scholarly journals GENETIC DIVERSITY OF MACKEREL SCADS, Decapterus macarellus (Cuvier, 1833) IN THE INDIAN OCEAN

2018 ◽  
Vol 23 (2) ◽  
pp. 89
Author(s):  
Achmad Zamroni ◽  
Suwarso Suwarso
Keyword(s):  
Genetic Diversity ◽  
Indian Ocean ◽  
Restriction Fragment Length Polymorphism ◽  
Genetic Analysis ◽  
Fragment Length Polymorphism ◽  
Fish Stock ◽  
The Indian Ocean ◽  
Over Exploitation ◽  
Stock Information ◽  
Restriction Fragment Length

Mackerel scads (Decapterus macarellus) is a small widely distributed pelagic species in ocean. In 2013, monthly catch and abundance index of mackerel scads increased in western part of Sumatera waters. High exploitation of mackerel scads may lead to decrease stock due to the over exploitation. Stock information is very useful for calculating of the potential fish. Genetic analysis is one of the powerful tools to estimate fish stock quickly. Genetic diversity of mackerel scads in this study was analyzed using RFLP (Restriction Fragment Length Polymorphism) with AfaI, EcoR I, HapII, HinfI and TaqI restriction enzyme. The results showed that the lowest genetic diversity of mackerel scads was Labuan population. Kinship Labuan was also the furtherest stock compared to other populations. It can be concluded that the population of Labuan is derived from a different sub-species. The closest kinship was between Aceh and Sibolga stock.

Genetic diversity amongBradyrhizobiumisolates that effectively nodulate peanut (Arachis hypogaea)

1996 ◽  
Vol 42 (11) ◽  
pp. 1121-1130 ◽  
Author(s):  
Bruce E. Urtz ◽  
Gerald H. Elkan
Keyword(s):  
Genetic Diversity ◽  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Arachis Hypogaea ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Dna Homology ◽  
Symbiotic Gene ◽  
Restriction Fragment Length

Symbiotic gene diversity and other measures of genetic diversity were examined in Bradyrhizobium isolates that form an effective symbiosis with peanut (Arachis hypogaea). Initially, restriction fragment length polymorphism (RFLP) analysis using a nitrogenase (nif) gene probe was performed on 33 isolates along with one Bradyrhizobium elkanii and two Bradyrhizobium japonicum strains. Considerable diversity was observed among the RFLP patterns of many of the isolates, especially those from South America. Some isolates, however, were found to have similar nif and subsequent nod (nodulation) gene RFLP patterns, indicating symbiotic gene relatedness. With some noted exceptions, symbiotic gene relatedness correlated with relatedness based on total DNA homology and ribotyping analyses. Symbiotic gene relatedness also correlated with symbiotic effectiveness. The RFLP and DNA homology analyses indicate that bradyrhizobia effective with peanut are genetically diverse and consist of at least three different species. This diversity, however, was not particularly evident with partial 16S rRNA gene sequencing. Sequences obtained from the isolates were very similar to each other as well as to sequences previously reported for other Bradyrhizobium strains.Key words: Bradyrhizobium, nif, peanut, restriction fragment length polymorphism, 16S rRNA.

Genetic analysis of nuclear DNA restriction fragment patterns

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 693-703 ◽  
Author(s):  
Elizabeth M. Gillet
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Genetic Analysis ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) analysis in the broad sense is the analysis of differences in restriction fragment pattern produced by defined target segments within or between cell compartments, cell types, etc., in a single individual or in different individuals. Thus both molecular hybridization and DNA amplification by two-primer extension using the polymerase chain reaction can define target segments for RFLP analysis. The two techniques are outlined with special consideration of characteristics important for genetic analysis. The mode of inheritance of restriction fragment patterns as a prerequisite for their use as genetic markers in inheritance studies is explained, leading to criticism of common usage. The importance of internal restriction sites for the determination of allelic variation is stressed. It is shown that, if target segments are under the control of a single nuclear diploid restriction fragment locus, then complete reconstruction of all parental target segments requires controlled crosses between individuals of like restriction fragment pattern.Key words: genetic analysis, inheritance, restriction fragment length polymorphism, controlled cross, polymerase chain reaction.

scholarly journals Prevalence and Genetic Diversity of C. Jejuni Isolated from Broilers and their Environment Using flaA-RFLP Typing and MLST Analysis

2020 ◽  
Vol 20 (2) ◽  
pp. 485-501
Author(s):  
Viktorija Lėgaudaitė Lydekaitienė ◽  
E. Kudirkienė
Keyword(s):  
Genetic Diversity ◽  
Restriction Fragment Length Polymorphism ◽  
Campylobacter Jejuni ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Wild Birds ◽  
Mlst Analysis ◽  
Sequence Types ◽  
Restriction Fragment Length ◽  
Rflp Typing

AbstractCampylobacter is highly diverse genetically and also undergoes frequent intraspecific recombination. A major source of campylobacteriosis, which is transmitted to humans is found in poultry. The assessment of the genetic diversity among Campylobacter population is critical to our understanding of the epidemiology. The genetic diversity of Campylobacter jejuni isolates in broilers and their environment were investigated by flaA-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). The study revealed that 92.3% of the examined broiler flocks were contaminated with Campylobacter spp. A total number of 35 different flaA types defined by flaA-RFLP were found in 448 C. jejuni isolates originated from broilers, litter, puddles, zones, anteroom and wild birds. The most dominant flaA type was XXV. MLST defined 20 sequence types (STs) belonging to 10 clonal complexes (CCs). Among all the STs 9 isolates (15%) were consigned to 2 different STs (ST-7413 and ST-4800), which could not be assigned. The most common CCs were ST-21 and ST-179. The ST-21 CC was common in broilers and environment (puddle water and concentric zones) and the ST-179 CC was specific to wild birds, but also was found in puddle water and concentric zones.

scholarly journals First insights into the genetic diversity of Mycobacterium tuberculosis strains in Salvador, Bahia State, Brazil

2011 ◽  
Vol 27 (9) ◽  
pp. 1859-1863
Author(s):  
Aída Cristina do Nascimento Silva ◽  
Lucilaine Ferrazoli ◽  
Vera Simonsen ◽  
Joice Neves Reis ◽  
Susan Martins Pereira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Population Structure ◽  
Restriction Fragment Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Genetic Population ◽  
Recent Transmission ◽  
Single Pattern ◽  
Study Population ◽  
Restriction Fragment Length

This study constitutes a first attempt to describe the genetic population structure of Mycobacterium tuberculosis circulating in Salvador, Bahia State, Brazil. A total of 56 confirmed cases of pulmonary tuberculosis, identified between March and June 2008, were analyzed using restriction fragment length polymorphism (IS6110-RFLP). The study population was characterized by a predominance of males (71.43%) over 30 years of age (68.75%). Forty-one isolates were found to belong to a single pattern (73.2%), while 15 (26.7%) were found in group patterns, forming six clusters. The higher level of diversity observed is much more suggestive of endogenous reactivation than recent transmission.

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