Molecular and taxonomic characterisation of introduced specimens of Poecilia reticulata in the lower Paraguay River basin (Cyprinodontiformes: Poeciliidae)

ABSTRACT Poeciliids comprise around 300 species inhabiting the fresh and brackish waters of the Americas and Africa. Poecilia reticulata is native to Northeastern South America and Trinidad and Tobago. In this paper, introduced specimens of P. reticulata collected in the lower Paraguay River in Argentina, were characterized by means of molecular and taxonomic approaches. We further explore, by means of DNA Barcoding, the singularity of the genetic identity of these specimens. Ocurrence of P. reticulata in the lower Paraguay River represents the first record of this species in Argentina. Thirteen individuals of P. reticulata were collected. DNA barcoding showed that all five specimens sampled belong to a single mitochondrial lineage, which was also present in 11 countries from five continents. The distance-based tree clearly grouped separetely four different clusters of P. reticulata when including public data. Genetic distance between the most divergent P. reticulata almost paralleled distance between this species and Poecilia mexicana and P. vivipara. Established populations from Paraguay could be one of the plausible sources for the introduced populations recorded in the lower Paraguay River. The presence of P. reticulata in an open waterway with known drainage to a natural stream is of major concern.

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First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612011000100019 ◽

2011 ◽

Vol 20 (1) ◽

pp. 85-87 ◽

Cited By ~ 6

Author(s):

Santiago Benites de Pádua ◽

Márcia Mayumi Ishikawa ◽

Fabiana Satake ◽

Gabriela Tomas Jerônimo ◽

Fabiana Pilarski

Keyword(s):

Prevalence Rate ◽

First Record ◽

Diagnostic Methods ◽

Mato Grosso ◽

First Report ◽

Paraguay River ◽

Blood Smears ◽

Mato Grosso Do Sul ◽

Pantanal Wetland ◽

Blood Centrifugation

The blood infection by Trypanosoma sp. in tuvira (Gymnotus aff. inaequilabiatus) from the Pantanal wetland was reported in this study. Ten fish from the Paraguay River in the Pantanal were evaluated for the presence of hemoflagellates. Trypomastigotes of Trypanosoma sp. were observed in blood smears from three fish (30% prevalence) and some forms were seen to be undergoing division. Using the diagnostic methods of fresh examination and blood centrifugation in hematocrit capillary tubes, the prevalence rate was 80%. This is the first report of Trypanosoma sp. in tuvira in Brazil.

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STUDI MORFOLOGI DAN FENETIK TAKSONOMI IKAN INTRODUKSI KHAS TELAGA SARI, PASURUAN

Borneo Journal of Biology Education ◽

10.52222/bjbe.v1i1.958 ◽

2019 ◽

Vol 1 (1) ◽

pp. 18-33

Author(s):

Dwi Anggorowati Rahayu ◽

◽

Endik Deni Nugroho ◽

Dwi Listyorini ◽

◽

...

Keyword(s):

Poecilia Reticulata ◽

Poecilia Mexicana

Anggota Famili Poeciliidae merupakan ikan introduksi dengan karakteristik berukuran kecil, live-bearers, dimorfi seksual dari segi ukuran tubuh, pola warna dan struktur gonopodium sebagai ciri diagnostik pada ikan jantan. Ikan ini berperan sebagai pengontrol perkembangan nyamuk malaria dan model pembelajaran biologi. Sejauh ini kajian taksonomi dan penelusuran hubungan kekerabatan didalam famili Poeciliidae belum ada. Tujuan penelitian ini adalah mengidentifikasi jenis, kekerabatan dan pengelompokkan berdasarkan karakter morfologi (morfometrik, meristrik, gonopodium dan ciri diagnostiknya). Jenis penelitian ini adalah deskriptif eksploratif, dengan pendekatan survey. Pada penelitian ini ditemukan tiga spesies ikan Poeciliidae yaitu Xiphoporus helleri, Poecilia mexicana dan Poecilia reticulata. Dendogram hasil analisis morfologi menggunakan metode UPGMA diperoleh dua kelompok percabangan apomorfi yaitu kelompok Genus Xiphoporus dan Genus Poecilia. Sub Clade automorfi antara Xiphoporus helerri A dan B dengan nilai kesamaan 92%. Pengelompokkan ikan Poeciliidae dibedakan berdasarkan lima karakter utama terpilih yaitu: SL (standart length), Hdl (Panjang kepala), Panjang gonopodium, panjang sirip ekor dan PreDL (Panjang sebelum sirip dorsal).Kejelasan identifikasi ikan introduksi khas Telaga Sari menjadi modal utama dalam aquaculture, manajemen, konservasi, biodiversitas dan perkembangan ilmu pengetahuan.

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First record of roving coral grouper Plectropomus pessuliferus (Fowler, 1904) from Saint Martin’s Island, Bangladesh based on Morphology and DNA Barcoding

Bangladesh Journal of Fisheries ◽

10.52168/bjf.2020.32.34 ◽

2021 ◽

Vol 32 (2) ◽

pp. 293-301

Author(s):

MD JAYEDUL ISLAM ◽

SHARMIN AKTER ◽

PROVAKOR SARKAR ◽

MOHAMMAD RASHED ◽

IREEN PARVIN ◽

...

Keyword(s):

Dna Barcoding ◽

Bay Of Bengal ◽

New Record ◽

First Record ◽

Morphological Characters ◽

Marine Waters ◽

First Report ◽

Saint Martin ◽

Northern Bay Of Bengal

A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) wasdocumented based on morphological characters and DNA barcoding. The species was collectedduring a regular survey for making an inventory of reef associated fishes in Saint Martin`sIsland, Bangladesh. This is the first report of roving coral grouper from the marine waters ofBangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the firstreport from the northern Bay of Bengal.

