scholarly journals EVALUATION OF AN INTERRELATION OF TARGET BLOOD PRESSURE ACHIEVEMENT AND COMPLICATIONS AND OUTCOMES OF PREGNANCY IN ARTERIAL HYPERTENSION

2014 ◽  
Vol 13 (6) ◽  
pp. 23-27 ◽  
Author(s):  
V. S. Chulkov ◽  
N. K. Vereina ◽  
S. P. Sinitsyn ◽  
V. F. Dolgushina
Keyword(s):  
Arterial Hypertension ◽  
Pregnant Women ◽  
Clinical Course ◽  
Renin Angiotensin System ◽  
Standard Laboratory ◽  
Chain Reaction ◽  
Genetic Investigation ◽  
Angiotensin System ◽  
Lower Weight ◽  
Polymerase Chain

Aim. To evaluate the influence of target BP achievement on clinical course and outcomes of pregnancy in pregnant women with arterial hypertension (AH).Material and methods. A cohort study with prospective cohort. Totally 300 pregnant women included; of those in to the 1st group 103 included with AH, who had not reached target BP; into the 2nd – 97 women with AH, who had reached target BP; control consisted of 100 women without AH. Women underwent clinical examination and observation, analysis of medical data, standard laboratory and instrumental investigation, genetic investigation by polymerase chain reaction.Results. Women who had not reached target BP pregnancy complicated more often with fetus development retardation and pre-eclampsy, and in pregancy outcomes there were preterm delivery and antenatal fetus death, newborns from this group had lower weight and height; also they had more often mutation of D-allele of ACE gene (I/D) and mutant C-allele of receptor 1 type angiotensine II gene (ATR11166 A/C) comparing to the group with achieved BP and with controls. By the result of logistic regression study the factor independently associated with total worse outcome, were premature deliveries in anamnesis - increase the risk almost 6 times (OR=5,93, 95% CI 1,83-19,2; p=0,003), pre-eclampsy during current preganancy – increases risk 3,7 times (OR=3,68, 95% CI 1,48-9,16; p=0,005), and target BP acievement (less than 140/90 mmHg) decreases the risk of total negative outcome 8 times (OR=0,12, 95% CI 0,05-0,28; p<0,001).Conclusion. Target BP achievement in pregnant women with AH might be an independent factor influencing the prevalence of obstetric complications and negative outcomes of pregnancy. A definite impact on target BP levels achievement make genetic polymorphisms of renin-angiotensin system genes.

scholarly journals Peculiarities of the clinical picture of arterial hypertension depending on polymorphic variant of AGTR1 gene

2017 ◽  
Vol 95 (5) ◽  
pp. 404-407
Author(s):  
T. Yu. Zotova ◽  
A. P. Kubanova ◽  
M. M. Azova ◽  
Aissa Amira Aut
Keyword(s):  
Blood Pressure ◽  
Metabolic Syndrome ◽  
Arterial Hypertension ◽  
Clinical Course ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Night Time ◽  
Angiotensin System ◽  
Polymerase Chain ◽  
Body Weight Index

According to the literature, arterial hypertension is a multifactorial disease with a significant genetic component; therefore, the study of pathogenic mechanisms of this disease depending on gene polymorphism of the angiotensin system, in particular AGTR1, is of special interest. This study involved 30 patients, in 15 of whom arterial hypertension was diagnosed as a component of metabolic syndrome while the remaining 15 ones had isolated arterial hypertension. The genotyping of patients was performed using the polymerase chain reaction and detection of amplification products by horizontal agarose gel electrophoresis. DNA was extracted from blood samples. All patients underwent 24-hour BP monitoring (ABPM). Two groups were formed according to the results of PCR: one consisted of patients with the AGTR1А1166А genotype (n=16) and the other included patients with the AGTR1А1166СorAGTR1С1166С genotypes (n=14). The groups were matched for age, sex, body-weight index (BWI), and waist circumference (WC). We studied the features of arterial hypertension, the clinical course including duration of AH, maximum and minimum values of systolic and diastolic blood pressure during 24 hours, the degree of night-time drop in BP, and effects of antihypertensive therapy. It was shown that systolic blood pressure, duration of the disease, comorbidity, effects of the treatment, the frequency of metabolic syndrome were significantly different in the two groups. It was concluded that hypertension and metabolic syndrome may develop synchronously in patients with the C allele of the AGTR1 gene.

