scholarly journals Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 774
Author(s):  
Nastassja Kimberlly Lima ◽  
Eloisa Schneider Da Silva ◽  
Rayane Monique Sete Da Cruz ◽  
Pedro Henrique Riboldi Monteiro ◽  
Glacy Jaqueline Da Silva
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Aerial Part ◽  
Large Scale ◽  
Dry Mass ◽  
Fresh Mass ◽  
Limiting Factor ◽  
Naphthalene Acetic Acid ◽  
In Vitro Cultivation

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

scholarly journals Desenvolvimento in vitro da orquídea ameaça de extinção Laelia marginata Lindl. em meios de cultivo contendo diferentes relações nitrato/amônio

2017 ◽  
Vol 23 (4) ◽  
pp. 392-399
Author(s):  
Douglas Junior Bertoncelli ◽  
Guilherme Augusto Cito Alves ◽  
Rodrigo Thibes Hoshino ◽  
Gustavo Henrique Freiria ◽  
Felipe Favoretto Furlan ◽  
...  
Keyword(s):  
Root Length ◽  
Aerial Part ◽  
Culture Media ◽  
Dry Mass ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Initial Development ◽  
Randomized Design ◽  
Vitro Development

In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3 - and NH4 + : T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.

scholarly journals Purple nutsedge (Cyperus rotundus L.) extract as carrot growth and production inducer

2021 ◽  
Vol 17 (5) ◽  
pp. 31-42
Author(s):  
Geraldo Pastore ◽  
César Antônio da Silva ◽  
Nelson Geraldo Golinski ◽  
Fernando Zuchello ◽  
Cícero José da Silva
Keyword(s):  
Aqueous Extract ◽  
Root Length ◽  
Aerial Part ◽  
Dry Mass ◽  
Root Diameter ◽  
Cyperus Rotundus ◽  
Fresh Mass ◽  
Root Mass ◽  
Purple Nutsedge ◽  
Total Productivity

There is evidence that the purple nutsedge (Cyperus rotundus L.) aqueous extract can induce the plants rooting due to the high auxin concentrations. This study aimed to evaluate the effect of application seasons and concentrations of purple nutsedge aqueous extract application on the growth and production of carrot, cultivar Brasília. The experimental design was in randomized blocks, in a 2 x 5 factorial scheme in two seasons of the extract application (25 and 50 days after sowing) and five concentrations of (0%, 10%, 20%, 30% and 40%) with four replicates. Harvesting was carried out at 90 days after sowing, being evaluated the following morphological parameters: root diameter (cm); root fresh mass (g plant-1); plant height (cm); aerial part fresh mass (g plant-1); root dry mass (g plant-1); aerial part dry mass (g plant-1); plant dry mass (g plant-1); ratio between root dry mass and aerial part dry mass; root length (cm); commercial productivity (t ha-1); defective root mass (t ha-1); total productivity (t ha-1); commercial yield (roots kg-1); and root classification by length. The extract application at 50th day was more efficient for the root diameter (3.37 cm); fresh root mass (120.7 g plant-1); root length (15.4 cm); commercial productivity (68.2 t ha-1); total productivity (73.6 t ha-1); and commercial roots (8.8 roots kg-1). Root classification by length showed a higher percentage in the 14 cm classification range. The parameters did not present significant differences in both seasons, at 25th and 50th days, respectively, for root dry mass (12.0 and 12.8 g plant-1), ratio between dry mass of roots and aerial part (1.66 and 1.57), and defective root mass (1.3 and 1.5 t ha-1).

scholarly journals Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

2010 ◽  
Vol 34 (6) ◽  
pp. 1439-1445 ◽  
Author(s):  
Lenaldo Muniz de Oliveira ◽  
Renato Paiva ◽  
José Raniere Ferreira de Santana ◽  
Flávia Dionísio Pereira ◽  
Raírys Cravo Nogueira ◽  
...  
Keyword(s):  
Large Scale ◽  
Dry Mass ◽  
Tropical Species ◽  
In Vitro Cultivation ◽  
Growth And Survival ◽  
Agronomic Potential ◽  
Bud Growth ◽  
Annona Glabra ◽  
Mineral Accumulation

Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ), kinetin (KIN), and zeatin (ZEA). KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals Hydroponics growth of Eucalyptus saligna Sm. on salt-stress mediated by sodium chloride

2012 ◽  
Vol 3 (4) ◽  
pp. 213-218 ◽  
Author(s):  
André Luís Lopes da Silva ◽  
Yohana de Oliveira ◽  
Roberson Dibax ◽  
Jefferson da Luz Costa ◽  
Gessiel Newton Scheidt ◽  
...  
Keyword(s):  
Salt Stress ◽  
Aerial Part ◽  
Dry Mass ◽  
Hydroponic Culture ◽  
Fresh Mass ◽  
Root Number ◽  
Eucalyptus Saligna ◽  
Volume Number ◽  
Micropropagated Plants ◽  
Fresh And Dry Mass

The aim of this work was to evaluate the growth of two clones of Eucalyptus saligna on salt-stress mediated by NaCl in hydroponics. Micropropagated plants of the clones p0 and p1 were acclimatizated and cultivated in hydroponics at 0 and 300 mM NaCl levels. The total length, volume, number, fresh mass and dry mass of the roots, the height, fresh and dry mass of the aerial part and the fresh  and dry mass of the complete plant were evaluated to the 14 days of hydroponic culture with NaCl. There were significant differences among the clones. The clone p0 was superior to the clone p1 in relation to volume of the roots, root number, root fresh mass and total fresh mass of the root. Regards the effect of the salinity on the plants, significant reduction was observed in the height of the aerial part, fresh mass of the aerial part and the total fresh mass. Even so, the interaction between the clones and the concentrations of NaCl was significant for the total fresh mass. In the period of 14 days of hydroponic culture on 300 mM NaCl was possible to discriminate these two clones in relation to the tolerance and susceptibility to the salt stress. The clone p0 presented higher growth and larger tolerance to the salinity than clone p1.

scholarly journals In-vitro development of Nauclea diderrichii (de Willd. & Th. Dur) Merrin liquid-M Smedia supplemented with benzyl amino purine (BAP) and naphthalene acetic acid (NAA)

2021 ◽  
Vol 24 (12) ◽  
pp. 2065-2069
Author(s):  
J.O. Afolabi ◽  
R.I. Oyediran ◽  
Y.O. Aguda ◽  
E.A. Adekunle
Keyword(s):  
Growth Regulators ◽  
Adventitious Shoots ◽  
Transition Process ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Temporary Immersion Bioreactor ◽  
Group A ◽  
Bioreactor System ◽  
Vitro Development

The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process. The response of N. diderrichii explants was assessed in liquid-M Smedia with a view to mass produce its seedlings using TIBs. Seven treatments consisting (A) 0.0/0.0, (B) 0.0/0.1, (C) 0.1/0.0, (D) 0.2/0.1, (E) 0.3/0.2, (F) 0.4/0.3 and (G) 0.5/0.4mg/lBAP/NAA combinations were studied. Each group consist of seven replicates and group A without Growth Regulators (GR) serves as control. The results at 4 Weeks after Inoculation (WAI) showed that effects of the growth regulators were significant on shoot length and number of adventitious shoots while number of roots and leaves were closely related. Treatment E produced highest number of adventitious shoots (3.6) which was higher than 0.9 shoots from treatment G and closely related to others. Maximum number of leaves (16.6) was produced by treatment F followed by E (15.7) while the least (12) was obtained in treatment A. The highest number of roots (4.9) was obtained from treatments B, followed by E (4.3) with the lowest being recorded in C (2.43). Liquid MS medium supplemented with 0.3/0.2mg/lBAP/NAA shows some promise for plantlets generation for the purpose of multiple shoot production of N. diderrichii in TIBs.

