Development of nutrient medium for riboflavin biosynthesis by Eremothecium ashbyi ascomycetes

The object of the study is the riboflavin producer Eremothecium ashbyi Guilliermond 1935 VKPM F-340, the subject of the study is the regularities of riboflavin biosynthesis by the E. ashbyi F-340 strain under different cultivation conditions. Riboflavin is an important micronutrient that is a precursor of the coenzymes flavin mononucleotide and flavinadine dinucleotide, it is necessary for biochemical reactions in all living cells. Population growth and an increase in human needs for vitamin-fortified food and agricultural products is the reason for an increase in demand for riboflavin preparations. Considering this, it is important and economically beneficial to improve the technology for the production of vitamin B2. An important factor that affects the yield of the product is the nutrient medium. At present, the influence of agricultural waste on the biosynthesis of riboflavin is being actively studied in the world. However, not all of the studied types of raw materials are typical for the agriculture of this or that country. Therefore, in order to determine whether this direction of research is promising, it is important to check the effect on the biosynthetic activity of the riboflavin producer of the most common wastes of the domestic industry. In this work, this is done on the example of Ukraine. In the course of the study, microbiological (surface and deep cultivation of E. ashbyi F-340), physicochemical (determination of the amount of biomass by the gravimetric method, determination of the concentration of riboflavin by the spectrophotometric method) and mathematical methods were used. The proposed media with the addition of agricultural waste, providing a higher yield of riboflavin compared to conventional media. The influence of different types of agricultural waste on the biosynthesis of riboflavin by the producer E. ashbyi F-340 was evaluated. The efficiency of using sunflower cake as a component of the nutrient medium is shown. The optimal sources of carbon for the nutrient medium with oil cake have been determined, which increases the yield of riboflavin. Due to the large amount of sunflower cake obtained in Ukraine, its use for modifying the nutrient medium in order to increase the yield of riboflavin in the future will lead to a decrease in the cost of the target product due to the use of cheap and ecological raw materials.

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Catalytic Activity of Zr4+/ZA on The Isomerization Reaction of α-Pinene and It’s Reusability Test

Indonesian Journal of Physics and Nuclear Applications ◽

10.24246/ijpna.v1i3.157-165 ◽

2016 ◽

Vol 1 (3) ◽

pp. 157

Author(s):

Nico Aditya Wijaya ◽

Nanik Wijayati ◽

Edy Cahyono

Keyword(s):

Natural Zeolite ◽

Raw Materials ◽

Batch Reactor ◽

Gravimetric Method ◽

Isomerization Reaction ◽

X Ray ◽

Bet Method ◽

Main Component

Production of turpentine oil from pine resin until December 2014th was reported to reaching 17.150 tones with the wide of pine forest is 876.992,66 acres. The main component of turpentine oil is α-pinena which isomer products have high value for industrial raw materials. Effects concentration of impregnation and reusability test a best catalyst on α-pinene isomerization reaction using catalysts Zr4+/natural zeolite in which modificated with different Zr4+ concentration were analyzed. Characterization of catalyst included X-ray Fluoroscent for detection of Zr concentration in natural zeolites, Surface Area Analyzer for determine catalyst porosity using BET method, and determination of acidity using vapor ammonia and pyridine base adsorption gravimetric method. Isomerization process used a batch reactor with variation in the concentrations of Zr4+ impregnations 10, 15, and 20 w/w as well as reusability test used best catalyst. The highest conversion of α-pinene used Zr4+/ZA 10% catalyst was 3,89% so as Zr4+/ZA 10% catalyst is best catalyst than other catalysts whereas highest conversion of α-pinene used Zr4+/ZA 10% regeneration catalyst was 4,26%. The product is produced from isomerization reaction of α-pinene used Zr4+/ZA all variations catalysts was camphene and limonene.

