scholarly journals Watery and dark axons in Wallerian degeneration of the opossum's optic nerve: different patterns of cytoskeletal breakdown?

2001 ◽  
Vol 73 (2) ◽  
pp. 231-243 ◽  
Author(s):  
MARCELO S. NARCISO ◽  
JAN NORA HOKOÇ ◽  
ANA M. B. MARTINEZ
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Optic Nerve ◽  
Wallerian Degeneration ◽  
Morphological Analysis ◽  
Axonal Degeneration ◽  
Nerve Fibers ◽  
Unknown Mechanism ◽  
Optic Nerves ◽  
Transmission Electron

In this paper we report a qualitative morphological analysis of Wallerian degeneration in a marsupial. Right optic nerves of opossums Didelphis marsupialis were crushed with a fine forceps and after 24, 48, 72, 96 and 168 hours the animals were anaesthetized and perfused with fixative. The optic nerves were immersed in fixative and processed for routine transmission electron microscopy. Among the early alterations typical of axonal degeneration, we observed nerve fibers with focal degeneration of the axoplasmic cytoskeleton, watery degeneration and dark degeneration, the latter being prevalent at 168 hours after crush. Our results point to a gradual disintegration of the axoplasmic cytoskeleton, opposed to the previous view of an "all-or-nothing'' process (Griffin et al 1995). We also report that, due to an unknown mechanism, fibers show either a dark or watery pattern of axonal degeneration, as observed in axon profiles. We also observed fibers undergoing early myelin breakdown in the absence of axonal alterations.

Morphological analysis of interstitial cells in murine epididymis using light microscopy and transmission electron microscopy

2021 ◽  
Vol 123 (6) ◽  
pp. 151761
Author(s):  
Tasuku Hiroshige ◽  
Kei-Ichiro Uemura ◽  
Shingo Hirashima ◽  
Kiyosato Hino ◽  
Akinobu Togo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Morphological Analysis ◽  
Interstitial Cells ◽  
Transmission Electron

Morphological Analysis of Particles in Transmission Electron Microscopy Image Using Image Processing

2013 ◽  
Vol 774-776 ◽  
pp. 1262-1266
Author(s):  
Manop Phankokkruad ◽  
Wacharawichanant Sirirat
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Transmission Electron Microscopy ◽  
Morphological Analysis ◽  
Transmission Electron Microscopy Image ◽  
Processing Method ◽  
New Materials ◽  
Manual Method ◽  
Transmission Electron ◽  
Microscopy Image

The analysis of material particles in the transmission electron microscopy (TEM) image is the most important in the development and synthesis of the new materials for application in many fields. Manual identification is the hard work, spent a lot of time and inaccurate method. To solve this problem, the image processing is proposed for automatically identifies the material particles boundary and size in TEM images in or order to extract the useful data. The image processing method has been experimented on variety of TEM images and very promising results have been achieved given more accuracy. Experimental results show that the proposed method gave the high adaptability, more accurate and rapidly than the manual method.

Chemical and Morphological Analysis of Sol-derived Kca2Nb3O10

1999 ◽  
Vol 14 (4) ◽  
pp. 1325-1328 ◽  
Author(s):  
Steven T. Kim ◽  
Vinayak P. Dravid ◽  
Sankar Sambasivan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Morphological Analysis ◽  
Ceramic Matrix Composites ◽  
Ceramic Matrix ◽  
Morphological Properties ◽  
Layered Perovskite ◽  
Matrix Composites ◽  
Fiber Coating ◽  
Transmission Electron

The chemical and morphological properties of a sol-derived layered perovskite compound, Kca2Nb3O10 (KCN), are presented. Development of this compound is motivated by its use as an interphase fiber-coating material for ceramic matrix composites (CMC's). In such systems, this material is to be placed between the fiber and matrix to control crack propagation in the vicinity of the fiber, thereby enhancing toughness. Comparative analyses are performed between known bulk specimens of KCN and the sol-derived product using transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). The suitability of the sol-derived KCN for CMC applications is demonstrated through microstructure and chemical composition similar to that of the known bulk KCN samples.

EM Study of Isopod Hemocytes

1998 ◽  
Vol 4 (S2) ◽  
pp. 1138-1139
Author(s):  
G. M. Vernon ◽  
E. J. Rappa ◽  
W. C. Murray ◽  
R. Witkus
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Morphological Analysis ◽  
Standard Technique ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Small Granule ◽  
Thin Sections ◽  
Cytoplasmic Granules ◽  
Transmission Electron

