Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils

Phosphorous (P) contents in many soils are varies highly along with their ability to provide available P to plant growth. Soils may contain very high in total P, but low in available P due to high P adsorbed by soil matrix and all their adsorbing agents. This research which using natural materials was aimed to increase P availability in some high-P absorption soils. The natural materials utilized for extracting P were organic matter, P extracting bacterial, and rock phosphate. Those materials were interacted to high P absorption soils which were: Oxisol, Inceptisol, and Andisol. The detail objectives of this research were: (1) to study the potential of P-extracting agents (organic matter, and P-extracting bacteria) in releasing P of three high P- absorption soils; (2) to quantify the -age of P coming from the treatments; (3) to calculate the efficiency of P utilization by plant; and (4) to quantify Residual P in soils. The P mobility was analyzed by radioisotope technique using KH232PO4 carrier free solution. The results showed that adding soil organic matter increased the available P by 15.24% in Oxisol, 40.18 in Inceptisol, and by 7.34% in Andisol. Plant sorption toward P from % P used efficiency(%-PUE) up to 60 days was still very low, 0.65 to 9.34%. This was in accordance with the residual P in soils which were still quite high ranging from 94% to 96% in Andisol, 91%97% in Inceptisol, and 96%-98% in Oxisol. The implication of the results of this research, however, is that the application of natural materials in improving soil P availability provides a longtimeresidual effect which could give benefit to the following crops.Keywords: Andisols; Inceptisol; Oxisols; P. diminuta; PUE; rock phosphate[How to Cite: Mudjiharjati A, TC Setiawati and MH Pandutama. 2012. Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils. J Trop Soils, 17 (3) : 245-252. doi: 10.5400/jts.2012.17.3.245][Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.3.245]

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Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N2-Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16132442 ◽

2019 ◽

Vol 16 (13) ◽

pp. 2442 ◽

Cited By ~ 1

Author(s):

Zhikang Wang ◽

Ziyun Chen ◽

Xiangxiang Fu

Keyword(s):

Plant Growth ◽

Soil Properties ◽

Soil Amendment ◽

Growth Promotion ◽

Synergistic Effects ◽

Available P ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Unsterilized Soil ◽

Phosphate Solubilizing

The inoculation of beneficial microorganisms to improve plant growth and soil properties is a promising strategy in the soil amendment. However, the effects of co-inoculation with phosphate-solubilizing bacteria (PSB) and N2-fixing bacteria (NFB) on the soil properties of typical C-deficient soil remain unclear. Based on a controlled experiment and a pot experiment, we examined the effects of PSB (M: Bacillus megaterium and F: Pseudomonas fluorescens), NFB (C: Azotobacter chroococcum and B: Azospirillum brasilence), and combined PSB and NFB treatments on C, N, P availability, and enzyme activities in sterilized soil, as well as the growth of Cyclocarya Paliurus seedlings grow in unsterilized soil. During a 60-day culture, prominent increases in soil inorganic N and available P contents were detected after bacteria additions. Three patterns were observed for different additions according to the dynamic bacterial growth. Synergistic effects between NFB and PSB were obvious, co-inoculations with NFB enhanced the accumulation of available P. However, decreases in soil available P and N were observed on the 60th day, which was induced by the decreases in bacterial quantities under C deficiency. Besides, co-inoculations with PSB and NFB resulted in greater performance in plant growth promotion. Aimed at amending soil with a C supply shortage, combined PSB and NFB treatments are more appropriate for practical fertilization at intervals of 30–45 days. The results demonstrate that co-inoculations could have synergistic interactions during culture and application, which may help with understanding the possible mechanism of soil amendment driven by microorganisms under C deficiency, thereby providing an alternative option for amending such soil.

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Production of phospho-vermicompost by earthworms mediated bio-conversion of organic residues and rock phosphate

Progressive Agriculture ◽

10.3329/pa.v31i3.52124 ◽

2021 ◽

Vol 31 (3) ◽

pp. 195-204

Author(s):

MA Haque ◽

MM Ali ◽

MSH Bhuiyan

Keyword(s):

Rice Straw ◽

Water Hyacinth ◽

Rock Phosphate ◽

Available P ◽

Phosphate Solubilizing Bacteria ◽

Organic Residues ◽

Chemical Fertilizers ◽

Total N ◽

Phosphate Solubilizing ◽

Total Bacteria

A glass house experiment was conducted to develop phospho-vermicompost using different combination of organic residues amended with rock phosphate and earthworms at the Soil Science Division, BINA, Mymensingh. The experiment was conducted in a Completely Randomized Design with eight treatments and three replications. The treatments were as T1:50% Cowdung (CD) + 50% Mustard straw (MST), T2:50% CD + 50% Water hyacinth (WH), T3:50% CD + 50% Rice straw (RST), T4:50% CD + 25% WH + 25% RST, T5:50% CD + 50% MST + 4% Rock phosphate (RP), T6:50% CD + 50% WH + 4% RP, T7:50% CD + 50% RST + 4% RP, T8:50% CD + 25% WH + 25% RST + 4% RP. About 150 earth worms (Eisenia foetida or Red wiggler earthworms) were released on partial decomposed residues into the pots. At the end of the incubation, population of earthworms, total bacteria and phosphate solubilizing bacteria were determined from prepared vermicompost. pH, organic carbon, total N, P, K, S, available P and alkaline phosphatase activity were also determined from prepared vermicomposts. pH was found almost similar in all the treatment combinations but with rock phosphate amended treatments showed greater pH than without rock phosphate amended treatments. The treatment T8 (50% cowdung +25% water hyacinth +25% rice straw with 4% rock phosphate powder + red wiggler earthworms) showed significantly highest population of earthworms, total bacteria, phosphate solubilizing bacteria (PSB) and alkaline phosphatase activity (ALPA) and gave lower C:N and C:P ratio which might be resulted the higher amount of nutrients including available P in mature vermicompost. However, among the treatments, the treatments T8 gave the highest total N (1.42%), P (1.45%), K (1.52%) and S (0.35%) and available P contents than that of other treatments which indicated the better quality of phospho-vermicompost. Therefore, 50% cowdung +25% water hyacinth +25% rice straw with 4% rock phosphate powder and red wiggler earthworms could be used for the production of phospho-vermicompost. The developed phospho-vermicompost could be used for supplement of phosphatic fertilizer and other chemical fertilizers in the cultivation of different crops and also could be saved of chemical fertilizers. Progressive Agriculture 31 (3): 195-204, 2020

