scholarly journals Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State, Brazil

2021 ◽  
Vol 22 (2) ◽  
pp. 890
Author(s):  
Luccas M. Barata ◽  
Eloísa H. Andrade ◽  
Alessandra R. Ramos ◽  
Oriel F. de Lemos ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Black Pepper ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Volatile Components ◽  
Chemical Compositions ◽  
Multivariate Statistical ◽  
Protein Targets ◽  
Fruit Samples ◽  
Sesquiterpene Hydrocarbons

This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g−1 FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL−1), and carotenoids (0.21–2.31 µg·mL−1) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State.

scholarly journals Volatiles of Black Pepper Fruits (Piper nigrum L.)

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4244 ◽  
Author(s):  
Dosoky ◽  
Satyal ◽  
Barata ◽  
da Silva ◽  
Setzer
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Coastal Region ◽  
Hierarchical Cluster ◽  
Black Pepper ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Tropical Regions ◽  
Geographical Regions

Black pepper (Piper nigrum) is historically one of the most important spices and herbal medicines, and is now cultivated in tropical regions worldwide. The essential oil of black pepper fruits has shown a myriad of biological activities and is a commercially important commodity. In this work, five black pepper essential oils from eastern coastal region of Madagascar and six black pepper essential oils from the Amazon region of Brazil were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components of the essential oils were α-pinene, sabinene, β-pinene, δ-3-carene, limonene, and β-caryophyllene. A comparison of the Madagascar and Brazilian essential oils with black pepper essential oils from various geographical regions reported in the literature was carried out. A hierarchical cluster analysis using the data obtained in this study and those reported in the literature revealed four clearly defined clusters based on the relative concentrations of the major components.

scholarly journals The Study on Extraction Process and Analysis of Components in Essential Oils of Black Pepper (Piper nigrum L.) Seeds Harvested in Gia Lai Province, Vietnam

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 56 ◽  
Author(s):  
Thien Hien Tran ◽  
Le Ke Ha ◽  
Duy Chinh Nguyen ◽  
Tan Phat Dao ◽  
Le Thi Hong Nhan ◽  
...  
Keyword(s):  
Essential Oil ◽  
Respiratory Infections ◽  
Mesh Size ◽  
Extraction Process ◽  
Black Pepper ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Efficient Technology ◽  
Preservation Method

Black pepper (Piper nigrum L.) is a tropical crop with extensive medicinal potential in ethnomedicine and nutraceutical applications. The essential oil of black pepper finds wide applications in inhabitation of respiratory infections and soothing of muscular pains due to its warming and energizing property. The pungent bioactive piperine is responsible for this function, and therefore, efficient technology is required for an optimal extraction process of this compound. In the present article, we have developed a procedure for extracting black pepper essential oil from Vietnam, optimizing conditions that affect the extraction process. The effect of process parameters, namely material size, preservation method, the concentration of sodium chloride, the concentration of soak time, the ratio of material to water, temperature extraction, time extraction on the extraction yield, and relative efficiency were investigated. Results demonstrated that 20 g of black pepper milled with a mesh size of 160 obtained 0.48 g of essential oil (2.4%) at a raw material to water ratio of 1/21 (g/mL) at 150 °C in a time of 5.2 h. GC-MS (Gas chromatography–mass spectrometry) spectra showed that 3-carene (29.21%), D-limonene (20.94%), caryophyllene (15.05%), and β-pinene (9.77%) were present as major components. These results suggested that the essential oil extracted from Vietnamese black pepper is applicable in the manufacturing processes of insecticides and air deodorizers.

scholarly journals Characterization of Pseudomonas bacteria of Piper tuberculatum regarding the production of potentially bio-stimulating compounds for plant growth

2021 ◽  
Vol 51 (1) ◽  
pp. 10-19
Author(s):  
Danyllo Amaral de OLIVEIRA ◽  
Solange da Cunha FERREIRA ◽  
Daiany Larissa Ribeiro CARRERA ◽  
Cleyson Pantoja SERRÃO ◽  
Daihany Moraes CALLEGARI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Black Pepper ◽  
Plant Diseases ◽  
Piper Nigrum ◽  
Soluble Phosphate ◽  
High Concentration ◽  
Molybdenum Blue ◽  
Iaa Production

