scholarly journals A new approach for open surgery on the abdominal aorta and iliac blood vessels – Piljic Method

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Dragan Piljic ◽  
Dilista Piljic
2003 ◽  
Vol 285 (6) ◽  
pp. H2797-H2803 ◽  
Author(s):  
Yingbi Zhou ◽  
Wessel P. Dirksen ◽  
Gopal J. Babu ◽  
Muthu Periasamy

Genetically altered mice are increasingly used as experimental models. However, ANG II responses in mouse blood vessels have not been well defined. Therefore, the aim of this study was to determine the role of ANG II in regulating major blood vessels in C57/BL6J mice with isometric force measurements. Our results showed that in mouse abdominal aorta ANG II induced a concentration-dependent contraction (EC50 4.6 nM) with a maximum contraction of 75.1 ± 4.9% at 100 nM compared with that of 60 mM K+. Similarly, femoral artery also exhibited a contractile response of 76.0 ± 3.4% to the maximum concentration of ANG II (100 nM). In contrast, ANG II (100 nM)-induced contraction was significantly less in carotid artery (24.5 ± 6.6%) and only minimal (3.5 ± 0.31%) in thoracic aorta. The nitric oxide synthase inhibitor Nω-nitro-l-arginine methyl ester and the AT2 antagonist PD-123319 failed to enhance ANG II-induced contractions. However, an AT1 antagonist, losartan (10 μM), completely inhibited ANG II (100 nM) response in abdominal aorta and carotid artery. An AT1 agonist, [Sar1]-ANG II (100 nM), behaved similarly to ANG II (100 nM) in abdominal aorta and carotid artery. RT-PCR analyses showed that mouse thoracic aorta has a significantly lower AT1 mRNA level than abdominal aorta. These results demonstrate that major mouse vessels exhibit differential contractions to ANG II, possibly because of varied AT1 receptor levels.


2017 ◽  
Vol 38 ◽  
pp. e16-e17 ◽  
Author(s):  
Yamume Tshomba ◽  
Marco Leopardi ◽  
Ciro Ferrer ◽  
Piergiorgio Cao ◽  
Paola De Rango ◽  
...  

Author(s):  
Corazzi Francesco ◽  
Brusa Stefania ◽  
Giustiniano Enrico ◽  
Civilini Efrem ◽  
Poletto Giorgio Luca ◽  
...  

Cephalalgia ◽  
2009 ◽  
Vol 30 (1) ◽  
pp. 27-36 ◽  
Author(s):  
S Gupta ◽  
DK Bhatt ◽  
LJ Boni ◽  
J Olesen

Intravital microscopy on a closed cranial window allows one to measure change in the diameter of cranial blood vessels after intravenous (i.v.) administration of pharmacodynamic substances. Putative targets being pursued in migraine are large vasodilating peptide molecules such as calcitonin gene-related peptide (CGRP) and pituitary adenylate cyclase polypeptide (PACAP)-38. High i.v. doses are required to study their craniovascular pharmacology. Unfortunately, this leads to a drop in blood pressure (BP) that subsequently causes blood vessels to dilate by autoregulation. Hence it is difficult to decipher what effect is caused by direct receptor agonist interaction or contributed by autoregulation. In the present study we infused substances with an ingenious indwelling catheter in the common carotid artery in rats. Intracarotidly seven-, 12- and 17-fold lower doses of CGRP, PACAP-38 and capsaicin were required, respectively, compared with i.v. infusion to induce the same dilation in dural artery. Dilating intracarotid (i.c.) doses caused no or a minimal fall in BP, whereas equi-responsive i.v. doses caused a marked BP reduction. The CGRP blocking potential of olcegepant was amplified by > 20 times on i.c. infusion. Pial artery responses to CGRP did not change with i.c. infusion, demonstrating that dilations after i.v. CGRP are mediated by autoregulation rather than through specific receptors. We applied CGRP topically, which induced concentration-dependent dural vasodilation, but no effect on pial artery or on BP. In conclusion, this new approach offers an improvement of the existing model by allowing more accurate assessment of effects of pharmaca on the cranial vasculature without inducing significant systemic effects.


2015 ◽  
Vol 69 (1-2) ◽  
pp. 31-40
Author(s):  
Milos Blagojevic ◽  
Dusko Vitorovic ◽  
Ivana Adamovic ◽  
Ivana Nesic ◽  
Zlata Brkic ◽  
...  

Ground squirrel is the only representative of its genus in our country. As experimental animal is used in microbiology, parasitology, immunology and pharmacology. The aim of this study was to examine a part of ground squirrel cardiovascular system and thus help better understanding of anatomy of the body of this specific animal as well as to contribute to comparative anatomy. The studies were perfomed on six ground squirrels, both sexes, weight between 200- 300 g. In order to obtain the arterial vascularization of the kidney, contrast mass gelatin stained with painting tempera was injected into the abdominal aorta after bleeding out. After the injection, blood vessels were prepared and photographed. Corosive preparations of the vein blood vessels of the kidneys were obtained by injection of Byocril into the right azygos vein after bleeding out. After injection, the preparations were placed into 5% NaOH for 96 hours or 10% NaOH for 48 hours. After that the preparations were rinsed with hot water and photographed. A. renalis dextra arises from the lateral wall of the abdominal aorta, 3-4 mm caudal to A. mesenterica cranialis. In most cases, this vessel divides into two or three branches before entering the hilus of the right kidney. A. renalis sinistra arises from the lateral wall of the abdominal aorta, 7-9 mm caudal to the right renal artery. Often, instead of one left renal artery, there are two, rarely three. Based on the results of our study, we concluded that in ground squirrel there is one A. renalis dextra and often two, rarely three Aa. renales sinistrae. In renal venous vascularization, both right and left renal vein are involved. Before entering the kidney, both of them divide into cranial and caudal branch, undergoing renal hilus, enter the renal sinus and continues to branch out into smaller branches.


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