Pancreas transplantation in the treatment of diabetes mellitus type 1: modern aspects

Diabetes mellitus is a significant social problem. In the Russian Federation, the prevalence of diabetes type 1 is 340.000 people, 21% of them having diabetic nephropathy, as well as other secondary complications leading to disability and high mortality. There are several options for diabetic patients with chronic kidney disease dialysis: kidney transplantation with insulin therapy, simultaneous kidney-pancreas transplant or islet transplant. Good long-term results could be obtained by the whole pancreas and kidney combined transplantation through maximum metabolism recovery comparing to other replacement therapy options. The recipientselection with special attention to the cardiovascular system evaluation is essential to successful transplantation. The proper pancreatic graft assessment needs a wide range of criteria to be analyzed. Integral scores can simplify and systematize the assessment. The choice of preservation technique is important to pancreatic graft ischemia-reperfusion injury minimization. Despite the progress in graft preservation methods, cold storage using University of Wisconsin (UW) solution remains to be the gold standard. The evolution of surgical technique has resulted in utilizing the most physiologic variations of exocrine drainage and venous outflow of the pancreas, so-called portoenteric drainages, which were designed to improve remote metabolic effects and to reduce complications probability.

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Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-αInhibitors

Mediators of Inflammation ◽

10.1155/2014/946209 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Jolanta Myśliwska ◽

Monika Ryba-Stanisławowska ◽

Marcin Smardzewski ◽

Bartosz Słomiński ◽

Małgorzata Myśliwiec ◽

...

Keyword(s):

Diabetes Mellitus ◽

Microvascular Complications ◽

Diabetes Mellitus Type 1 ◽

Diabetes Mellitus Type ◽

Diabetic Patients ◽

Monocyte Subsets ◽

Juvenile Onset ◽

Monocyte Apoptosis ◽

Juvenile Onset Diabetes

Diabetes mellitus type 1 is associated with an enhanced apoptosis of different cells and tissues, accelerating occurrence of diabetic microvascular complications. The aim of our study was to determine spontaneous apoptotic potential of the monocyte subsets in juvenile-onset complication-free diabetes mellitus type 1 and to compare them with the corresponding values of the healthy. Moreover, we wanted to assess effects of TNF-R1 blocking agents and those of general TNF-αblocker (Infliximab) on spontaneous apoptosis of monocytes. Sixty randomly selected DM1 patients (14.5 ± 3.2 years) and 30 healthy (13.5 ± 2.8 years) volunteers were enrolled in the study. Our results indicate that three monocyte subsets are distinguishable in the groups of young diabetic patients and the healthy, similarly to in the blood of adults. DM1 patients were characterized by higher values of apoptotic monocytes than the healthy. The manipulation with drugs inhibiting TNF-R1 expression diminished the pool of CD16+apoptotic monocytes. Infliximab reduced the apoptotic CD16−cells. In conclusion, diabetes mellitus type 1 is associated with greater apoptosis of three monocyte subsets which may contribute to the development of microvascular complications. TNF-αmodifiers appear to ameliorate monocyte apoptosis. They may be useful for controlling excessive monocyte apoptosis in diabetic patients.

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Peculiarities of Physical Activity and Self‑Esteem of Young People with Diabetes Mellitus Type 1 and Healthy Persons Aged 18–25 Years

Baltic Journal of Sport and Health Sciences ◽

10.33607/bjshs.v4i83.305 ◽

2018 ◽

Vol 4 (83) ◽

Author(s):

Sandrija Čapkauskienė ◽

Daiva Vizbaraitė ◽

Deimantė Šeštokaitė

Keyword(s):