scholarly journals Irbesartan prevents sodium channel remodeling in a canine model of atrial fibrillation

2018 ◽  
Vol 19 (1) ◽  
pp. 147032031875526 ◽  
Author(s):  
Xuewen Wang ◽  
Guangping Li
Keyword(s):  
Atrial Fibrillation ◽  
Sodium Current ◽  
Renin Angiotensin System ◽  
Canine Model ◽  
Atrial Pacing ◽  
Chain Reaction ◽  
Patch Clamp Technique ◽  
Angiotensin System ◽  
Whole Cell Patch Clamp ◽  
Polymerase Chain

Introduction: Activation of the renin-angiotensin system (RAS) plays an important role in atrial electrical remodeling (AER). The purpose of the present study was to evaluate the effects of irbesartan on cardiac sodium current (INa) in a canine model of atrial fibrillation. Materials and methods: Eighteen dogs were randomized into sham, pacing or pacing+irbesartan groups ( n = 6 in each group). The dogs in the pacing and irbesartan group were paced at 500 bpm for two weeks. Irbesartan (60 mg·kg−1·d−1) was administered orally in the pacing+irbesartan groups. INa was recorded using the whole-cell patch clamp technique from canine atrial myocytes. The expressions of cardiac Na+ channels (Nav1.5) mRNA were semi-quantified by reverse transcription-polymerase chain reaction. Results: Our results showed that INa density and Nav1.5 mRNA expression in the pacing group decreased significantly ( p < 0.05 vs. sham). However, rapid atrial pacing had no effects on the half-activation voltage (V1/2act) and half-inactivation voltage (V1/2inact) of INa ( p > 0.05 vs. sham). Irbesartan significantly increased INa densities and gene expression and hyperpolarized V1/2act without concomitant changes in V1/2inact. Conclusions: Irbesartan significantly increased INa densities, which contributed to improving intra-atrial conduction and prevented the induction and promotion of AF in atrial pacing dogs.

scholarly journals Association of ACE DD Genotype with Hypertension among the Tribal Populations of South India

2016 ◽  
Vol 52 ◽  
pp. 1-8 ◽  
Author(s):  
Raghu Paramasivam ◽  
Nandhakumar Rengasamy ◽  
Deva Arumugam ◽  
Prabhakaran Krishnan
Keyword(s):  
South India ◽  
Renin Angiotensin System ◽  
Chain Reaction ◽  
Specific Primers ◽  
Angiotensin System ◽  
Allele Specific ◽  
Tribal Populations ◽  
Important Regulator ◽  
Polymerase Chain ◽  
Vasoactive Peptide

The Renin-Angiotensin System (RAS) is an important regulator of the blood pressure (BP). The level of the vasoactive peptide Angiotensin-II, is mainly determined by the RAS enzyme, angiotensin converting enzyme-1 (ACE-1). Polymorphisms in ACE gene is reported to be associated with hypertension in various populations worldwide. We investigated the association of ACE I/D polymorphisms with hypertension among the tribal populations of South India. Samples were collected from hypertensive patients (n = 33) and healthy controls (n = 37). Genotyping was performed using Polymerase chain reaction (PCR) with allele specific primers. The DD genotype is significantly observed among the cases (OR = 1.0). Specifically, the DD genotype is more evident among the females (OR = 0 .705) than males (OR = 1.22) and is analysed to be associated with hypertension among the tribal populations of South India.

