scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

scholarly journals Determination of wound healing activity of Shark liver oil using the excision wound model in Wistar rats

2020 ◽  
Vol 11 (4) ◽  
pp. 7301-7308
Author(s):  
Auti S A ◽  
Jat R K ◽  
Das S K ◽  
Shukla S T
Keyword(s):  
Wound Healing ◽  
Disease Control ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Wound Contraction ◽  
Standard Drug ◽  
Wound Area ◽  
Excision Wound ◽  
Shark Liver Oil ◽  
Wound Model

There are various promising preclinical models, such as in mice, wistar rats, rabbits and pigs, which can be utilized to initiate acute or chronic wounds. These can be persuaded by many distinctive techniques, with excision the most common. After determining a proper model for a study, investigators need to choose an appropriate and reproducible technique that will permit the monitoring of the wound improvement over time. In this study, the healing power of Shark Liver Oil Emulgel (SLO) in Wistar rats were analyzed by using the excision wound model. The shark liver oil was prepared as emulgel at a concentration of 5%,10% and 15%, respectively and both Standard drug(Povidone-Iodine Ointment USP 5% w/w-PI) and SLO is applied at a concentration of 1mg/mm2; Topically. The parameters integrated for the assessment of the effects of SLO were Relative body weight changes, wound area contraction in mm2, relative wound percentage and epithelialization time. Wound area contraction was measured on 1st, 3rd, 6th, 9th, 12th, 15th, 18th and 21st day. Wistar rats treated with SLO showed substantial variations in epithelialization period and improved wound contraction in the excision wound treatment as compared to disease control. The biochemical biomarkers like SOD(Superoxide dismutases), GSH(Reduced Glutathione)and LPO(Lipid peroxidase) in the treated group have shown a significant change in the improvement of wound healing. Histopathological studies and microscopic observations specify that the topical use of Shark liver oil Emulgel extensively improved wound contraction, collagenation and epithelialization with well-organized dermis devoid of inflammatory cells in contrast to disease control.

EVALUATION OF WOUND HEALING ACTIVITY OF SILVER SULFADIAZINE EMULGEL (1%) IN THE RAT BURN WOUND MODEL AND ITS SKIN IRRITATION STUDY

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (11) ◽  
pp. 40-43
Author(s):  
P. J. Patil ◽  
◽  
S. P Chaudhari . ◽  
S. V. Ghodekar
Keyword(s):  
Wound Healing ◽  
Skin Irritation ◽  
Silver Sulfadiazine ◽  
Burned Area ◽  
Topical Formulation ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Wound Model ◽  
Skin Irritation Test

The study provides a scientific evaluation of the biological and therapeutic properties of new topical formulation of silver sulfadiazine emulgel (1%) as an alternative for the treatment of burn wounds. The solid dispersion was prepared with poloxomer 407 by melt method and is used for emulgel formulation. The prepared silver sulfadiazine emulgel (1%) was compared with marketed silver sulfadiazine creamon healing of burn wounds in rats. Burned area evaluations on the 4th, 8th, 12th and 16th days showed statistically significant better burn wound healing in silver sulfadiazine emulgel (1%) as compared to marketed silver sulfadiazine (1%) group. Moreover, it showed no irritation when tested in rabbit skin irritation test. In conclusion, application of silver sulfadiazine emulgel may be more effective in healing burn related skin wounds in the rat model.

scholarly journals Preparation and evaluation of burns wound healing ointment base of leaves and stem bark of Anthocleista djalonensis (L) extract using animal model.

