<i>p</i>-Coumaroyl Anthocyanin Mixture Isolated from Tuber Epidermis of <i>Solanum tuberosum</i> Attenuates Reactive Oxygen Species and Pro-inflammatory Mediators by Suppressing NF-κB and STAT1/3 Signaling in LPS-Induced RAW264.7 Macrophages

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.

Download Full-text

Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages

Molecular Medicine Reports ◽

10.3892/mmr.2012.1138 ◽

2012 ◽

Vol 7 (1) ◽

pp. 242-246 ◽

Cited By ~ 22

Author(s):

YI ZHONG ◽

TINGRONG LIU ◽

WENYAN LAI ◽

YING TAN ◽

DI TIAN ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Heme Oxygenase ◽

Reactive Oxygen ◽

Inhibitory Effect ◽

Heme Oxygenase 1 ◽

Oxygen Species ◽

Monocyte Chemoattractant Protein 1 ◽

Monocyte Chemoattractant ◽

Monocyte Chemoattractant Protein ◽

Raw264.7 Macrophages

Download Full-text

Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201720160836 ◽

2017 ◽

Vol 89 (1 suppl) ◽

pp. 661-674 ◽

Cited By ~ 4

Author(s):

DA HYE KWON ◽

JIN WOO JEONG ◽

EUN OK CHOI ◽

HYE WON LEE ◽

KI WON LEE ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Inflammatory Mediators ◽

Reactive Oxygen ◽

Oxygen Species ◽

Inhibitory Effects

Download Full-text

Induction of inducible nitric oxide synthase increases the production of reactive oxygen species in RAW264.7 macrophages

Bioscience Reports ◽

10.1042/bsr20090048 ◽

2010 ◽

Vol 30 (4) ◽

pp. 233-241 ◽

Cited By ~ 33

Author(s):

Kai Zhao ◽

Zhen Huang ◽

Hongling Lu ◽

Juefei Zhou ◽

Taotao Wei

Keyword(s):

Nitric Oxide ◽

Reactive Oxygen Species ◽

Nitric Oxide Synthase ◽

Reactive Oxygen ◽

Inos Expression ◽

Raw264.7 Cells ◽

Ros Production ◽

Oxygen Species ◽

Raw264.7 Macrophages ◽

Oxide Synthase

Macrophages produce a large volume of ROS (reactive oxygen species) through respiratory burst. However, the influence of iNOS [inducible NOS (nitric oxide synthase)] activation on ROS production remains unclear. In the present study, the kinetic generation of ROS in RAW264.7 murine macrophages was monitored by chemiluminescence. PMA induces a robust chemiluminescence in RAW264.7 cells, suggesting PKC (protein kinase C)-related assembly and activation of NOX (NADPH oxidase). The effects of iNOS induction on ROS production were examined. Induction of iNOS expression in RAW264.7 cells with LPS (lipopolysaccharide; 1 μg/ml) causes a significant increase in PMA-induced chemiluminescence, which could be enhanced by the NOS substrate, L-arginine, and could be abolished by the NOS inhibitor, L-NNA (NG-nitro-L-arginine). Further experiments reveal that induction of iNOS expression enhances the PMA-stimulated phosphorylation of the p47phox subunit of NOX, and promotes the relocalization of cytosolic p47phox and p67phox subunits to the membrane. Inhibition of PKCζ by its myristoylated pseudosubstrate significantly decreased the PMA-stimulated phosphorylation of the p47phox in LPS-pretreated cells, suggesting that PKCζ is involved in the iNOS-dependent assembly and activation of NOX. Taken together, the present study suggests that the induction of iNOS upregulates the PMA-induced assembly of NOX and leads to the enhanced production of ROS via a PKCζ-dependent mechanism.

Download Full-text