DNase I functional microgels for neutrophil extracellular trap disruption

Non-fouling DNase I conjugated microgel provide a novel biohybrid platform to disrupt Neutrophil extracellular traps (NETs) and can be used as a non-thrombogenic coating for reduction of NET-mediated inflammation and microthrombi formation.

Bioinspired dopamine and zwitterionic polymers for non-fouling surface engineering

Conjugation of zwitterionic polymer with dopamine is a promising approach to endow the surface with antifouling properties for medical devices, biosensors and marine coating applications etc. Zwitterionic polymers can be conjugated with dopamine by mostly four methods.

Blood Retention and Antigenicity of Polycarboxybetaine-Modified Liposomes

<div>Zwitterionic polycarboxybetaines (PCBs) have gained attention as alternative stealth polymers whose</div><div>liposomal formulation and protein conjugates were reported not to elicit anti-polymer antibodies. Here, we</div><div>studied the blood retention and antigenicity of liposomes modified with PCBs focusing on their chemical</div><div>structures and doses. We compared PCBs with either 1 or 3 (PCB1 or PCB3) spacer carbons between the</div><div>carboxylate and ammonium groups. PCB3-modified liposomes had a short blood retention, whereas PCB1-</div><div>modified liposomes demonstrated extended blood retention that was somewhat superior to PEGylated liposome.</div><div>This confirmed the excellent non-fouling nature of PCB1 reported previously. Interestingly, PCB1-liposome as</div><div>well as PCB3-liposome elicited specific IgMs toward each PCB. The dose-dependent production of specific</div><div>IgMs to PCB-liposomes was similar to that of PEGylated liposome, i.e., high doses of PCB-liposomes reduced</div><div>the production of specific IgMs, termed immunological tolerance. These results indicate the importance of</div><div>investigating the effect of dose to clarify the existence of antigenicity of stealth polymers</div>

Blood Retention and Antigenicity of Polycarboxybetaine-Modified Liposomes

<div>Zwitterionic polycarboxybetaines (PCBs) have gained attention as alternative stealth polymers whose</div><div>liposomal formulation and protein conjugates were reported not to elicit anti-polymer antibodies. Here, we</div><div>studied the blood retention and antigenicity of liposomes modified with PCBs focusing on their chemical</div><div>structures and doses. We compared PCBs with either 1 or 3 (PCB1 or PCB3) spacer carbons between the</div><div>carboxylate and ammonium groups. PCB3-modified liposomes had a short blood retention, whereas PCB1-</div><div>modified liposomes demonstrated extended blood retention that was somewhat superior to PEGylated liposome.</div><div>This confirmed the excellent non-fouling nature of PCB1 reported previously. Interestingly, PCB1-liposome as</div><div>well as PCB3-liposome elicited specific IgMs toward each PCB. The dose-dependent production of specific</div><div>IgMs to PCB-liposomes was similar to that of PEGylated liposome, i.e., high doses of PCB-liposomes reduced</div><div>the production of specific IgMs, termed immunological tolerance. These results indicate the importance of</div><div>investigating the effect of dose to clarify the existence of antigenicity of stealth polymers</div>