scholarly journals The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats

2010 ◽  
Vol 25 (5) ◽  
pp. 387-395 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Marcelo Lima Ribeiro ◽  
Alessandra Gambero ◽  
Daniel Duarte da Conceição Miranda ◽  
José Aires Pereira ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Activity Index ◽  
Neutrophil Infiltration ◽  
Histopathological Analysis ◽  
Diversion Colitis ◽  
Colon Cells ◽  
Significance Level ◽  
Colon Wall ◽  
Fecal Stream

PURPOSE: Quantify the levels of oxidative DNA damage of epithelial colon cells comparing segments with and without fecal stream. METHODS: Sixty Wistar rats were subjected to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. Animals were divided into three experimental groups that were sacrificed 6, 12 and 24 weeks after surgery. In each experimental group, five animals underwent laparotomy without intestinal deviation (sham subgroup). The diagnosis of colitis was made by histopathological analysis and the inflammatory activity index by graduated scale. The neutrophil infiltration was determined by myeloperoxidase tissue levels and the intensity of oxidative DNA damage by comet assay. The Mann-Withney and Student t test were used to compare the results among experimental subgroups and the Kruskal-Wallis test for variance analysis, adopting a significance level of 5% (p<0.05). RESULTS: Colon segments without fecal stream was shown higher histological inflammatory score of the colon wall after 12 and 24 weeks (p=0.001) that increased with the time of diversion (p=0.01). The activity of myeloperoxidase in segments without fecal stream decreased with the time (p=0.001). Oxidative DNA damage levels were significantly higher in the segments without fecal stream, (p=0.0001), independent of time of colon diversion, and increase with the time (p=0.0007). CONCLUSIONS: Colon segments without fecal stream showed high levels of oxidative DNA damage related to histological alterations observed in diversion colitis. The levels of oxidative DNA damage in segments devoid of the fecal stream increase with the time of intestinal exclusion.

scholarly journals Oxidative damage to DNA in independent-living elderly persons and their correlations with sociodemographic, anthropometric, and functional parameters

2017 ◽  
Vol 20 (2) ◽  
pp. 225-231
Author(s):  
Gilson Luis da Cunha ◽  
Greice Terezinha de Oliveira ◽  
Daiane Bolzan Berlese ◽  
Geraldine Alves dos Santos
Keyword(s):  
Dna Damage ◽  
Independent Living ◽  
Oxidative Dna Damage ◽  
Blood Collection ◽  
Elderly Persons ◽  
Fat Percentage ◽  
Cross Sectional ◽  
Significance Level ◽  
Functional Parameters ◽  
Cross Sectional Design

Abstract Objective: To evaluate the correlations between oxidative DNA damage among elderly persons aged between 60 and 79 years and sociodemographic, anthropometric and functional parameters. Method: The present study has a descriptive, quantitative and cross-sectional design. A group of 195 independent-living elderly persons of both genders underwent blood collection and the subsequent measurement of serum concentrations of 8-OHdG, a residue generated by the attack of reactive oxygen species to DNA. The same subjects also underwent evaluation for body mass index (BMI), body fat percentage, the Short Physical Performance Battery (SPPB), and the education level of the participants was analyzed. Statistical analysis was performed using the Spearman correlation test, adopting a 5% significance level. Result: Higher fat percentage and BMI are directly correlated with higher concentrations of 8-OHdG, while SPPB and education were inversely correlated with the concentration of this molecule in the sample. Conclusion: These results suggest factors such as lifestyle and educational level influenced oxidative DNA damage in these elderly persons and had an impact on their functional capacity.

Impact of lactic acid bacteria on oxidative DNA damage in human derived colon cells

2008 ◽  
Vol 46 (4) ◽  
pp. 1221-1229 ◽  
Author(s):  
Verena Juliana Koller ◽  
Brigitte Marian ◽  
Reinhard Stidl ◽  
Armen Nersesyan ◽  
Heike Winter ◽  
...  
Keyword(s):  
Dna Damage ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oxidative Dna Damage ◽  
Colon Cells