2020 ◽  
Vol 1 (02) ◽  
pp. 25-32
Author(s):  
Chinenye Nnenna Ugwu ◽  
Ezinwanne Nneoma Ezeibe ◽  
Chinekwu Sherridan Nwobi ◽  
Chinelo Charity Eze ◽  
Somtochukwu Amuche Evurani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Bark ◽  
Positive Control ◽  
Silver Sulfadiazine ◽  
Wound Contraction ◽  
Bark Extract ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wounds ◽  
Ointment Base

BACKGROUND: Burn wounds are inescapable life events especially in low income areas. Contamination of the burn wound site results in localized wound infection, hence the need for potent phytochemicals readily available for wound healing. The use of stem bark of Anthocleista djalonensis efficacy for burn wound has not been evaluated to the best of our knowledge. AIM: The study is aimed at evaluating and comparing the wound healing potential of ointment base of leaves and Stem bark of Anthocleista djalonensis on burn wounds created on wistar albino rats. METHODS: The leaves and Stem bark of Anthocleista djalonensis collected were extracted using 95%v/v methanol and phytochemical analysis conducted. Simple ointments of varying concentrations were formulated to screen for wound healing activity using the burn wound model on experimental rats grouped into six (n=4). Group 1 was treated with silver sulfadiazine cream (positive control), group 2 with ointment base (negative control), group 3 with 1% stem bark extract ointment, group 4 with 2% stem bark ointment, group 5 with 1% leave extract ointment, and group 6 with 2% leave extract ointment.  All animals were anesthetized before the creation of burn wounds. Measurement was taken on day zero and the wound was left untreated for 48 hours in order to allow bacterial colonization before daily treatment of the wound for 16 days. RESULTS: The result of the phytochemical screening revealed that both extract of Anthocleista djalonensis contains flavonoids, tannins and saponins. On day 2, 1% stem bark, 2% stem bark and 1% leaf extract had 16%, 15%, and 10% wound contraction respectively which was higher than the 8.5% wound contraction of silver sulfadiazine. Also as the concentration of the extract increased, the wound healing effect also increased as seen by the percentage wound contraction on day 16 for all treatment.  CONCLUSION: The findings of the study have shown that methanolic extracts of stem bark and leaf of Anthocleista djalonensis contained bioactive constituents which have burn wound healing activity. The stem bark extract showed better activity when compared with the leaf extract and also the positive control (silver sulfadiazine).    

WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (03) ◽  
pp. 40-46
Author(s):  
M. C. Divakar ◽  
◽  
S Lakshmi Devi
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Excision Wound ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Wrightia Tinctoria ◽  
Incision Wound ◽  
Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

scholarly journals Topical treatment of standardised burns with herbal remedies in model rats

2005 ◽  
Vol 5 (4) ◽  
pp. 50-57 ◽  
Author(s):  
Fahir Bečić ◽  
Nedžad Mulabegović ◽  
Zakira Mornjaković ◽  
Elvedina Kapić ◽  
Senad Prašović ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Herbal Remedies ◽  
Control Group ◽  
Therapeutic Effects ◽  
Skin Area ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

scholarly journals MANFAAT EKSTRAK ETANOL DAUN REMEK DAGING (Hemigraphis colorata W. Bull) TERHADAP LUKA BAKAR PADA TIKUS

2020 ◽  
Vol 13 (1) ◽  
pp. 32-41
Author(s):  
Lusi Putri Dwita ◽  
Vera Ladeska ◽  
Aisyah Ramadhani ◽  
Dwi Rahma Augusta ◽  
Retno Tri Saufia
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Burn Wound ◽  
Burn Wounds ◽  
Wound Induction ◽  
Collagen Density

ABSTRACT Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5%  remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%. Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen.  ABSTRAK Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%. Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.  

scholarly journals The Effects of Topical Vitamin C on Burn Wound Healing

2021 ◽  
Vol 24 (2) ◽  
pp. 204-215
Author(s):  
Mohammad Haddadi ◽  
◽  
Davood Movahedzadeh ◽  
Elaheh Jaghouri ◽  
Hamid Robat Sarpooshi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Vitamin C ◽  
Topical Application ◽  
Repeated Measures ◽  
Assessment Tool ◽  
Intervention Group ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wounds