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Improvement of Soil Chemistry Characteristic of Upland with Rock Phosphate, Phosphate-Solubilizing Bacteria and Farm Yard Manure For Increase of Soybean Yield

Journal of Tropical Soils ◽

10.5400/jts.2008.v13i1.49-58 ◽

2008 ◽

Vol 13 (1) ◽

pp. 49-58

Author(s):

Aidi Noor

Keyword(s):

Grain Yield ◽

Nutrient Uptake ◽

Rock Phosphate ◽

Block Design ◽

Available P ◽

Multiple Regression Equation ◽

Phosphate Solubilizing Bacteria ◽

Farm Yard Manure ◽

Soybean Yield ◽

Phosphate Solubilizing

The experiment aims to know the role of rock phosphate, phosphate-solubilizing bacteria and farm yard manure application in improve quality of acid upland and its influence to soybean yield. Experiment was conducted in the greenhouse using Ultisols from Kentrong at 2001 year. Factorial experiment design with two factors was used in randomized complete block design with three replications. The first factors was rock phosphate dosages i.e. : 0; 30; 60; 90 kg P ha-1, and the second factor was combination of phosphate solubilizing bacteria (PSB) and farm yard manure (FYM) i.e. : without PSB and FYM (control); PSB (Pseudomonas fluorescens); FYM 10 t ha-1, and PSB+FYM. Results indicated that rock phosphate and combination of PSB and FYM application increased available P and decrase exchangeable Al of soils, increase nutrient uptake N, P, nodulation and grain yield of soybean. Application of phosphate solubilizing bacteria and farm yard manure combination with rock phosphate 30 kg P ha-1 dosages average increase grain yield soybean 23.5% compared with control. Grain yield of soybean 87% affected by variables of available P soils (X1), nutrient uptake N (X4) and P (X5), with multiple regression equation : Y = 0.241 + 0.03900 X1 + 0.00470 X4 + 0.04263 X5.

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Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

Revista CERES ◽

10.1590/s0034-737x2014000300017 ◽

2014 ◽

Vol 61 (3) ◽

pp. 414-423

Author(s):

Lílian Estrela Borges Baldotto ◽

Marihus Altoé Baldotto ◽

Fábio Lopes Olivares ◽

Adriane Nunes de Souza

Keyword(s):

Rock Phosphate ◽

Phosphate Solubilization ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Diazotrophic Bacteria ◽

Rock Phosphates ◽

Combined Application ◽

Phosphate Solubilizing ◽

Biological Nitrogen

Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i) to select and characterize diazotrophs able to solubilize phosphates in vitro and (ii) evaluate the initial performance of the pineapple cultivars Imperial and Pérola in response to inoculation with selected bacteria in combination with rock phosphate. The experiments were conducted at Universidade Estadual do Norte Fluminense Darcy Ribeiro, in 2009. In the treatments with bacteria the leaf contents of N, P and K were higher than those of the controls, followed by an increase in plant growth. These results indicate that the combined application of diazotrophic phosphate-solubilizing bacteria Burkholderia together with Araxá rock phosphate can be used to improve the initial performance of pineapple slips.

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Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem

Open Agriculture ◽

10.1515/opag-2020-0036 ◽

2020 ◽

Vol 5 (1) ◽

pp. 300-304

Author(s):

Betty Natalie Fitriatin ◽

Dita Fauziah ◽

Fabira Nur Fitriani ◽

Dewi Nurma Ningtyas ◽

Pujawati Suryatmana ◽

...

Keyword(s):

Acid Soil ◽

Block Design ◽

Maize Seedling ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Soil Ecosystem ◽

Maize Productivity ◽

Randomized Block Design ◽

Phosphate Solubilizing ◽

Microbial Fertilizers

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.

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ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

African Journal of Health, Safety and Environment ◽

10.52417/ajhse.v1i2.83 ◽

2020 ◽

Vol 1 (2) ◽

pp. 37-51

Author(s):

C. E. Oshoma ◽

S. O. Nwodo ◽

I. S. Obuekwe

Keyword(s):

Acid Phosphatase ◽

Contaminated Soil ◽

Rock Phosphate ◽

Phosphate Solubilization ◽

Enzymatic Activities ◽

Available Phosphorus ◽

Phosphate Solubilizing Bacteria ◽

Indigenous Bacteria ◽

Rock Phosphate Solubilization ◽

Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

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Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

Frontiers in Plant Science ◽

10.3389/fpls.2021.699618 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hassan Etesami ◽

Byoung Ryong Jeong ◽

Bernard R. Glick

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Crop Production ◽

Current Knowledge ◽

Arbuscular Mycorrhizal ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

P Deficiency ◽

Phosphate Solubilizing ◽

P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

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