Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.

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Manajemen Pemupukan N-P-K dengan Pupuk Hayati untuk Mempertahankan Kandungan N dan P Tanah serta Hasil Kedelai

Jurnal Agro ◽

10.15575/1201 ◽

2017 ◽

Vol 4 (1) ◽

pp. 15-23

Author(s):

Emma Trinurani Sofyan ◽

Stefina Liana Sari

Keyword(s):

Soil Microorganisms ◽

Block Design ◽

Inorganic Fertilizer ◽

Soybean Plant ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Nitrogen Fixing Bacteria ◽

Available Nutrients ◽

Randomized Block Design ◽

Phosphate Solubilizing

One effort to increase the productivity of soybeans in Inceptisols Jatinangor is through utilize inoculant of soil microorganisms such as nitrogen-fixing bacteria and phosphate-solubilizing bacteria in the form of specific biofertilizer (SB) for soybean plant without leaving inorganic fertilizer as a source of available nutrients to plants. This research aimed to determine fertilization management of N-P-K and specific biofertilizer in retaining N and P availability and yield of soybean. The Research was conducted from April to July 2016, arranged in Simple Randomized Block Design (RBD) and consisted of 10 treatments. The combination of biofertilizer and N-P-K were as follows; A = control (without fertilizer), B = Standard N-P-K, C = 0 N-P-K + 1 SB , D = 1/4 N-P-K + 1 SB, E = 1/2 N-P-K + 1 SB, F = 3/4 N-P-K + 1 SB, G = 1 N-P-K + 1 SB, H = 3/4 + 1/4 SB, I = 3/4 N-P-K + 1/2 SB dan J = 3/4 N-P-K + 3/4 SB. The results showed a dose of N-P-K and biofertilizer was recommended in the treatment E (250 kg ha-1 SB) and 1/2 N-P-K (37.5-50-50 kg ha-1). The dose was able to increase nitrogen and phosphor content in soil and plant, also on yield of soybean up to 0.4 t ha-1 or 32% of a standard N-P-K.

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Uji Aplikasi Bakteri Pelarut Fosfat (BPF) dan Amelioran Organik untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung pada Ultisol

Composite: Jurnal Ilmu Pertanian ◽

10.37577/composite.v2i02.236 ◽

2020 ◽

Vol 2 (02) ◽

pp. 74-81

Author(s):

Fiqriah Hanum Khumairah ◽

Anggi Jingga ◽

Betty Natalie Fitriatin ◽

Tualar Simarmata

Keyword(s):

Block Design ◽

Growth And Yield ◽

Phosphate Solubilizing Bacteria ◽

Maize Productivity ◽

Randomized Block Design ◽

Two Factors ◽

P Fertilizer ◽

Enterobacter Ludwigii ◽

Four Levels ◽

Phosphate Solubilizing

Maize (Zea mays L.) is an important food crop in Indonesia as a source of carbohydrates. The extent of land conversion reduces maize productivity. The experiment aimed to increase the growth and yield of maize in Ultisol which was carried out in the experimental garden of the West Java Horticultural and Various Seed Plant Development Center from October 2017 to March 2018. The Phosphate Solubilizing Bacteria (PSB) and organic ameliorant application test used the factorial randomized block design (RBD) method consisted of two factors. The first factor is PSB and organic ameliorant which consisted of four levels, namely b0 (without the provision of PSB and organic ameliorant), b1 (BP 107 cfu mL-1), b2 (organic ameliorant 5 tons ha-1), and b3 (PSB 107 cfu mL-1 + organic ameliorant 5 tons ha-1) and the second factor is the dosage of SP-36 fertilizer consisted of five levels, namely po (without SP-36 fertilizer), p1 (100% SP-36 fertilizer), p2 (90% SP-36 fertilizer, P3 (80% SP-36 fertilizer), and P4 (70% SP-36 fertilizer). The results showed that the application of PSB (Enterobacter ludwigii + Bulkholderia vietnamiensis + Citrobacter amalonaticus) 107 cfu mL-1 and organic ameliorant dosage of 5 tons ha-1 was able to increase plant height at age 6 Week After Planting (WAP), weight of cob with and without cornhusk, and weight of 100 seeds in the framework of increasing the growth and yield of maize on Ultisols and reduce the use of inorganic P fertilizer to 90%.