Features of virus infection COVID-19 during the pregnancy

2020 ◽  
Vol 18 (6) ◽  
pp. 20-23
Author(s):  
D. I. SHEVELEVA ◽  
◽  
A. V. ROMANOVSKAYA ◽  
N. F. KHVOROSTUKHINA ◽  
◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Lower Respiratory Tract ◽  
Clinical Course ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Gestational Stage ◽  
Coronavirus Infection ◽  
Polymerase Chain

The purpose of the research was to study the features of the clinical course of COVID-19 viral infection in pregnant women, depending on the gestational stage. Material and methods. Included the study of 67 pregnant women with COVID-19 at different periods of gestation. The age of women ranged from 16 to 41 years. All pregnant women were hospitalized to the infectious diseases departments of Saratov hospitals. During the period of hospitalization, the examination of women was carried out according to the methodological recommendations of the Ministry of Health of Russia for coronavirus infection in pregnant women. During virological examination of mucus from the pharynx and nose, using the reverse transcription polymerase chain reaction method, the RNA of the COVID-19 virus was found in all pregnant women. Results. COVID-19 most frequently infects primipara in the I (61,6%) and III (70,6%) trimester of gestation and multipara in the II (62,5%) trimester of gestation with burdened obstetrics-gynecological anamnesis (58,3%) and accompanying pathologies (41,5%). In pregnant women with COVID-19, mild and intermediate forms of the disease prevailed (31,3% and 47,8% respectiely). The frequency of coronavirus infection in the I (38,8%) and II (35,8%) trimesters of gestation increases the frequency of that in the III (25,3%) trimester. Among women with COVID-19, the following symptoms were observed: decrease in smell and taste in 66 (98,5%) cases, sore throat in 59 (85%) cases, increased body temperature in 61 (90,9%) cases. Lesions of lower respiratory tract was observed in all stages of gestation, pneumonias were less frequent in the I trimester. In all cases the COVID-19 course had a favorable outcome, with no lethality.

scholarly journals Is perinatal CoVID-19 possible: first results

2020 ◽  
Vol 12 (3) ◽  
pp. 51-55
Author(s):  
M. A. Vashukova ◽  
V. A. Zinserling ◽  
N. Yu. Semenova ◽  
N. A. Lugovskaya ◽  
T. A. Narkevich ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Structural Changes ◽  
Clinical Course ◽  
Spike Protein ◽  
Chain Reaction ◽  
Single Observation ◽  
First Results ◽  
Polymerase Chain ◽  
Transplacental Infection ◽  
Comprehensive Study

Among the problems associated with a new coronavirus infection, the possibility of its occurrence in pregnant women plays an important role. Until now, there is very little data on perinatal COVID-19, and there are no descriptions of structural changes in the afterbirth at all. Material and methods. A clinical and morphological analysis of 6 cases in which women with verified COVID-19 gave birth in an infectious hospital was performed. In all cases, the placenta was examined in detail morphologically using antibodies to the nucleoprotein and spike (S1 subunit spike protein) SARS-CoV-2. COVID-19 Results. It is shown that the clinical course of COVID-19 in pregnant women may be different, three were in the intensive care unit, including one was shown to be on a ventilator. 4 children were born at term, 2 children prematurely (at 31- 33 weeks of pregnancy). The condition of children in 5 nab. was assessed as satisfactory, only one child in serious condition was transferred to a children’s hospital. Intrauterine infection with the polymerase chain reaction documented in a single observation. When histological examination of the afterbirth in all observations, both in the maternal and fetal parts, changes characteristic of RNA viral infection was detected. Both of the studied antigens were found in moderate amounts in IHC. Conclusions. Intrauterine transplacental infection with a new coronavirus is certainly possible. Its frequency and clinical significance require further comprehensive study.

Stretch-mediated activation of cardiac renin gene

1994 ◽  
Vol 267 (4) ◽  
pp. H1630-H1636 ◽  
Author(s):  
P. H. Boer ◽  
M. Ruzicka ◽  
W. Lear ◽  
E. Harmsen ◽  
J. Rosenthal ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Renin Angiotensin System ◽  
Volume Overload ◽  
Mrna Levels ◽  
Shunt Surgery ◽  
Chain Reaction ◽  
Angiotensin System ◽  
Renin Gene ◽  
Renin Mrna ◽  
Polymerase Chain