5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

2010 ◽  
Vol 56 (4) ◽  
pp. 1037-1046 ◽  
Author(s):  
Caroline Caltabiano ◽  
Felipe Rodrigues Máximo ◽  
Ana Paula Pimentel Spadari ◽  
Daniel Duarte da Conceição Miranda ◽  
Marcia Milena Pivatto Serra ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Colonic Mucosa ◽  
Aminosalicylic Acid ◽  
Fecal Stream ◽  
In Cells

scholarly journals Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

2010 ◽  
Vol 25 (3) ◽  
pp. 231-240 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Ronaldo Nonose ◽  
Ana Paula Pimentel Spadari ◽  
Felipe Rodrigues Máximo ◽  
Denise Gonçalves Priolli ◽  
...  
Keyword(s):  
Tissue Expression ◽  
Computer Assisted ◽  
Colon Mucosa ◽  
Diversion Colitis ◽  
Significance Level ◽  
Fecal Stream ◽  
Post Test ◽  
Computer Assisted Image Processing ◽  
Computer Assisted Image ◽  
Group B

PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

The effects of oxidative DNA damage and mutations in the p53 protein on cells of the colonic mucosa with and without the fecal stream: an experimental study in rats

2010 ◽  
Vol 45 (6) ◽  
pp. 714-724 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Priscila Cecília Mendes Bartocci ◽  
Carlos Vieira do Carmo ◽  
José Aires Pereira ◽  
Daniel Duarte da Conceição Miranda ◽  
...  
Keyword(s):  
Experimental Study ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Colonic Mucosa ◽  
P53 Protein ◽  
Fecal Stream

Acrylamide Exposure and Oxidative DNA Damage, Lipid Peroxidation and Fasting Plasma Glucose Alteration: Association and Mediation Analyses in Chinese Urban Adults

2020 ◽  
Author(s):  
Bin Wang ◽  
Weihong Qiu ◽  
Shijie Yang ◽  
Limin Cao ◽  
Chunmei Zhu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
Oxidative Dna Damage ◽  
Adult Population ◽  
Prostaglandin F2α ◽  
Mediation Analyses ◽  
Mediating Role ◽  
Fasting Plasma

<a><b>OBJECTIVE: </b></a>Acrylamide exposure from daily-consumed food has raised global concern.<b> </b>We aimed to assess the exposure-response relationships of internal acrylamide exposure with oxidative DNA damage, lipid peroxidation and fasting plasma glucose (FPG) alteration, and investigate the mediating role of oxidative DNA damage and lipid peroxidation in the association of internal acrylamide exposure with FPG. <p><b>RESEARCH DESIGN AND METHODS:</b> FPG and urinary biomarkers of oxidative DNA damage (8-hydroxy-deoxy-guanosine, 8-OHdG), lipid peroxidation (8-iso-prostaglandin-F2α, 8-iso-PGF2α) and acrylamide exposure (N-acetyl-S-(2-carbamoylethyl)-L-cysteine, AAMA; N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine, GAMA) were measured for 3,270 general adults from the Wuhan-Zhuhai cohort. The associations of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG were assessed by linear mixed models. The mediating roles of 8-OHdG and 8-iso-PGF2α were evaluated by mediation analysis.</p> <p><b>RESULTS:</b> We found significant linear positive dose-response relationships of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG (except GAMA with FPG), and 8-iso-PGF2α with FPG. Each 1-unit increase in log-transformed level of AAMA, ΣUAAM (AAMA+GAMA) or 8-iso-PGF2α was associated with a 0.17-, 0.15- or 0.23-mmol/L increase in FPG, respectively (<i>P </i>or/and<i> P trend</i><0.05). Each 1% increase in AAMA, GAMA or ΣUAAM was associated with a 0.19%, 0.27% or 0.22% increase in 8-OHdG, respectively, and a 0.40%, 0.48% or 0.44% increase in 8-iso-PGF2α, respectively (<i>P </i>and<i> P trend</i><0.05). Increased 8-iso-PGF2α rather than 8-OHdG significantly mediated 64.29% and 76.92% of the AAMA and ΣUAAM associated-FPG increases, respectively.</p> <p><b>CONCLUSIONS:</b> Exposure of general adult population to acrylamide was associated with FPG elevation, oxidative DNA damage and lipid peroxidation, which in turn partly mediated acrylamide-associated FPG elevation.<b></b></p>

Serum of 8-OHdG as a potential biomarker of oxidative DNA damage in workers exposed to harmful working environments

2020 ◽  
pp. 783-783
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Blood Serum ◽  
Oxidative Dna Damage ◽  
Working Environment ◽  
Working Environments ◽  
Potential Biomarker ◽  
High Level ◽  
Damage Marker

In the blood serum of 70% individuals exposed to harmful factors of the working environment, a high level of oxidative stress and the DNA damage marker 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) were detected.

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