scholarly journals Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders

2016 ◽  
Vol 68 (4) ◽  
pp. 873-881 ◽  
Author(s):  
D.B.C. Lima ◽  
T.F.P. Silva ◽  
A. Aquino Cortez ◽  
J.N. Pinto ◽  
F.F. Magalhães ◽  
...  
Keyword(s):  
System Analysis ◽  
Sperm Quality ◽  
Domestic Cats ◽  
Epididymal Sperm ◽  
Breeding Programs ◽  
Membrane Function ◽  
Progressive Motility ◽  
Artificial Breeding ◽  
Sperm Membrane ◽  
Flotation Method

ABSTRACT We aimed to compare fresh sperm and sperm cooled to 4ºC that had been recovered from the epididymides of cats using powdered coconut water (ACP-117c) and Tris extenders. Sixty epididymides were divided into 6 groups: 10 fresh epididymides were recovered using Tris (T0h); 10 were kept at 4°C/2h and recovered using Tris (T2h); 10 were kept at 4°C/4h and recovered using Tris (T4h); 10 fresh were recovered using ACP-117c (A0h); 10 were kept at 4°C/2h and recovered using ACP-117c (A2h), and 10 were kept at 4°C/4h and recovered using ACP-117c (A4h). The testis-epididymis complexes (TEC) control were not cooled. The others were cooled at 4°C for 2 or 4h. The epididymis was separated and the sperm was recovered by the modified flotation method. Sperm kinetic parameters were evaluated by a computer-system analysis, and vigor, viability, concentration, membrane function and morphology of the sperm were assessed under a light microscope. The progressive motility with ACP-117c declined after 2h of cooling, but did not differ between fresh and 4h. The vigor and membrane function were higher in A4h than A0h. The vigor at T2h and T4h were decreased compared to T0h. T0h was higher than A0h for vigor and sperm membrane function. However, after 4h of cooling, ACP-117c maintained a higher percentage of living cells. Feline epididymal sperm quality can be maintained to the degree necessary for artificial breeding programs following cooling and ACP-117c may be successfully used to recover cat sperm that have been cooled for up to 4h.

scholarly journals Comparison of three different media for freezing of epididymal sperm from the African buffalo (Syncerus caffer) and influence of equilibration time on the post-thaw sperm quality

2004 ◽  
Vol 71 (3) ◽  
Author(s):  
F.C. Herold ◽  
K. De Haas ◽  
D. Cooper ◽  
B. Colenbrander ◽  
J.O. Nothling ◽  
...  
Keyword(s):  
South Africa ◽  
Sperm Quality ◽  
The Other ◽  
Semen Parameters ◽  
Equilibration Time ◽  
African Buffalo ◽  
Epididymal Sperm ◽  
Progressive Motility ◽  
Reproductive Techniques ◽  
Buffalo Semen

Assisted reproductive techniques might prove themselves useful tools in producing buffaloes free of specific diseases (BFSD), which are in demand in South Africa. Freezing protocols for African buffalo semen must not only result in good post-thaw qualities, but must also be practical. Epididymal sperm from six mature African buffalo bulls was collected, diluted with three different semen extenders and frozen. Pre-freezing equilibration times between 2 and 9 h were tested. Total and progressive motility, longevity and acrosomal integrity were measured and compared. The use of TriladylTM proved to result in better post-thaw parameters than the other two diluents. Equilibration times of between 4 and 9 h did not influence post-thaw sperm qualities significantly. For some of the treatments, exposure to semen extenders before freezing for less than 4 h resulted in inferior post-thaw semen parameters.

Influence of anaesthetic drugs on the epididymal sperm quality in domestic cats

2011 ◽  
Vol 123 (3-4) ◽  
pp. 265-269 ◽  
Author(s):  
E. Jiménez ◽  
C.C. Pérez-Marín ◽  
Y. Millán ◽  
E. Agüera
Keyword(s):  
Sperm Quality ◽  
Domestic Cats ◽  
Epididymal Sperm

scholarly journals Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus)

2017 ◽  
Vol 62 (No. 3) ◽  
pp. 147-152
Author(s):  
CC Perez-Marin ◽  
E. Jimenez ◽  
EI Aguera
Keyword(s):  
Cold Storage ◽  
Sperm Quality ◽  
Dna Integrity ◽  
Sperm Viability ◽  
Domestic Cats ◽  
Epididymal Sperm ◽  
Normal Morphology ◽  
Group A ◽  
Group B ◽  
Sensitive Parameters

