scholarly journals Characterization of Pseudomonas bacteria of Piper tuberculatum regarding the production of potentially bio-stimulating compounds for plant growth

2021 ◽  
Vol 51 (1) ◽  
pp. 10-19
Author(s):  
Danyllo Amaral de OLIVEIRA ◽  
Solange da Cunha FERREIRA ◽  
Daiany Larissa Ribeiro CARRERA ◽  
Cleyson Pantoja SERRÃO ◽  
Daihany Moraes CALLEGARI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Black Pepper ◽  
Plant Diseases ◽  
Piper Nigrum ◽  
Soluble Phosphate ◽  
High Concentration ◽  
Molybdenum Blue ◽  
Iaa Production

ABSTRACT Despite advances in the identification and characterization of endophytic bacteria in various plant species worldwide, little is known about such microorganisms in plants from the Amazon region. Previous studies reported that Piper tuberculatum endophytic Pseudomonas (isolates Pt12 and Pt13, identified as Pseudomonas putida and Pseudomonas sp., respectively) were able to inhibit the in vitro growth of Fusarium solani f. sp. piperis, which causes root rot in black pepper (Piper nigrum), and that Pt13 promoted the growth of P. nigrum. Therefore, the aim here was to characterize these bacteria regarding their ability to produce plant growth-promoting substances [siderophores, indol acetic acid (IAA) and soluble phosphate]. Chrome azurol S assays were performed for the detection of siderophores. For qualitative and quantitative assays of IAA production and phosphate solubilization, Salkowski´s reagent and NBRIP medium with molybdenum blue reagent, respectively, were used. Results revealed that Pt12 and Pt13 were able to synthesize IAA, mainly under a high concentration of L-tryptophan, indicating that they are IAA-producing bacteria, probably through a tryptophan-dependent biosynthesis pathway. The presence of P. nigrum extract positively influenced the IAA production by Pt12 and Pt13, with highest values of 125 and 90 µg mL-1, respectively. In addition, Pt12 was positive for the production of siderophores and produced 56.56 µg mL-1 of soluble phosphate. In contrast, Pt13 showed no ability to produce siderophores or to solubilize phosphate. Besides their potential in controlling plant diseases, Pt12 and Pt13 have potential as biofertilizers, favoring sustainable agriculture.

scholarly journals Isolation and Characterization of Rhizobacteria for their Many Activities Improving the Plant Growth

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Silini ALLAOUA ◽  
Cherif HAFSA ◽  
Ghoul MOSTEFA
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacterial Strains ◽  
High Concentration ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Positive Effect

The use of microbial technology in agriculture extends currently relatively quickly with the identification of new more efficient bacterial strains in the growth of plants. The rhizobacteria that promote the growth of plants (PGPR, plant growth promoting rhizobacteria) can have a positive effect on the productivity of crops( Chaiharn et al., 2008) especially when subjected to salt stress ( Tripathi et al., 1998). Indeed, the salinity considerably affects the crop yield. Inoculating PGPR can mitigate the effects of this type of stress by direct or indirect mechanisms (Ahmad et al., 2008). To select effective strains and having multiple activities, a total of 22 bacterial strains are isolated from different soils. These strains are characterized by their ability to fix nitrogen (nitrogen-free medium culture). They are also tested in vitro for their activities of improvement of plant growth such as the production of the indole acetic acid (IAA) of hydrogen cyanide (HCN), of the siderophore, ammonia (NH3), of solubilization of phosphates and antifungal activity. More than 70% of the strains produce NH3, 54% solubilized more P2O5 10µg/ml and the same rate and has antifungal activity against ≥ to three fungal strains. However, 36% produce more 80µg/ml of IAA, 27% produce siderophores and only two strains produced of HCN. The production of IAA of eight (08) strains is evaluated in the presence of 100, 200 and 300 mM NaCl. The remaining active strains with high concentration of NaCl would be the most effective and can be considered as biofertilizers for improving plant growth in areas affected by salinity.

scholarly journals Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State, Brazil

2021 ◽  
Vol 22 (2) ◽  
pp. 890
Author(s):  
Luccas M. Barata ◽  
Eloísa H. Andrade ◽  
Alessandra R. Ramos ◽  
Oriel F. de Lemos ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Black Pepper ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Volatile Components ◽  
Chemical Compositions ◽  
Multivariate Statistical ◽  
Protein Targets ◽  
Fruit Samples ◽  
Sesquiterpene Hydrocarbons

This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g−1 FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL−1), and carotenoids (0.21–2.31 µg·mL−1) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State.

