scholarly journals Evaluation of the process conditions for the production of microbial carotenoids by the recently isolated Rhodotorula mucilaginosa URM 7409

2019 ◽  
Vol 22 ◽  
Author(s):  
Whallans Raphael Couto Machado ◽  
Lucas Gomes da Silva ◽  
Ellen Silva Lago Vanzela ◽  
Vanildo Luiz Del Bianchi
Keyword(s):  
Experimental Design ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Process Conditions ◽  
Malt Extract ◽  
Rhodotorula Mucilaginosa ◽  
Process Characteristics ◽  
Initial Ph ◽  
Microbial Carotenoids

Abstract This study aimed to improve the physical and nutritional process conditions for the production of carotenoids by the newly isolated Rhodotorula mucilaginosa, a red basidiomycete yeast. The carotenoid bioproduction was improved using an experimental design technique, changing the process characteristics of agitation (130 rpm to 230 rpm) and temperature (25 °C to 35 °C) using seven experiments, followed by a 25-1 fractional design to determine the relevant factors that constitute the culture medium (glucose, malt extract, yeast extract, peptone and initial pH). A complete second order experimental design was then carried out to optimize the composition of the culture medium, the variables being yeast extract (0.5 to 3.5 g/L), peptone (1 to 5 g/L) and the initial pH (5.5 to 7.5), with 17 experiments. The maximum carotenoid production was 4164.45 μg/L (252.99 μg/g), obtained in 144 h in YM (yeast malt) medium with 30 g/L glucose, 10 g/L malt extract, 2 g/L yeast extract, 3 g/L peptone, an initial pH 6, 130 rpm and 25 °C, demonstrating the potential of this yeast as a source of bio-pigments. In this work, the nitrogen sources were the factors that most influenced the intracellular accumulation of carotenoids. The yeast R. mucilaginosa presented high production at a bench level and may be promising for commercial production.

scholarly journals DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

2020 ◽  
Vol 19 (1) ◽  
pp. 11-20
Author(s):  
Aysun Pekşen ◽  
Beyhan Kibar
Keyword(s):  
Yeast Extract ◽  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Malt Extract ◽  
The Public ◽  
Carbon And Nitrogen ◽  
Ph Values ◽  
Incubation Temperatures ◽  
Macrolepiota Procera

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

scholarly journals A Study on L-Asparaginase ofNocardia levisMK-VL_113

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Alapati Kavitha ◽  
Muvva Vijayalakshmi
Keyword(s):  
Yeast Extract ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Initial Ph

An enzyme-based drug, L-asparaginase, was produced byNocardia levisMK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature30∘C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase byN. levis.

scholarly journals OPTIMAL CONDITIONS FOR EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus plantarum T10

2018 ◽  
Vol 54 (4A) ◽  
pp. 40
Author(s):  
Tran Bao Khanh
Keyword(s):  
Lactobacillus Plantarum ◽  
Cell Density ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Optimal Conditions ◽  
Exopolysaccharide Production ◽  
Initial Cell ◽  
Positive Effects ◽  
Mrs Broth

Exopolysaccharide (EPS) production ability of Lactobacillus plantarumT10 was studied. The supplement of some sugars (lactose, saccharose, and glucose) gave the positive effects on EPS production of L. plantarum T10, in which the addition of lactose 4 % resulted in the most efficiency for EPS yield (274.83 μg/mL). The addition of 0.4 % of yeast extract into culture medium with 4 % lactose provided the highest EPS yields compared to other nitrogen sources (peptone, beef extract), which were 378.32 mg/mL. The optimal conditions for EPS production of L. plantarum T10 in MRS broth with 4 % of lactose and 0.4 % yeast extract supplement were also studied. The results indicated that the highest EPS yield (417.11 mg/L) was obtained in the conditions of 106 CFU/ml initial cell density, temperature of 35 oC, pH 5.5 and 48 h incubation.

Croissance et activité cellulaire du Brevibacterium casei NCDO 2049 dans du perméat de lactosérum

1997 ◽  
Vol 43 (12) ◽  
pp. 1180-1188
Author(s):  
K. M. Oulé ◽  
G. Turcotte ◽  
Y. Beaulieu
Keyword(s):  
Growth Factors ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Inhibitory Effect ◽  
Vitamin B ◽  
Cellular Activity ◽  
Whey Permeate ◽  
The Media ◽  
Influence Of Ph