Physical Activity ◽

Diabetes Mellitus ◽

Healthy Subjects ◽

Self Esteem ◽

Diabetes Mellitus Type 1 ◽

Diabetes Mellitus Type ◽

Diabetic Patients ◽

Patients With Diabetes ◽

Healthy Persons

Research background and hypothesis. Careful diabetes control slows the onset and progression of life-threatening complications, the development of disability and early disability-related unemployment, and prolongs life expectancy (Danytė et al., 2000). The benefits of physical activity on regular basis comprise improved cardiovascular health, increased lean body mass, improved blood lipid profile, enhanced psycho-social wellbeing and decreased obesity (Riddell, Iscoe, 2006). Physical activity is one of the main factors influencing glucose level in diabetic patients’ blood (Wiśniewski, 2010). Analysis of self-esteem of the studied revealed a wide range of findings, from trying to outline the modest achievements, pride, and even unwillingness to discuss it to low self-esteem, feeling of guilt and self-reproach for mistakes and failures (Žemaitis, 1995). The aim of the study was to determine physical activity and self-esteem of healthy subjects and patients with diabetes mellitus type 1 aged 18–25 years.Methods. The study included 140 individuals (aged from 18 to 25 years). Among 41 patients with type 1 diabetes mellitus there were 33 young women and 8 young men, and among 99 healthy persons – 79 young women and 29 young men. All the subjects were asked to fill in the questionnaire orientated to physical activity and self-esteem. The short IPAQ questionnaire was used to research physical activity and Rosenberg’s Self-Esteem Scale was used to assess self esteem.Research results. Approximately 60% of subjects with diabetes mellitus type 1 and about 50% of healthy persons rated their physical activity as moderate. Intensive 60-minute-physical activity was reported by 48.5% of healthy subjects and 34.1% of diabetic patients, moderate 60-minute-physical activity was pointed out by 38.2% of diabetic patients and 35.8% of healthy research participants. The largest walking interval was 1–1.5 hours: in the diabetic group– 28.8%, in the healthy group – 31.65%. Healthy young men and women were physically more active than diabetic patients. Self-esteem in both genders of healthy subjects and diabetic patients was determined as moderate.Discussion and conclusions. Physical activity of women and men with diabetes mellitus type 1 aged 18–25 years was valued as moderate, meanwhile physical activity in healthy persons – moderate or high. Self-esteem is moderate in both groups of patients with diabetes and healthy persons. Healthy men are more active than diabetic patients, similarly, women having diabetes mellitus type 1 are more physically passive than healthy ones. Both patients with diabetes mellitus type 1 and healthy individuals aged 18–25 reported moderate self-esteem.Keywords: diabetes mellitus type 1, physical activity, self-esteem.

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Effect of Thrombin Fragment (TP508) on Myocardial Ischemia Reperfusion Injury in a Model of Type 1 Diabetes Mellitus

Circulation ◽

10.1161/circulationaha.109.928374 ◽

2010 ◽

Vol 122 (11_suppl_1) ◽

pp. S162-S169 ◽

Cited By ~ 9

Author(s):

L. M. Chu ◽

R. M. Osipov ◽

M. P. Robich ◽

J. Feng ◽

M. R. Sheller ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Myocardial Ischemia ◽

Type 1 Diabetes Mellitus ◽

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Myocardial Ischemia Reperfusion Injury ◽

Myocardial Ischemia Reperfusion

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Novel Flow Cytometric Immunoassay for Detection of Proinsulin Autoantibodies in Diabetes Mellitus Employing a Recombinant Autoantigen Expressed in E. coli

Frontiers in Immunology ◽

10.3389/fimmu.2021.648021 ◽

2021 ◽

Vol 12 ◽

Author(s):

Adriana Victoria Sabljic ◽

Silvina Sonia Bombicino ◽

Juan Ignacio Marfía ◽

Luciano Lucas Guerra ◽

Alberto Penas Steinhardt ◽

...

Keyword(s):

