Faculty Opinions recommendation of Effects of cryopreservation on human sperm deoxyribonucleic acid integrity.

2010 ◽  
Author(s):  
Michael Witt
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Sperm

scholarly journals Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches.

1977 ◽  
Vol 25 (7) ◽  
pp. 763-773 ◽  
Author(s):  
M A Van Dilla ◽  
B L Gledhill ◽  
S Lake ◽  
P N Dean ◽  
J W Gray ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Sperm ◽  
Test System ◽  
Index Of Refraction ◽  
Lateral Extension ◽  
Mammalian Sperm ◽  
Y Chromosomes ◽  
Coefficients Of Variation ◽  
Flat Shape ◽  
The Right

Measurement of mammalian sperm deoxyribonucleic acid content is of importance in several areas of biomedical research. When measured in flow systems with orthogonal axes of illumination, flow and detection, an unexpected, distorted distribution consisting of a narrow peak with a lateral extension to the right is observed. Several lines of evidence lead to the conclusion that this effect is an optical-geometric artifact attributable to the flat shape and high index of refraction of mammalian sperm heads. This artifact disappears when an epiillumination flow system is used in which the optic axes for illumination and detection and the flow axis are all coincident. Other approaches also eliminate the artifact. The resulting coefficients of variation observed after acriflavine-Feulgen staining are 4-5%, short of the goal of 1.5% required to distinguish between human sperm bearing X and Y chromosomes and to develop a mutagen test system using mice.

Effects of cryopreservation on human sperm deoxyribonucleic acid integrity

2010 ◽  
Vol 93 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Nassira Zribi ◽  
Nozha Feki Chakroun ◽  
Henda El Euch ◽  
Jalel Gargouri ◽  
Ali Bahloul ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Sperm

Human papillomavirus gene sequences in washed human sperm deoxyribonucleic acid

1994 ◽  
Vol 61 (5) ◽  
pp. 982-985 ◽  
Author(s):  
Philip J. Chan ◽  
Brian C. Su ◽  
Theresa Kalugdan ◽  
Ibrahim M. Seraj ◽  
Donald R. Tredway ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Deoxyribonucleic Acid ◽  
Human Sperm ◽  
Gene Sequences

Antioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damage

2018 ◽  
Vol 120 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Şule Ayla ◽  
Gülden Tunalı ◽  
Bülent E. Bilgiç ◽  
Kenan Sofuoğlu ◽  
A.Arman Özdemir ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Caffeic Acid ◽  
Deoxyribonucleic Acid ◽  
Human Sperm ◽  
Caffeic Acid Phenethyl Ester

scholarly journals Detection of Deoxyribonucleic Acid Fragmentation in Human Sperm: Correlation with Fertilization in Vitro1

1997 ◽  
Vol 56 (3) ◽  
pp. 602-607 ◽  
Author(s):  
Jian-Guo Sun ◽  
Andrea Jurisicova ◽  
Robert F. Casper
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Sperm
Sign in / Sign up

Export Citation Format

Share Document