scholarly journals In vitro evaluation of fungicide and biocontrol agents against Alternaria helianthi causing leaf blight of sunflower (Helianthus annuus L.)

2017 ◽  
Vol 10 (2) ◽  
pp. 333-338
Author(s):  
K. ATHIRA
Keyword(s):  
Helianthus Annuus ◽  
Leaf Blight ◽  
Biocontrol Agents ◽  
In Vitro Evaluation ◽  
Helianthus Annuus L ◽  
Alternaria Helianthi

scholarly journals In vitro and in vivo water stress in sunflower, Helianthus annuus L

Helia ◽  
2004 ◽  
Vol 27 (40) ◽  
pp. 227-236 ◽  
Author(s):  
H. Turhan ◽  
I. Baser
Keyword(s):  
Water Stress ◽  
Helianthus Annuus ◽  
Helianthus Annuus L

scholarly journals Inhibition of Heme Polymerization Invitro Assay Of Extract of Sirih Leaf (Piper betle Linn.) and Sun Flower Leaves (Helianthus annuus L.)

2020 ◽  
Vol 7 (1) ◽  
pp. 22 ◽  
Author(s):  
Ami Tjitraresmi ◽  
Moelyono Moektiwardoyo ◽  
Yasmiwar Susilawati
Keyword(s):  
Helianthus Annuus ◽  
Antimalarial Activity ◽  
Antimalarial Drugs ◽  
Piper Betle ◽  
Phytochemical Screening ◽  
Helianthus Annuus L ◽  
Betel Leaf ◽  
The People ◽  
Ic50 Values

Malaria is a disease that occurs in tropical countries like Indonesia. The incidence of malaria in the world is still quite high and the occurrence of cases of Plasmodium resistance to antimalarial drugs and the widespread of resistance have prompted researchers to look for new antimalarial drugs, especially from natural materials. Betel leaf (Piper betle Linn.) And sunflower leaf (Helianthus annuus L.) have long been used by the people of Indonesia as an antimalarial drug. The purpose of this study was to determine antimalarial activity through inhibition of heme polymerization and determine secondary metabolite compounds by phytochemical screening from betel leaves and sunflower leaves. The heme polymerization inhibition activity assay was carried out by the in-vitro method using a microplate reader at 415 nm and 630 nm wavelengths. IC50 values of betel leaf extract and sunflower leaf were 178.67 μg/ml and 160.10 μg/ml, respectively. Phytochemical screening results from betel leaf showed the presence of flavonoids, polyphenols, tannins, quinones, saponins, and monoterpenoids-sesquiterpenoids, while sunflower leaves contain alkaloids, polyphenols, flavonoids, steroids and monoterpenoids-sesquiterpenoids.Keywords: Piper betle Linn., Helianthus annuus L., Malaria, Heme Polymerization

scholarly journals Prevalence and Severity of Leaf Blight Disease of Chrysanthemum in Southern regions of Karnataka and in-vitro Fungicidal Effect on Alternaria alternata

2015 ◽  
Vol 9 (3) ◽  
pp. 38-45 ◽  
Author(s):  
G. Shamala ◽  
G. R. Janardhana
Keyword(s):  
Alternaria Alternata ◽  
Disease Incidence ◽  
Life Sciences ◽  
Leaf Blight ◽  
Cut Flowers ◽  
In Vitro Evaluation ◽  
Karnataka State ◽  
Commercially Important ◽  
Blight Disease

Chrysanthemum is one of the commercially important flower crop with great potential both as loose and cut flowers in Karnataka state. In the present study, prevalence and incidence of leaf blight disease was studied in six major growing districts of South Karnataka during 2012 - 2013. The pathogen associated with leaf blight disease was isolated and identified as Alternaria alternata. The study revealed that, the disease was distributed in Southern districts of Karnataka wherever Chrysanthemum is grown. The varieties Chandini and Karnel were more susceptible to blight showing highest disease incidence (100%) and severity (100%) recorded from Mandya and Mysore regions. The lowest disease incidence, severity and PDI of 18.40%, 38.68% and 24.89% were recorded on Rajawhite variety in Bangalore region. During 2013, the highest disease incidence, severity and PDI with 100%, 98.47%, and 45.71% were recorded in Mysore district on Chandini variety. In vitro evaluation of four fungicides on Alternaria alternata revealed that Carbendazim+Mancozeb was most effective with 95.65% inhibition followed by Carbendazim, Mancozeb and Metalaxyl+Mancozeb with 68.26%, 91.30% and 91.30% inhibition of the mycelium at 2.0% concentration.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12465   International Journal of Life Sciences 9 (3): 2015; 38-45

scholarly journals INHIBITION OF α-AMYLASE AND α-GLUCOSIDASE BY (6RS)-22-HYDROXY-23,24,25,26,27-PENTANOR-VITAMIN-D3-6,19-SULFUR DIOXIDE-ADDUCT, MANOALIDE AND 5β-CHOLESTANE-3α,7α,12α,24,25,26-HEXOL ISOLATED FROM ACETONE EXTRACT OF HELIANTHUS ANNUUS L. SEEDS

2018 ◽  
Vol 10 (5) ◽  
pp. 39 ◽  
Author(s):  
Varsha V. Sonkamble ◽  
Nilesh S. Wagh ◽  
Laxmikant H. Kamble
Keyword(s):  
Helianthus Annuus ◽  
Vitamin D3 ◽  
In Silico ◽  
Binding Energies ◽  
Acetone Extract ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Helianthus Annuus L ◽  
Kinetics Studies

Objective: This investigation includes characterization of phytochemicals from acetone extract of Helianthus annuus L. seeds responsible for α-amylase and α-glucosidase inhibition revealed from in vitro and in silico approaches.Methods: Seed extract was qualitatively and quantitatively analysed for the presence of bioactive molecules. In vitro α-amylase and α-glucosidase inhibition assays and kinetics studies for α-glucosidase were done. Thin layer chromatography (TLC) autography of extract was done to screen potent inhibitors and characterized by high-resolution liquid chromatography-mass spectrometry (HR LC-MS). Characterized molecules were further used for in silico studies.Results: Qualitative investigation reveals the presence of flavonoids, glycosides, alkaloids, terpenoids, and steroids. Quantitative analysis for total phenolic content and total flavonoid content of the extract was 0.1±0.005 mg/ml GAE and 0.025±0.003 mg/ml QE respectively. Percent inhibition of α-amylase and α-glucosidase ascertained in presence of extract was 60.42±0.6 and 83.22±0.18 at 0.01 mg while 36.24±0.81 and 37.67±0.15 at 0.005 mg of extracts for both enzymes respectively. Kinetics studies of α-glucosidase inhibition illustrated the non-competitive type of inhibition. TLC autography inhibition patterns were characterized by HR LC-MS. Characterized molecules on docking revealed (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol as the best docked molecules with lowest binding energies of-12.5,-11 and-10.2 kcal/mol for α-amylase and-14.2,-11 and-11.2 kcal/mol for α-glucosidase respectively.Conclusion: Results clearly suggested that (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol could be considered as lead molecules for the discovery of potent antidiabetic agents. 

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