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The Application of Phosphate Solubilizing Microbes Biofertilizer to Increase Soil P and Yield of Maize on Ultisols Jatinangor

KnE Life Sciences ◽

10.18502/kls.v2i6.1037 ◽

2017 ◽

Vol 2 (6) ◽

pp. 179

Author(s):

Betty Natalie Fitriatin ◽

Pujawati Suryatmana ◽

Anny Yuniarti ◽

Noor Istifadah

Keyword(s):

Soil Phosphorus ◽

Block Design ◽

Fertilizer Efficiency ◽

Phosphate Solubilizing Bacteria ◽

Soil P ◽

Soil Phosphate ◽

Randomized Block Design ◽

P Fertilizer ◽

Aspergillus Sp ◽

Phosphate Solubilizing

Ultisols has problems of low availability of nutrients, especially phosphorus. To improve soil phosphate and P fertilizer efficiency, it is necessary to develop biofertilizer such as phosphate solubilizing microbes. Phosphate solubilizing microbes (PSM) have the capability of dissolving soil phosphorus which have been adsorbed and can mineralize organic P to become inorganic P, hence increasing the avalibility of P in the soil. Phosphate solubilizing bacteria (Pseudomonas mallei and Pseudomonas cepacea) and phosphate solubilizing fungi (Penicillium sp. and Aspergillus sp) were selected based on their ability to dissolve P. The experiment was conducted at Jatinangor, West Java Indonesia to study the application of PSM biofertilizer to increase soil P and yield of maize. Experiment used a Randomized Block Design (RBD) in factorial pattern, consisting of two factors with three replications. The first factor consisted of PSM biofertilizer, which were; without PSM, 5 L ha-1 of PSM and 50 kg ha-1 of PSM. The second factor was P fertilizer with five levels (0%, 25%, 50%, 75% and 100% dosage of recommendation). The results showed that the application of PSM biofertilizer increased soil phosphate and yield of maize on Ultisol Jatinangor. The dosage of P inorganic fertilizers was reduced by 50%.Keywords: ultisol, maize, biofertillizer, phospate-solubilizing bacteria.

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Populasi Bakteri Pelarut Fosfat, P-Potensial Dan Hasil Jagung Yang Dipengaruhi Oleh Aplikasi MPF Pada Ultisols Jatinangor

Agrologia ◽

10.30598/a.v6i2.171 ◽

2017 ◽

Vol 6 (2) ◽

Author(s):

Betty Natalie Fitriatin ◽

Mayang Agustina ◽

Reginawanti Hindersah

Keyword(s):

Total Population ◽

Zea Mays L ◽

Block Design ◽

Maize Yield ◽

Phosphate Fertilizer ◽

Low Fertility ◽

Phosphate Solubilizing Bacteria ◽

Randomized Block Design ◽

Microbial Group ◽

Phosphate Solubilizing

Ultisol is low fertility soil and as low available P due to P fixation by Al and Fe. The phosphate solubilizing microbes (PSM) is the microbial group that enable realease fixed-P to soil solution and be absorbed by plant. This research was aimed to determine the effects of PSM biofertilizer and phosphate fertilizer dosage combination on soil potential P, total phosphate solubilizing bacteria (PSB) population and yield of maize (Zea mays L.) grown in Ultisols. This research used a Randomized Block Design that consist of nine treatments with three replications. The treatments were 100 kg ha-1 of SP-36 fertilizer, 50 kg ha-1 of PSM biofertilizer, SP-36 fertilizer (50 kg ha-1 and 100 kg ha-1) with PSM biofertilizer (25 kg ha-1, 50 kg ha-1 and 75 kg ha-1) repectively. The result showed that the combination of PSM and phosphate fertilizer dosage had significantly effect on PSB total population and maize yield, but did not not on potential P. This experiment suggested that SP-36 fertilizer with 50 kg ha-1 dosage and PSM biofertilizer with 50 kg ha-1 dosage is the best combination.

