scholarly journals Human parvovirus B19 infections among exanthematic diseases notified as measles

1997 ◽  
Vol 30 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Rita C.N. Cubel ◽  
Marilda M. Siqueira ◽  
Elisabete O. Santos ◽  
Maria F. Pires ◽  
Célia M. F. Cruz ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Human Parvovirus B19 ◽  
Igg Antibodies ◽  
Dot Blot ◽  
Dot Blot Hybridization

A total of 1397 sera collected from 1095 cases of exanthematic disease notified as measles in ES and RJ states during July 1992 to December 1994 were investigated. These sera were first tested for measles and rubella specific IgM. When they proved negative, they were tested for B19 specific IgM by an enzyme immunoassay. B19 infection was confirmed in 27 (2.5%) of these cases. Sera from 194 negative cases for measles and rubella IgM received from other Brazilian states were also investigated and B19 infection was confirmed for 11 of them. Sera from these 38 IgM positive cases for B19, were tested for anti-B19 IgG by an enzyme immunoassay and for B19 DNA by dot blot hybridization. Anti-B19 IgG antibodies were detected in most of the acute sera. B19 DNA was detected in the acute serum of one patient that had been splenectomized before. As the exanthem caused by human parvovirus infection may be clinically diagnosed as rubella, it could be important to diagnose B19 infection in Brazil since it is becoming prevalent as the cause of rash in countries where rubella is controlled by vaccination.

Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples

2000 ◽  
Vol 302 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
Marialuisa Zerbini ◽  
Monica Cricca ◽  
Giovanna Gentilomi ◽  
Simona Venturoli ◽  
Giorgio Gallinella ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Hybridization Assay ◽  
Tyramide Signal Amplification ◽  
Dot Blot ◽  
Serum Samples ◽  
Dot Blot Hybridization

Chemiluminescent Immunoperoxidase Assay for the Dot Blot Hybridization Detection of Parvovirus B19 DNA Using a Low Light Imaging Device

1996 ◽  
Vol 236 (2) ◽  
pp. 290-295 ◽  
Author(s):  
Stefano Girotti ◽  
Monica Musiani ◽  
Elida Ferri ◽  
Giorgio Gallinella ◽  
Marialuisa Zerbini ◽  
...  
Keyword(s):  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Low Light ◽  
Imaging Device ◽  
Dot Blot Hybridization ◽  
Immunoperoxidase Assay ◽  
Hybridization Detection

Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization

2003 ◽  
pp. 173-188
Author(s):  
Karen E. Hicks ◽  
Stuart Beard ◽  
Bernard J. Cohen ◽  
Jonathan P. Clewley
Keyword(s):  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Dot Blot Hybridization

Feasibility of Qualitative Testing of BCR-ABL and JAK2 V617F in Suspected Myeloproliperative Neoplasm (MPN) Using RT-PCR Reversed Dot Blot Hybridization (RT-PCR RDB)

2019 ◽  
Vol 19 (4) ◽  
pp. 220-227
Author(s):  
Najmiatul Masykura ◽  
Ummu Habibah ◽  
Siti Fatimah Selasih ◽  
Soegiarto Gani ◽  
Cosphiadi Irawan ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Jak2 V617f ◽  
Rt Pcr ◽  
Dot Blot ◽  
Dot Blot Hybridization

Prevalence of HPV in a Melbourne Female STD Population: Comparison of RNA and DNA Probes in Detecting HPV by Dot Blot Hybridization

1993 ◽  
Vol 4 (3) ◽  
pp. 159-164 ◽  
Author(s):  
A J Borg ◽  
G Medley ◽  
S M Garland
Keyword(s):  
Past History ◽  
Blot Hybridization ◽  
Condylomata Acuminata ◽  
Dot Blot ◽  
Hpv Dna ◽  
Sexually Transmitted ◽  
Dot Blot Hybridization ◽  
History Of ◽  
Cytological Features ◽  
Dna Types

A total of 377 women, consecutively selected as first attenders to a sexually transmitted diseases clinic in Melbourne, Australia, were examined for overt Condylomata acuminata and were screened for genital HPV DNA types 6, 11, 16, 18, 31, 33 and (35) using 2 dot blot hybridization methods. Overall, there was a 90% positivity correlation between the 2 methods with HPV DNA being detected in 12% of ectocervical samples. Overt warts were found in 15% of the women and HPV DNA was detected at the cervix in 35% with cytology predicting HPV with or without dysplasia in 27%. Thirteen percent had a past history of warts but none on examination and HPV DNA was evident in 16% while 18% had cytological features of HPV. Those with no warts evident and no past history of warts had both HPV DNA and cytological features of HPV in 7%.

Detection of herpes simplex virus and adenovirus DNA by dot blot hybridization using in vitro synthesized RNA transcripts

1986 ◽  
Vol 13 (4) ◽  
pp. 291-299 ◽  
Author(s):  
Volker Schuster ◽  
Bertfried Matz ◽  
Helga Wiegand ◽  
Brigitte Traub ◽  
Dieter Neumann-Haefelin
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Rna Transcripts ◽  
Dot Blot Hybridization ◽  
Simplex Virus

Detection of Cryptosporidium parvum Oocysts on Fresh Vegetables and Herbs Using Antibodies Specific for a Cryptosporidium parvum Viral Antigen

2005 ◽  
Vol 68 (5) ◽  
pp. 1093-1096 ◽  
Author(s):  
K. E. KNIEL ◽  
M. C. JENKINS
Keyword(s):  
Cryptosporidium Parvum ◽  
Viral Antigen ◽  
Blot Hybridization ◽  
Surface Antigens ◽  
Food Samples ◽  
Sensitive Detection ◽  
Dot Blot ◽  
Dot Blot Hybridization ◽  
Fresh Vegetables ◽  
Cryptosporidium Parvum Oocysts

The purpose of this study was to determine if the viral symbiont of Cryptosporidium parvum (CPV) sporozoites could be used as a target for sensitive detection of the parasite in food samples. Polyclonal sera specific to the recombinant viral capsid protein (rCPV40) was used in a dot blot hybridization assay to detect oocysts recovered from green onions and cilantro. Small batches of chopped green onions and cilantro leaves were artificially contaminated with three different concentrations of oocysts: 106, 102, and 101. rCPV40 was superior in detecting oocysts compared with other antibodies directed toward total oocyst protein and oocyst surface antigens. This study provides evidence that CPV is an excellent target for sensitive detection of C. parvum oocysts in foods.

Sign in / Sign up

Export Citation Format

Share Document