scholarly journals Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Martha Alicia Ballinas-Verdugo ◽  
Rogelio Frank Jiménez-Ortega ◽  
Eduardo Martínez-Martínez ◽  
Nancy Rivas ◽  
Erick Abraham Contreras-López ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Extracellular Vesicles ◽  
Bioinformatics Analysis ◽  
Chronic Phase ◽  
Heart Tissue ◽  
Detailed Knowledge ◽  
Total Plasma ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Relevant Finding

Abstract Background Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. MicroRNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-β signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction. Results Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated. Conclusions The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.

scholarly journals Early polymerase chain reaction detection of Chagas disease reactivation in heart transplant patients

2017 ◽  
Vol 36 (7) ◽  
pp. 797-805 ◽  
Author(s):  
Priscilla Almeida da Costa ◽  
Marcela Segatto ◽  
Danielle Fernandes Durso ◽  
Wagson José de Carvalho Moreira ◽  
Lucas Lodi Junqueira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chagas Disease ◽  
Heart Transplant ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Transplant Patients ◽  
Disease Reactivation ◽  
Polymerase Chain

scholarly journals Detection of two alternative bcr/abl mRNA junctions and minimal residual disease in Philadelphia chromosome positive chronic myelogenous leukemia by polymerase chain reaction

Blood ◽  
1989 ◽  
Vol 73 (8) ◽  
pp. 2165-2170
Author(s):  
MS Lee ◽  
A LeMaistre ◽  
HM Kantarjian ◽  
M Talpaz ◽  
EJ Freireich ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chronic Myelogenous Leukemia ◽  
Philadelphia Chromosome ◽  
Rare Event ◽  
Chronic Phase ◽  
Myelogenous Leukemia ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Minimal Residual

The Philadelphia (Ph′) chromosome in chronic myelogenous leukemia (CML) results in fusion of the bcr gene and c-abl oncogene, which transcribes into two types of chimeric bcr/abl mRNAs: the L-6 junction and the K-28 junction. By means of a highly sensitive assay, combination of reverse transcription and polymerase chain reaction (RT/PCR), we analyzed 38 blood samples obtained from 31 patients with Ph′-positive CML and two patients with Ph′-negative bcr rearranged CML. Among the 21 samples obtained in chronic phase, eight patients had the L-6 mRNA, 11 had the K-28 mRNA, and two had both the L-6 and K-28 mRNAs. Among the nine samples obtained in blast crisis, four contained the L-6 mRNA, two contained the K-28 mRNA, and three contained both the K-28 and L-6 mRNAs. This finding supports the concept of alternative splicing of bcr/abl mRNAs transcribed in Ph′-positive CML. However, it appears to be a rare event. Of the eight samples obtained from eight patients who had achieved complete cytogenetic remission and negativity for bcr region rearrangement for 6 months to 3 years after recombinant alpha interferon (r alpha-IFN) therapy, all of them showed evidence of minimal residual Ph′-positive clones as detected by the RT/PCR assay. This finding suggests that interferon therapy suppresses the proliferation of the Ph′-positive clones, but it does not completely eradicate the Ph′-positive stem cells.

scholarly journals NATURAL INFECTION BY Trypanosoma cruzi IN ONE DOG IN CENTRAL WESTERN BRAZIL: A CASE REPORT

2013 ◽  
Vol 55 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Arleana do Bom Parto Ferreira de Almeida ◽  
Daphine Ariadne Jesus de Paula ◽  
Maria Luisa Paro Otton ◽  
Felipe Wolf Jaune ◽  
Raquel Aparecida Sales da Cruz ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Natural Infection ◽  
Human Populations ◽  
Direct Examination ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Blood Smears ◽  
Polymerase Chain ◽  
Acute Chagas Disease

SUMMARY It is estimated that about 10 million people are infected with Trypanosoma cruzi worldwide, mostly in Latin America and more than 25 million are at risk of acquiring this infection in endemic areas. Dogs are an important reservoir for this pathogen and thus, considered a risk factor for human populations. This report describes one case of Chagas disease in a dog from Cuiabá, Mato Grosso State, Brazil. The diagnosis was obtained by direct examination of trypomastigote forms in blood smears. Amastigotes forms were visualized in microscopy of the bone marrow, lymph nodes, kidneys, liver and brain. The T. cruzi (ZIII) infection was confirmed by Polymerase Chain Reaction, and sequencing. The animal presented multisystemic failure and died. Although acute Chagas disease in humans is not reported in Cuiabá, this is the first report of a canine case in this region. This case represents a warning, to health professionals and authorities, to the possibility of transmission of this zoonosis in Cuiabá.

scholarly journals SPAG4L/SPAG4Lβ interacts with Nesprin2 to participate in the meiosis of spermatogenesis

2019 ◽  
Vol 51 (7) ◽  
pp. 669-676 ◽  
Author(s):  
Xiaohua Li ◽  
Yong Wu ◽  
Lihua Huang ◽  
Linfei Yang ◽  
Xiaowei Xing
Keyword(s):  
Bioinformatics Analysis ◽  
Immunofluorescence Assay ◽  
Human Testis ◽  
Interacting Proteins ◽  
Chain Reaction ◽  
Meiotic Process ◽  
Localization Analysis ◽  
Polymerase Chain ◽  
Sun Domain

Abstract SUN domain proteins are identified as a novel family of nuclear envelope proteins which are involved in spermatogenesis. SPAG4L is identified as the fifth member of this family. Previous studies have revealed that SPAG4L is involved in spermatogenesis and the mutations occurring in SPAG4L will lead to male infertility. However, the transcriptions of SPAG4L and its interacting proteins in the testis are still unclear. In this study, we identified a shorter transcript variant of SPAG4L, named SPAG4Lβ, in human testis by northern blot and reverse transcription-polymerase chain reaction. Bioinformatics analysis showed that it encodes a protein consisting of 311 amino acids, and subcellular localization analysis revealed that it is mainly expressed in the cytoplasm. In situ hybridization and immunofluorescence assay revealed that SPAG4L/SPAG4Lβ is involved in meiosis. Furthermore, co-IP results demonstrated that SPAG4L/SPAG4Lβ interacts with Nesprin2, a KASH domain protein to form the LINC (linker of nucleoskeleton and cytoskeleton) complexes. Immunofluorescence results revealed that the LINC complexes of Spag4l/Nesprin2 in mouse are involved in spermatocyte division. Our data indicated that SPAG4L/SPAG4Lβ may play an important role in the meiotic process.

scholarly journals Severe digestive pathology associated with chronic Chaga's disease in Ecuador: report of two cases

1997 ◽  
Vol 30 (5) ◽  
pp. 389-392 ◽  
Author(s):  
Angel G. Guevara ◽  
José W. Eras ◽  
Marcelo Recalde ◽  
Luis Vinueza ◽  
Philip J. Cooper ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Peripheral Blood ◽  
Secretory Protein ◽  
Clinical Findings ◽  
Primary Sequence ◽  
Chain Reaction ◽  
Andean Countries ◽  
Polymerase Chain

DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.

scholarly journals In Nonendemic Areas, Is Microscopy Better than Polymerase Chain Reaction for Diagnosis of Congenital Chagas Disease?

2010 ◽  
Vol 50 (2) ◽  
pp. 279-280 ◽  
Author(s):  
Luis Ignacio Gonzalez‐Granado ◽  
Gema Guillen‐Fiel ◽  
Pablo Rojo‐Conejo
Keyword(s):  
Polymerase Chain Reaction ◽  
Chagas Disease ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Better Than
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