scholarly journals NATURAL INFECTION BY Trypanosoma cruzi IN ONE DOG IN CENTRAL WESTERN BRAZIL: A CASE REPORT

2013 ◽  
Vol 55 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Arleana do Bom Parto Ferreira de Almeida ◽  
Daphine Ariadne Jesus de Paula ◽  
Maria Luisa Paro Otton ◽  
Felipe Wolf Jaune ◽  
Raquel Aparecida Sales da Cruz ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Natural Infection ◽  
Human Populations ◽  
Direct Examination ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Blood Smears ◽  
Polymerase Chain ◽  
Acute Chagas Disease

SUMMARY It is estimated that about 10 million people are infected with Trypanosoma cruzi worldwide, mostly in Latin America and more than 25 million are at risk of acquiring this infection in endemic areas. Dogs are an important reservoir for this pathogen and thus, considered a risk factor for human populations. This report describes one case of Chagas disease in a dog from Cuiabá, Mato Grosso State, Brazil. The diagnosis was obtained by direct examination of trypomastigote forms in blood smears. Amastigotes forms were visualized in microscopy of the bone marrow, lymph nodes, kidneys, liver and brain. The T. cruzi (ZIII) infection was confirmed by Polymerase Chain Reaction, and sequencing. The animal presented multisystemic failure and died. Although acute Chagas disease in humans is not reported in Cuiabá, this is the first report of a canine case in this region. This case represents a warning, to health professionals and authorities, to the possibility of transmission of this zoonosis in Cuiabá.

scholarly journals Natural infection by Trypanosoma cruzi in dogs located in Ituberá, Southern Bahia, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 881 ◽  
Author(s):  
Graziela Baroni de Souza ◽  
Paula Elisa Brandão Guedes ◽  
Thaís Nascimento De Andrade Oliveira ◽  
Fábio Santos Carvalho ◽  
George Rêgo Albuquerque ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Natural Infection ◽  
Local Health ◽  
Chain Reaction ◽  
Health Authorities ◽  
Southern Bahia ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Local Health Authorities ◽  
The City

Chagas disease, caused by the flagellate protozoan Trypanosoma cruzi, is an anthropozoonosis of great importance for public health. Several species of mammals are reservoirs for this parasite, including dogs. The objective of this work was to verify the natural infection by T. cruzi in the population of dogs of the municipality of Ituberá, Bahia. A total of 392 domiciled dogs from all districts of the city were evaluated; five milliliters of blood was collected from the dogs for molecular diagnosis. T. cruzi DNA was amplified through the polymerase chain reaction (PCR); the primers P35 and P36, which amplify a fragment of 330 bp, were used. The PCR products were subjected to 2% agarose gel electrophoresis containing Sybr (Invitrogen). Of the 392 dogs evaluated, only 2 (0.51%) animals, one male and one female, tested positive for T. cruzi. This study concluded that there are dogs naturally infected by T. cruzi in the municipality of Ituberá-Bahia and that this finding is an alert to veterinarians, health professionals, and local health authorities that their own dogs can act as reservoirs of the disease.

scholarly journals Severe digestive pathology associated with chronic Chaga's disease in Ecuador: report of two cases

1997 ◽  
Vol 30 (5) ◽  
pp. 389-392 ◽  
Author(s):  
Angel G. Guevara ◽  
José W. Eras ◽  
Marcelo Recalde ◽  
Luis Vinueza ◽  
Philip J. Cooper ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Peripheral Blood ◽  
Secretory Protein ◽  
Clinical Findings ◽  
Primary Sequence ◽  
Chain Reaction ◽  
Andean Countries ◽  
Polymerase Chain

DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.

scholarly journals HIV and Chagas Disease: An Evaluation of the Use of Real-Time Quantitative Polymerase Chain Reaction to Measure Levels of Trypanosoma cruzi Parasitemia in HIV Patients in Cochabamba, Bolivia

2021 ◽  
Author(s):  
Melissa J. Reimer-McAtee ◽  
Carolina Mejia ◽  
Taryn Clark ◽  
Jules Terle ◽  
Monica J. Pajuelo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Real Time ◽  
Chagas Disease ◽  
Blood Clot ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Positive Serology ◽  
Polymerase Chain ◽  
Chronic Chagas Disease

This cross-sectional study evaluated epidemiologic characteristics of persons living with HIV (PWH) coinfected with Trypanosoma cruzi in Cochabamba, Bolivia, and estimated T. cruzi parasitemia by real-time quantitative polymerase chain reaction (qPCR) in patients with and without evidence of reactivation by direct microscopy. Thirty-two of the 116 HIV patients evaluated had positive serology for T. cruzi indicative of chronic Chagas disease (27.6%). Sixteen of the 32 (50%) patients with positive serology were positive by quantitative polymerase chain reaction (qPCR), and four of the 32 (12.5%) were positive by direct microscopy. The median parasite load by qPCR in those with CD4+ < 200 was 168 parasites/mL (73-9951) compared with 28.5 parasites/mL (15–1,528) in those with CD4+ ≥ 200 (P = 0.89). There was a significant inverse relationship between the degree of parasitemia estimated by qPCR from blood clot and CD4+ count on the logarithmic scale (rsBC= –0.70, P = 0.007). The correlation between T. cruzi estimated by qPCR+ blood clot and HIV viral load was statistically significant with rsBC = 0.61, P = 0.047. Given the significant mortality of PWH and Chagas reactivation and that 57% of our patients with CD4+ counts < 200 cells/mm3 showed evidence of reactivation, we propose that screening for chronic Chagas disease be considered in PWH in regions endemic for Chagas disease and in the immigrant populations in nonendemic regions. Additionally, our study showed that PWH with advancing immunosuppression have higher levels of estimated parasitemia measured by qPCR and suggests a role for active surveillance for Chagas reactivation with consideration of treatment with antitrypanosomal therapy until immune reconstitution can be achieved.

scholarly journals Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Martha Alicia Ballinas-Verdugo ◽  
Rogelio Frank Jiménez-Ortega ◽  
Eduardo Martínez-Martínez ◽  
Nancy Rivas ◽  
Erick Abraham Contreras-López ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Extracellular Vesicles ◽  
Bioinformatics Analysis ◽  
Chronic Phase ◽  
Heart Tissue ◽  
Detailed Knowledge ◽  
Total Plasma ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Relevant Finding

Abstract Background Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. MicroRNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-β signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction. Results Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated. Conclusions The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.

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