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Comparative Characteristics of Sugar Determination by Different Methods in Leaves of Nettle

Drug development & registration ◽

10.33380/2305-2066-2020-9-2-91-97 ◽

2020 ◽

Vol 9 (2) ◽

pp. 91-97

Author(s):

O. V. Trineeva

Keyword(s):

Raw Materials ◽

Physiological Activity ◽

Gravimetric Method ◽

Total Content ◽

Complete Information ◽

Biologically Active ◽

Polysaccharide Complex ◽

Plant Raw Materials ◽

Spectrophotometric Methods

Introduction. According to the literature, nettle leaves contain polysaccharides and mucus as an integral part of the hydrophilic fraction. This group of biologically active substances (BAS) is involved in the manifestation of the physiological activity of the infusion. The traditional gravimetric method for the determination of polysaccharides in medicinal plant raw materials (PRM), pharmaceutical substances of plant origin and medicinal herbal preparations cannot provide a true picture of their content due to the presence of impurities. Therefore, it is advisable to determine the reducing sugars that are most fully extracted into the aqueous phase during the preparation of the infusion using modern physicochemical methods of analysis.Aim. The aim of the work was to determine the amount of polysaccharides and simple sugars in nettle leaves by various physico-chemical methods and their comparative characteristics.Materials and methods. A comparative determination of the sum of polysaccharides and simple sugars in the leaves of nettle by pharmacopoeial methods (picrine, anthrone and orcine) was carried out. Free and bound simple sugars were identified and quantified by thin layer chromatography (TLC). The composition and content of free simple sugars was studied by capillary electrophoresis (CE) in raw materials.Results and discussion. The content of free and bound sugars in raw materials has been established. The largest content of both the sum of polysaccharides and free simple sugars in the polysaccharide complex of nettle leaves, as well as the sum of free reducing simple sugars, according to experimental data, was obtained using the picrine method. The content of pentoses in the studied PRM was four times less, which is consistent with the literature data on the preferential construction of polysaccharides of plant objects from sugars of the hexose class. The resulting total content of the fraction of free sugars in the leaves, determined by the CE method, is an order of magnitude less compared with spectrophotometric methods. The obtained results of quantitative determination of the amount of free and bound simple sugars in the studied PRM are consistent with the determination data by the picrine pharmacopoeial method.Conclusion. In general, complete information, depending on the goals of the analysis, on the composition and quantitative content of simple and related sugars in the polysaccharide complex of plant objects can only be obtained by combining spectral methods with CE or TLC.

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PENGARUH PENAMBAHAN BIOCHAR TERHADAP PENURUNAN KADAR TOTAL PETROLEUM HYDROCARBON (TPH) PADA TANAH TERCEMAR MINYAK BUMI

Jurnal Tanah dan Sumberdaya Lahan ◽

10.21776/ub.jtsl.2021.008.2.12 ◽

2021 ◽

Vol 8 (2) ◽

pp. 407-414

Author(s):

Aryo Sasmita ◽

Amalia Syakinah ◽

Ulfatun Nisa

Keyword(s):

Petroleum Hydrocarbon ◽

Raw Materials ◽

Agricultural Waste ◽

Petroleum Industry ◽

Gravimetric Method ◽

Total Petroleum Hydrocarbon ◽

Hydrocarbon Contamination ◽

Empty Shell ◽

Petroleum Contaminated Soil ◽

And Control

Hydrocarbons are compounds produced as a result of the activities of the petroleum industry, which can pollute the soil and waters. Due to the amount of waste, biochar from agricultural waste could potentially be used as a soil amendment agent for hydrocarbon contamination. The high lignocellulose in oil palm shells and empty bunches makes them potential raw materials for biochar. The purpose of this study was to explore the effect of biochar application on petroleum-contaminated soil to reduce Total Petroleum Hydrocarbon (TPH) levels. In this study, the dosage of biochar was added to the soil contaminated with petroleum with a variation of 3%, 5%, and 7% (w/w) and control without the addition of biochar. The parameters analyzed were TPH levels by gravimetric method every week for four weeks. The results showed that the addition of biochar had an effect on the degradation of TPH. The greater the dose of biochar used, the higher the percentage of TPH degradation. The highest decrease in TPH levels occurred at the addition of biochar dose by 7%, where the empty shell was 60.65%, and empty bunches was 54.1% which was greater than without the addition of biochar by 32.79%.

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