Palynological Study on Some Grape (Vitis vinifera L.) Cultivars Using Scanning Electron Microscopy

A family-wide palynological study of Myrtaceae was conducted using scanning electron microscopy (SEM) and light microscopy (LM). In this part of the study, the pollen morphology of 18 genera and 150 species from the Myrtaceae tribes of subfamily Myrtoideae, Eucalypteae, Lophostemoneae, Syncarpieae, Xanthostemoneae and subfamily Psiloxyloideae are presented. It was found that the most commonly observed pollen in these groups was parasyncolpate with a rugulate exine, whereas some species possessed an apocolpial island. The large, and sometimes syndemicolpate, pollen of Eucalypteae genera Angophora and Corymbia differed from all other genera. Most Eucalyptus pollen had endopores with a thickened exine.

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Pollen morphology of Prototulbaghia Vosa: A comparative palynological study of the Southern African Alliaceae.

Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie ◽

10.4102/satnt.v32i1.389 ◽

2013 ◽

Vol 32 (1) ◽

Cited By ~ 1

Author(s):

Melissa Andriessen ◽

Madeleen Struwig ◽

Stefan J. Siebert

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pollen Morphology ◽

Pollen Grain ◽

Evolutionary Significance ◽

Monotypic Genus ◽

Taxonomic Characteristic ◽

Unknown Event ◽

Palynological Study ◽

Scanning Electron

The Southern African Alliaceae Borkh. is represented by four genera (Allium L., Nothoscordum Kunth, Tulbaghia L. and Prototulbaghia Vosa) and 28 species. The pollen morphology of the endangered monotypic genus Prototulbaghia has not been described before. A comparative study of the pollen morphology of Prototulbaghia siebertii Vosa, Nothoscordum borbonicum Kunth, Tulbaghia simmleri P.Beauv. and T. violaceae Harv. is presented in this article. Scanning electron microscopy, as well as light microscopy, were used to examine the pollen. The pollen morphology of the species can be described as perprolate and monosulcate, and the surface sculpture as reticulate and heterobrochate. However, the pollen of Prototulbaghia siebertii displays a unique characteristic as the grains are folded in their breadth with the tips touching, hence causing the grain to display a triangular and disulcate appearance. It might be possible to ascribe this fold to the process of harmomegathy or a still unknown event that occurs during the development of the pollen grain. This phenomenon should be further investigated to determine the cause of folding and whether it is a unique taxonomic characteristic of this genus, and if it could be of evolutionary significance for the Alliaceae.

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Comparative scanning electron microscopy investigations on some characteristics of the pollen aperture complex in seedless grape Vitis vinifera L. cultivars

OENO One ◽

10.20870/oeno-one.1996.30.1.1115 ◽

1996 ◽

Vol 30 (1) ◽

pp. 1

Author(s):

Slavcho Pandeliev ◽

Venelin Roytchev

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Statistical Treatment ◽

Polar Axis ◽

Mathematical Treatment ◽

Pollen Surface ◽

Seedless Grape ◽

Palynological Study ◽

Grape Cultivars ◽

Scanning Electron

In the 1989-1994 period, a palynological study of 66 seedless grape cultivars, varieties and hybrid vine forms was made. On the basis of scanning electron microscopy observations on the pollen aperture complex elements and on the statistical treatment of the results obtained, the following conclusions were drawn :1 - In seedless grape cultivars, characterized by functionally male type of flowers, there are no significant differences in the shape of colps and the surface structures on the pollen aperture complex.2 - The classification of seedless cultivars according to pollen surface is arbitrary and the differences in the parameters are not confirmed by the mathematical treatment of aperture elements.3 - The mean dimensions of the most important pollen aperture complex elements in seedless grape cultivars are the following: polar axis: 23,48 μm ; equatorial axis: 15,37 μm ; mesocolpium : 11,61 μm ; apocolpium : 4,85 μm, colplength : 20,93 μm ; colp width : 0,92 μm, colpdepth : 0,60 μm.