Diabetes Mellitus ◽

Dynamic Range ◽

Geometric Mean ◽

Reference Method ◽

Diabetic Patients ◽

Analytical Sensitivity ◽

E Coli ◽

Flow Cytometric ◽

Wide Range

IntroductionInsulin and proinsulin autoantibodies (IAA/PAA) are usually the first markers to appear in patients with type 1 Diabetes Mellitus (T1DM) and their prevalence ranges from 10 to 60% in the child-adolescent population. The reference method for IAA/PAA detection is the Radioligand Binding Assay (RBA), a highly specific and sensitive technique, but expensive and polluting. The aim of this work was to develop a novel flow cytometric microsphere-based immunoassay (FloCMIA) for PAA detection, employing recombinant human proinsulin (PI), as an alternative method to RBA, less expensive and harmful to the environment.Materials and MethodsHuman PI was expressed as Thioredoxin fusion protein (TrxPI) in E. coli and a fraction was biotinylated. A double paratope model was used in which samples were incubated with TrxPI–biotin and microspheres adsorbed with TrxPI. The immune complexes were revealed using Streptavidin–Phycoerythrin. The geometric mean of the signals was analyzed, and the results were expressed as Standard Deviation scores (SDs). Sera from 100 normal human control and from 111 type 1 diabetic patients were evaluated by FloCMIA. To correlate the novel assay with RBA, 51 diabetic patients were selected, spanning a wide range of PAA reactivity by RBA.ResultsThe study of ROC curves allowed choosing a cut-off value of 3.0 SDs and the AUC was 0.705, indicating that FloCMIA has fair ability to distinguish between samples from each group. A prevalence of 50% for PAA was obtained in the population of diabetic patients studied. The specificity was 96% and the analytical sensitivity (percentage of patients RBA positive, also positive by FloCMIA) was 69%. There was a substantial agreement between methods (kappa statistic=0.700).ConclusionsA novel immunoassay based on flow cytometry that uses easy-to produce recombinant PI was developed. This assay constitutes an innovative and cost-effective alternative to RBA for the determination of PAA in patients’ sera. The method developed here, presents good performance and a wide dynamic range together with a small required sample volume. Furthermore, these results make it possible to develop multiplex immunoassays that allow the combined detection of autoantibodies present in T1DM and other related autoimmune diseases.

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Functional hearing examinations in patients suffering from diabetes mellitus type 1 in regard to disease duration

Medicinski pregled ◽

10.2298/mpns1006318p ◽

2010 ◽

Vol 63 (5-6) ◽

pp. 318-323

Author(s):

Goran Pudar ◽

Ljiljana Vlaski ◽

Danka Filipovic ◽

Ilija Tanackov

Keyword(s):

Diabetes Mellitus ◽

Hearing Loss ◽

Evoked Potentials ◽

Sensorineural Hearing Loss ◽

Disease Duration ◽

Diabetes Mellitus Type 1 ◽

Sensorineural Hearing ◽

Diabetes Mellitus Type ◽

Diabetic Patients

Introduction. Problems of hearing disturbances in persons suffering from diabetes have been attracting great attention for many decades. Material and methods. In this study we examined the auditory function of 50 patients suffering from diabetes mellitus type 1 of different duration by analyzing results of pure-tone audiometry and brainstem audi?tory evoked potentials. The obtained results of measuring were compared to 30 healthy subjects from the corresponding age and gender group. The group of diabetic patients was divided according to the disease duration (I group 0-5 years; II group 6-10 years, III group over 10 years). Results and discussion. A statistically significant increase of sensorineural hearing loss was found in the diabetics according to the duration of their disease (I group = 14.09%, II group = 21.39%, III group = 104.89%). The results of the brain stem auditory evoked potentials, the significance threshold being p=0.05 between the controls and the diabetics at all levels of absolute latency of right and left sides, did not show significant differences in the mean values. In the case of interwave latencies, the diabetic patients were found to have a significant qualitative difference of intervals I-III and I-V on both ears in the sense of internal distribution of response. In cases of sensorineural hearing loss we found a significant connection with prolonged latencies of I wave on the right ear and of I and V waves on the left ear. In all probability, the cause of these results could be found in distinctive individuality of the organism reactions to the consequences of this disease (disturbance in the distal part of n. cochlearis). Conclusion. The results of research have shown the existence of a significant sensorineural hearing loss in the patients with diabetes mellitus type 1 in accordance to the disease duration. We also found qualitative changes of brainstem auditory evoked potentials in the diabetic patients in comparison to the controls as well as significant quantitative changes in regard to the presence of sensorineural hearing loss of the patients.

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Pemafibrate suppresses oxidative stress and apoptosis under cardiomyocyte ischemia‑reperfusion injury in type 1 diabetes mellitus

Experimental and Therapeutic Medicine ◽

10.3892/etm.2021.9762 ◽

2021 ◽

Vol 21 (4) ◽

Author(s):

Wei Li ◽

Jianxin Xu ◽

Xin Guo ◽

Xinhua Xia ◽

Yanling Sun

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion

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Inhibition of 2C Coxsackie B Virus Protein to Decrease Pathogenicity of Diabetes Mellitus Type 1

Current Computer - Aided Drug Design ◽

10.2174/1573409915666190820154422 ◽

2020 ◽

Vol 16 (3) ◽

pp. 318-326

Author(s):

Amina Amin ◽

Muhammad A. Rasheed ◽

Rana A. Diwan ◽

Muhammad Shahid ◽

Saddia Bano ◽

...