Elaboration and characterization of O/W cinnamon (Cinnamomum zeylanicum) and black pepper (Piper nigrum) emulsions

2018 ◽  
Vol 77 ◽  
pp. 902-910 ◽  
Author(s):  
Maribel Jiménez ◽  
Jazmín A. Domínguez ◽  
Luz A. Pascual-Pineda ◽  
Ebner Azuara ◽  
C.I. Beristain
Keyword(s):  
Black Pepper ◽  
Piper Nigrum ◽  
Cinnamomum Zeylanicum

scholarly journals Influence of temperature, light and plant growth regulators on germination of black pepper (Piper nigrum L.) seeds

2010 ◽  
Vol 9 (9) ◽  
pp. 1354-1358 ◽  
Author(s):  
Li Zhigang ◽  
Liu Aiqin ◽  
Wu Huasong ◽  
Tan Lehe ◽  
Long Yuzhou ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Influence Of Temperature

scholarly journals In vitro toxin production by Fusarium solani f. sp. piperis

2003 ◽  
Vol 28 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Maria de Lourdes R. Duarte ◽  
Simon A. Archer
Keyword(s):  
Fusarium Solani ◽  
Biological Response ◽  
Black Pepper ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
Detached Leaves

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of static incubation on potato sucrose broth at 25 ºC under illumination. Changes in the pH of the culture filtrate did not alter the effect of toxic metabolites. However, when the pH was changed before the medium had been autoclaved, a more intense biological response was observed, with an optimum at pH 6.0. Isolates that produced red pigments in liquid cultures were more efficient in producing biologically active culture filtrates than those which produced pink coloured or clear filtrates suggesting that these pigments could be related to toxigenic activity. Detached leaves of seven black pepper cultivars and Piper betle showed symptoms of vein discoloration after immersion in autoclaved and non-autoclaved Fsp culture filtrates indicating the thermostable nature of these toxic metabolites.

scholarly journals SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO

2016 ◽  
Vol 4 (1) ◽  
pp. 27 ◽  
Author(s):  
Edi Husen
Keyword(s):  
Plant Growth ◽  
Soil Bacteria ◽  
Phosphate Solubilization ◽  
Azotobacter Vinelandii ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Chelating Agent ◽  
Plant Growth Promoting Rhizobacteria ◽  
Iaa Production

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloric acid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate was positively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65) were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.

Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration

2021 ◽  
Vol 32 (4) ◽  
pp. 555-560
Author(s):  
Samirah ◽  
Aniek Setiya Budiatin ◽  
Ferdiansyah Mahyudin ◽  
Junaidi Khotib
Keyword(s):  
Compressive Strength ◽  
Bone Regeneration ◽  
Mtt Assay ◽  
Local Treatment ◽  
Swelling Ratio ◽  
High Concentration ◽  
Proliferation And Differentiation ◽  
Bovine Hydroxyapatite

Abstract Objectives Alendronate are widely used in the treatment of bone disorders characterized by inhibit osteoclast-mediated bone resorption such as Paget’s disease, fibrous dysplasia, myeloma, bone metastases and osteoporosis. In recent studies alendronate improves proliferation and differentiation of osteoblasts, thereby facilitating for bone regeneration. The disadvantages of this class are their poor bioavailability and side effects on oral and intravenous application such as stomach irritation and osteonecrosis in jaw. Thus, local treatment of alendronate is needed in order to achieve high concentration of drug. Bovine hydroxyapatite-gelatin scaffold with alendronate was studied. Glutaraldehyde was used as cross-linking agent, increase the characteristics of this scaffold. The objectives of this study were to manufacture and characterize alendronate scaffold using bovine hydroxyapatite-gelatin and crosslinked by glutaraldehyde. Methods Preparation of cross-linked bovine hydroxyapatite-gelatin and alendronate scaffold with different concentration of glutaraldehyde (0.00, 0.50, 0.75, and 1.00%). The scaffolds were characterized for compressive strength, porosity, density, swelling ratio, in vitro degradation, and cytotoxicity (the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, shorted as MTT assay). Results Bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked with glutaraldehyde showed lower density than without glutaraldehyde. As glutaraldehyde concentration increased, porosity also increased. Eventually, it reduced compressive strength. Swelling ratio and in vitro degradation was negatively dependent on glutaraldehyde concentration. In addition, the scaffold has a good safety by MTT assay. Conclusions Bovine hydroxyapatite-gelatin-alendronate scaffold was fabricated with various concentrations of glutaraldehyde. The presence of glutaraldehyde on bovine hydroxyapatite-gelatin-alendronate is safe and suitable candidate scaffold for bone regeneration.

Isolation and Characterization of a Soluble Phosphate Hydrolysing Activity from an in vitro Coffee Cell Line Grown in the Presence of Aluminum

2007 ◽  
Vol 2 (5) ◽  
pp. 302-313
Author(s):  
Minero-Garcia Yereni . ◽  
Villanueva Marco A. . ◽  
Vazquez-Flota Felipe . ◽  
Hernandez-Sotomayor . ◽  
Islas-Flores Ignacio .
Keyword(s):  
Cell Line ◽  
Soluble Phosphate ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document