Crustacean hemocytes have been characterized on the basis of cell size and nature of cytoplasmic granules. Based on light microscopic morphological analysis and cytochemistry, investigators variously named the hemocyte types (agranular, small-granule, large granule, undifferentiated, hyaline cells, non-explosive, explosive granulocytes, etc.). In his study of the isopod Armadillidium vulgare Faso adopted the terminology of Benjamin and James and referred to the hemocytes as hyaline cells, semi-granulocytes and granulocytes.In the present investigation we have studied the hemocytes of two isopods, Oniscus asellus and Armadillidium nasatum, using transmission electron microscopy. Hemolymph was collected by penetrating the posterior dorsal exoskeleton of 20 animals of each genus with a microcapillary pipette and drawing 3-5μL per isopod. The samples were processed following a standard technique. Thin sections were collected on 300 mesh copper grids, counterstained with 2% aqueous uranyl acetate and lead citrate, and viewed with a JEOL 1010 electron microscope.

scholarly journals Association between Caveolin-1 expression and pathophysiological progression of femoral nerves in diabetic foot amputation patients

2017 ◽  
Vol 12 (1) ◽  
pp. 308-314
Author(s):  
Min Ding ◽  
Xianjun Zhu ◽  
Lei Ye ◽  
Meijun Wang ◽  
Yuantao Cui ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Diabetic Foot ◽  
Metabolic Diseases ◽  
Nerve Fibers ◽  
Key Factors ◽  
Fiber Density ◽  
Caveolin 1 ◽  
Transmission Electron ◽  
Clinical Scoring

AbstractObjectiveTo evaluate the pathological changes of femoral nerves and the levels of caveolin-1 in diabetic foot amputation patients with neuropathy, and evaluate the association between caveolin-1 and neuropathy development.MethodThirty seven diabetic foot amputation patients were consecutively recruited from inpatients of Tianjin Metabolic Diseases Hospital between Jan 2003 and Nov 2005. Symptoms and signs of neuropathy, and scores of Toronto Clinical Scoring System (TCSS) were recorded. The nerve conduction velocity and HbA1c were measured. Femoral nerves were obtained 2-3 minutes after amputation. HE, Masson staining and transmission electron microscopy were used for pathological observation. Immunohistochemistry was used to observe changes of axons and count of nerve fiber density (NFD) and detect the levels of caveolin-1.ResultsHE, Masson and transmission electron microscopy showed nerve fibers were asymmetrical, the degenerated axons part had stronger staining and typical demyelinating changes. Stepwise regression models showed that HbA1c and NFD were the independent factors of caveolin-1 (F=45.090, p<0.001, R2=0.790) expression, and Caveolin-1, diabetes duration were independent factors of NFD (F=27.911, p<0.001, R2=0.691).ConclusionCaveolin-1 may be one of the key factors related to pathophysiological progression of femoral nerves in diabetic foot amputation patients.

Preparation of Cellulose Nanocrystal Aerogel from Wastepaper through Freeze-Drying Technique

2015 ◽  
Vol 1125 ◽  
pp. 296-300 ◽  
Author(s):  
Wan Hazman Danial ◽  
Zaiton Abdul Majid ◽  
Mohd Nazlan Mohd Muhid ◽  
Mohd Bakri Bakar ◽  
Zainab Ramli ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Scientific Community ◽  
Freeze Drying ◽  
Morphological Analysis ◽  
Transmission Electron ◽  
Wide Range ◽  
Cellulose Nanomaterials ◽  
Scanning Electron

Cellulose nanomaterials processing for aerogel preparation has received considerable attention among the scientific community due to its fascinating properties. In this work, we report on the preparation of cellulose nanocrystals (CNCs) aerogel from wastepaper using a freeze-drying technique. Structural analysis of the cellulosic particles extracted was investigated by Fourier Transform Infrared Spectroscopy (FTIR). Morphological analysis of the extracted cellulose and CNCs were carried out by Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) respectively. We achieved density of aerogel down to 0.012 g/cm3 which is comparable with typical values of cellulosic aerogels. The preparation of the CNCs aerogel might offers a wide range of aerogel applications through an environmentally friendly conversion of wastepaper material.

scholarly journals Cell morphological analysis of SARS-CoV-2 infection by transmission electron microscopy

2020 ◽  
Vol 12 (8) ◽  
pp. 4368-4373
Author(s):  
Jin Zhao ◽  
Hongxia Zhou ◽  
Wenjing Huang ◽  
Jinchao Zhou ◽  
Minshan Qiu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Morphological Analysis ◽  
Transmission Electron

scholarly journals Correction: Nano-scale morphological analysis of graphene–rubber composites using 3D transmission electron microscopy

RSC Advances ◽  
2014 ◽  
Vol 4 (101) ◽  
pp. 58083-58083
Author(s):  
Amit Das ◽  
Regine Boldt ◽  
René Jurk ◽  
Dieter Jehnichen ◽  
Dieter Fischer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Morphological Analysis ◽  
Rubber Composites ◽  
Nano Scale ◽  
Transmission Electron
Sign in / Sign up

Export Citation Format

Share Document