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PENGARUH APLIKASI BAKTERI PELARUT FOSFAT DAN PUPUK ANORGANIK FOSFAT TERHADAP POPULASI BAKTERI PELARUT FOSFAT, P-TERSEDIA, DAN HASIL TANAMAN JAGUNG PADA ALFISOL

Jurnal Tanah dan Sumberdaya Lahan ◽

10.21776/ub.jtsl.2021.008.2.15 ◽

2021 ◽

Vol 8 (2) ◽

pp. 437-449

Author(s):

Gertruda Lovitna ◽

Yulia Nuraini ◽

Nurul Istiqomah

Keyword(s):

Crop Yields ◽

Block Design ◽

Chemical Properties ◽

Dry Weight ◽

Phosphate Fertilizer ◽

Corn Yield ◽

Phosphate Solubilizing Bacteria ◽

Randomized Block Design ◽

Insoluble Form ◽

Phosphate Solubilizing

Fulfilment of nutrients for plants can be maximized when fertilization, but the application of inorganic fertilizers can cause damage to the soil if the dosage is excessive. The utilization of phosphate solubilizing bacteria is an effort to increase the efficiency of phosphate fertilization that can extract phosphate from an insoluble form that becomes available. This study aimed to obtain a combination of inorganic fertilization doses (SP-36 and NPK) and the application of biological fertilizers that utilize phosphate solubilizing bacteria (PSB) to increase the population of phosphate solubilizing bacteria and P-available in the soil, as well as corn crop yields. This study used a randomized block design with nine treatments and three replications. The result showed that the application of PSB and inorganic phosphate fertilizer shows a significant effect on the population of PSB, P-available, plant height, stover dry weight, cob weight with husk and cob weight without husk, but didn’t show a significant effect on 100 seeds weight and number of plant leaves. Based on the correlation analysis, the results show a positive correlation between soil chemical properties, P-available, and corn yield.

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Pengaruh Aplikasi Pupuk Hayati Mikroba Pelarut Fosfat dalam Meningkatkan Kandungan P tanah , Pertumbuhan dan Hasil Jagung pada Ultisols

SoilREns ◽

10.24198/soilrens.v14i2.11033 ◽

2017 ◽

Vol 14 (2) ◽

Author(s):

Betty Natalie Fitriatin ◽

Aristyo Rahardiyan ◽

Tien Turmuktini

Keyword(s):

Extracellular Enzymes ◽

Block Design ◽

Fertilizer Application ◽

Growth And Yield ◽

Phosphate Solubilizing Bacteria ◽

Soil P ◽

Randomized Block Design ◽

Aspergillus Sp ◽

Phosphate Solubilizing ◽

Biological Fertilizer

Marginal soils have problems of low availability of nutrients, especially phosphorus. Phosphate solubilizing microbes can produce organic acid that dissolve P in the soil. Futhermore, the PSM also produce extracellular enzymes asa phosphatase that catalyze mineralization of organic P become inorganic P. Phosphate solubilizing bacteria (Pseudomonas mallei and Pseudomonas cepacea) and phosphate solubilizing fungi (Penicillium sp. and Aspergillus sp) is selected based on the ability of dissolving P and the production of growth regulators. Research at this stage aims to determine the biological fertilizer application techniques (PSB and PSF) as well as different types of P fertilizers to improve soil P available, growth and yield of maize. Field experiment on Ultisol Jatinangor implemented using a randomized block design (RBD) to test how applications PSM plus (giving 1, 2 and 3 applications) as well as the type of fertilizer P (SP-36 and rock phosphate). The results showed that the application of PSM can improve soil P-available growth and yield of maize. Application of PSM biological fertilizer could reduce the need for fertilizer P by 50%. PSM application once the application (early planting) with SP-36 dose 50% can increase content of soil P (P-available), and yield of maize on Ultisols.Keywords : maize, phosphate solubilizing microbes, Ultisols

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Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera

Jurnal Teknologi Lingkungan ◽

10.29122/jtl.v21i1.3743 ◽

2020 ◽

Vol 21 (1) ◽

pp. 40-48

Author(s):

Muhammad Asril ◽

Yuni Lisafitri

Keyword(s):

Soil Microbes ◽

Acid Soil ◽

Acid Soils ◽

Soil Samples ◽

Pathogenicity Test ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Potato Tubers ◽

Phosphate Availability ◽

Phosphate Solubilizing

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam

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GROWTH RESPONSE OF VIGNA UNGUICULATA CULTIVATED ON ORGANIC MANURES AND BIOFERTILIZERS

FLORA AND FAUNA ◽

10.33451/florafauna.v23i1pp90-94 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

BHALSHANKAR CHHAYA

Keyword(s):

Organic Nitrogen ◽

Growth Response ◽

Block Design ◽

Organic Fertilizers ◽

Phosphate Solubilizing Bacteria ◽

Organic Manures ◽

Randomized Block Design ◽

Effect On Yield ◽

Phosphate Solubilizing ◽

And Control

Vermicompostis is a method of making compost with the use of earthworms. Neem cake is a seed residue left after extraction of oil used as a biopesticide and organic nitrogen fertilizer. Azotobacter bacteria are used as nitrogen fixing biofertilizer and Phosphate solubilizing bacteria. All these organic fertilizers and biofertilizers are managed to study their effect on yield of crop Vignaunguiculata. Treatments are given as VC, NC, Bio,NPK and control in randomized block design. Highest DM yield was shown inVC followed by NPK, BIO, and NC and lowest in CON.

