Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria

Bacterial communities can promote increased phosphorus (P) availability for plants and microbes in soil via various mechanisms of phosphate solubilization. The production of extracellular phosphatases releases available P through the hydrolysis of organic P. Examining the abundance and diversity of the bacterial community, including phosphate solubilizing bacteria in soil, may provide valuable information to overcome P scarcity in soil ecosystems. Here, the diversity and relative abundance of bacterial phyla and genera of six agricultural soil samples from Vietnam were analysed by next generation sequencing of the 16S rRNA gene. Phosphatase activities of each soil were compared with physico-chemical parameters and the abundance of the alkaline phosphatase gene phoD. We showed the dominance of Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes. Total nitrogen positively correlated with phyla Proteobacteria, Acidobacteria, Firmicutes and Planctomycetes. The abundance of several genera of Proteobacteria showed positive relationship with the copy number of the phoD gene. The abundance of several taxa positively correlated with silt content, while a negative relationship of Proteobacteria was found with sand content. Our results demonstrated the clear influence of soil physico-chemical properties on the abundance of various bacterial taxa including those potentially involved in phosphate solubilization.

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Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

Eurasian Chemico-Technological Journal ◽

10.18321/ectj50 ◽

2010 ◽

Vol 12 (3,4) ◽

pp. 231 ◽

Cited By ~ 1

Author(s):

M. Ogut ◽

F. Er ◽

N. Kandemir

Keyword(s):

Phosphate Solubilization ◽

Soil Phosphorus ◽

Soil Samples ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Batch Cultures ◽

Phosphate Solubilizers ◽

Gene Sequence Analysis ◽

Initial Ph ◽

Phosphate Solubilizing

Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, Acinetobacter (7), Pseudomonas (7), Enterobacter (2), Enterococcus (1), Escherichia (1), Photorhabdus (1), and Bacillus (1) as determined by the 16S rDNA gene sequence analysis. Since the Acinetobacter species were most effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8 dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. Acinetobacter isolates have some potential as an inoculum both for soil and biotechnological P-solubilization.

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Physico-chemical properties and associated active microbes of tea garden waste of Golaghat district of Assam, India

FLORA AND FAUNA ◽

10.33451/florafauna.v26i2pp247-253 ◽

2020 ◽

Vol 26 (2) ◽

Author(s):

Apurba Saikia ◽

Pavitra Chutia ◽

Satya Ranjan Sarmah

Keyword(s):

Chemical Properties ◽

Value Added ◽

Soluble Solids ◽

Waste Materials ◽

Phosphate Solubilizing Bacteria ◽

Tea Plantation ◽

Physico Chemical ◽

Phosphate Solubilizing ◽

Tea Plants ◽

Natural Productivity

Tea Gardens generally produce two kinds of waste materials such as Tea Factory waste (TFW) and Pruning Litter (PL). Minimum 2 % of TFW is produced as by - product during processing of tea contributing a huge quantity as waste material.Similarly during pruning operations considerable amount of biomass are produced. Both the bio-waste materials can be managed by biological process to produce value added organic supplement to enrich the soil of tea plantation. During the present study different physico-chemical properties, viz. pH, P2O2 , K2O and nitrogen’s of TFW were analyzed. In TFW the value of Total soluble solids ranged (27.2-31.1%) and Crude fiber ranged (25.83-31.12%) and Caffenine amount ranged (23.14- 27.94%) and Total ash ranged (3.15-6.54%) The aflavin ranged (0.45-0.76%) and the arubignins ranged (18.45-21.32%). In TFW and PL some beneficial and active microbial populations are found which can be used in tea plants after making value added compost to increase their natural productivity. In this study focus was given to explore the beneficial and active microorganisms found in TFW and PL and found some beneficial microbes such as Actinomycetes, Azotobacter, Azospirillum and Phosphate solubilizing bacteria respectively

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Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

Revista CERES ◽

10.1590/s0034-737x2014000300017 ◽

2014 ◽

Vol 61 (3) ◽

pp. 414-423

Author(s):

Lílian Estrela Borges Baldotto ◽

Marihus Altoé Baldotto ◽

Fábio Lopes Olivares ◽

Adriane Nunes de Souza

Keyword(s):

Rock Phosphate ◽

Phosphate Solubilization ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Diazotrophic Bacteria ◽

Rock Phosphates ◽

Combined Application ◽

Phosphate Solubilizing ◽

Biological Nitrogen

Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i) to select and characterize diazotrophs able to solubilize phosphates in vitro and (ii) evaluate the initial performance of the pineapple cultivars Imperial and Pérola in response to inoculation with selected bacteria in combination with rock phosphate. The experiments were conducted at Universidade Estadual do Norte Fluminense Darcy Ribeiro, in 2009. In the treatments with bacteria the leaf contents of N, P and K were higher than those of the controls, followed by an increase in plant growth. These results indicate that the combined application of diazotrophic phosphate-solubilizing bacteria Burkholderia together with Araxá rock phosphate can be used to improve the initial performance of pineapple slips.

