scholarly journals Transient increased immunoglobulin levels in a hyper-IgM syndrome patient with COVID-19 infection

2021 ◽  
Vol 49 (6) ◽  
pp. 63-66
Author(s):  
Molood Safarirad ◽  
Ali Abbaszadeh Ganji ◽  
Farzad Nazari ◽  
Reza Yazdani ◽  
Hassan Abolhassani ◽  
...  
Keyword(s):  
Serum Levels ◽  
Multiple Organ ◽  
Immunoglobulin M ◽  
Polymerase Chain Reaction Test ◽  
Positive Real ◽  
Infected People ◽  
Enveloped Virus ◽  
Monthly Injection ◽  
Chain Reaction Test ◽  
Immunoglobulin Levels

Background: The coronavirus disease 2019 (COVID-19) pandemic has affected millions of people around the world. This zoonotic-enveloped virus is primarily transmitted through inhalation. Infected people are commonly asymptomatic or manifest mild symptoms, including fever, cough, diarrhea, and fatigue. However, it may lead to severe patterns associated with multiple organ failure in individuals with an impaired immune system. Objective: Here we report a 7-year-old girl with hyper-immunoglobulin M (IgM) (HIgM) phenotype, admitted to the hospital emergency department with fever, cough, and pneumonia symptoms because of the COVID-19 infection. Coronavirus infection was confirmed by a positive real-time polymerase chain reaction test. Surprisingly, serum levels of both IgG and IgA of the patient were transiently normalized during the COVID-19 infection when tested prior to the monthly injection of intravenous immunoglobulin. After she recovered from the COVID infection, her immunoglobulin levels returned to the primary stage and she demonstrated HIgM phenotype. Conclusion: Since this transient increase in the levels of immunoglobulins was solely observed during the COVID-19 infection, and no other infectious episodes were diagnosed in the patient, clarifying the exact cause would help to understand in a better manner the implications and specification of humoral immunity in patients with primary antibody deficiencies.

Clinical and Paraclinical Characteristics of 57 Deceased COVID-19 Patients in Tehran, Iran: A Retrospective Observational Study

2021 ◽  
Author(s):  
Niayesh Mohebbi ◽  
Sara Pakzad Karamad ◽  
Keyvan Gohari Moghadam ◽  
Mohamadreza Jafary ◽  
Poorya Rajabi ◽  
...  
Keyword(s):  
Observational Study ◽  
Retrospective Observational Study ◽  
Polymerase Chain Reaction Test ◽  
Ct Scans ◽  
Ground Glass ◽  
Positive Real ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Chain Reaction Test

Background and Aims: In 2019, a novel coronavirus called SARS-COV-2 spread throughout the world, causing a pandemic a few months later. As the virus is not well-known and highly contagious, it is important to observe patients for all possible symptoms and comorbidities. This study aimed to demonstrate some demographic, clinical, and paraclinical features of the deceased patients with COVID-19 in Iran. Materials and Methods: A retrospective observational study was designed to demonstrate the clinical and paraclinical features of deceased patients who had a positive real time-polymerase chain reaction test result for COVID-19. The study included all COVID-19 patients who visited Shariati Hospital, Tehran, Iran, from February 20th until May 20th, 2020. All data were analyzed using IBM SPSS version 21 (Mann-Whitney or Fisher’s test). Results: The patients had a mean age of 63.86 ± 16.428, and most were males (59.65%). Diabetes and hypertension were the most frequent comorbidities. The most common symptoms among the patients were dyspnea (64.91%), weakness (61.40%), fever (60.71%), and having ground-glass opacities in the CT scans (85.96%). There was also a rise in lactate dehydrogenase, serum ferritin, D-dimer, and procalcitonin. Conclusions: Older COVID-19 patients are more likely to get more severe forms of the disease. Most of the deceased COVID-19 patients had ground-glass opacities in their CT scans. Dyspnea, fatigue, and fever were the most frequent symptoms. Hypertension and diabetes were the most important comorbidities. There was almost no significant difference between the two genders regarding clinical and paraclinical symptoms.

scholarly journals Comparison of serological and molecular methods in the diagnosis of cytomegalovirus infections in dialysis patients

2020 ◽  
Vol 73 (1-2) ◽  
pp. 43-48
Author(s):  
Jelena Stojcevic-Maletic ◽  
Katarina Baculov ◽  
Slobodanka Bogdanovic-Vasic ◽  
Borko Milanovic ◽  
Natasa Vucinic ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Immunoglobulin M ◽  
Polymerase Chain Reaction Test ◽  
Positive Finding ◽  
Dialysis Patients ◽  
Chain Reaction ◽  
Post Transplantation ◽  
Polymerase Chain ◽  
Chain Reaction Test

Introduction. Cytomegalovirus is the most common cause of infections in the post-transplantation period. A reliable and timely laboratory diagnosis of cytomegalovirus infection in patients on dialysis and in the post-transplantation period is significant because of the possibility of preventing or mitigating the effects of cytomegalovirus disease. The main objective of this study was to compare serological and molecular polymerase chain reaction methods to determine the presence of cytomegalovirus in the blood of dialysis patients. Material and Methods. The study included 28 dialysis patients, potential renal transplant recipients. All patients were evaluated for the presence of cytomegalovirus in the blood by a quantitative polymerase chain reaction method as well as in the serum for the presence of anti-cytomegalovirus Immunoglobulin G and Immunoglobulin M antibodies. Results. According to the comparative enzyme-linked immunosorbent assay for detecting antibodies in dialysis patients, 96.4% were once exposed to the virus, while 7.1% showed current infection not confirmed by polymerase chain reaction test. No statistically significant association was found between the positive finding of anti-cytomegalovirus Immunoglobulin M antibodies and the findings of the polymerase chain reaction cytomegalovirus method when Chi-square (?2) and Fisher?s correlation tests were conducted (p > 0.05). Conclusion. Due to 7.1% false positives results for the presence of anti-cytomegalovirus Immunoglobulin M antibodies in the serum of immunocompromised dialysis patients, not confirmed by polymerase chain reaction test, serological techniques are not reliable in detecting active cytomegalovirus infection causing positive finding of anti-cytomegalovirus Immunoglobulin M, so confirmation of cytomegalovirus deoxyribonucleic acid by polymerase chain reaction method is required.

scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test

Polymerase Chain Reaction for the Direct Detection of Brucella spp. in Milk and Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 164-167 ◽  
Author(s):  
G. TANTILLO ◽  
A. DI PINTO ◽  
A. VERGARA ◽  
C. BUONAVOGLIA
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Detection ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Sheep Milk ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Quality Of Milk ◽  
Chain Reaction Test

A polymerase chain reaction test was developed to detect Brucella spp. directly in milk and cheese and optimized using primers for the BSCP-31 gene. A total of 46 cheese samples produced with sheep and goats milk were assayed, and Brucella spp. was detected in 46% of them, especially in cheese made from sheep milk. This method is of remarkable epidemiologic interest because it is an indirect test indicating the sanitary quality of milk used in dairy industries. The method showed good sensitivity and specificity. It is faster and less expensive than the conventional bacteriological assays.

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