Growth and cellular activity of Brevibacterium casei NCDO 2049 were studied in a whey permeate as basic culture medium. The possible inhibitory effect of the carbone substrate (undiluted or diluted permeate) on growth was investigated as well as the influence of pH of the media (controlled or not) and of the addition of nitrogen sources (organic or inorganic) or growth factors such as yeast extract or vitamin B12. Growth in undiluted permeate produced a maximal biomass (6.5 × 109 cfu/mL) that was nearly twice as much as that in diluted permeate (3.8 × 109 cfu/mL). The carbone substrate (lactose) had no inhibitory effect on growth. In undiluted permeate and an uncontrolled pH, maximal biomass was reached after 36 h of incubation, while in a pH controlled medium, twice as much time was required to obtain an equivalent biomass. In undiluted permeate and an uncontrolled pH, growth in the presence of peptone reached 22.6 × 109 cfu/mL and, in the presence of (NH4)2SO4, 12.4 × 109 cfu/mL. Adding growth factors to media with peptone resulted in the reduction of 90% of initial lactose in the presence of yeast extract and of 75% in the presence of B12 vitamin. This study indicates the possibility of reducing lactose in whey permeate when cultivating strains of the genus Brevibacterium used as maturing bacteria for certain cheese types.Key words: whey permeate, Brevibacterium casei, lactose.[Journal translation]

Validation of Fermentative Parameters for Efficient Succinate Production in Batch Operation by Actinobacillus succinogenes 130ZT

2012 ◽  
Vol 550-553 ◽  
pp. 1448-1454
Author(s):  
Apichai Sawisit ◽  
Supaluk Seesan ◽  
Sitha Chan ◽  
Sunthorn Kanchanatawee ◽  
Sirima Suvarnakuta Jantama ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Actinobacillus Succinogenes ◽  
Brewer’S Yeast ◽  
Succinate Production ◽  
Brewer's Yeast ◽  
Lactose Fermentation ◽  
Initial Ph ◽  
The Cost

Succinate is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present study, the effects of different carbon and nitrogen sources, initial pH of the growth medium (pH 4.5-9.0), and temperature (25-45°C) on the fermentative succinate production by Actinobacillus succinogenes 130ZT were investigated in 100 mL anaerobic bottles. The results revealed that the highest concentration of succinate at 6.28 g/L was produced from 10 g/L of glucose or lactose in the medium containing 5 g/L yeast extract at 24 h. However, a comparable concentration of succinate was also produced when the medium was supplemented with 5 g/L spent brewer’s yeast extract. Based on these results, the cost effectiveness of succinate production could be improved by the use of glucose or lactose fermentation supplemented with spent brewer’s yeast extract. Optimized initial pH at 8.0, temperature at 37 °C, and inoculum size at 6% (v/v) provided the best succinate production at the concentration of 6.37 g/L with a yield of 68.73%.

Optimization and Purifi cation of L-Asparaginase Produced by Streptomyces tendae TK-VL_333

2010 ◽  
Vol 65 (7-8) ◽  
pp. 528-531 ◽  
Author(s):  
Alapati Kavitha ◽  
Muvva Vijayalakshmi
Keyword(s):  
Culture Medium ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Nitrogen Sources ◽  
Laterite Soil ◽  
Factors Affecting ◽  
Carbon And Nitrogen ◽  
Initial Ph ◽  
Streptomyces Tendae

Cultural factors affecting the production of L-asparaginase by Streptomyces tendae isolated from laterite soil samples of Guntur region were investigated on glycerolasparagine- salts (modified ISP-5) broth. Optimal yields of L-asparaginase were recorded in the culture medium with the initial pH 7.0 incubated at 30 °C for 72 h. The strain utilized sucrose (2%) and yeast (2%) extract as carbon and nitrogen sources for L-asparaginase production. The productivity of L-asparaginase was slightly enhanced when the strain was treated with cell-disrupting agents like EDTA. The crude enzyme was purifi ed to homogeneity by ammonium sulfate precipitation, Sephadex G-100 and CM-Sephadex G-50 gel filtration. By employing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight of the enzyme was recorded as 97.4 kDa. This is the first report on production and purification of L-asparaginase from S. tendae.

scholarly journals Indução e caracterização morfológica e bioquímica de calos de Hyptis leucocephala (Lamiaceae)

2012 ◽  
Vol 12 (1) ◽  
pp. 151 ◽  
Author(s):  
Danilo Marcelo Santos Pereira ◽  
Lenaldo Muniz De Oliveira ◽  
Cristina Ferreira Nepomuceno ◽  
José Raniere Ferreira De Santana ◽  
Marília Lordelo Cardoso Silva ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Formation ◽  
Semiarid Region ◽  
In Vitro Cultivation ◽  
Malt Extract ◽  
Organic Additives ◽  
Brazilian Semiarid

Hyptis leucocephala is an aromatic herb endemic to the Brazilian semiarid region, with antimicrobial, antifungal,cytotoxic, anti-inflammatory and anti-HIV properties. In vitro cultivation makes the production of high genetic andsanitary quality cuttings possible for this species, allowing its sustainable exploitation. In the present study, the effect of 6-benzilaminopurine (BAP) and naftalenoacetic acid (NAA) on callus induction in leaf segments of this species anddifferent organic additives to WPM (Wood Plant Medium) for the callus induction was evaluated. The combination ofBAP and NAA allowed the induction of compact and undifferentiated calluses. The highest rate of callus formation andgrowth was obtained with the combination of 8.88 μM BAP and 21.48 μM NAA. Supplementing the culture medium withcoconut water, malt extract and yeast extract did not induce friable calluses. The levels of total sugars, reducing sugars,protein, and amino acids were reduced in calluses maintained in culture medium supplemented with coconut water, maltextract and yeast extract.