ABSTRACT Despite advances in the identification and characterization of endophytic bacteria in various plant species worldwide, little is known about such microorganisms in plants from the Amazon region. Previous studies reported that Piper tuberculatum endophytic Pseudomonas (isolates Pt12 and Pt13, identified as Pseudomonas putida and Pseudomonas sp., respectively) were able to inhibit the in vitro growth of Fusarium solani f. sp. piperis, which causes root rot in black pepper (Piper nigrum), and that Pt13 promoted the growth of P. nigrum. Therefore, the aim here was to characterize these bacteria regarding their ability to produce plant growth-promoting substances [siderophores, indol acetic acid (IAA) and soluble phosphate]. Chrome azurol S assays were performed for the detection of siderophores. For qualitative and quantitative assays of IAA production and phosphate solubilization, Salkowski´s reagent and NBRIP medium with molybdenum blue reagent, respectively, were used. Results revealed that Pt12 and Pt13 were able to synthesize IAA, mainly under a high concentration of L-tryptophan, indicating that they are IAA-producing bacteria, probably through a tryptophan-dependent biosynthesis pathway. The presence of P. nigrum extract positively influenced the IAA production by Pt12 and Pt13, with highest values of 125 and 90 µg mL-1, respectively. In addition, Pt12 was positive for the production of siderophores and produced 56.56 µg mL-1 of soluble phosphate. In contrast, Pt13 showed no ability to produce siderophores or to solubilize phosphate. Besides their potential in controlling plant diseases, Pt12 and Pt13 have potential as biofertilizers, favoring sustainable agriculture.

scholarly journals Optimization of Essential Oil Extraction Process of White Pepper (Piper nigrum L.) Harvested in Phu Quoc Island, Kien Giang Province, Vietnam

2020 ◽  
Vol 32 (11) ◽  
pp. 2707-2712
Author(s):  
Thien Hien Tran ◽  
Thi Cam Quyen Ngo ◽  
Hoang Duy Ngo ◽  
Nguyen Huu Thuan Anh ◽  
Ton Nu Thuy An ◽  
...  
Keyword(s):  
Essential Oil ◽  
Extraction Process ◽  
Black Pepper ◽  
Oil Extraction ◽  
Extraction Time ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Extraction Temperature ◽  
Green Pepper ◽  
White Pepper

Vietnam is a leading producer and exporter of products harvested from plants of the family Piperaceae such as green pepper, black pepper, red pepper and white pepper. In this study, an attempt is made to examine the essential oil extraction process with the material of white pepper grown and harvested in Phu Quoc island, Vietnam. To maximize essential oil production, three factors consisting the ratio of materials and solvents, extraction time and extraction temperature were selected and optimized. The surface response methodology optimization resulted the highest yield of 3.6%, achieved at the ratio of material and water ratio of 1:20 g/g, extraction time of 96 min and at 130 ºC. A high F values, low P values (< 0.0001), the determination coefficient (R2 = 0.9993) and a non-significant lack of fit suggested a strong correlation between actual and predicted values of the responses. The essential oil obtained was determined for chemical composition by gas chromatography-mass spectrometry (GC-MS) method. The GC-MS results showed that major constituents existing in the oil sample were limonene, 3-carene, sabinene, β-pinene, α-pinene and α-phellandrene, accounting for 27.059, 23.345, 17.903, 9.996, 5.167 and 4.588%, respectively.

scholarly journals Biological Activities and Chemical Constituents of Essential Oils from Piper cubeba Bojer and Piper nigrum L.

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1876 ◽  
Author(s):  
Yusuf Andriana ◽  
Tran Dang Xuan ◽  
Tran Ngoc Quy ◽  
Hoang-Dung Tran ◽  
Quang-Tri Le
Keyword(s):  
Mass Spectrometry ◽  
Essential Oils ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Herbicidal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Chemical Compositions ◽  
Ionization Mass