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

scholarly journals Effect of growth hormone and growth media on the rooting and shooting of Zanthoxylum armatum stem cuttings

2018 ◽  
Vol 28 (2) ◽  
pp. 3-12
Author(s):  
N. Phuyal ◽  
P. K. Jha ◽  
P. P. Raturi ◽  
S. Gurung ◽  
S. Rajbhandary
Keyword(s):  
Root Length ◽  
Large Scale ◽  
Growth Media ◽  
Naphthalene Acetic Acid ◽  
Scale Production ◽  
Stem Cuttings ◽  
Efficient Treatment ◽  
Zanthoxylum Armatum ◽  
Significant Difference ◽  
Rooting Media

The common method of propagation is through seeds but seed germination in Zanthoxylum armatum is very low due to the presence of hard seed coat, which might be a great hurdle for large scale production of plantlets. So an attempt was made in this study to see the effect of different growth hormones, their concentrations and different rooting media on the rooting and sprouting of Z. armatum. The stem cuttings of Z. armatum were treated with two types of auxins namely Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) at different concentrations (2000 ppm, 3000 ppm and 5000 ppm), while the untreated cuttings were used as control. The cuttings were planted in three different rooting media: sand, neopeat and mix (containing a mixture of sand, soil and vermin-compost). The completely randomized design was used for the experiment. The total number of stem cuttings of Z. armatum used in the experiment was 1080 for 18 treatments in three replicates (20 cuttings per treatment x 18 treatments x 3 replicates). The experiment was set up in controlled greenhouse conditions at Dabur Nepal Private Limited Nursery, Banepa, Kavre District. The parameters evaluated were root length, shoot length and number of roots per cutting. The collected data were analyzed statistically using R-program with Agricola. Least significant difference (LSD) and Duncan multiple Range Test (DMRT), as mean separation technique was applied to identify the most efficient treatment in the rooting and shooting behavior of Z. armatum (Gomez and Gomez, 1984). Hormone concentration and growth media significantly affected the rooting and shooting ability of Z. armatum stem cuttings. IBA was found to be more effective than NAA. Neopeat medium was better than sand and mix media. The highest number of roots (6.5) and root length (11.6 cm) were recorded under IBA 5000 ppm in neopeat medium.

scholarly journals SPATIAL DISTRIBUTION OF PHYSIOLOGICAL QUALITY OF Joannesia princeps Vell. SEEDS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Rômulo André Beltrame ◽  
José Carlos Lopes ◽  
Julião Soares de Souza Lima ◽  
Vagner Mauri Quinto
Keyword(s):  
Spatial Distribution ◽  
Root System ◽  
Spatial Dependence ◽  
Aerial Part ◽  
Dry Mass ◽  
Fresh Mass ◽  
Plastic Bags ◽  
Mother Plants ◽  
Physiological Quality

ABSTRACT The objective of this study was to analyze the spatial distribution of the attributes that determine the physiological quality of Joannesia princeps seeds. Seeds were collected, individually from, 40 mother plants in the Horto Florestal Municipal Laerth Paiva Gama, in Alegre-ES, which constituted the georeferenced sampling mesh. For germination, the seeds tegument was cracked and was held the imbibition in gibberellic acid GA3 500 mg L-1 for 24 h in environmental chamber with temperature adjusted to 30 ºC. The seeds were sown in plastic bags of ± 600 cm3 containing soil+sand+manure in the proportion of 1:1:1. On the 65th day after sowing, the following variables were analised: emergence (E), emergence speed index (IVE), root length (CR), diameter of base (DC), shoot length of the aerial part (CPA), fresh mass of the root system (MFSR), fresh mass of the aerial part (MFPA), dry mass of the root system (MSSR) and dry mass of the aerial part (MSPA). Data were subjected to descriptive statistics, geostatistics and kriging. With the exception of CPA and MFSR, it was found spatial dependence for the other studied variables: E, IVE, CR, DC, MFPA, MSSR e MSPA; varying of 14,0 a 47,5 m, so that the lowest range for the CR and longer range and degree of spatial dependence for the IVE. The analysis of the spatial distribution of the physiological quality of seeds of J. princeps can be used as a tool to define the sampling region of seeds with high and low vigor.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

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