scholarly journals Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish

2017 ◽  
Vol 89 (1 suppl) ◽  
pp. 661-674 ◽  
Author(s):  
DA HYE KWON ◽  
JIN WOO JEONG ◽  
EUN OK CHOI ◽  
HYE WON LEE ◽  
KI WON LEE ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Inflammatory Mediators ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inhibitory Effects

Inhibitory effects of fluvastain and its metabolites on the formation of several reactive oxygen species

Life Sciences ◽  
2001 ◽  
Vol 69 (12) ◽  
pp. 1381-1389 ◽  
Author(s):  
Akinori Nakashima ◽  
Masakatsu Ohtawa ◽  
Kazuhide Iwasaki ◽  
Mitsuhiro Wada ◽  
Naotaka Kuroda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inhibitory Effects

scholarly journals Mitochondrial reactive oxygen species: which ROS signals cardioprotection?

2013 ◽  
Vol 305 (7) ◽  
pp. H960-H968 ◽  
Author(s):  
Anders O. Garlid ◽  
Martin Jaburek ◽  
Jeremy P. Jacobs ◽  
Keith D. Garlid
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species ◽  
Inhibitory Effects ◽  
Physiol Heart Circ ◽  
Bromoenol Lactone ◽  
Rat Heart Mitochondria ◽  
Pharmacological Preconditioning

Mitochondria are the major effectors of cardioprotection by procedures that open the mitochondrial ATP-sensitive potassium channel (mitoKATP), including ischemic and pharmacological preconditioning. MitoKATP opening leads to increased reactive oxygen species (ROS), which then activate a mitoKATP-associated PKCε, which phosphorylates mitoKATP and leaves it in a persistent open state (Costa AD, Garlid KD. Am J Physiol Heart Circ Physiol 295, H874–H882, 2008). The ROS responsible for this effect is not known. The present study focuses on superoxide (O2·−), hydrogen peroxide (H2O2), and hydroxyl radical (HO˙), each of which has been proposed as the signaling ROS. Feedback activation of mitoKATP provides an ideal setting for studying endogenous ROS signaling. Respiring rat heart mitochondria were preincubated with ATP and diazoxide, together with an agent being tested for interference with this process, either by scavenging ROS or by blocking ROS transformations. The mitochondria were then assayed to determine whether or not the persistent phosphorylated open state was achieved. Dimethylsulfoxide (DMSO), dimethylformamide (DMF), deferoxamine, Trolox, and bromoenol lactone each interfered with formation of the ROS-dependent open state. Catalase did not interfere with this step. We also found that DMF blocked cardioprotection by both ischemic preconditioning and diazoxide. The lack of a catalase effect and the inhibitory effects of agents acting downstream of HO˙ excludes H2O2 as the endogenous signaling ROS. Taken together, the results support the conclusion that the ROS message is carried by a downstream product of HO˙ and that it is probably a product of phospholipid oxidation.

scholarly journals Increase of reactive oxygen species contributes to growth inhibition by fluconazole in Cryptococcus neoformans

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Nadir Hani Dbouk ◽  
Madison Bailey Covington ◽  
Kenny Nguyen ◽  
Srikripa Chandrasekaran
Keyword(s):  
Reactive Oxygen Species ◽  
Growth Inhibition ◽  
Cryptococcus Neoformans ◽  
Reactive Oxygen ◽  
Ergosterol Biosynthesis ◽  
Complex Nature ◽  
Pyrrolidine Dithiocarbamate ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Inhibitory Effects

Abstract Background Cryptococcus neoformans, a basidiomycetous yeast, is a fungal pathogen that can colonize the lungs of humans causing pneumonia and fungal meningitis in severely immunocompromised individuals. Recent studies have implied that the antifungal drug fluconazole (FLC) can induce oxidative stress in C. neoformans by increasing the production of reactive oxygen species (ROS), as presence of the antioxidant ascorbic acid (AA) could reverse the inhibitory effects of FLC on C. neoformans. However, in Candida albicans, AA has been shown to stimulate the expression of genes essential for ergosterol biosynthesis. Hence, the contribution of ROS in FLC-mediated growth inhibition remains unclear. Results In order to determine whether counteracting ROS generated by FLC in C. neoformans can contribute to diminishing inhibitory effects of FLC, we tested three other antioxidants in addition to AA, namely, pyrrolidine dithiocarbamate (PDTC), retinoic acid (RA), and glutathione (GSH). Our data confirm that there is an increase in ROS in the presence of FLC in C. neoformans. Importantly, all four antioxidants reversed FLC-mediated growth inhibition of C. neoformans to various extents. We further verified the involvement of increased ROS in FLC-mediated growth inhibition by determining that ROS-scavenging proteins, metallothioneins (CMT1 and CMT2), contribute to growth recovery by PDTC and AA during treatment with FLC. Conclusion Our study suggests that ROS contributes to FLC-mediated growth inhibition and points to a complex nature of antioxidant-mediated growth rescue in the presence of FLC.

