scholarly journals Frequency of toxocara infection in children attended by the health public service of Maringá, south Brazil

2007 ◽  
Vol 49 (6) ◽  
pp. 343-348 ◽  
Author(s):  
Márcia L. Paludo ◽  
Dina L.M. Falavigna ◽  
Guita R. Elefant ◽  
Mônica L. Gomes ◽  
Magda L.M. Baggio ◽  
...  
Keyword(s):  
Public Service ◽  
Signs And Symptoms ◽  
Toxocara Canis ◽  
Public Health Services ◽  
Serum Samples ◽  
Positive Serology ◽  
Gastrointestinal Problems ◽  
Prevention Activities ◽  
Secretory Products ◽  
South Brazil

The lack of specific laboratorial diagnosis methods and precise symptoms makes the toxocariasis a neglected disease in Public Health Services. This study aims to determine the frequency of Toxocara spp. infection in children attended by the Health Public Service of Hospital Municipal de Maringá, South Brazil. To evaluate the association of epidemiological and clinical data, an observational and cross-section study was carried out. From 14,690 attended children/year aged from seven month to 12 years old, 450 serum samples were randomly collected from September/2004 to September/2005. A questionnaire was used to evaluate epidemiological, clinical and hematological data. An ELISA using Toxocara canis larval excretory-secretory products as antigen detected 130 (28.8%) positive sera, mainly between children from seven month to five years old (p = 0.0016). Significant correlation was observed between positive serology for Toxocara, and frequent playing in sandbox at school or daycare center (p = 0.011) and the presence of a cat at home (p = 0.056). From the families, 50% were dog owners which exposed soil backyards. Eosinophilia (p = 0.776), and signs and symptoms analyzed (fever p = 0.992, pneumonia p = 0.289, cold-like symptoms p = 0.277, cough p = 0.783, gastrointestinal problems p = 0.877, migraine p = 0.979, abdominal pain p = 0.965, joint pain p = 0.686 and skin rash p = 0.105) could not be related to the presence of anti-Toxocara antibodies. Therefore, two asthmatics children showed titles of 1:10,240 and accentuated eosinophilia (p = 0.0001). The authors emphasize the needs of prevention activities.

scholarly journals Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 725
Author(s):  
David Becerro-Recio ◽  
Javier González-Miguel ◽  
Alberto Ucero ◽  
Javier Sotillo ◽  
Álvaro Martínez-Moreno ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Fasciola Hepatica ◽  
Cathepsin L ◽  
Helminth Parasites ◽  
Glutathione S Transferase ◽  
Serum Samples ◽  
Secretory Products ◽  
Immunogenic Proteins ◽  
Host Parasite ◽  
First Time

Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.

scholarly journals Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

1992 ◽  
Vol 34 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Eide Dias Camargo ◽  
Paulo Mutuko Nakamura ◽  
Adelaide José Vaz ◽  
Marcos Vinícius da Silva ◽  
Pedro Paulo Chieffi ◽  
...  
Keyword(s):  
Low Cost ◽  
Clinical Signs ◽  
Toxocara Canis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Normal Individuals ◽  
Before And After ◽  
Dot Elisa ◽  
Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 351-357 ◽  
Author(s):  
H. D. F. H. Schallig ◽  
M. A. W. van Leeuwen ◽  
W. M. L. Hendrikx
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
The Other ◽  
Proliferation Index ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Molecular Weights ◽  
Secretory Products

SUMMARYThe excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

scholarly journals Espondilodiscite Brucélica: Casuística dos Últimos 25 Anos

2014 ◽  
Vol 27 (2) ◽  
pp. 204 ◽  
Author(s):  
Ana Lebre ◽  
Jorge Velez ◽  
Diana Seixas ◽  
Eduardo Rabadão ◽  
Joaquim Oliveira ◽  
...  
Keyword(s):  
Average Duration ◽  
Signs And Symptoms ◽  
Neurological Deficits ◽  
Imaging Method ◽  
Low Back ◽  
Positive Serology ◽  
Abscess Drainage ◽  
Duration Of Symptoms ◽  
Spinal Magnetic Resonance Imaging ◽  
Appropriate Therapy