Keyword(s):

Diabetes Mellitus ◽

Environmental Factors ◽

Small Rna ◽

Rna Virus ◽

Diabetes Mellitus Type 1 ◽

Diabetes Mellitus Type ◽

Diabetic Patients ◽

Lead Compounds ◽

Coxsackie B4 Virus

Background: Insulin-dependent Diabetes Mellitus Type 1 (T1D) also referred to as autoimmune diabetes. T1D is a chronic disease which is characterized by way of insulin deficiency. The deficiency is due to the loss of pancreatic β cells and leads to hyperglycemia. There are many factors which play a significant role in T1D disease pathogenicity including genetic predisposition, the immune system, and environmental factors. The environmental factors may include Coxsackie B4 virus, a small RNA virus. Objective: The objective of current in silico study is to identify active lead compounds against Coxsackie B4 virus, a small RNA virus which has been reported in diabetic patients after PCR. There is a need to predict inhibitors against TID caused by Coxsackie B4 viral protein that may be used as a drug against TID in the future. Methods: For this purpose, different bioinformatics databases and tools were used. The protein structure generation and validation, retrieval of ligands and their properties analysis were performed by different databases, web servers, and software tools. Moreover, the docking tools were used to identify the target site of the protein and interaction of different inhibitors with the target protein molecule. Results: Based on the analysis, two lead compounds ZINC00034488 and ZINC00034585 were selected as potential drugs. These compounds are non-toxic and have best interaction energy and fulfill Lipinski rule, Veber rule, Ghose Rule, Weighted QED, Unweighted QED and BBB likeness parameters. Conclusion: Our work will help researchers to get an idea about the understanding of chemicals against Coxsackie B4 Viruses and helpful to design a drug and test these chemicals to overcome Diabetes Mellitus Type 1 caused by Coxsackie B4 virus.

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Remote Liver Ischemic Preconditioning Attenuates Myocardial Ischemia/reperfusion Injury in Streptozotocin-induced Diabetic Rats

10.21203/rs.3.rs-38824/v1 ◽

2020 ◽

Author(s):

Xinhao Liu ◽

Hui Chen ◽

Zhibing Yan ◽

Lei Du ◽

Dou Huang ◽

...

Keyword(s):

Diabetes Mellitus ◽

Myocardial Ischemia ◽

Infarct Size ◽

Ischemic Preconditioning ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Diabetic Rats ◽

Protective Effects ◽

Myocardial Ischemia Reperfusion

Abstract BACKGROUND: Diabetes mellitus (DM) exhibits a higher sensitivity to myocardial ischemia/reperfusion(I/R)injury and may compromise the effectiveness of cardioprotective interventions, including ischemic preconditioning. We previously found that liver ischemic preconditioning(RLIPC) could limit infarct size post I/R in normal rat hearts and further exerted anti-arrhythmic effects in diabetic or non-diabetic rats after myocardial I/R, however, little is known regarding the effect of RLIPC on infarct-sparing in diabetic hearts. In this study, we evaluated the protective effects of RLIPC on I/R injury in streptozotocin (STZ)-induced type 1 diabetic rats.METHODS:Type 1 diabetes mellitus was induced by one-time intraperitoneal injection of streptozotocin in Sprague–Dawley rats. Rats were exposed to 45 min of left anterior descendin(LAD) coronary artery occlusion, followed by 3 h of reperfusion. For liver ischemic preconditioning, four cycles of 5 min of liver I/R stimuli were performed before LAD occlusion. the cardioprotective effect of RLIPC was determined in diabetic rats.RESULTS: Compared to non-RLIPC treated DM rats, RLIPC treatment significantly reduced infarct size in diabetic hearts post I/R. RLIPC also improved cardiac functions including LVESP, LVEDP, dp/dtmax, and -dp/dtmax. In addition, RLIPC could largely preserved cardiac morphology by reducing the pathological score post I/R in diabetic hearts. Finally, western blotting analysis showed that RLIPC stimulated phosphorylation of ventricular GSK-3β and STAT-5, which are key components of RISK and SAFE signaling pathways.

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