A new in vitro model to study interaction between whole blood and biomaterials. Studies of platelet and coagulation activation and the effect of aspirin

Biomaterials ◽  
1999 ◽  
Vol 20 (7) ◽  
pp. 603-611 ◽  
Author(s):  
Jaan Hong ◽  
Kristina Nilsson Ekdahl ◽  
Helena Reynolds ◽  
Rolf Larsson ◽  
Bo Nilsson
Keyword(s):  
Whole Blood ◽  
In Vitro Model ◽  
Coagulation Activation ◽  
Vitro Model

THE EFFECT OF COD LIVER OIL INTAKE ON THE STIMULATION OF BLOOD CELLS

1987 ◽  
Author(s):  
J B Hansen ◽  
J O Olsen ◽  
L Wilagård ◽  
B Østerud
Keyword(s):  
Platelet Aggregation ◽  
Young Women ◽  
Whole Blood ◽  
Blood Cells ◽  
In Vitro Model ◽  
Cod Liver Oil ◽  
Blood Samples ◽  
Vitro Model ◽  
Stimulation Of

In an in vitro model, stimulation of blood cells with a low concentration of lipopolysaccharides (LPS) revealed differences between women and men that possibly could be an explanation to why young women have less coronary heart disease than men (see abstract Hansen et al. “A model to--”).This model was also used to study the effect of intake of cod liver oil (CLO). 40 students (20 men and 20 women) were tested followed by an intake of 25 ml CLO daily for 2 months by 20 of the students.Heparinized blood samples were incubated with 2 ng LPS/ ml for 2 hours followed by isolation of plasma for thromboxane B2 and 6-keto-PG 1α quantitation.After the first 2 months period of CLO drinking we have the following results:The two months of CLO intake had no significant effect pn the thromboplastin induced synthesis in monocytes. In addition platelet aggregation was tested in a whole blood aggregometer using ADP addition to heparinized blood or collagen induced platelet aggregation in citrated whole blood. ADP aggregation was reduced from 75.9 ± 16.8% to 55.4 ± 19% in the CLO group of women, whereas the reduction in the CLO group of men was 70.1 ± 17.1% to 60.9±18.6%. Similar result were found with collagen aggregation (57% to 33% for women and 48% to 30% for men).It is concluded that CLO intake reduces TxA2 production and plateletaggregation without having reduced effect on PGI2 production in whole blood.

Endothelial cell protection and complement inhibition in xenotransplantation: a novel in vitro model using whole blood

2005 ◽  
Vol 12 (6) ◽  
pp. 434-443 ◽  
Author(s):  
Yara Banz ◽  
Trinh Cung ◽  
Elena Y. Korchagina ◽  
Nicolai V. Bovin ◽  
André Haeberli ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Whole Blood ◽  
In Vitro Model ◽  
Cell Protection ◽  
Complement Inhibition ◽  
Vitro Model

scholarly journals Evaluation the Effects of some Cobalt Amino Acids Complexes Using Leukocytes as in vitro Model for Cytotoxicity

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Andreea STĂNILĂ ◽  
Cornelia BRAICU
Keyword(s):  
Amino Acids ◽  
Lipid Peroxidation ◽  
Amino Acid ◽  
Whole Blood ◽  
In Vitro Model ◽  
Cobalt Complexes ◽  
Prooxidant Effect ◽  
Amino Acid Complexes ◽  
Vitro Model

It was evaluate the effect of amino acids complex we had used viable leukocytes readily obtained from sterile whole blood as an in vitro model for cytotoxicity. The end point for cytotoxicity evaluation was lactate dehidrogenase activity (LDH) and lipid peroxidation (MDA-TBA test). We tested 5 amino acid complexes: Co-leucine, Co-methionine, Co-valine, Co-hystidine, Co-phenylalanine at different concentrations. Some of the amino acids complexes determined the decreasing of LDH level after 8h, 24h and 48h which mean that these compounds have no cytotoxicity. Concerning the lipid peroxidation the lowest level were obtained for cobalt complexes with metionine, valine, leucine and hystidine at the concentrations of 2-0,2µM and for cobalt phenylalanine complexes for all concentrations especially after 24h and 48h. The higher levels of of lipid peroxidation were in the case of Copper-valine at 2µM and 20µM after 24h, Copper-hystidine at 20µM after 8h, 24h, 48h, and Co-leucine at 20µM after 48h, having a prooxidant effect.

scholarly journals Microparticle formation after co-culture of human whole blood and umbilical artery in a novel in vitro model of flow

2011 ◽  
Vol 81A (5) ◽  
pp. 390-399 ◽  
Author(s):  
Emma Holtom ◽  
James R. Usherwood ◽  
Marion G. Macey ◽  
Charlotte Lawson
Keyword(s):  
Whole Blood ◽  
In Vitro Model ◽  
Umbilical Artery ◽  
Human Whole Blood ◽  
Vitro Model ◽  
Microparticle Formation

An In Vitro Method for Screening Compounds for the Effect on the Rate of Sickling of Erythrocytes

1976 ◽  
Vol 4 (6) ◽  
pp. 375-381 ◽  
Author(s):  
R D Mackenzie ◽  
E M Gleason ◽  
G L Schatzman ◽  
M J Cawein
Keyword(s):  
Whole Blood ◽  
Calcium Concentration ◽  
In Vitro Model ◽  
Inhibitory Effect ◽  
Calcium Flux ◽  
In Vitro System ◽  
Oxygen Dissociation ◽  
Vitro Model

Since no practical animal model is available for the evaluation of compounds in vivo, we have developed an in vitro model for determining the effect of compounds on the rate of sickling of erythrocytes in whole blood taken from patients with sickle cell anaemia. RMI 6792 (a phenethanol-diamine derivative), procaine, and L-glutamine were tested in this in vitro system. RMI 6792 was tested at various concentrations in whole blood. The data indicate that RMI 6792 decreased the rate of sickling at and above 60 μg/ml. Procaine slightly decreased sickling rate at 100 μg/ml. L-glutamine at 555 μg/ml had no inhibitory effect. RMI 6792 and procaine had no effect on the oxygen dissociation curve. RMI 6792 affected the calcium flux of the erythrocytes and the calcium concentration in the erythrocytes.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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