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First Record of Mansonia dyari in the State of Morelos, Mexico, Based on Morphology and COI DNA Barcoding

Journal of the American Mosquito Control Association ◽

10.2987/19-6909.1 ◽

2020 ◽

Vol 36 (1) ◽

pp. 33-36

Author(s):

Luis F. Hernández-Guevara ◽

Francisco J. Sánchez-Rámos ◽

Rahuel J. Chan-Chable ◽

Luis M. Hernández-Triana ◽

Ma. Teresa Valdés-Perezgasga ◽

...

Keyword(s):

Cytochrome C ◽

Dna Barcoding ◽

Cytochrome C Oxidase ◽

Mosquito Species ◽

First Record ◽

Morphological Characters ◽

The State ◽

Historical Records ◽

Cytochrome C Oxidase I ◽

Medical Importance

ABSTRACT Collections of mosquitoes were conducted for the surveillance of species of medical importance in the state of Morelos, Mexico, in June 2017. Species collected included Mansonia (Mansonia) dyari, which was identified using morphological characters and cytochrome c oxidase I DNA barcoding. Although 3 species of genus Mansonia have been previously reported in Mexico, this is the 1st confirmed record of Ma. dyari in Morelos State, where no Mansonia species had been recorded. Historical records of Ma. dyari and Ma. indubitans in Mexico were reviewed. Therefore, this record increases the number of mosquito species occurring in Morelos to 46. The specimens collected in this study were deposited in the Culicidae collection of the Universidad Autónoma Agraria Antonio Narro, Unidad Laguna.

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First Record of African Angel Shark, Squatina africana (Chondricthyes: Squatinidae) in Indian Waters, Confirmed by DNA Barcoding

Journal of Ichthyology ◽

10.1134/s0032945218030013 ◽

2018 ◽

Vol 58 (3) ◽

pp. 312-317 ◽

Cited By ~ 1

Author(s):

M. N. Ambily ◽

P. U. Zacharia ◽

T. M. Najmudeen ◽

L. Ambily ◽

K. T. S. Sunil ◽

...

Keyword(s):

Dna Barcoding ◽

First Record ◽

Indian Waters

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Effect of introduced species and habitat alteration on the occurrence and distribution of euryhaline fishes in fresh- and brackish-water habitats on Aruba, Bonaire and Curaçao (South Caribbean)

Contributions to Zoology ◽

10.1163/18759866-07701006 ◽

2008 ◽

Vol 77 (1) ◽

pp. 45-51 ◽

Cited By ~ 6

Author(s):

H. Hulsman ◽

R. Vonk ◽

M. Aliabadian ◽

A.O. Debrot ◽

V. Nijman

Keyword(s):

Fish Species ◽

Poecilia Reticulata ◽

Fish Fauna ◽

Hierarchical Cluster ◽

First Record ◽

Dry Season ◽

Indigenous Species ◽

Wet Season ◽

Netherlands Antilles ◽

Induced Changes

We conducted an ichthyological survey during the dry season of 2006 on the semi-arid islands of Aruba, Bonaire and Curaçao to provide information on species composition, richness and distribution in natural and non-natural aquatic habitats. The dry season species assemblages (N = 9 species) comprised less species than the wet seasons, and these data refine our knowledge of the indigenous fish fauna and its refuge localities during phases of drought and ensuing high salinity. A hierarchical cluster analysis reveals that the three islands have different species compositions with Curaçao being the most diverse, probably due to its having the most habitats and freshwaters present throughout the year. Species richness was unrelated to salinity and species diversity was highest in canalised streams. In the dry season fewer amphidromous species are present than in the wet season. We found no significant effect of human-induced changes on the presence or absence of fish species in the Netherlands Antilles. The presence of exotic species (including Xiphophorus helleri on Aruba, a first record for this island, and Oreochromis mossambicus and Poecilia reticulata occurring on all three islands) did not have a clear effect on the presence of indigenous species, nor did human alteration of the habitats have an influence on the occurrence of fish species.

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DNA barcoding unveils the first record of Andrena allosa for Italy and unexpected genetic diversity in Andrena praecox (Hymenoptera: Andrenidae)

Fragmenta Entomologica ◽

10.4081/fe.2020.414 ◽

2020 ◽

Vol 52 (1) ◽

pp. 71-75

Author(s):

Maurizio Cornalba ◽

Paolo Biella ◽

Andrea Galimberti

Keyword(s):

Genetic Diversity ◽

Global Change ◽

Dna Barcoding ◽

Species Identification ◽

First Record ◽

Global Distribution ◽

Genetic Identification ◽

Genetic Structuring ◽

Eastern Sector ◽

Morphological Species

DNA barcoding is well-known to support morphological species identification and it can be helpful for unveiling unexpected populations divergence patterns, especially in the context of the impacts on species posed by global change. In this note, we provided the first Italian record of the alpine mining bee Andrena allosa Warncke, 1975, confirmed with DNA barcoding. In addition, genetic identification of a specimen of Andrena praecox (Scopoli 1753) from western Italy pointed to an unexpected intraspecific genetic structuring at COI DNA barcoding region, with sequences from the Italian and the western sector of its global distribution differing 2.22% (p-dist) from populations of the eastern sector. Given the relevance of these records and of the genetic identity of bee populations from Italy, we argue that implementing molecular surveys in bee monitoring would surely contribute to the conservation of these important pollinators.

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