scholarly journals Cryopreservation of Vasconcellea quercifolia St.-Hil (Caricaceae) Zygotic Embryos

2018 ◽  
Vol 47 (1) ◽  
pp. 63-69
Author(s):  
Cibele M. GALLO ◽  
Renato PAIVA ◽  
Rodrigo T. FREITAS ◽  
Michele V. REIS ◽  
Diogo P.C. SILVA ◽  
...  
Keyword(s):  
Silica Gel ◽  
Growth Analysis ◽  
Seedling Development ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Papaya Ringspot Virus ◽  
Breeding Programs ◽  
Tropical Fruit ◽  
Fruit Species ◽  
Growth Room

Vasconcellea quercifolia A. St.-Hil. (Caricaceae) is a tropical fruit species native to Brazil, with a great importance in plant breeding programs. The V. quercifolia has a resistance to the main diseases of Caricaceae, Papaya Ringspot Virus (PRSV). Considering its potential, cryopreservation becomes a tool for the conservation of this species. The objective of this paper was to study the cryopreservation of V. quercifolia zygotic embryos through dehydration in silica gel. Excised zygotic embryos were dehydrated in silica at 0, 20, 40, 80 and 100 minutes and then inoculated in MS medium. The percentage of germinated and recovered embryos, and growth analysis were evaluated, besides water content. Subsequently, they were acclimatized in a growth room with temperature controlled. For cryopreservation, the embryos were excised and dehydrated in silica for 0, 20, 40 and 60 minutes, immersed in Liquid Nitrogen (LN) for 1 hour, thawed in Recovery Solution (RS) and inoculated in MS medium. After 30 days, the percentage of germinated and recovered embryos was evaluated. The silica gel promotes a fast dehydrate of embryos. The results showed that embryo dehydration affected seedling development, and dehydration for over 20 minutes showed a reduction in all evaluated parameters. The plantlets regenerated from embryos dehydrate survive the acclimatization. It was possible to cryopreserve the V. quercifolia zygotic embryos when the dehydration time of 20 minutes by silica gel was used.

scholarly journals In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Hoehnea ◽  
2018 ◽  
Vol 45 (4) ◽  
pp. 663-668
Author(s):  
Daniel da Silva ◽  
Angela Maria Imakawa ◽  
Suely de Souza Costa ◽  
Paulo de Tarso Barbosa Sampaio
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Germination Rate ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Growth Room ◽  
Morphogenetic Responses

ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

scholarly journals Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

2016 ◽  
Vol 22 (3) ◽  
pp. 343
Author(s):  
Antonio Anderson De Jesus Rodrigues ◽  
Eder De Oliveira Santos ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Previous Experiment ◽  
Economic Importance ◽  
Zygotic Embryos ◽  
In Vitro Cultivation ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Induction ◽  
Growth Room ◽  
Tropical Flowers

Considering the growing economic importance of tropical flowers and the advantages of techniques applied to the in vitro cultivation of these plants, it is necessary to carry out studies to evaluate growth in species such as Heliconia latispatha. The aim of this study therefore, was to evaluate in vitro shoot induction for different concentrations of BAP and NAA and as a function of the photoperiod. Explants from zygotic embryos were inoculated in MS medium containing different concentrations of BAP (0.0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L-1), with the cultures kept in a growth room at a temperature of 24.0 ± 2.0° C, under a photoperiod of 12 and 16 hours of light and a light intensity of 30 μmol m-2 s-1. At 21, 28, 35, 42 and 49 days after inoculation, the number of shoots per explant was evaluated. The treatment at the BAP concentration that gave the best multiplication rate (2.5 mg L-1) was set, and was tested in a further trial with different concentrations of NAA (0.0, 0.2, 0.4, 0 6, 0.8 or 1.0 mg L-1) under the same conditions as the previous experiment. The experimental designs were completely randomised, with five replications, and analysed in a 6 x 2 factorial. The data were submitted to analysis of variance and regression. No significant differences were seen in relation to the photoperiod or its interaction with the cytokinin and auxin under test. Multiplication was greater in the presence of 2.5 mg L-1 BAP, which gave a rate of 1.25 shoots/explant at 49 days of in vitro culture. The association of this BAP dosage with 1.0 mg L-1 NAA was even more efficient, producing 1.83 shoots per explant at 30 days of growth. The use of BAP together with NAA is beneficial to the induction of shoots in H. latispatha.

scholarly journals In vitro Embryo Morphogenesis and Micropropagation of Dendrobium aggregatum Roxb.

