Alzheimer's disease in Brazilian elderly has a relation with homocysteine but not with MTHFR polymorphisms

OBJECTIVE: To investigate the association between total plasma homocysteine concentration, C677T and A1298C polymorphisms in MTHFR gene and Alzheimer's disease (AD) development. METHOD: Forty-three patients with probable (63%) and possible (37%) AD and 50 non-demented controls were evaluated. Groups did not differ as to gender, age, scholar years, diabetes, alcohol and coffee intake and physical activity. Total plasma homocysteine (Hcy) levels were determined by HPLC and genotyping for MTHFR by PCR/RFLP. Mann-Whitney "U" test was used to compare quantitative variable, Fisher-Freeman-Halton test to compare genotypes and allele proportions and Chi-square test to other qualitative variables. RESULTS: AD patients presented higher total plasma Hcy levels than controls and the difference was statistically significant. No differences in the C677T and A1298C MTHFR polymorphisms distributions were found between patients and controls. Plasma homocysteine concentration did not change with MTHFR genotypes. CONCLUSION: Our data confirms the association between increased plasma Hcy concentration and AD and suggests that neither C677T nor A1298C MTHFR polymorphisms contributed to genetic susceptibility for AD in elderly individuals in the Northeast of Brazil.

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Pro-Inflammatory Cytokines IL-1β and TNF-α are not Associated with Plasma Homocysteine Concentration in Alzheimer’s Disease

Current Alzheimer Research ◽

10.2174/1567205011310020007 ◽

2013 ◽

Vol 10 (2) ◽

pp. 174-179 ◽

Cited By ~ 3

Author(s):

Leon Veryard ◽

Emma Jones ◽

Gary Weaving ◽

Edward Smith ◽

Liz Cheek ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Inflammatory Cytokines ◽

Plasma Homocysteine ◽

Homocysteine Concentration ◽

Plasma Homocysteine Concentration ◽

Tnf Α ◽

Pro Inflammatory Cytokines

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Plasma homocysteine concentration relates to the severity but not to the duration of Alzheimer's disease

International Journal of Geriatric Psychiatry ◽

10.1002/gps.1140 ◽

2004 ◽

Vol 19 (7) ◽

pp. 666-672 ◽

Cited By ~ 12

Author(s):

Karin Nilsson ◽

Lars Gustafson ◽

Björn Hultberg

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Plasma Homocysteine ◽

Homocysteine Concentration ◽

Plasma Homocysteine Concentration

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Is MTHFR polymorphism a risk factor for Alzheimer's disease like APOE?

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2005000100001 ◽

2005 ◽

Vol 63 (1) ◽

pp. 1-6 ◽

Cited By ~ 22

Author(s):

Liana Lisboa Fernandez ◽

Rosane Machado Scheibe

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Risk Factor ◽

Methylenetetrahydrofolate Reductase ◽

Venous Blood ◽

Dna Amplification ◽

Mthfr Gene ◽

Chi Square ◽

Mthfr Polymorphism ◽

Significant Difference

BACKGROUND: The role of methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms as risk factors for the occurence of Alzheimer's disease (AD) is still controversial: OBJECTIVE: To verify the association between MTHFR and apolipoprotein E (APOE) polymorphisms and Alzheimer's disease. METHOD: This work was conducted as a case-control study. Cases included thirty patients with probable AD. Controls were constituted by 29 individuals without dementia according to neuropsychological tests paired to age, sex, race and educational level. DNA was isolated from peripheral leukocytes of anticoagulated venous blood. Genotyping of APOE and MTHFR were performed by DNA amplification and digestion. The frequences of APOE and MTHFR genotypes were submitted by chi-square test corrected by Fisher test; the APOE genotypes, to chi-square linear tendency test and the frequences of MTHFR mutant and AD, by stratificated anlysis adjust by Mantel-Haenszel method. RESULTS: There was significant difference about APOE4 and APOE2 in the groups. (p=0.002) The odds ratio increased exponentially with the increased number of E4 allele (chi2 linear tendency test). No significant difference was detected on MTHFR genotypes in both case and control groups. CONCLUSION: The APOE4 is a risk factor and demonstrated a dose-depenent effect while APOE2 allele conferred a protection to AD. The MTHFR mutation had no correlation with AD.

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