The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides were maintained in saline at 4 °C for 12 h and sperm was then processed and frozen. Motility, morphology, acrosome status, sperm viability and DNA integrity were assessed in epididymal sperm samples before freezing (baseline), at thawing (0 h) and 6 h post-thawing (6 h). Although values were lower in Group B, no significant intergroup difference was observed for any of the parameters tested either at baseline or at 0 h. However, significantly higher values (P < 0.05) were observed in Group A at 6 h for total sperm motility (29.0 ± 2.4% vs 13.0 ± 4.3%), sperm viability (35.2 ± 5.4% vs 15.4 ± 1.4%) and normal morphology (47.6 ± 0.8% vs 40.0 ± 2.1%). It was observed that motility and acrosome status of epididymal sperm are the most sensitive parameters when both types of sperm samples (from fresh epididymis or from 12 h cold-stored epididymis) are frozen-thawed. When sperm quality was assessed 6 h after thawing, spermatozoa precooled in the epididymides showed significantly lower values for motility, viability and morphology than spermatozoa from fresh epididymal samples.

Evaluation of epididymal sperm quality following experimentally induced copper poisoning in male rats

Andrologia ◽  
2011 ◽  
Vol 44 ◽  
pp. 110-116 ◽  
Author(s):  
E. Sakhaee ◽  
L. Emadi ◽  
J. Abshenas ◽  
R. Kheirandish ◽  
O. Azari ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Copper Poisoning ◽  
Experimentally Induced

The Influence of Cigarette Smoking on Sperm Quality and Sperm Membrane Integrity

2016 ◽  
Vol 12 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Nyaz Shelko ◽  
Mohammed F. Hamad ◽  
Mathias Montenarh ◽  
Mohamam E. Hammadeh
Keyword(s):  
Cigarette Smoking ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Sperm Membrane

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

scholarly journals A Simple and Practical Method for Rat Epididymal Sperm Count (Rattus norvegicus)

2015 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
Muhammad Ja’far Luthfi
Keyword(s):  
Rattus Norvegicus ◽  
Sperm Quality ◽  
Sperm Count ◽  
Practical Method ◽  
Quality Analysis ◽  
Minimal Amount ◽  
Sperm Number ◽  
Epididymal Sperm ◽  
Sperm Sample

<p>Sperm sample from epididymal source can be determined its number using minimal amount of equipment. These method will aid researcher and practitioner in sperm quality analysis to determined sperm number rapidly and practically.</p>

Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation

2010 ◽  
Vol 90 (3) ◽  
pp. 389-392 ◽  
Author(s):  
N. Am-in ◽  
R N Kirkwood ◽  
M. Techakumphu ◽  
W. Tantasuparuk
Keyword(s):  
Lipid Peroxidation ◽  
Membrane Permeability ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Group A ◽  
Sperm Membrane ◽  
Fresh Semen

Boars having normal (71.1 ± 1.2%; n = 10) or low (35.12 &plusmn 3.9%; n = 10) sperm motility 24 h after collection were used, and semen was evaluated following storage in Beltsville Thawing Solution (BTS) for 24 h at 18°C. Sperm lipids were extracted and lipid peroxidation quantified. No differences were evident in fresh semen, but after 24 h, sperm motility, viability and membrane permeability in the low motility group were lower (P < 0.001) compared with the normal motility group. Sperm membrane lipid peroxidation was greater (P < 0.001) in the low motility group. A factor influencing sperm storability is membrane lipid peroxidation, which can be accurately assayed using a commercial kit.Key words: Boars, sperm motility, sperm quality, lipid peroxidation

scholarly journals The Effect of Kallikrein on Human Sperm Membrane Function.

1991 ◽  
Vol 164 (1) ◽  
pp. 23-28 ◽  
Author(s):  
NIKOLAOS SOFIKITIS ◽  
IKUO MIYAGAWA ◽  
TOSHIKO TODA ◽  
TASUKU HARADA ◽  
YASUYUKI MIO ◽  
...  
Keyword(s):  
Human Sperm ◽  
Membrane Function ◽  
Sperm Membrane

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 301-307 ◽  
Author(s):  
José A. B. Bezerra ◽  
Andréia M. Silva ◽  
Patrícia C Sousa ◽  
Lívia B. Campos ◽  
Érica C. G. Praxedes ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Semen Analysis ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Coconut Water ◽  
Computer Assisted ◽  
Epididymal Sperm ◽  
Sperm Plasma Membrane ◽  
Pecari Tajacu ◽  
Functional Membrane

SummaryThe aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

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