Antifungal curcumin induces reactive oxygen species and triggers an early apoptosis but prevents hyphae development by targeting the global repressor TUP1 in Candida albicans

2010 ◽  
Vol 30 (6) ◽  
pp. 391-404 ◽  
Author(s):  
Monika Sharma ◽  
Raman Manoharlal ◽  
Nidhi Puri ◽  
Rajendra Prasad
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Candida Albicans ◽  
Efflux Pump ◽  
Reactive Oxygen ◽  
Thymidine Uptake ◽  
Oxygen Species ◽  
Inhibitory Effects ◽  
Multidrug Efflux Pump ◽  
Early Apoptosis

In the present study, we have investigated the antifungal effects of a natural polyphenol, CUR (curcumin), against albicans and non-albicans species of Candida and have shown its ability to inhibit the growth of all the tested strains. The inhibitory effects of CUR were independent of the status of the multidrug efflux pump proteins belonging to either ABC transporter (ATP-binding cassette transporter) or MFS (major facilitator) superfamilies of transporters. By using a systemic murine model of infection, we established that CUR and piperine, when administered together, caused a significant fungal load reduction (1.4log10) in kidneys of Swiss mice. Additionally, CUR raised the levels of ROS (reactive oxygen species), which, as revealed by annexin V–FITC labelling, triggered early apoptosis in Candida cells. Coincident with the raised ROS levels, mRNAs of tested oxidative stress-related genes [CAP1 (Candida albicans AP-1), CaIPF7817 (putative NADH-dependent flavin oxidoreductase), SOD2 (superoxide dismutase 2), GRP2 (NADPH-dependent methyl glyoxal reductase) and CAT1 (catalase 1)] were also elevated. The growth inhibitory effects of CUR could be reversed by the addition of natural and synthetic antioxidants. Notably, independent of ROS status, polyphenol CUR prevented hyphae development in both liquid and solid hypha-inducing media by targeting the global suppressor TUP1 (thymidine uptake 1). Taken together, our results provide the first evidence that CUR acts as an antifungal agent, via generation of oxidative stress, and inhibits hyphae development by targeting TUP1.

β-Adrenergic receptor function and oxygen radical production in bovine pulmonary alveolar macrophages

1988 ◽  
Vol 66 (12) ◽  
pp. 1538-1541 ◽  
Author(s):  
P. D. Conlon ◽  
P. O. Ogunbiyi ◽  
W. D. Black ◽  
P. Eyre
Keyword(s):  
Reactive Oxygen Species ◽  
Alveolar Macrophages ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inhibitory Effects ◽  
Ici 118,551 ◽  
Oxygen Radical Production ◽  
Pulmonary Alveolar Macrophages ◽  
Species Production

Reactive oxygen species production by bovine pulmonary alveolar macrophages was evaluated by a chemiluminescence assay utilizing luminol and opsonized zymosan. Incubation with dobutamine (5 × 10−8 and 5 × 10−7 M) or isoproterenol (5 × 10−8 and 5 × 10−7 M) prior to zymosan challenge significantly (p < 0.05) increased the time for chemiluminescence to begin, and significantly decreased the level of maximum chemiluminescence. The agonists' inhibitory effects on maximum chemiluminescence were significantly reduced by pre-incubation with the appropriate antagonist (atenolol at 1 × 10−6 M for dobutamine; and propranolol at 1 × 10−6 M for isoproterenol). Salbutamol at 1 × 10−6 M significantly reduced the level of maximum chemiluminescence only, but did not increase the time for chemiluminescence to begin. This effect was significantly reduced by the presence of the β2-antagonist ICI 118,551 at 1 × 10−6 M. The results reveal the presence of (β1- and (β2-adrenoceptors on bovine pulmonary alveolar macrophages, and suggest that these receptors are important in the regulation of reactive oxygen species production by these cells.

Sign in / Sign up

Export Citation Format

Share Document