<strong>Introduction:</strong> Brucellosis is an endemic zoonosis in Portugal. Brucellar spondylodiscitis is one of the most frequent focal manifestations which may cause severe sequelae despite appropriate therapy.<br /><strong>Material and Methods:</strong> Retrospective study of patients with diagnosis of brucellar spondylodiscitis admitted to the Infectious Diseases Department of Centro Hospitalar e Universitário de Coimbra, over a 25-year period (1988-2012).<br /><strong>Results:</strong> We identified 54 patients, 55.6% male, mean age of 54.8 years. In 81.5% an epidemiological context was identified, mostly contact with sheep and goats. The duration of symptoms prior to diagnosis was 5.5 months. The most common signs and symptoms were pain (98.1%), fever (46.3%) and neurological deficits (25.9%). Spinal magnetic resonance imaging was the most used imaging method (77.8%) showing abscesses in 29.6% of patients. Lumbar location predominated (77.7%). Diagnosis was attained in 47 patients (87.0%): positive blood cultures (3 patients), positive serology (32 patients) or by both methods (12 patients). Combined regimens of doxycycline and rifampicin (64.8%), or streptomycin (24.1%) were most used, for an average duration of 4.4 months. A patient was referred for surgery for abscess drainage. Evolution was mostly favorable (92.6%), no deaths occurring.<br /><strong>Discussion:</strong> Research of the epidemiologic context turned out to be a major key leading to the diagnosis. Treatment of osteoarticular brucellosis is still controversial.<br /><strong>Conclusions:</strong> Brucellar spondylodiscitis should be considered in the differential diagnosis of patients with low back pain, even in the absence of fever, particularly in regions where the disease is endemic. Antibiotic regimen, its’ duration and the need for surgery should be individualized to achieve a better prognosis. Cases have declined over the years, a fact related to better control of animal endemic.

scholarly journals A ten-year follow-up of human leptospirosis in Uruguay: an unresolved health problem

2012 ◽  
Vol 54 (2) ◽  
pp. 69-76 ◽  
Author(s):  
Felipe Schelotto ◽  
Elba Hernández ◽  
Sabina González ◽  
Alicia Del Monte ◽  
Silvana Ifran ◽  
...  
Keyword(s):  
Young Male ◽  
Standard Technique ◽  
Signs And Symptoms ◽  
Complete Recovery ◽  
Laboratory Diagnosis ◽  
Diagnostic Techniques ◽  
Clinical Feature ◽  
Serum Samples ◽  
Microbiological Methods ◽  
Major Factors

Leptospira spp. are delicate bacteria that cannot be studied by usual microbiological methods. They cause leptospirosis, a zoonotic disease transmitted to humans through infected urine of wild or domestic animals. We studied the incidence of this disease in the Uruguayan population, its epidemiologic and clinical features, and compared diagnostic techniques. After examining 6,778 suspect cases, we estimated that about 15 infections/100,000 inhabitants occurred yearly, affecting mainly young male rural workers. Awareness about leptospirosis has grown among health professionals, and its lethality has consequently decreased. Bovine infections were probably the principal source of human disease. Rainfall volumes and floods were major factors of varying incidence. Most patients had fever, asthenia, myalgias or cephalalgia, with at least one additional abnormal clinical feature. 30-40% of confirmed cases presented abdominal signs and symptoms, conjunctival suffusion and altered renal or urinary function. Jaundice was more frequent in patients aged > 40 years. Clinical infections followed an acute pattern and their usual outcome was complete recovery. Laboratory diagnosis was based on indirect micro-agglutination standard technique (MAT). Second serum samples were difficult to obtain, often impairing completion of diagnosis. Immunofluorescence was useful as a screening test and for early detection of probable infections.

scholarly journals Successful capture of Toxocara canis larva antigens from human serum samples

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Aarón Rodríguez-Caballero ◽  
Mario Noé Martínez-Gordillo ◽  
Yolanda Medina-Flores ◽  
María Edith Medina-Escutia ◽  
Antonio Meza-Lucas ◽  
...  
Keyword(s):  
Human Serum ◽  
Toxocara Canis ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Successful Capture

Nexus of E-government, cybersecurity and corruption on public service (PSS) sustainability in Asian economies using fixed-effect and random forest algorithm

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Hafiz Syed Mohsin Abbas ◽  
Zahid Hussain Qaisar ◽  
Xiaodong Xu ◽  
Chunxia Sun
Keyword(s):  
Random Forest ◽  
Public Service ◽  
Fixed Effect ◽  
Control Measures ◽  
Algorithm Analysis ◽  
Public Health Services ◽  
Content Type ◽  
Computational Estimation ◽  
Asian Economies ◽  
Independent Variable