In this study, we evaluated antioxidant, antihyperuricemic, and herbicidal activities of essential oils (EOs) from Piper cubeba Bojer and Piper nigrum L.; two pepper species widely distributed in tropics, and examined their chemical compositions. Dried berries of P. cubeba and P. nigrum were hydro-distilled to yield essential oil (EO) of 1.23 and 1.11% dry weight, respectively. In the antioxidant assay, the radical scavenging capacities of P. cubeba EO against DPPH and ABTS free radicals were 28.69 and 24.13% greater than P. nigrum, respectively. In the antihyperuricemic activity, P. cubeba EO also exhibited stronger inhibitory effects on xanthine oxidase (IC50 = 54.87 µg/mL) than P. nigrum EO (IC50 = 77.11 µg/mL). In the herbicidal activity, P. cubeba EO showed greater inhibition on germination and growth of Bidens pilosa and Echinochloa crus-galli than P. nigrum EO. Besides, P. cubeba EO decreased 15.98–73.00% of photosynthesis pigments of B. pilosa and E. crus-galli, while electrolyte leakages, lipid peroxidations, prolines, phenolics, and flavonoids contents were increased 10.82–80.82% at 1.93 mg/mL dose. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that P. nigrum and P. cubeba EOs principally possessed complex mixtures of monoterpenes and sesquiterpenes. Terpinen-4-ol (42.41%), α-copaene (20.04%), and γ-elemene (17.68%) were the major components of P. cubeba EO, whereas β-caryophyllene (51.12%) and β-thujene (20.58%) were the dominant components of P. nigrum EO. Findings of this study suggest both P. cubeba and P. nigrum EOs were potential to treat antioxidative stress and antihyperuricemic related diseases. In addition, the EOs of the two plants may be useful to control B. pilosa and E. crus-galli, the two invasive and problematic weeds in agriculture practice.

A new Dorylaimid nematode Xiphinema radicicola n. sp.

1936 ◽  
Vol 14 (2) ◽  
pp. 69-72 ◽  
Author(s):  
T. Goodey
Keyword(s):  
Black Pepper ◽  
Plant Diseases ◽  
Piper Nigrum ◽  
East Indies ◽  
Large Numbers ◽  
Aphelenchus Avenae ◽  
Burrowing Nematode ◽  
The Subject ◽  
Dutch East Indies ◽  
De Man

In a collection of nematodes received from the Institute of Plant Diseases, Buitenzorg, Java, there were three tubes containing eelworms from pepper gardens on the island of Bangka, Dutch East Indies, where disease of black pepper (Piper nigrum L.) caused by the “burrowing” nematode, Anguillulina similis, is particularly severe. In one of these tubes were worms which had been collected by Dr. J. van der Vecht from diseased pepper roots grown in a garden at Mabat Oerit, Bangka. Among these were large numbers of Anguillulina similis, many Cephalobus striatus, one Aphelenchus avenae, a few Dorylaimus sp. and 30 representatives of the worm which forms the subject of this note. On being cleared and mounted in glycerine it was seen that the buccal spear in all cases was extraordinarily long and closely resembled that figured by Cobb (1913) in Xiphinema americanum from which it was concluded that the worms might possibly belong to the genus Xiphinema. A question which exercised the writer for some time was whether, following Micoletzky (1921), the genus Xiphinema Cobb, 1913 should be considered as synonymous with Tylencholaimus de Man, 1876.

scholarly journals Rapid Characterization and Discovery of Chemical Markers for Discrimination of Xanthii Fructus by Gas Chromatography Coupled to Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4079
Author(s):  
Kim ◽  
Jung ◽  
Jeon ◽  
Hwang ◽  
Ahn
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Chemical Markers ◽  
Multivariate Statistical ◽  
Specific Chemical ◽  
Xanthium Sibiricum ◽  
Rapid Characterization ◽  
Xanthium Canadense

Xanthii Fructus (XF) is known as a medicinal plant. It has been used as a traditional medicine because of its high biological efficacy. However, there have been few comprehensive studies on the specific chemical composition of the plant and consequently, the information is lacking for the mechanism of the natural product metabolites in humans. In this study, an efficient analytical method to characterize and discriminate two species of Xanthii Fructus (Xanthium canadense Mill. and Xanthium sibiricum Patrin ex Widder) was established. Volatile organic compounds (VOCs), polar metabolites, and fatty acids were classified by integrated sample preparation, which allowed a broad range for the detection of metabolites simultaneously. Gas chromatography-mass spectrometry (GC-MS) followed by a multivariate statistical analysis was employed to characterize the chemical compositions and subsequently to discriminate between the two species. The results demonstrate that the two species possess obviously diverse chemical characteristics of three different classifications, and discriminant analysis was successfully applied to a number of chemical markers that could be used for the discrimination of the two species. Additional quantitative results for the selected chemical markers consistently showed significant differences between the two species.

scholarly journals PipeNig®-FL, a Fluid Extract of Black Pepper (Piper Nigrum L.) with a High Standardized Content of Trans-β-Caryophyllene, Reduces Lipid Accumulation in 3T3-L1 Preadipocytes and Improves Glucose Uptake in C2C12 Myotubes