2014 ◽  
Vol 23 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Mohammad Musharof Hossain
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Developmental Stages ◽  
Cell Mass ◽  
Multiple Shoot ◽  
Seedling Development ◽  
Ms Medium ◽  
Multiple Shoot Buds ◽  
Half Strength

In vitro embryo morphogenesis and micropropagation of Dendrobium aggregatum Roxb. were described. The gradual developmental stages of embryos to seedlings were traced out. Within two weeks of culture the cells of undifferented embryos underwent repeated aniclinal and periclinal division producing a compact, green parenchymatous cell mass called spherule that emerged out by rupturing the testa. The spherules subsequently differentiated into greenish protocorms were considered as typical seed germination. Germination occurred on both (MS and Phytamax (PM) medium but MS medium proved to be more efficient. The primary protocorms underwent profuse proliferation through production of secondary (2º) protocorms when transferred to different plant growth regulators (PGRs) supplemented MS; the medium fortified with 2.0 mg/l BAP and 1.0 mg/l NAA proved to be most effective for induction of 2º protocorms and seedling development. Multiple shoot buds (MSBs) were induced in pseudobulb segments of the in vitro grown seedlings when cultured on different PGRs supplemented media; and the maximum number of MSBs were obtained MS + 2.0 mg/l BAP + 0.5 mg/l picloram. The MSBs underwent elongation and then they rooted when they were transferred to half strength of MS + 0.5 mg/l IAA. The well rooted plantlets were finally transferred to outside natural environment with 80% survival. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17525 Plant Tissue Cult. & Biotech. 23(2): 241-249, 2013  (December)

scholarly journals Resource use and GHG emissions of eight tropical fruit species cultivated in Colombia

Fruits ◽  
2013 ◽  
Vol 68 (4) ◽  
pp. 303-314 ◽  
Author(s):  
Sophie Graefe ◽  
Jeimar Tapasco ◽  
Alonso Gonzalez
Keyword(s):  
Resource Use ◽  
Ghg Emissions ◽  
Tropical Fruit ◽  
Fruit Species

scholarly journals Optimization of a protocol for the micropropagation of pineapple

2002 ◽  
Vol 24 (2) ◽  
pp. 296-300 ◽  
Author(s):  
WELITON ANTONIO BASTOS DE ALMEIDA ◽  
GESSIONEI SILVA SANTANA ◽  
ADRIANA PINHEIRO MARTINELI RODRIGUEZ ◽  
MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Sterile Water ◽  
Calcium Hypochlorite ◽  
Shoot Bud ◽  
Explant Source ◽  
Growth Room

The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1), 27 ± 2ºC. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L-1), and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L-1, associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months.

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

RESPONSE OF ONION (Allium cepa) TO OXYFLUORFEN DURING EARLY SEEDLING DEVELOPMENT

1985 ◽  
Vol 65 (2) ◽  
pp. 357-362 ◽  
Author(s):  
W. C. AKEY ◽  
V. SOUZA MACHADO
Keyword(s):  
Allium Cepa ◽  
Dry Weight ◽  
Epicuticular Wax ◽  
Seedling Development ◽  
Allium Cepa L ◽  
Growth Room ◽  
Early Seedling ◽  
Growing Conditions ◽  
Relationship Of ◽  
Early Seedling Development

The response of onion (Allium cepa L.) to postemergence applications of oxyfluorfen during early seedling development was measured in growth room experiments. Tolerance to oxyfluorfen increased more than 70-fold from the late loop to the two fully developed leaf stage. Field and growth room experiments were also conducted to determine the relationship of spray retention and epicuticular wax on the tolerance of onion seedlings to the herbicide. In general, there was a progressive decrease in spray retention and a corresponding increase in epicuticular wax per unit dry weight as plant age increased. Spray retention in the field was approximately twice as great as in the growth room. The amount of epicuticular wax on the leaf surfaces of the onions was about equivalent under the two growing conditions. A significant decrease in epicuticular wax per unit dry weight between the late one-leaf and late two-leaf stages occurred under both field and growth room conditions, while tolerance to the herbicide continued to increase during the same period.Key words: Onions, oxyfluorfen, ED50, spray retention, epicuticular wax

Sign in / Sign up

Export Citation Format

Share Document