scholarly journals Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

2016 ◽  
Vol 22 (3) ◽  
pp. 343
Author(s):  
Antonio Anderson De Jesus Rodrigues ◽  
Eder De Oliveira Santos ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Previous Experiment ◽  
Economic Importance ◽  
Zygotic Embryos ◽  
In Vitro Cultivation ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Induction ◽  
Growth Room ◽  
Tropical Flowers

Considering the growing economic importance of tropical flowers and the advantages of techniques applied to the in vitro cultivation of these plants, it is necessary to carry out studies to evaluate growth in species such as Heliconia latispatha. The aim of this study therefore, was to evaluate in vitro shoot induction for different concentrations of BAP and NAA and as a function of the photoperiod. Explants from zygotic embryos were inoculated in MS medium containing different concentrations of BAP (0.0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L-1), with the cultures kept in a growth room at a temperature of 24.0 ± 2.0° C, under a photoperiod of 12 and 16 hours of light and a light intensity of 30 μmol m-2 s-1. At 21, 28, 35, 42 and 49 days after inoculation, the number of shoots per explant was evaluated. The treatment at the BAP concentration that gave the best multiplication rate (2.5 mg L-1) was set, and was tested in a further trial with different concentrations of NAA (0.0, 0.2, 0.4, 0 6, 0.8 or 1.0 mg L-1) under the same conditions as the previous experiment. The experimental designs were completely randomised, with five replications, and analysed in a 6 x 2 factorial. The data were submitted to analysis of variance and regression. No significant differences were seen in relation to the photoperiod or its interaction with the cytokinin and auxin under test. Multiplication was greater in the presence of 2.5 mg L-1 BAP, which gave a rate of 1.25 shoots/explant at 49 days of in vitro culture. The association of this BAP dosage with 1.0 mg L-1 NAA was even more efficient, producing 1.83 shoots per explant at 30 days of growth. The use of BAP together with NAA is beneficial to the induction of shoots in H. latispatha.

scholarly journals In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Hoehnea ◽  
2018 ◽  
Vol 45 (4) ◽  
pp. 663-668
Author(s):  
Daniel da Silva ◽  
Angela Maria Imakawa ◽  
Suely de Souza Costa ◽  
Paulo de Tarso Barbosa Sampaio
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Germination Rate ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Growth Room ◽  
Morphogenetic Responses

ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

scholarly journals Multiplicação de bastão-do-imperador em resposta a concentrações de BAP e número de subcultivos

2016 ◽  
Vol 22 (1) ◽  
pp. 88
Author(s):  
Eder De Oliveira Santos ◽  
Antonio Anderson De Jesus Rodrigues ◽  
Esdras Rocha da Silva ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Experimental Design ◽  
Light Intensity ◽  
Analysis Of Variance ◽  
Culture Medium ◽  
Multiplication Rate ◽  
Commercial Cultivation ◽  
Growth Room ◽  
Tropical Flowers ◽  
Etlingera Elatior

The large ornamental potential of tropical flowers has stimulated the commercial cultivation of various species. Micropropagation is a viable alternate method of propagation, since it enables obtaining a higher number of seedlings with uniformity and pathogens free. The objective was to evaluate the in vitro multiplication rate of Etlingera elatior cv. Porcelana, using explants obtained from in vitro established seedling shoots, obtained from the 2nd subcultive. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 6.66; 8.88; and 11,10 μM), and the cultures maintained in a growth room with temperature 25.0 ± 2.0 °C under a photoperiod of 12 hours of light and light intensity of 30 μmol.m-2 s-1. The multiplication rate was monthly, according to the four subcultives, totaling 120 days. The experimental design was completely randomized, with four replications, analyzed in a factorial 4 x 6. The data were submitted to analysis of variance and regression. There were significant differences in subcultives and made for BAP concentrations used. For the first subcultive, the concentration of 2.22 μM of BAP afforded a rate of 4.06 sprouts per explant, already in the second and fourth subcultives, with the addition of cytokinin concentration was increased amount of sprouts reaching at a rate of 4.05 and 4.96 shoots/explant in the highest concentration of BAP. The results of the treatments evaluated indicate that the presence of BAP favored sprout emission. The concentrations of 2.22, 8.88 and 11.10 μM this cytokinin promoted the highest multiplication rates in the first, second and fourth subcultives, respectively.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Rosiane Cipriano ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Antelmo Ralph Falqueto ◽  
Andreia Barcelos Passos Lima Gontijo
Keyword(s):  
Photosystem Ii ◽  
Saline Solution ◽  
Growth Traits ◽  
Photosynthetic Apparatus ◽  
Solution Culture ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Positive Effect