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Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem

Open Agriculture ◽

10.1515/opag-2020-0036 ◽

2020 ◽

Vol 5 (1) ◽

pp. 300-304

Author(s):

Betty Natalie Fitriatin ◽

Dita Fauziah ◽

Fabira Nur Fitriani ◽

Dewi Nurma Ningtyas ◽

Pujawati Suryatmana ◽

...

Keyword(s):

Acid Soil ◽

Block Design ◽

Maize Seedling ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Soil Ecosystem ◽

Maize Productivity ◽

Randomized Block Design ◽

Phosphate Solubilizing ◽

Microbial Fertilizers

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.

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ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

African Journal of Health, Safety and Environment ◽

10.52417/ajhse.v1i2.83 ◽

2020 ◽

Vol 1 (2) ◽

pp. 37-51

Author(s):

C. E. Oshoma ◽

S. O. Nwodo ◽

I. S. Obuekwe

Keyword(s):

Acid Phosphatase ◽

Contaminated Soil ◽

Rock Phosphate ◽

Phosphate Solubilization ◽

Enzymatic Activities ◽

Available Phosphorus ◽

Phosphate Solubilizing Bacteria ◽

Indigenous Bacteria ◽

Rock Phosphate Solubilization ◽

Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

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Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

Iraqi Journal of Science ◽

10.24996/ijs.2019.60.5.7 ◽

2019 ◽

Vol 60 (5) ◽

pp. 985-995

Author(s):

Yusur Ramzi ◽

Hutaf A. A. Alsalim

Keyword(s):

Plant Growth ◽

Bacillus Cereus ◽

Mung Bean ◽

Phosphate Solubilization ◽

Growth Promotion ◽

Hydrolytic Enzymes ◽

Phosphate Solubilizing Bacteria ◽

Biochemical Tests ◽

Isolation And Identification ◽

Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 Î¼g.ml-1), Y4 was the highest (39.00 Î¼g.ml-1) followed by Y3 (37.00Î¼g.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

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Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

Frontiers in Plant Science ◽

10.3389/fpls.2021.699618 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hassan Etesami ◽

Byoung Ryong Jeong ◽

Bernard R. Glick

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Crop Production ◽

Current Knowledge ◽

Arbuscular Mycorrhizal ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

P Deficiency ◽

Phosphate Solubilizing ◽

P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

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Seed bio-priming with phosphate solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions

10.1101/2021.11.23.469751 ◽

2021 ◽

Author(s):

Musa Ibrahim Saheed ◽

Beckley Ibrahim Ikhajiagbe

Keyword(s):

Bacillus Cereus ◽

Proteus Mirabilis ◽

Rice Plant ◽

Bacterial Colonization ◽

Rrna Gene ◽

Rice Seed ◽

Phosphate Solubilizing Bacteria ◽

Growth Properties ◽

Rice Seeds ◽

Phosphate Solubilizing

The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growthpromoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through bio-priming strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. Biosafety analysis of the PSB isolates was conducted using blood agar. The rice seeds were then bio-primed with the PSBs at 3, 12 and 24 hours priming durations and then sown in a composite FU soil sample. Differences in germination bioassay involving SEM, morphology, physiology and biomass parameters were investigated for 15 weeks after bio-priming. The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds without bio-priming in FU soil. Germination parameters was better in seeds bio-primed with the PSBs, especially at 12h priming duration as against seeds without priming. SEM showed more bacterial colonization in the PSB bioprimed seeds. Seed bio-priming of rice seed with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved seed microbiome, rhizocolonization and soil nutrient properties, thereby enhancing growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability and soil property for optimum plant usage in phosphate stressed and iron toxic soils.

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Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

Polish Journal of Microbiology ◽

10.5604/17331331.1234993 ◽

2017 ◽

Vol 66 (1) ◽

pp. 57-65 ◽

Cited By ~ 19

Author(s):

Jian Zhang ◽

Peng Cheng Wang ◽

Ling Fang ◽

Qi-An Zhang ◽

Cong Sheng Yan ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Wood Flour ◽

Dry Weight ◽

Rrna Gene ◽

Plant Growth Promoting Bacteria ◽

Phosphate Solubilizing Bacteria ◽

Sequence Comparisons ◽

Isolation And Characterization ◽

Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

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Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Agriculture ◽

10.3390/agriculture10090383 ◽

2020 ◽

Vol 10 (9) ◽

pp. 383 ◽

Cited By ~ 3

Author(s):

Gustavo Enrique Mendoza-Arroyo ◽

Manuel Jesús Chan-Bacab ◽

Ruth Noemi Aguila-Ramírez ◽

Benjamín Otto Ortega-Morales ◽

René Efraín Canché Solís ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Inorganic Phosphate ◽

Extracellular Polymeric Substances ◽

Phosphate Solubilization ◽

Rrna Gene ◽

Bacterial Strains ◽

Habanero Pepper ◽

Phosphate Solubilizing

The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

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