Deoxyribonucleic acid (DNA) methylation and its impact in generation of cancer

2014 ◽  
Vol 3 (3) ◽  
pp. 181
Author(s):  
J Rajeswari ◽  
GP Chakravarthi
Keyword(s):  
Dna Methylation ◽  
Deoxyribonucleic Acid

scholarly journals Influence of maternal age on intracytoplasmic sperm injection outcome and global deoxyribonucleic acid methylation in women undergoing intracytoplasmic sperm injection cycle

2021 ◽  
Vol 10 (6) ◽  
pp. 2185
Author(s):  
Mohammed M. Laqqan ◽  
Maged M. Yassin
Keyword(s):  
Dna Methylation ◽  
Intracytoplasmic Sperm Injection ◽  
Methylation Level ◽  
Maternal Age ◽  
Deoxyribonucleic Acid ◽  
Global Dna Methylation ◽  
Age Group ◽  
Icsi Cycle ◽  
Injection Cycle ◽  
Positive Significant Correlation

Background: Intracytoplasmic sperm injection needs sufficient oocytes of high quality in order to increase the rate of fertilization and pregnancy. This study was designed to investigate the influence of maternal age on the ICSI outcomes in women undergoing to first ICSI cycle and to evaluate the influence of maternal age on global DNA methylation.Methods: A total of 242 females were included in this study with a mean age of 30.5±7.3 years. The participants were divided into three groups depending on women's age≤25, N=70; 26-35, N=102 and>35, N=70). The genomic DNA was isolated from the blood samples, then the global DNA methylation was evaluated using ELISA.Results: A significant reduction has been found in the level of anti-Müllerian hormone (AMH), total number of the collected oocyte, mature oocytes, fertilized oocytes and number of embryos transferred in the older females compared to the younger group (p<0.001). While a significant increase has been found in global DNA methylation level in the older females compared to the younger group (p<0.001). A positive significant correlation has been found between global DNA methylation level and maternal age (p<0.001). In contrast, a negative significant correlation has been shown between AMH level, mature oocytes and maternal age (p<0.001).Conclusions: Maternal age has a significant influence on the number of mature oocytes, number of embryos transferred and global DNA methylation. The pregnancy chance is more in the age group less than 35 years.

scholarly journals CORRELATIONS OF DEOXYRIBONUCLEIC ACID METHYLATION, HISTONE ACETYLATION, AND MICRORNA‑320 WITH SORBITOL DEHYDROGENASE IN DIABETIC RETINOPATHY

2019 ◽  
pp. 475-478
Author(s):  
RAMZI AMIN ◽  
DANY HILMANTO ◽  
ARIEF S. KARTASASMITA ◽  
HIKMAT PERMANA
Keyword(s):  
Dna Methylation ◽  
Diabetic Retinopathy ◽  
Histone Acetylation ◽  
Deoxyribonucleic Acid ◽  
Quantitative Polymerase Chain Reaction ◽  
Vascular Complications ◽  
Sorbitol Dehydrogenase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Strong Negative Correlation

Objective: Prolonged and persistent hyperglycemia in diabetes mellitus (DM) leads to a variety of vascular complications, including the retinal disorder of diabetic retinopathy (DR). The mechanism of fructose formation of sorbitol assisted by sorbitol dehydrogenase (SDH) causing the loss of pericytes in the blood vessel is affected by epigenetic work comprised of deoxyribonucleic acid (DNA) methylation, histone acetylation, and microRNA‑320. This study aimed to determine the correlation of DNA methylation, histone acetylation, and microRNA‑320 with SDH in DR. Methods: This case–control study was conducted at a tertiary general hospital from July 2014 to June 2016. Study subjects were type 2 DM patients with and without DR, over 40 years old, suffered from DM for > 10 years. DNA methylation, histone acetylation, and microRNA‑320 were examined by real‑time quantitative polymerase chain reaction, while SDH level examination was carried out by enzyme‑linked immunosorbent assay. Analyses were performed with independent t‑test, Mann–Whitney, Spearman correlation, and multiple linear regression. Results: With respect to SDH, DNA methylation showed no significant correlation so as histone acetylation, in contrary to microRNA‑320 with a very strong negative correlation (r=−0.968, P < 0.005). Conclusion: MicroRNA‑320 was correlated to SDH in a manner of protective properties against the occurrence of DR. Involvement of DNA methylation and histone acetylation was perceptible in influencing SDH enzyme despite their insignificance if they took place individually.