Abstract In vitro cultivation is a technique with wide application for micropropagation. However, each species has specific mineral needs for this type of cultivation. The objective was to assess the impacts of the saline solution culture medium on the performance of the photosynthetic apparatus and growth of Bromelia antiacantha during in vitro cultivation, and thus to elucidate the mitigation of the nutritional imbalance that can interfere in the electron transport in the plants. Plants were cultivated in a salt concentration gradient of MS medium (0%, 25%, 50%, 75% or 100%). The growth traits and fluorescence a chlorophyll were analyzed. Intermediate concentrations of MS medium resulted in plants with a larger number of leaves and longer root length. The OJIP curves and results of the JIP test showed that the plants grown without MS salts presented less efficient photosystem II (PSII), as indicated by the performance index [Pi(total)]. In contrast, the intermediate concentrations (MS 25% and 50%) had a positive effect on the performance of the photosynthetic apparatus. The MS 25% medium can be used for in vitro cultivation of B. antiacantha, enabling the development of plants with suitable physiological qualities for planting in the field.

scholarly journals Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

2019 ◽  
Vol 13 (2) ◽  
pp. 269-278
Author(s):  
Adriano Bortolotti Silva ◽  
Ligiane Aparecida Florentino ◽  
Dalvana De Sousa Pereira ◽  
Paulo Roberto Correa Landgraf ◽  
Ana Carolina Rodrigues Alves ◽  
...  
Keyword(s):  
Shoot Growth ◽  
Ex Vivo ◽  
Control Treatment ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Indole 3 Acetic Acid

Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

scholarly journals In vitro Regeneration and Rapid Multiplication of Dendrobium bensoniae, an Indigenous Ornamental Orchid

2017 ◽  
Vol 14 (2) ◽  
pp. 24-31 ◽  
Author(s):  
S S Riva ◽  
A Islam ◽  
M E Hoque
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Rapid Multiplication ◽  
Number Of Shoots

An experiment was conducted on in vitro regeneration and multiplication of Dendrobium bensoniae. Different concentrations of BA and IBA alone or combination of both hormones were used as treatment for regeneration.  It was revealed that shoot regeneration from node was the best at 2.0 mg/l BA supplemented to MS medium. It gave better responses than all other concentrations and combinations of BA and BA+IBA, used in the present study. The highest number of shoots and leaves were found when 1.0 mg/l BA with 1.5 mg/l IBA was supplemented into MS medium.  For rooting, 0.5 mg/l BA with 1.0 mg/l IBA was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field. From the present result, it may be recommended that MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction and regeneration of D. bensoniae.The Agriculturists 2016; 14(2) 24-31

scholarly journals Cryopreservation of Vasconcellea quercifolia St.-Hil (Caricaceae) Zygotic Embryos

2018 ◽  
Vol 47 (1) ◽  
pp. 63-69
Author(s):  
Cibele M. GALLO ◽  
Renato PAIVA ◽  
Rodrigo T. FREITAS ◽  
Michele V. REIS ◽  
Diogo P.C. SILVA ◽  
...  
Keyword(s):  
Silica Gel ◽  
Growth Analysis ◽  
Seedling Development ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Papaya Ringspot Virus ◽  
Breeding Programs ◽  
Tropical Fruit ◽  
Fruit Species ◽  
Growth Room

