scholarly journals A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

2021 ◽  
Vol 54 (9) ◽  
Author(s):  
T.F.G. Souza ◽  
T.M. Pierdoná ◽  
F.S. Macedo ◽  
P.E.A. Aquino ◽  
G.F.P. Rangel ◽  
...  
Keyword(s):  
Burn Wound ◽  
Methanol Fraction ◽  
Healing Effect ◽  
Wound Model ◽  
Human Keratinocyte

scholarly journals Human keratinocyte (HaCaT) stimulation and healing effect of the methanol fraction from the decoction from leaf from Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn on experimental burn wound model

2020 ◽  
Author(s):  
Tamiris de Fátima Goebel de Souza ◽  
Taiana Magalhães Pierdoná ◽  
Fernanda Soares Macedo ◽  
Pedro Everson Alexandre de Aquino ◽  
Gisele de Fátima Pinheiro Rangel ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Human Keratinocytes ◽  
Northeastern Brazil ◽  
Easy Access ◽  
Hacat Cells ◽  
Burn Wound ◽  
Methanol Fraction ◽  
Wound Model ◽  
Human Keratinocyte ◽  
Experimental Burn

AbstractThe larger number of plants, with therapeutic potential, popularly used in Northeastern Brazil is due to their easy access and the great Brazilian biodiversity. Previously, was demonstrated that the methanol fraction from Sideroxylon obtusifolium (MFSOL) promoted an anti-inflammatory and healing activity in excisional wounds. Thus, this work aimed to investigate the healing effects of MFSOL on human keratinocytes cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to investigate migration and proliferation of cell rates. Female Swiss mice were subjected to second-degree superficial burn protocol and divided into four treatment groups: Vehicle (cream-base), 1.0% Silver Sulfadiazine (Sulfa), and 0.5% or 1.0% MFSOL cream (CrMFSOL). Samples were collected for quantification of the inflammatory mediators and histological analyses after 3, 7 and 14 days on evaluation. As result, MFSOL (50 μg/ml) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, CrMFSOL 0.5% attenuated myeloperoxidase (MPO) activity and also stimulated the release of IL-1β and IL-10, after 3 days with treatment. CrMFSOL 0.5% enhanced wound contraction, promoted tissue remodeling improvement and highest collagen production after 7 days, and VEGF release after 14 days. Therefore, MFSOL evidenced the stimulation of human keratinocyte (HaCaT) cells and improvements on wound healing via inflammatory modulation on burn injuries.

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

scholarly journals Migration of labeled bone marrow MSCs and skin fibroblasts after systemic and local transplantation in rat burn wound model

2015 ◽  
Vol 31 (5) ◽  
pp. 387-394
Author(s):  
E. A. Shchegelskaya ◽  
T. G. Grigorieva ◽  
E. A. Omelchenko ◽  
A. S. Zabirnyk ◽  
E. V. Markelova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Skin Fibroblasts ◽  
Bone Marrow Mscs ◽  
Burn Wound ◽  
Wound Model

Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model

Burns ◽  
2014 ◽  
Vol 40 (3) ◽  
pp. 511-519 ◽  
Author(s):  
Hiroshi Ito ◽  
Sven Asmussen ◽  
Daniel L. Traber ◽  
Robert A. Cox ◽  
Hal K. Hawkins ◽  
...  
Keyword(s):  
Seed Oil ◽  
Sea Buckthorn ◽  
Hippophae Rhamnoides ◽  
Burn Wound ◽  
Hippophaë Rhamnoides ◽  
Wound Model ◽  
Hippophae Rhamnoïdes L

scholarly journals An Invertebrate Burn Wound Model That Recapitulates the Hallmarks of Burn Trauma and Infection Seen in Mammalian Models

2020 ◽  
Vol 11 ◽  
Author(s):  
Evgenia Maslova ◽  
Yejiao Shi ◽  
Folke Sjöberg ◽  
Helena S. Azevedo ◽  
David W. Wareham ◽  
...  
Keyword(s):  
Burn Wound ◽  
Burn Trauma ◽  
Wound Model

535 Porcine Infected Partial-Thickness Burn Wound Model

2018 ◽  
Vol 39 (suppl_1) ◽  
pp. S241-S241
Author(s):  
F M Egro ◽  
M Schusterman ◽  
D Kim ◽  
D Grybowski ◽  
I James ◽  
...  
Keyword(s):  
Burn Wound ◽  
Wound Model ◽  
Partial Thickness ◽  
Partial Thickness Burn

scholarly journals Nerve reinnervation and itch behavior in a rat burn wound model

2018 ◽  
Vol 26 (1) ◽  
pp. 16-26
Author(s):  
Tiam M. Saffari ◽  
Barthold N. Schüttenhelm ◽  
Johan. W. van Neck ◽  
Jan C. Holstege
Keyword(s):  
Burn Wound ◽  
Wound Model

scholarly journals Staphylococcus aureus biofilms release leukocidins to elicit extracellular trap formation and evade neutrophil-mediated killing

2018 ◽  
Vol 115 (28) ◽  
pp. 7416-7421 ◽  
Author(s):  
Mohini Bhattacharya ◽  
Evelien T. M. Berends ◽  
Rita Chan ◽  
Elizabeth Schwab ◽  
Sashwati Roy ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
White Blood Cells ◽  
Neutrophil Extracellular Trap ◽  
Bacterial Survival ◽  
Chronic Infections ◽  
Burn Wound ◽  
Wound Model ◽  
Immune Defenses ◽  
Extracellular Trap

Bacterial biofilms efficiently evade immune defenses, greatly complicating the prognosis of chronic infections. How methicillin-resistant Staphylococcus aureus (MRSA) biofilms evade host immune defenses is largely unknown. This study describes some of the major mechanisms required for S. aureus biofilms to evade the innate immune response and provides evidence of key virulence factors required for survival and persistence of bacteria during chronic infections. Neutrophils are the most abundant white blood cells in circulation, playing crucial roles in the control and elimination of bacterial pathogens. Specifically, here we show that, unlike single-celled populations, S. aureus biofilms rapidly skew neutrophils toward neutrophil extracellular trap (NET) formation through the combined activity of leukocidins Panton–Valentine leukocidin and γ-hemolysin AB. By eliciting this response, S. aureus was able to persist, as the antimicrobial activity of released NETs was ineffective at clearing biofilm bacteria. Indeed, these studies suggest that NETs could inadvertently potentiate biofilm infections. Last, chronic infection in a porcine burn wound model clearly demonstrated that leukocidins are required for “NETosis” and facilitate bacterial survival in vivo.

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