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Caracterización del agente causante de la deformación de los frutos de la uva (Vitis vinifera L.) var. Red Globe en La Unión, Valle del Cauca, Colombia

Revista de la Academia Colombiana de Ciencias Exactas Físicas y Naturales ◽

10.18257/raccefyn.844 ◽

2019 ◽

Vol 43 (167) ◽

pp. 241

Author(s):

Silvia Patricia López-Zapata ◽

Jairo Castaño-Zapata ◽

Rafael Arango-Isaza ◽

Dayana Andrea Vásquez-Barajas

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Internal Transcribed Spacer ◽

Colletotrichum Gloeosporioides ◽

Environmental Scanning Electron Microscopy ◽

Environmental Scanning ◽

Vitis Vinifera L ◽

Para Determinar ◽

Valle Del Cauca ◽

Scanning Electron

En años recientes una enfermedad caracterizada por la necrosis y el hundimiento de la epidermis de las bayas de la vid (Vitis viinifera L.) se ha venido presentando en la variedad Red Globe, cultivada en predios vitícolas del municipio de La Unión, Valle del Cauca, lo que ha resultado en pérdidas de rendimiento y calidad. Para determinar la etiología de la enfermedad, se recolectaron y procesaron frutos que mostraban los signos típicos de decoloración y posterior necrosis. Con mayor prevalencia se encontró un hongo, que fue sometido a pruebas de patogenicidad y caracterización morfológica mediante microscopía de luz y electrónica (Environmental scanning electron microscopy, ESEM), complementadas con pruebas moleculares. Los postulados de Koch se cumplieron mediante la inoculación de una suspensión conidial de 1x106 conidios por mL-1 de agua en bayas sanas de la misma variedad. Al cabo una semana se empezaron a observar signos similares a los observados en campo. Las búsquedas de similitud con la herramienta BLAST mostraron una identidad del 100 % entre las secuencias del espaciador transcribible interno (internal transcribed spacer, ITS) y Colletotrichum aenigma y C. siamense, pertenecientes al complejo de especies Colletotrichum gloeosporioides, lo que proporciona información útil para entender la enfermedad de las bayas de la vid y poder diseñar estrategias de manejo. © 2019. Acad. Colomb. Cienc. Ex. Fis. Nat.

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Pathogenesis of Human Hair Defects

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005007x ◽

1974 ◽

Vol 32 ◽

pp. 12-13

Author(s):

P.S. Porter ◽

T. Aoyagi ◽

R. Matta

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Human Hair ◽

Standard Techniques ◽

Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

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Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080614 ◽

1977 ◽

Vol 35 ◽

pp. 638-639

Author(s):

P.J. Dailey

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Salivary Glands ◽

Secretory Vesicles ◽

The Past ◽

Transmission Electron ◽

Means Of Transmission ◽

Gromphadorhina Portentosa ◽

Scanning Electron ◽

Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081279 ◽

1978 ◽

Vol 36 (2) ◽

pp. 82-83 ◽

Cited By ~ 1

Author(s):

Nakazo Watari ◽

Yasuaki Hotta ◽

Yoshio Mabuchi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Transmission Electron Microscopy ◽

Fine Structure ◽

Light Microscopy ◽

Thin Sections ◽

Transmission Electron ◽

Identical Cell ◽

Electron Microscopes ◽

Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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Spontaneous Cataract in Nude Athymic Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079991 ◽

1977 ◽

Vol 35 ◽

pp. 512-513

Author(s):

Ronald H. Bradley ◽

R. S. Berk ◽

L. D. Hazlett

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Nude Mouse ◽

Cellular Immunity ◽

Phosphate Buffer ◽

Osmium Tetroxide ◽

Athymic Mice ◽

Transmission Microscopy ◽

Mouse Lens ◽

Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066097 ◽

1971 ◽

Vol 29 ◽

pp. 410-411 ◽

Cited By ~ 1

Author(s):

Jane A. Westfall ◽

S. Yamataka ◽

Paul D. Enos

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Osmium Tetroxide ◽

External Surface ◽

Three Dimensional ◽

Surface Structures ◽

Transmission Microscopy ◽

Transmission Electron ◽

Freeze Dried ◽

Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

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