This study was designed to quantitate cardiac mRNA levels encoding components of the local renin-angiotensin system during the development of volume overload-induced cardiac hypertrophy. Changes in cardiac renin mRNA levels were measured in relation to renin activity in the left ventricle (LV) and in plasma after acute passive stretch of the heart caused by an aortovenocaval shunt in the rat. A quantitative reverse-transcriptase polymerase chain reaction method with competitive internal standards was used to measure mRNA levels in total RNA derived from cardiac tissues after shunt. Seven days after shunt surgery, LV weight was increased by 23%. Renin activities were elevated four- and twofold in plasma and LV, respectively. LV angiotensinogen mRNAs were not significantly increased by shunt surgery; they were twofold higher than phosphoglycerate kinase mRNA from the housekeeping gene PGK-1. By day 7, LV levels for renin mRNA were significantly increased from well below 0.25% to approximately 1% of PGK-1 mRNA. Identity between renin polymerase chain reaction products from kidney and heart cDNAs and absence of "reninlike" amplification products were supported by Southern blotting. Volume overload caused increased expression of the renin gene in the stretched myocardium. This finding is consistent with the concept of a myocardial renin-angiotensin system that can be activated by locally produced renin and contributes to the hypertrophy of cardiac muscle.

scholarly journals Intermittent Hypoxia Upregulates the Renin and Cd38 mRNAs in Renin-Producing Cells via the Downregulation of miR-203

2021 ◽  
Vol 22 (18) ◽  
pp. 10127
Author(s):  
Yoshinori Takeda ◽  
Asako Itaya-Hironaka ◽  
Akiyo Yamauchi ◽  
Mai Makino ◽  
Sumiyo Sakuramoto-Tsuchida ◽  
...  
Keyword(s):  
Intermittent Hypoxia ◽  
Sleep Apnea Syndrome ◽  
Renin Angiotensin System ◽  
Negative Control ◽  
Target Sequence ◽  
Mrna Levels ◽  
Chain Reaction ◽  
Angiotensin System ◽  
Cyclic Adp Ribose ◽  
Polymerase Chain

Sleep apnea syndrome is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), and it is a known risk factor for hypertension. The upregulation of the renin-angiotensin system has been reported in IH, and the correlation between renin and CD38 has been noted. We exposed human HEK293 and mouse As4.1 renal cells to experimental IH or normoxia for 24 h and then measured the mRNA levels using a real-time reverse transcription polymerase chain reaction. The mRNA levels of Renin (Ren) and Cd38 were significantly increased by IH, indicating that they could be involved in the CD38-cyclic ADP-ribose signaling pathway. We next investigated the promotor activities of both genes, which were not increased by IH. Yet, a target mRNA search of the microRNA (miRNA) revealed both mRNAs to have a potential target sequence for miR-203. The miR-203 level of the IH-treated cells was significantly decreased when compared with the normoxia-treated cells. The IH-induced upregulation of the genes was abolished by the introduction of the miR-203 mimic, but not the miR-203 mimic NC negative control. These results indicate that IH stress downregulates the miR-203 in renin-producing cells, thereby resulting in increased mRNA levels of Ren and Cd38, which leads to hypertension.

scholarly journals Analysis of the input of polymorphism of several candidate genes in the development of hypertension and myocardial remodeling: in the sample of sibling pairs

2011 ◽  
Vol 17 (5) ◽  
pp. 488-492
Author(s):  
N. R. Khasanov ◽  
V. N. Oslopov ◽  
P. A. Slominskiy
Keyword(s):  
Renin Angiotensin System ◽  
Myocardial Remodeling ◽  
Myocardial Mass ◽  
Chain Reaction ◽  
Angiotensin System ◽  
Hypertensive Patients ◽  
Sibling Pairs ◽  
Polymerase Chain ◽  
Design And Methods ◽  
Agt Gene

Objective. To assess the contribution of the renin-angiotensin system, lipid metabolism and gene regulators of apoptosis in the development of hypertension and myocardial remodeling using sibling-pair and associative methods. Design and methods. Myocardial mass and myocardial mass index were calculated by echocardiography method, gene polymorphism was identified by polymerase chain reaction. Results. We found the association of genotype M235M AGT gene with hypertension in representatives of the Tatar ethnic group. Genotype A603A gene EAAT2 is associated with higher systolic blood pressure, and genotype CC rs189994 AIF gene is related to the greater myocardial mass in hypertensive patients.

Sign in / Sign up

Export Citation Format

Share Document