scholarly journals Alterations in Deoxyribonucleic Acid (DNA) Methylation Patterns of Calca, Timp3, Mmp2, and Igf2r Are Associated With Chronic Cystitis in a Cyclophosphamide-induced Mouse Model

Urology ◽  
2013 ◽  
Vol 82 (1) ◽  
pp. 253.e9-253.e15 ◽  
Author(s):  
In-Seon Choi ◽  
Kevin Yu ◽  
Jayoung Kim ◽  
Erika De Guzman ◽  
Daniel J. Weisenberger ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mouse Model ◽  
Deoxyribonucleic Acid ◽  
Chronic Cystitis ◽  
Methylation Patterns

scholarly journals Deoxyribonucleic acid methylation in the enhancer region of the CDKN2A/2B and CDKN2B-AS1 genes in blood vessels and cells in patients with carotid atherosclerosis

2020 ◽  
Vol 25 (10) ◽  
pp. 4060
Author(s):  
Yu. A. Koroleva ◽  
A. V. Markov ◽  
I. A. Goncharova ◽  
A. A. Sleptsov ◽  
N. P. Babushkina ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Peripheral Blood ◽  
Methylation Level ◽  
Deoxyribonucleic Acid ◽  
Healthy Individuals ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Blood Samples ◽  
Cpg Sites ◽  
Atherosclerotic Lesions

Aim. Comparative analysis of the deoxyribonucleic acid (DNA) methylation level in the enhancer region of the CDKN2A/2B and CDKN2B-AS1 genes (9p21.3 locus) in vessels with/without atherosclerotic lesions, as well as in leukocytes of patients with clinically relevant carotid artery (CA) atherosclerosis and healthy individuals.Material and methods. The group of patients with clinically relevant atherosclerosis included 22 individuals with severe stenosis (>80%) of CA. Samples of atherosclerotic plaques, presenting CA regions, and great saphenous veins, as well as peripheral blood samples (leukocytes) were obtained from patients. The control group consisted of 14 individuals with the mild CA stenosis (£24%) and without hemodynamically relevant changes; peripheral blood samples were obtained from each of them. DNA methylation level was assessed by targeted bisulfite sequencing of amplicons.Results. The tissue-specific methylation of 31 CpG-site in the CDKN2A/2B and CDKN2B-AS1 gene enhancer was established: the vascular tissues significantly differed from the peripheral blood leukocytes. At the same time, there was an increase in the methylation level of both certain CpG sites and whole analyzed CA region affected by atherosclerosis (48,6 [34,8; 62,0]%), compared with intact vessels, both arteries (25,2 [23,1; 41,60]%, p=0,0001) and veins (35,0 [31,6; 40,0]%, p=0,0039). Patients had lower methylation levels in all CpG sites in blood leukocytes compared to blood vessel samples (8,7 [6,1; 9,7]%; p<0,05). At the same time, the level of DNA methylation in the blood leukocytes of atherosclerotic patients does not differ from that in healthy individuals (9,3 [8,3; 13,6]%; p>0,8).Conclusion. In the present study, the relationship between an increase in the DNA methylation in the enhancer of the CDKN2A/2B and CDKN2B-AS1 genes in CA and their atherosclerotic lesions was revealed, as well as the tissue-specific DNA methylation between vessels and peripheral blood leukocytes.

scholarly journals Review; Role of epigenetics in cancer

2020 ◽  
Vol 5 (2) ◽  
pp. 51
Author(s):  
Hafiz Ghulam Muhu-Din Ahmed ◽  
Aziz Ullah ◽  
Abdul Malik ◽  
Babar Islam
Keyword(s):  
Dna Methylation ◽  
Cancer Cells ◽  
Noncoding Rna ◽  
Deoxyribonucleic Acid ◽  
Molecular Pathways ◽  
Epigenetic Alterations ◽  
Epigenetic Control ◽  
Micro Rnas ◽  
Novel Targets

