scholarly journals OPTIMASI PERFORMA DNA MARKER PADA ELEKTROFORESIS GEL

2017 ◽  
Vol 15 (2) ◽  
pp. 47
Author(s):  
Diah Artati ◽  
Dini Sahfitri Lubis
Keyword(s):  
Deoxyribonucleic Acid ◽  
Dna Marker

Elektroforesis adalah pemisahan komponen bermuatan listrik berdasarkan perbedaan tingkat migrasinya dalam suatu medan listrik. Elektroforesis terutama digunakan untuk mengamati hasil dari DNA (deoxyribonucleic acid) yang diamplifikasi. Untuk memudahkan pembacaan ukuran DNA target dalam elektroforesis diperlukan adanya DNA marker. Kendala-kendala penggunaan DNA maker dalam elektroforesis di antaranya adalah separasi fragmen DNA marker yang kurang sempurna, fragmen yang tipis atau kurang tegas dan bentuk fragmen yang tidak lurus, sehingga menyulitkan pembacaan. Oleh karena itu, perlu dilakukan uji coba dengan memberikan perlakuan beberapa faktor yang memengaruhi hasil elektroforesis untuk mengetahui komposisi yang tepat agar diperoleh performa fragmen DNA marker yang optimum. Kegiatan optimasi performa DNA marker ini dilakukan dengan cara memasukkan sampel DNA marker dengan volume 0,50 µL; 0,75 µL; 1,00 µL; 1,25 µL; dan 1,50 µL ke dalam sumur-sumur gel agarosa 2% yang telah diwarnai peqGREEN dengan konsentrasi 60.000x in water (0,5 µL/30 mL), 40.000x in water (0,75 µL/30 mL), 30.000x in water (1 µL/30 mL), dan 24.000x in water (1,25 µL/30 mL), kemudian dilakukan elektroforesis pada arus listrik 400 mA dengan tegangan listrik 80 volt selama 30 menit, 70 volt selama 45 menit, dan 60 volt selama 60 menit. Hasil uji coba ini menunjukkan bahwa volume optimum DNA marker yang dapat menghasilkan visualisasi fragmen-fragmen DNA secara jelas berkisar 0,75-1,25 µL. Pewarna peqGREEN dengan konsentrasi 60.000x in water sudah dapat mewarnai fragmen DNA dengan jelas. Separasi antar-fragmen DNA terlihat jelas dan tegas pada saat elektroforesis dilakukan dengan tegangan listrik 70 volt selama 45 menit dan 60 volt selama 60 menit.

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

DNA MARKER STUDY OF RCG1 LOCUS IN THE GRAPE GENOTYPES

2019 ◽  
Vol 23 ◽  
pp. 40-44
Author(s):  
E.T. Ilnitskaya ◽  
◽  
M.V. Makarkina ◽  
S.V. Tokmakov ◽  
◽  
...  
Keyword(s):  
Dna Marker ◽  
Marker Study

STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA)

EUGENIA ◽  
2008 ◽  
Vol 14 (1) ◽  
Author(s):  
Semuel D. Runtunuwu ◽  
Hengky Novarianto ◽  
Heldering Tampake ◽  
Edy F. Lengkong
Keyword(s):  
Dna Marker ◽  
Rapd Marker ◽  
High Yield ◽  
Randomly Amplified Polymorphic Dna ◽  
Germination Time ◽  
Flower Production ◽  
Short Time

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.

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