Background and Aim: Topical supplements and vitamins have often been used to accelerate the healing of burn wounds. This study investigated the effects of topical application of vitamin C solution on burn wounds. Methods & Materials: This research was performed on 30 patients with extensive second-degree burns on one or two organs with similar burns. The study subjects were randomly divided into the intervention and control groups. The intervention group received topical application of vitamin C solution in addition to silver sulfadiazine ointment in the burn area; however, only silver sulfadiazine ointment was applied in the burn area in the control group. The wounds were examined and scored on days 1, 3, 7, and 14 using the Bates-Jensen Wound Assessment Tool. The collected data were analyzed in SPSS using repeated-measures Analysis of Variance (ANOVA). Ethical Considerations: This study was approved by the ethics committee of sabzevar university medical sciences. (Code: IR.MEDSAB.REC.1394.60). Results Eighteen of the 30 examined patients were male and 12 were female. Their Mean±SD age and body mass index were 43.03±11.90 years and 27.82±4.08 kg/m2, respectively. Conclusion: The repeated-measures ANOVA data indicated that the study groups were significantly different concerning the final mean scores given to the wounds (P=0.047). In other words, the research groups significantly differed in wound healing; thus, topical application of vitamin C significantly improved wound healing in the study participants.

scholarly journals Determination of Wound Healing Activity of Shark Liver Oil using Burn Wound Model in Wistar Rats

2021 ◽  
Vol 13 (2) ◽  
pp. 113-117
Author(s):  
Samiullah Allahbaksh Auti ◽  
Rakesh Kumar Jat ◽  
Supriya Kumar Das ◽  
Shukla ST Shukla
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Burn Wound ◽  
Shark Liver Oil ◽  
Wound Model ◽  
Wound Healing Activity

scholarly journals Effects of Topical Antimicrobial Formulations on Pseudomonas aeruginosa Biofilm in an In vivo Porcine Burn Wound Model

2019 ◽  
Author(s):  
S.C. Davis ◽  
M. Solis ◽  
J. Gil ◽  
J. Valdes ◽  
A. Higa ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Healing ◽  
Post Infection ◽  
Wound Dressings ◽  
Wound Infections ◽  
Burn Wound ◽  
Bacterial Counts ◽  
Burn Wounds ◽  
Wound Model

AbstractSilver has been incorporated into a variety of wound dressings and topical agents to prevent and combat wound infections. Pseudomonas aeruginosa is a common cause of burn wound infections and well-known biofilm producer. The objective of this study was to evaluate the effects of a panel of wound dressings containing different silver formulations on P. aeruginosa biofilms using an in vivo porcine burn wound model. Second-degree burns were created on the skin of specific pathogen-free pigs (n = 3) and inoculated with 2.14 × 105 cfu P. aeruginosa per wound. Biofilms were allowed to develop for 24 h, and then each wound was treated with one of 6 treatments: silver oxynitrate dressing (OXY), silver oxynitrate powder (POWD), nanocrystalline silver dressing (NANO), silver chloride dressing (AGCL), silver sulfadiazine (SSD), or a negative control polyurethane film with no silver-based formulation (NEG). Wounds were cultured at D3 post-infection (n = 3 per pig per treatment) and at D6 post-infection (n = 3 per pig per treatment) for quantification of bacteria. On D6, biopsies (n = 3 per treatment) were taken from POWD, SSD, and NEG wounds and wound healing progress was evaluated histologically. At the time of treatment initiation, 24 h post-infection, 8.71 log cfu P. aeruginosa were present in burn wounds. On D3 and D6, all treatments significantly reduced bacterial counts in wounds as compared to NEG, but POWD caused an approximately 7-log reduction in bacterial counts on both days and was the only treatment to reduce the bacterial counts to below the threshold for detecting bacteria. The OXY, NANO, and SSD treatments had similar reductions in bacterial recovery on D3 and D6 of approximately 2.5-4 log. The histological healing metrics of reepithelialization percentage, epithelial thickness, white cell infiltration, angiogenesis, and granulation tissue formation were similar among wounds from POWD, SSD, and NEG groups at 6 days post-infection. Silver oxynitrate powder reduced P. aeruginosa growth in burn wounds more effectively than other silver-based dressings but did not impact wound healing.

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