scholarly journals Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Noomen Hmidet ◽  
Hanen Ben Ayed ◽  
Philippe Jacques ◽  
Moncef Nasri
Keyword(s):  
Culture Medium ◽  
Immobilized Cells ◽  
Crop Protection ◽  
Nitrogen Sources ◽  
Potential Candidate ◽  
Primary Metabolites ◽  
Initial Ph ◽  
Metabolites Production ◽  
Diesel Biodegradation ◽  
Diesel Degradation

This work concerns the study of the enhancement of surfactin and fengycin production byB. mojavensisA21 and application of the produced product in diesel biodegradation. The influences of the culture medium and cells immobilization were studied. The highest lipopeptides production was achieved after 72 hours of incubation in a culture medium containing 30 g/L glucose as carbon source and a combination of yeast extract (1 g/L) and glutamic acid (5 g/L) as nitrogen sources with initial pH 7.0 at 30°C and 90% volumetric aeration. The study of primary metabolites production showed mainly the production of acetoin, with a maximum production after 24 h of strain growth. The use of immobilized cells seemed to be a promising method for improving lipopeptides productivity. In fact, the synthesis of both lipopeptides, mainly fengycin, was greatly enhanced by the immobilization of A21 cells. An increase of diesel degradation capacity of approximately 20, 27, and 40% in the presence of 0.5, 1, and 2 g/L of produced lipopeptides, respectively, was observed. Considering these properties,B. mojavensisA21 strain producing a lipopeptide mixture, containing both surfactin and fengycin, may be considered as a potential candidate for future use in bioremediation and crop protection.

Optimization of Degrading Poly-Containing Wastewater Conditions of Mixed Bacteria by Orthogonal Experiments

2012 ◽  
Vol 550-553 ◽  
pp. 2386-2390
Author(s):  
Jiang Hong Liu ◽  
Rui Dan Xu ◽  
Yang Pan ◽  
Yuan Ying Wang
Keyword(s):  
Ph Value ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Optimum Process ◽  
Process Conditions ◽  
Activation Time ◽  
Orthogonal Experiments ◽  
Phosphorus Sources ◽  
Initial Ph ◽  
Mixed Bacteria

The factor influencing on the mixed bacteria to degrade wastewater containing poly-containing conditions were optimized. Crude oil as a source of carbon, the effects of nitrogen sources, phosphorus sources, time, inoculation amount, temperature, mineralization content, initial pH value and activation time on degrading poly-containing wastewater were investigated by experiment of single factor and orthogonal experiments. The optimum process conditions were as follows: polyacrylamide (HPAM) which comes from waste water as a source of nitrogen, without other nitrogen source, K2HPO4 was phosphorus sources, the microorganisms activation time was 2, incubation time was 3d, initial pH value was 7, the inoculum size was 5%(v/v), incubation temperature was 35°C, the mineralization degree was 4000 mg•L-1. The main factors affecting degradation rate was nitrogen sources, time, pH, activation time, inoculum size and mineralization content.

MICROBIOLOGICAL DEGRADATION OF QUERCITRIN

1963 ◽  
Vol 9 (2) ◽  
pp. 211-220 ◽  
Author(s):  
D. W. S. Westlake
Keyword(s):  
Carbon Monoxide ◽  
Carboxylic Acid ◽  
Aspergillus Flavus ◽  
Yeast Extract ◽  
Organic Nitrogen ◽  
Culture Medium ◽  
Alternative Pathway ◽  
Nitrogen Sources ◽  
Maximum Production ◽  
Aspergillus Flavus Group

A number of molds and bacteria were screened for their ability to degrade quercitrin. The molds, but not the bacteria, were particularly active and produced carbon monoxide. The degradation of quercitrin is dependent upon the synthesis of an inducible glycosidase (quercitrinase). This enzyme is synthesized by only a few members of the Aspergillus flavus group. Two of these strains synthesized quercitrinase and excreted it and other enzymes into the culture medium. Maximum production of quercitrinase was obtained with organic nitrogen sources such as yeast extract or phytone. Quercitrinase is induced by readily metabolized flavonols and flavonol-glycosides. The glycosidase is quite specific, liberating the rhamnose from the 3-position of quercitrin and myricitrin and the 7-position of robinin. The aglycone, quercetin, is subsequently metabolized to carbon monoxide and the depside of phloroglucinol-carboxylic acid and proto-catechuic acid. Evidence is also presented for an alternative pathway for the metabolism of the flavonol nucleus.

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