cells ◽

Infrapatellar Fat Pad ◽

Fat Pad ◽

Chitosan Scaffold ◽

3D Printed

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Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Journal of Tissue Engineering ◽

10.1177/20417314211063022 ◽

2022 ◽

Vol 13 ◽

pp. 204173142110630

Author(s):

Peng Chang ◽

Shijie Li ◽

Qian Sun ◽

Kai Guo ◽

Heran Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Wound Healing ◽

Functional Unit ◽

Collagen Gel ◽

Skin Wound ◽

Full Thickness ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin ◽

3D Printed

Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing.

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