PurposeE-government development (EGD) is vital in enhancing the institutional quality and sustainable public service (SPS) delivery by eradicating corruption and cybersecurity crimes.Design/methodology/approachThe present study applied econometric fixed-effect (FE) regression analysis and random forest (RF) algorithm through machine learning for comprehensive estimations in achieving SPS. This study gauges the nexus between the EGD as an independent variable and public service sustainability (PSS) as a proxy of public health services as a dependent variable in the presence of two moderators, corruption and cybersecurity indices from 47 Asian countries economies from 2015 to 2019.FindingsThe computational estimation and econometric findings show that EGD quality has improved with time in Asia and substantially promoted PSS. It further explores that exercising corruption control measures and introducing sound cybersecurity initiatives enhance PSS's quality and support the EDG effect much better.Practical implicationsThe study concludes that E-Government has positively impacted PSS (healthcare) in Asia while controlling cybersecurity and institutional malfunctioning made an E-Government system healthier and SPS development in Asia.Originality/valueThis study added a novel contribution to existing E-Government and public services literature by comprehensively applied FE regression and RF algorithm analysis. Moreover, E-Government and cybersecurity improvement also has taken under consideration for PSS in Asian economies.

scholarly journals Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Quang Huy Vu ◽  
Diep Tuan Tran ◽  
Phu Manh Sieu Tran ◽  
Van Chuong Le ◽  
Thi Diem Phuc Huynh ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Western Blotting ◽  
Freeze Drying ◽  
External Quality Assessment ◽  
Toxocara Canis ◽  
Serum Samples ◽  
External Quality ◽  
Experimental Procedure ◽  
The Stability ◽  
Anti Hiv

Background. External quality assessment (EQA) provides evidence of reliable, accurate, and precise results for customers using the diagnostic test for Toxocara canis. Objective. To establish a procedure for producing standard Toxocara canis serum samples for serodiagnostic testing in EQA. Methods. The collected serum samples to contain anti-Toxocara canis antibodies were screened by ELISA and confirmed by Western blotting. These samples were found to be negative for other helminth antibodies, anti-HIV-1 and -2 antibodies, anti-HCV antibodies, and antibodies to HBs antigen. The sera were divided, processed by both freeze-drying and freezing methods, and then stored. The stability and homogeneity of the samples were evaluated after 7 days, 1 month, 3 months, and 6 months. An F-test and a T-test were applied to evaluate their homogeneity and stability. Results. Among eleven samples positive by ELISA, ten of them were confirmed via Western blotting by positive reaction with 5 specific Toxocara canis bands. Two lots of trial standard sera containing specific anti-Toxocara canis antibodies were successfully produced. Lot DK had a concentration of 31.01±1.1 NovaTec Units (NTU), and Lot DL had a concentration of 27.18±0.9 NTU. After storage at -80°C, the samples prepared by the freeze-drying method were stable for at least 3 months, and the samples prepared by the freezing method were stable for 6 months (p>0.05). Samples produced by both methods were stable for 7 days at 30°C (p>0.05). Conclusion. Specific serodiagnosis samples of anti-Toxocara canis antibodies for EQA could be produced that possessed homogeneity and stability lasting for 3 months and 6 months by the freeze-drying and freezing methods, respectively. At 30°C, the samples produced by both methods were stable for 7 days, suitable for delivery to remote laboratories.

scholarly journals The somatic proteins of Toxocara canis larvae and excretory-secretory products revealed by proteomics

2018 ◽  
Vol 259 ◽  
pp. 25-34 ◽  
Author(s):  
Márcia B. da Silva ◽  
Juan R. Urrego A. ◽  
Yisela Oviedo ◽  
Philip J. Cooper ◽  
Luis G.C. Pacheco ◽  
...  
Keyword(s):  
Toxocara Canis ◽  
Secretory Products

Gastrointestinal emergencies

2016 ◽  
Keyword(s):  
Abdominal Pain ◽  
Gastrointestinal Tract ◽  
Gastrointestinal Symptoms ◽  
Disease Process ◽  
Signs And Symptoms ◽  
Urgent Care ◽  
Nursing Assessment ◽  
Initial Management ◽  
Abdominal Organs ◽  
Gastrointestinal Problems

Problems relating to the gastrointestinal system are frequently seen in emergency and urgent care settings. Gastrointestinal signs and symptoms may relate directing to a problem in the gastrointestinal tract or may be a feature of another disease process. For example, vomiting and abdominal pain are often seen in diabetic ketoacidosis. This chapter provides detailed guidance on how to assess a patient with abdominal pain and other gastrointestinal symptoms. Appropriate investigations are identified, with their suggested indications. The remainder of this chapter covers the nursing assessment, investigations, and initial management of a comprehensive list of gastrointestinal problems, including injuries to abdominal organs.

Sign in / Sign up

Export Citation Format

Share Document