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2788 ◽  
Author(s):  
Federica Geddo ◽  
Rosaria Scandiffio ◽  
Susanna Antoniotti ◽  
Erika Cottone ◽  
Giulia Querio ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Glucose Uptake ◽  
Lipid Accumulation ◽  
Cannabinoid Receptor ◽  
Adipogenic Differentiation ◽  
Black Pepper ◽  
Metabolic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
C2c12 Myotubes

Trans-β-caryophyllene (BCP) is a natural sesquiterpene hydrocarbon with several important pharmacological activities, including antioxidant, anti-inflammatory, anticancer, and cardioprotective functions. These properties are mainly due to its selective interaction with the peripherally expressed cannabinoid receptor 2. In addition, BCP activates peroxisome proliferated activator receptors α and γ and inhibits the Toll-like receptor signaling pathway. Given the growing scientific interest in BCP, the aim of our study was to investigate the metabolic effects of a black pepper extract (PipeNig®-FL), containing a high standardized content of BCP. In particular our interest was focused on its potential activity on lipid accumulation and glucose uptake. The extract PipeNig®-FL was chemically characterized by gas chromatography–mass spectrometry (GC–MS) and gas chromatography with flame-ionization detection (GC–FID), confirming a high content (814 mg/g) of BCP. Experiments were performed on 3T3-L1 preadipocytes and on C2C12 myotubes. Lipid content following 3T3-L1 adipogenic differentiation was quantified with AdipoRed fluorescence staining. Glucose uptake and GLUT4 membrane translocation were studied in C2C12 myotubes with the fluorescent glucose analog 2-NBDG and by immunofluorescence analysis. Here we show that PipeNig®-FL reduces 3T3-L1 adipocyte differentiation and lipid accumulation. Moreover, acute exposure of C2C12 myotubes to PipeNig®-FL improves glucose uptake activity and GLUT4 migration. Taken together, these results reveal interesting and novel properties of BCP, suggesting potential applications in the prevention of lipid accumulation and in the improvement of glucose uptake.

scholarly journals Chemical compositions of essential oils from two Artemisia species used in Mongolian traditional medicine

2018 ◽  
Vol 18 (44) ◽  
pp. 48-51
Author(s):  
Javzmaa N ◽  
Altantsetseg Sh ◽  
Shatar S ◽  
Amarjargal A
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Steppe Zone ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Medicinal Products ◽  
Diverse Range ◽  
The Family ◽  
Artemisia Species

Essential oils of aromatic and medicinal plants generally have a diverse range of activities because they possess many active constituents that work through a several modes of action. Artemisia, the largest genus of the family Asteraceae, has a number of effects against human and plant diseases. The main purpose of the present study was to investigate chemical compositions of essential oils of two Artemisia species, Artemisia palustris L and Artemisia sericea Weber ex Stechm from the Mongolian steppe zone using gas chromatography and gas chromatography-mass spectrometry. The essential oil of A.palustris was characterized by the presence of monoterpene hydrocarbons such as  trans-β-ocimene (59.1%), cis-β-ocimene (11.6%) and myrcene (7.1%), while the oil of A.sericea was dominated by the presence of three oxygenated monoterpenoids as 1,8-cineole (25.8%), borneol (22.5%) and camphor (18.8%) which are used for preparation of a fragrance and medicinal products.

scholarly journals Essential Oils from Fresh Fruits, Fruit Stalks and Stem Barks of Four Piper nigrum Varieties from Sarawak

1970 ◽  
Vol 3 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Irna Syairina Sahari ◽  
Zaini Assim ◽  
Fasihuddin Badruddin Ahmad ◽  
Ismail Bin Jusoh
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Chemical Compositions ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Oil Components ◽  
Type Apparatus

The variation of chemical compositions in essential oils obtained from fresh fruits, fruit stalks and stem barks offour Piper nigrum L. varieties was analysed using capillary gas chromatography-flame ionization detector (GCFID)and gas chromatography-mass spectrometry (GC-MS). The essential oils were extracted byhydrodistillation Clevenger-type apparatus. The major oil constituents identified in all samples were β-elemene(1.07-2.93%), (+)-δ-cadinene (0.58-6.20%), α-caryophyllene (1.90-6.21%), δ-elemene (0.38-13.26%), 3-carene(0.07-16.82%) and β-caryophyllene (11.78-38.33%). All oil samples showed high content of sesquiterpenes,whereas fruit oils from Semongok Emas and Semongok Wan yielded mostly monoterpenes. Hierarchicalclustering and principal component analysis (PCA) of the oil components revealed significant correlationamongst individual P. nigrum oil samples.

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