Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil

ABSTRACT: Equine granulocytic anaplasmosis is caused by Anaplasma phagocytophilum, a gram negative, obligatory intracellular bacterium, member of Anaplasmataceae family, included in the Rickettsiales order. Little is known about the disease, transmission dynamics, genetic diversity and prevalence in Minas Gerais state, Brazil. This work aimed to do a serosurvey using indirect immunofluorescent assay (IFA) test and evaluation of buffy coat smears, and nested polymerase chain reaction (PCR) as diagnostic methods, to determine the disease situation in horses from two manga-larga marchador breeding farms located in the municipalities of Ataléia e São Vicente de Minas, in Minas Gerais state. It was found that 76% (131/172) of the animals were considered reactive for IFA test, and the total of 12.8% was positive at buffy coat smears analysis. At PCR analysis, it was found 1.94% of the samples positive to the infection. Those samples were sequenced and showed 96% of similarity to A. phagocytophilum from a Ixodes ricinus tick. There is a high frequency of animals with the evidence of contact to A. phagocytophilum on the two evaluated properties in this study, which was proved by positiveness in PCR analysis. New researches must be carried out to better understand the epidemiologic and clinical dynamic of the disease in the state of Minas Gerais.

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Use of Indicator Kriging to Investigate Schistosomiasis in Minas Gerais State, Brazil

Journal of Tropical Medicine ◽

10.1155/2012/837428 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Ricardo J. P. S. Guimarães ◽

Corina C. Freitas ◽

Luciano V. Dutra ◽

Carlos A. Felgueiras ◽

Sandra C. Drummond ◽

...

Keyword(s):

Spatial Distribution ◽

Information Processing ◽

Geographic Information Systems ◽

Disease Transmission ◽

Processing System ◽

Minas Gerais ◽

Indicator Kriging ◽

Intermediate Hosts ◽

Minas Gerais State ◽

And Control

Geographic Information Systems (GISs) are composed of useful tools to map and to model the spatial distribution of events that have geographic importance as schistosomiasis. This paper is a review of the use the indicator kriging, implemented on the Georeferenced Information Processing System (SPRING) to make inferences about the prevalence of schistosomiasis and the presence of the species ofBiomphalaria, intermediate hosts ofSchistosoma mansoni, in areas without this information, in the Minas Gerais State, Brazil. The results were two maps. The first one was a map ofBiomphalariaspecies, and the second was a new map of estimated prevalence of schistosomiasis. The obtained results showed that the indicator kriging can be used to better allocate resources for study and control of schistosomiasis in areas with transmission or the possibility of disease transmission.

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PCR-based diagnosis for Chagas' disease in Bolivian children living in an active transmission area: comparison with conventional serological and parasitological diagnosis

Parasitology ◽

10.1017/s0031182096008554 ◽

1997 ◽

Vol 114 (4) ◽

pp. 367-373 ◽

Cited By ~ 38

Author(s):

P. WINCKER ◽

J. TELLERIA ◽

M. F. BOSSENO ◽

M. A. CARDOSO ◽

P. MARQUES ◽

...

Keyword(s):

Chagas Disease ◽

Dna Sequences ◽

Disease Transmission ◽

Buffy Coat ◽

Diagnostic Methods ◽

Large Field ◽

Recent Infection ◽

Parasitological Diagnosis ◽

Significant Difference ◽

Transmission Area

A large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnostic methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buffy coat examination, 106 were detected by PCR (sensitivity: 93·8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, IgG-, IgM-, and buffy coat-negative) only 1 case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections.

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Income Distribution and Poverty in Minas Gerais State, Brazil

SSRN Electronic Journal ◽

10.2139/ssrn.262033 ◽

2001 ◽

Author(s):

Luiz Fernando Alves ◽

Rosa Rosa Fontes

Keyword(s):

Income Distribution ◽

Minas Gerais ◽

Minas Gerais State

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Extreme rainfall and its impacts in the Brazilian Minas Gerais state in January 2020: Can we blame climate change?

Climate Resilience and Sustainability ◽

10.1002/cli2.15 ◽

2021 ◽

Author(s):

Ricardo Dalagnol ◽

Carolina B. Gramcianinov ◽

Natália Machado Crespo ◽

Rafael Luiz ◽

Julio Barboza Chiquetto ◽

...

Keyword(s):

Climate Change ◽

Extreme Rainfall ◽

Minas Gerais ◽

Minas Gerais State

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Paleoenvironmental reconstruction of the headwaters of the preto river, Minas Gerais state, Brazil, through siliceous bioindicators

Journal of South American Earth Sciences ◽

10.1016/j.jsames.2021.103349 ◽

2021 ◽

Vol 108 ◽

pp. 103349

Author(s):

David Oldack Barcelos Ferreira Machado ◽

Karina Ferreira Chueng ◽

Heloisa Helena Gomes Coe ◽

Alexandre Christófaro Silva ◽

Camila Rodrigues Costa

Keyword(s):

Minas Gerais ◽

Paleoenvironmental Reconstruction ◽

Minas Gerais State

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Presence of subclinical infection in gene-targeted human prion protein transgenic mice exposed to atypical bovine spongiform encephalopathy

Journal of General Virology ◽

10.1099/vir.0.052738-0 ◽

2013 ◽

Vol 94 (12) ◽

pp. 2819-2827 ◽

Cited By ~ 11

Author(s):

Rona Wilson ◽

Karen Dobie ◽

Nora Hunter ◽

Cristina Casalone ◽

Thierry Baron ◽

...

Keyword(s):

Human Health ◽

Bovine Spongiform Encephalopathy ◽

Prion Protein ◽

Disease Transmission ◽

Large Scale ◽

Single Agent ◽

Diagnostic Methods ◽

Transmissible Spongiform Encephalopathies ◽

Spongiform Encephalopathy ◽

Human Prion Protein

The transmission of bovine spongiform encephalopathy (BSE) to humans, leading to variant Creutzfeldt–Jakob disease has demonstrated that cattle transmissible spongiform encephalopathies (TSEs) can pose a risk to human health. Until recently, TSE disease in cattle was thought to be caused by a single agent strain, BSE, also known as classical BSE, or BSE-C. However, due to the initiation of a large-scale surveillance programme throughout Europe, two atypical BSE strains, bovine amyloidotic spongiform encephalopathy (BASE, also named BSE-L) and BSE-H have since been discovered. To model the risk to human health, we previously inoculated these two forms of atypical BSE (BASE and BSE-H) into gene-targeted transgenic (Tg) mice expressing the human prion protein (PrP) (HuTg) but were unable to detect any signs of TSE pathology in these mice. However, despite the absence of TSE pathology, upon subpassage of some BASE-challenged HuTg mice, a TSE was observed in recipient gene-targeted bovine PrP Tg (Bov6) mice but not in HuTg mice. Disease transmission from apparently healthy individuals indicates the presence of subclinical BASE infection in mice expressing human PrP that cannot be identified by current diagnostic methods. However, due to the lack of transmission to HuTg mice on subpassage, the efficiency of mouse-to-mouse transmission of BASE appears to be low when mice express human rather than bovine PrP.

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