The epigenetic alterations are central to numerous human diseases, counting cancer. Typically, cancer has been seen as a hereditary infection, and it is presently getting to be clear that the onset of cancer is gone before by epigenetic anomalies. Examiners within the quickly growing field of epigenetics have recorded broad genomic reconstructing in cancer cells, counting methylation of deoxyribonucleic acid (DNA), chemical alteration of the histone proteins, and RNA-dependent control. Recognizing that carcinogenesis includes both hereditary and epigenetic alterations have driven to distant better an understanding of the molecular pathways that oversee the advancement of cancer and to changes in diagnosing and foreseeing the result of different sorts of cancer. Thinks about of the mechanism (s) of epigenetic control and its reversibility have brought about within the recognizable proof of novel targets which will be valuable in creating unused methodologies for the avoidance and treatment of cancer. Cancer is the appearance of both hereditary and epigenetic adjustments. In spite of the fact that cancer start and movement is overwhelmingly driven by procured hereditary modifications, it is getting to be clear that microenvironment mediated epigenetic annoyances play critical parts in neoplastic advancement. Epigenetics is characterized as heritable changes in quality expression, movement and expression that happen without change in DNA arrangements but which are adequately capable to control the flow of quality expression. The key forms that are mindful for epigenetic control are DNA methylation, adjustments in chromatin (covalent adjustment of center histones), nucleosome situating (physical modification), and post-transcriptional quality direction by noncoding RNA (micro-RNAs). A number of well characterized epigenetic adjustments are connected to distorted quality capacities and modified designs of quality expression that play basic parts within the patho-biology of cancer

Methylation of Deoxyribonucleic Acid in Regenerating Rat Liver

1973 ◽  
Vol 28 (7-8) ◽  
pp. 463-465
Author(s):  
Dieter Lutz ◽  
Helga Grahn ◽  
Hans Kröger
Keyword(s):  
Dna Methylation ◽  
Dna Synthesis ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Deoxyribonucleic Acid ◽  
Thymidine Incorporation ◽  
Regenerating Rat Liver ◽  
Double Label

In double label pulse experiments DNA methylation was compared to DNA synthesis after partial hepatectomy. 24 hours after the operation the highest 14C-thymidine incorporation rates were found as well as the highest 5-methylcytosine labelling derived from (3H-methyl) -methionine. However, synthesis was much more elevated than DNA methylation. Applying Endoxan DNA methylation is reduced to a significantly higher extent than DNA synthesis. Our results indicate that DNA methylation occurs not only combined with DNA synthesis.

DNA methylation in satellite repeats disorders

2019 ◽  
Vol 63 (6) ◽  
pp. 757-771 ◽  
Author(s):  
Claire Francastel ◽  
Frédérique Magdinier
Keyword(s):  
Dna Methylation ◽  
Human Genome ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Repeats ◽  
Tremendous Progress ◽  
Genes Encoding ◽  
Dna Elements ◽  
Near Future

Abstract Despite the tremendous progress made in recent years in assembling the human genome, tandemly repeated DNA elements remain poorly characterized. These sequences account for the vast majority of methylated sites in the human genome and their methylated state is necessary for this repetitive DNA to function properly and to maintain genome integrity. Furthermore, recent advances highlight the emerging role of these sequences in regulating the functions of the human genome and its variability during evolution, among individuals, or in disease susceptibility. In addition, a number of inherited rare diseases are directly linked to the alteration of some of these repetitive DNA sequences, either through changes in the organization or size of the tandem repeat arrays or through mutations in genes encoding chromatin modifiers involved in the epigenetic regulation of these elements. Although largely overlooked so far in the functional annotation of the human genome, satellite elements play key roles in its architectural and topological organization. This includes functions as boundary elements delimitating functional domains or assembly of repressive nuclear compartments, with local or distal impact on gene expression. Thus, the consideration of satellite repeats organization and their associated epigenetic landmarks, including DNA methylation (DNAme), will become unavoidable in the near future to fully decipher human phenotypes and associated diseases.

15 LONGITUDINAL EVALUATION OF MUCOSAL DNA METHYLATION IN ULCERATIVE COLITIS SHOWS DISEASE-SPECIFIC CHANGES

2020 ◽  
Vol 158 (3) ◽  
pp. S50-S51
Author(s):  
Suresh Venkateswaran ◽  
Varun Kilaru ◽  
Hari Somineni ◽  
Jason Matthews ◽  
Jeffrey Hyams ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Dna Methylation ◽  
Longitudinal Evaluation ◽  
Disease Specific
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