Vasconcellea quercifolia A. St.-Hil. (Caricaceae) is a tropical fruit species native to Brazil, with a great importance in plant breeding programs. The V. quercifolia has a resistance to the main diseases of Caricaceae, Papaya Ringspot Virus (PRSV). Considering its potential, cryopreservation becomes a tool for the conservation of this species. The objective of this paper was to study the cryopreservation of V. quercifolia zygotic embryos through dehydration in silica gel. Excised zygotic embryos were dehydrated in silica at 0, 20, 40, 80 and 100 minutes and then inoculated in MS medium. The percentage of germinated and recovered embryos, and growth analysis were evaluated, besides water content. Subsequently, they were acclimatized in a growth room with temperature controlled. For cryopreservation, the embryos were excised and dehydrated in silica for 0, 20, 40 and 60 minutes, immersed in Liquid Nitrogen (LN) for 1 hour, thawed in Recovery Solution (RS) and inoculated in MS medium. After 30 days, the percentage of germinated and recovered embryos was evaluated. The silica gel promotes a fast dehydrate of embryos. The results showed that embryo dehydration affected seedling development, and dehydration for over 20 minutes showed a reduction in all evaluated parameters. The plantlets regenerated from embryos dehydrate survive the acclimatization. It was possible to cryopreserve the V. quercifolia zygotic embryos when the dehydration time of 20 minutes by silica gel was used.

scholarly journals Micropropagation and in vitro conservation of Alcantarea nahoumii (Bromeliaceae), an endemic and endangered species of the Brazilian Atlantic Forest

2020 ◽  
Vol 42 ◽  
pp. e52940
Author(s):  
Simone Sacramento dos Santos Silva ◽  
Everton Hilo de Souza ◽  
Fernanda Vidigal Duarte Souza ◽  
Cristina Ferreira Nepomuceno ◽  
Maria Angélica Pereira de Carvalho Costa
Keyword(s):  
Atlantic Forest ◽  
Culture Media ◽  
In Vitro Conservation ◽  
Naphthaleneacetic Acid ◽  
High Survival ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Mature Seeds ◽  
Stem Segments

Alcantarea nahoumii (Leme) J. R. Grant is a species native to the Atlantic Forest that stands out for ornamental purposes. The objective of this work was to evaluate the in vitro germination of A. nahoumii seeds and establish a micropropagation protocol for production of seedlings so as to minimize the effects of predatory extractivism and develop an in vitro conservation system. Mature seeds were disinfested, established in three culture media (MS, MS½ and MS⅓) and incubated at four temperatures (20, 25, 30 and 35ºC) in a germination chamber. In the micropropagation experiment, stem segments were introduced in MS medium supplemented with 0.5 μM of 1-naphthaleneacetic acid (NAA) and 0.0, 2.2, 4.4 and 6.6 μM of 6-benzylaminopurine (BAP). For the in vitro conservation, plantlets were established in MS or MS½ medium supplemented with 15 g L-1 or 30 g L-1 of sucrose. The plants were acclimated with commercial substrate. The highest seed germination percentages were promoted by temperature conditions of 20 and 25ºC, with MS culture medium. The highest multiplication rate of shoots was obtained from the treatment without addition of the growth regulator or when combined with 2.2 μM of BAP + 0.5 μM of NAA. The acclimation of the plants occurred with high survival rate. The species can be conserved in vitro under slow growth condition for 24 months when incubated in MS medium supplemented with 30 g L-1 of sucrose.

scholarly journals Micropropagation of Cyclopia genistoides, an Endemic South African Plant of Economic Importance

2012 ◽  
Vol 67 (1-2) ◽  
pp. 65-76 ◽  
Author(s):  
Adam Kokotkiewicz ◽  
Maria Luczkiewicz ◽  
Anna Hering ◽  
Renata Ochocka ◽  
Krzysztof Gorynski ◽  
...  
Keyword(s):  
South African ◽  
Sucrose Concentration ◽  
Shoot Multiplication ◽  
Potassium Nitrate ◽  
Economic Importance ◽  
Medium Composition ◽  
Root Induction ◽  
Multiplication Rate ◽  
Shoot Cultures

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 μM 6-(γ,γ-dimethylallylamino)purine (2iP) and 1.0 μM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 ± 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 μM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 μM indole-3-acetic acid (IAA) and 260.25 μM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the fl avanone hesperidin, characteristic of wild-growing shrubs.

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

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