Steroidal saponins from the roots of Solanum sisymbriifolium Lam. (Solanaceae) have inhibitory activity against dengue virus and yellow fever virus

ABSTRACT Two yellow fever virus (YFV)/dengue virus chimeras which encode the prM and E proteins of either dengue virus serotype 2 (dengue-2 virus) or dengue-4 virus within the genome of the YFV 17D strain (YF5.2iv infectious clone) were constructed and characterized for their properties in cell culture and as experimental vaccines in mice. The prM and E proteins appeared to be properly processed and glycosylated, and in plaque reduction neutralization tests and other assays of antigenic specificity, the E proteins exhibited profiles which resembled those of the homologous dengue virus serotypes. Both chimeric viruses replicated in cell lines of vertebrate and mosquito origin to levels comparable to those of homologous dengue viruses but less efficiently than the YF5.2iv parent. YFV/dengue-4 virus, but not YFV/dengue-2 virus, was neurovirulent for 3-week-old mice by intracerebral inoculation; however, both viruses were attenuated when administered by the intraperitoneal route in mice of that age. Single-dose inoculation of either chimeric virus at a dose of 105 PFU by the intraperitoneal route induced detectable levels of neutralizing antibodies against the homologous dengue virus strains. Mice which had been immunized in this manner were fully protected from challenge with homologous neurovirulent dengue viruses by intracerebral inoculation compared to unimmunized mice. Protection was associated with significant increases in geometric mean titers of neutralizing antibody compared to those for unimmunized mice. These data indicate that YFV/dengue virus chimeras elicit antibodies which represent protective memory responses in the mouse model of dengue encephalitis. The levels of neurovirulence and immunogenicity of the chimeric viruses in mice correlate with the degree of adaptation of the dengue virus strain to mice. This study supports ongoing investigations concerning the use of this technology for development of a live attenuated viral vaccine against dengue viruses.

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Fever versus fever: The role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus

Infection Genetics and Evolution ◽

10.1016/j.meegid.2013.03.008 ◽

2013 ◽

Vol 19 ◽

pp. 292-311 ◽

Cited By ~ 98

Author(s):

Kathryn A. Hanley ◽

Thomas P. Monath ◽

Scott C. Weaver ◽

Shannan L. Rossi ◽

Rebecca L. Richman ◽

...

Keyword(s):

Dengue Virus ◽

Interspecific Competition ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus

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High Fidelity of Yellow Fever Virus RNA Polymerase

Journal of Virology ◽

10.1128/jvi.78.2.1032-1038.2004 ◽

2004 ◽

Vol 78 (2) ◽

pp. 1032-1038 ◽

Cited By ~ 82

Author(s):

Konstantin V. Pugachev ◽

Farshad Guirakhoo ◽

Simeon W. Ocran ◽

Fred Mitchell ◽

Megan Parsons ◽

...

Keyword(s):

Dengue Virus ◽

Rna Polymerase ◽

Yellow Fever ◽

Error Rate ◽

Yellow Fever Virus ◽

Protein A ◽

Fever Virus ◽

Genome Replication ◽

Virus Rna

ABSTRACT Three consecutive plaque purifications of four chimeric yellow fever virus-dengue virus (ChimeriVax-DEN) vaccine candidates against dengue virus types 1 to 4 were performed. The genome of each candidate was sequenced by the consensus approach after plaque purification and additional passages in cell culture. Our data suggest that the nucleotide sequence error rate for SP6 RNA polymerase used in the in vitro transcription step to initiate virus replication was as high as 1.34 × 10−4 per copied nucleotide and that the error rate of the yellow fever virus RNA polymerase employed by the chimeras for genome replication in infected cells was as low as 1.9 × 10−7 to 2.3 × 10−7. Clustering of beneficial mutations that accumulated after multiple virus passages suggests that the N-terminal part of the prM protein, a specific site in the middle of the E protein, and the NS4B protein may be essential for nucleocapsid-envelope interaction during flavivirus assembly.

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Dengue Virus and Yellow Fever Virus Damage the Liver: A Systematic Review About the Histopathological Profiles

Journal of Gastroenterology and Hepatology Research ◽

10.17554/j.issn.2224-3992.2019.07.823 ◽

2019 ◽

Vol 8 (2) ◽

pp. 2864-2870 ◽

Cited By ~ 1

Author(s):

Dacylla Sampaio Costa ◽

◽

Lucas Arruda Moita ◽

Even Herlany Pereira Alves ◽

Ana Clara Silva Sales ◽

...

Keyword(s):

Systematic Review ◽

Dengue Virus ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus

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Yellow Fever Virus Exhibits Slower Evolutionary Dynamics than Dengue Virus

Journal of Virology ◽

10.1128/jvi.01738-09 ◽

2009 ◽

Vol 84 (2) ◽

pp. 765-772 ◽

Cited By ~ 46

Author(s):

Amadou A. Sall ◽

Ousmane Faye ◽

Mawlouth Diallo ◽

Cadhla Firth ◽

Andrew Kitchen ◽

...

Keyword(s):

Dengue Virus ◽

Yellow Fever ◽

Central African Republic ◽

Evolutionary Dynamics ◽

Nucleotide Substitution ◽

Viral Infections ◽

Yellow Fever Virus ◽

Fever Virus ◽

Limited Information ◽

Reduced Rate

ABSTRACT Although yellow fever has historically been one of the most important viral infections of humans, relatively little is known about the evolutionary processes that shape its genetic diversity. Similarly, there is limited information on the molecular epidemiology of yellow fever virus (YFV) in Africa even though it most likely first emerged on this continent. Through an analysis of complete E gene sequences, including a newly acquired viral collection from Central and West Africa (Senegal, Cameroon, Central African Republic, Côte d'Ivoire, Mali, and Mauritania), we show that YFV exhibits markedly lower rates of evolutionary change than dengue virus, despite numerous biological similarities between these two viruses. From this observation, along with a lack of clock-like evolutionary behavior in YFV, we suggest that vertical transmission, itself characterized by lower replication rates, may play an important role in the evolution of YFV in its enzootic setting. Despite a reduced rate of nucleotide substitution, phylogenetic patterns and estimates of times to common ancestry in YFV still accord well with the dual histories of colonialism and the slave trade, with areas of sylvatic transmission (such as Kedougou, Senegal) acting as enzootic/epidemic foci.

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