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Growth, Yield and Quality of Cauliflower as Influenced by Different Sources of Nutrients and Mulching in Indo- Gangatic Plains of Western Uttar Pradesh

International Research Journal of Pure and Applied Chemistry ◽

10.9734/irjpac/2020/v21i2430367 ◽

2020 ◽

pp. 300-307

Author(s):

Amit Kumar ◽

Mukesh Kumar ◽

Bijendra Singh ◽

Manoj Kumar Singh ◽

S. P. Singh ◽

...

Keyword(s):

Block Design ◽

Uttar Pradesh ◽

Growth Yield ◽

Quality Parameters ◽

Phosphate Solubilizing Bacteria ◽

Yield And Quality ◽

Randomized Block Design ◽

Phosphate Solubilizing ◽

And Control ◽

Different Sources

A field experiment was carried out at Horticulture Research Center of Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut, (UP) India during rabi season 2018-19 and 2019-20 respectively with entitled “Effect of different sources of nutrients and mulching on sustainable production of Cauliflower (Brassica oleracea var. botrytis L.) cv. Pusa Ashwani”. The experiment was laid out in randomized block design with three replications and eleven treatments. The results showed that plants treated with integrated application of inorganic fertilizer, Vermicompost, Azotobacter, PSB (Phosphate Solubilizing Bacteria), Black mulch (2.5 mm) proved significantly better over the recommended practice 100% RDF (Recommended doses of fertilizer) and control. However, plants treated without organic manures and inorganic fertilizers resulted in significant reduction for various growth, yield and quality parameters. Among the treatments, plants fortified with application of 50% RDF + 15 t/ha Vermicompost + Azotobacter (5 kg/ha) + PSB (5kg/ha) + Black mulch (2.5 mm) was found to better growth, yield and quality for both years.

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Influence of Bio-fertilizers and Botanicals on GrowthYield and Yield Attributes of Field Pea (Pisum sativum L.) Var. (Azad Pea-3)

International Journal of Environment and Climate Change ◽

10.9734/ijecc/2021/v11i1130525 ◽

2021 ◽

pp. 142-147

Author(s):

P. Navya Sai Mrudula ◽

Abhinav Dayal ◽

Prashant Kumar Rai ◽

G. M. Lal ◽

Neha Thomas

Keyword(s):

Leaf Extract ◽

Block Design ◽

Growth Yield ◽

Phosphate Solubilizing Bacteria ◽

Yield Attributes ◽

Randomized Block Design ◽

Emergence Percentage ◽

Field Experimentation ◽

Phosphate Solubilizing ◽

Moringa Leaf Extract

The Field Experiment was conducted at Field Experimentation Centre, Department of Genetics and Plant Breeding, Sam Higginbottom University of Agricultural Sciences and Technology, Prayagraj (U.P) during the year 2020-2021.The Experiment was laid out in a Randomized Block Design with 13 treatments and 3 replications. The treatments were T0 - Control, T1- Azotobacter @ 3g, T2-Azotobacter @ 5g ,T3- Phosphate Solubilizing Bacteria @ 3g, T4-Phosphate Solubilizing Bacteria @ 5g, T5- Azospirillum @ 3g, T6-Azospirillum@ 5g,T7- Rhizobium @ 3g, T8-Rhizobium @ 5g , T9- Moringa leaf extract @1%, T10-Moringa leaf extract @3%, T11- Lantana camera leaf extract @1%, T12-Lantana camera leaf extract @ 3% with a soaking duration of 8 hours. The results showed that seeds primed with T8- Rhizobium @ 5g improved Field emergence percentage, growth, yield and yield attributing traits followed by T7-Rhizobium @ 3g and T4- PSB@ 5g. The least performance was observed in T0-Control when compared with the other treatments. This it indicated that the process of seed treatment by bio-fertilizers may be the better option for seed growers to achieve higher seed yield and yield attributes in Pea.

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