scholarly journals Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

Revista CERES ◽  
2014 ◽  
Vol 61 (3) ◽  
pp. 414-423
Author(s):  
Lílian Estrela Borges Baldotto ◽  
Marihus Altoé Baldotto ◽  
Fábio Lopes Olivares ◽  
Adriane Nunes de Souza
Keyword(s):  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Diazotrophic Bacteria ◽  
Rock Phosphates ◽  
Combined Application ◽  
Phosphate Solubilizing ◽  
Biological Nitrogen

Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i) to select and characterize diazotrophs able to solubilize phosphates in vitro and (ii) evaluate the initial performance of the pineapple cultivars Imperial and Pérola in response to inoculation with selected bacteria in combination with rock phosphate. The experiments were conducted at Universidade Estadual do Norte Fluminense Darcy Ribeiro, in 2009. In the treatments with bacteria the leaf contents of N, P and K were higher than those of the controls, followed by an increase in plant growth. These results indicate that the combined application of diazotrophic phosphate-solubilizing bacteria Burkholderia together with Araxá rock phosphate can be used to improve the initial performance of pineapple slips.

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

scholarly journals Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

2010 ◽  
Vol 12 (3,4) ◽  
pp. 231 ◽  
Author(s):  
M. Ogut ◽  
F. Er ◽  
N. Kandemir
Keyword(s):  
Phosphate Solubilization ◽  
Soil Phosphorus ◽  
Soil Samples ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Batch Cultures ◽  
Phosphate Solubilizers ◽  
Gene Sequence Analysis ◽  
Initial Ph ◽  
Phosphate Solubilizing

<p>Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, <em>Acinetobacter</em> (7), <em>Pseudomonas</em> (7), <em>Enterobacter</em> (2), <em>Enterococcus</em> (1), <em>Escherichia</em> (1), <em>Photorhabdus</em> (1), and <em>Bacillus</em> (1) as determined by the 16S rDNA gene sequence analysis. Since the <em>Acinetobacter</em> species were most effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8  dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. <em>Acinetobacter</em> isolates have some potential as an inoculum both for soil and biotechnological P-solubilization.</p>

scholarly journals Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria

2021 ◽  
Vol 9 (9) ◽  
pp. 1796
Author(s):  
Anna Hegyi ◽  
Tran Bao Khuyen Nguyen ◽  
Katalin Posta
Keyword(s):  
Bacterial Communities ◽  
Phosphate Solubilization ◽  
Negative Relationship ◽  
Chemical Properties ◽  
Rrna Gene ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Phyla ◽  
Physico Chemical ◽  
Phosphate Solubilizing

Bacterial communities can promote increased phosphorus (P) availability for plants and microbes in soil via various mechanisms of phosphate solubilization. The production of extracellular phosphatases releases available P through the hydrolysis of organic P. Examining the abundance and diversity of the bacterial community, including phosphate solubilizing bacteria in soil, may provide valuable information to overcome P scarcity in soil ecosystems. Here, the diversity and relative abundance of bacterial phyla and genera of six agricultural soil samples from Vietnam were analysed by next generation sequencing of the 16S rRNA gene. Phosphatase activities of each soil were compared with physico-chemical parameters and the abundance of the alkaline phosphatase gene phoD. We showed the dominance of Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes. Total nitrogen positively correlated with phyla Proteobacteria, Acidobacteria, Firmicutes and Planctomycetes. The abundance of several genera of Proteobacteria showed positive relationship with the copy number of the phoD gene. The abundance of several taxa positively correlated with silt content, while a negative relationship of Proteobacteria was found with sand content. Our results demonstrated the clear influence of soil physico-chemical properties on the abundance of various bacterial taxa including those potentially involved in phosphate solubilization.

scholarly journals Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils

2013 ◽  
Vol 17 (3) ◽  
pp. 245-252
Author(s):  
Arie Mudjiharjati ◽  
Tri Candra Setiawati ◽  
Martinus Harsanto Pandutama
Keyword(s):  
Organic Matter ◽  
Rock Phosphate ◽  
Available P ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Soil Matrix ◽  
Natural Materials ◽  
Residual P ◽  
Phosphate Solubilizing ◽  
P Absorption

Phosphorous (P) contents in many soils are varies highly along with their ability to provide available P to plant growth. Soils may contain very high in total P, but low in available P due to high P adsorbed by soil matrix and all their adsorbing agents. This research which using natural materials was aimed to increase P availability in some high-P absorption soils. The natural materials utilized for extracting P were organic matter, P extracting bacterial, and rock phosphate. Those materials were interacted to high P absorption soils which were: Oxisol, Inceptisol, and Andisol. The detail objectives of this research were: (1) to study the potential of P-extracting agents (organic matter, and P-extracting bacteria) in releasing P of three high P- absorption soils; (2) to quantify the -age of P coming from the treatments; (3) to calculate the efficiency of P utilization by plant; and (4) to quantify Residual P in soils. The P mobility was analyzed by radioisotope technique using KH232PO4 carrier free solution. The results showed that adding soil organic matter increased the available P by 15.24% in Oxisol, 40.18 in Inceptisol, and by 7.34% in Andisol. Plant sorption toward P from % P used efficiency(%-PUE) up to 60 days was still very low, 0.65 to 9.34%. This was in accordance with the residual P in soils which were still quite high ranging from 94% to 96% in Andisol, 91%97% in Inceptisol, and 96%-98% in Oxisol. The implication of the results of this research, however, is that the application of natural materials in improving soil P availability provides a longtimeresidual effect which could give benefit to the following crops.Keywords: Andisols; Inceptisol; Oxisols; P. diminuta; PUE;  rock phosphate[How to Cite: Mudjiharjati A, TC Setiawati and MH Pandutama. 2012. Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils. J Trop Soils, 17 (3) : 245-252. doi: 10.5400/jts.2012.17.3.245][Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.3.245]

scholarly journals Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

2021 ◽  
Vol 11 ◽  
Author(s):  
Ubiana C. Silva ◽  
Sara Cuadros-Orellana ◽  
Daliane R. C. Silva ◽  
Luiz F. Freitas-Júnior ◽  
Ana C. Fernandes ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Genomic Analysis ◽  
Iron Phosphate ◽  
Acid Synthesis ◽  
Phosphate Solubilizing Bacteria ◽  
P Sources

Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe–P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth.

scholarly journals Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem

2020 ◽  
Vol 5 (1) ◽  
pp. 300-304
Author(s):  
Betty Natalie Fitriatin ◽  
Dita Fauziah ◽  
Fabira Nur Fitriani ◽  
Dewi Nurma Ningtyas ◽  
Pujawati Suryatmana ◽  
...  
Keyword(s):  
Acid Soil ◽  
Block Design ◽  
Maize Seedling ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Soil Ecosystem ◽  
Maize Productivity ◽  
Randomized Block Design ◽  
Phosphate Solubilizing ◽  
Microbial Fertilizers

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

2021 ◽  
Vol 12 ◽  
Author(s):  
Hassan Etesami ◽  
Byoung Ryong Jeong ◽  
Bernard R. Glick
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Crop Production ◽  
Current Knowledge ◽  
Arbuscular Mycorrhizal ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

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