scholarly journals Diagnosis of hantavirus infection in humans and rodents in Ribeirão Preto, State of São Paulo, Brazil

2010 ◽  
Vol 43 (4) ◽  
pp. 348-354 ◽  
Author(s):  
Glauciane Garcia de Figueiredo ◽  
Alessandra Abel Borges ◽  
Gelse Mazzoni Campos ◽  
Alex Martins Machado ◽  
Fabiano Pinto Saggioro ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Nucleotide Sequences ◽  
Hantavirus Infection ◽  
São Paulo ◽  
Wild Rodents ◽  
Rt Pcr ◽  
School Of Medicine ◽  
Causative Agents ◽  
Serious Disease ◽  
Araraquara Virus

INTRODUCTION: Hantavirus pulmonary and cardiovascular syndrome (HPCS) is an emerging serious disease in the Americas. Hantaviruses (Bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. In the Ribeirão Preto region (state of São Paulo, Brazil), HPCS has been reported since 1998, caused by the Araraquara virus (ARAV), for which Necromys lasiurus is the rodent reservoir. This study aimed to show diagnostic results relating to infection in humans and rodents, obtained at the Virology Research Center of the Ribeirão Preto School of Medicine, University of São Paulo, between 2005 and 2008. METHODS: HPCS was diagnosed by means of ELISA and/or RT-PCR in 11 (21.2%) out of 52 suspected cases, and 54.4% of these were fatal. Furthermore, 595 wild rodents (Necromys lasiurus, Akodon sp, Calomys tener and Oligoryzomys sp) were caught between 2005 and 2008. RESULTS: Fifteen (2.5%) of these rodents presented antibodies for hantavirus, as follows: Necromys lasiurus (4%), Calomys tener (1.9%) and Akodon sp (1.5%). Nucleotide sequences obtained through RT-PCR from one HPCS patient and one Calomys tener rodent were compared with hantavirus sequences from GenBank, which showed that both were homologous with ARAV. CONCLUSIONS: This work corroborates previous studies showing that ARAV is the hantavirus causing HPCS in the Ribeirão Preto region. It also shows that rodents infected with hantavirus represent a constant risk of transmission of this virus to man.

scholarly journals Evidence of hantavirus infection in wild rodents captured in a rural area of the state of São Paulo, Brazil

2004 ◽  
Vol 24 (2) ◽  
pp. 71-73 ◽  
Author(s):  
Elba Regina S. de Lemos ◽  
Paulo S. D'Andrea ◽  
Cibele R. Bonvicino ◽  
Kátia M. Famadas ◽  
Paula Padula ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Hantavirus Infection ◽  
São Paulo ◽  
Wild Rodents ◽  
Myocastor Coypus ◽  
Suburban Areas ◽  
Andes Virus ◽  
Etiological Agents ◽  
Antigen Antibody ◽  
Oligoryzomys Flavescens

Hantaviruses are the etiological agents of the Hantavirus Cardio-Pulmonary Syndrome, a serious rodent-borne disease in Brazil. In order to investigate the occurrence of hantavirus infection in wild rodents, a survey was conducted in three different suburban areas of the municipality of Pedreira, State of São Paulo, Brazil. Of the 145 wild animals captured belonging to 12 different species identified by morphology and karyological analysis, 107 were rodents of the following species: Akodon montensis, Bolomys lasiurus, Calomys tener, Oligoryzomys nigripes, Oligoryzomys flavescens, and Myocastor coypus. Blood samples from these rodents were assayed for the presence of antibodies against hantavirus by IgG ELISA using Andes recombinant nucleocapsid antigen. Antibody reactive to Andes virus was found in two different species, O. nigripes and O. flavescens. These results indicate a potential risk for hantavirus transmission to humans in this area, where reservoir rodents are present in peridomestic settings.

scholarly journals Caracterização do Tomato chlorotic spot virus isolado de jiló no Vale do Paraíba, Estado de São Paulo

2002 ◽  
Vol 27 (3) ◽  
pp. 285-291 ◽  
Author(s):  
MARCELO EIRAS ◽  
ALEXANDRE L. R. CHAVES ◽  
ADDOLORATA COLARICCIO ◽  
RICARDO HARAKAVA ◽  
JANSEN DE ARAUJO ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Sao Paulo ◽  
Ringspot Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Os tospovírus são responsáveis por perdas significativas em diversas culturas, principalmente solanáceas. No município de São José dos Campos (SP), plantas de jiló (Solanum gilo) apresentando sintomas de mosaico, bolhosidades, nanismo e queda acentuada da produção foram coletadas para análise. Visando a caracterização do agente causador dos sintomas, testes biológicos, elétrono microscópicos, sorológicos e moleculares foram realizados. Através de inoculação mecânica em plantas indicadoras das famílias Amaranthaceae, Chenopodiaceae e Solanaceae obtiveram-se resultados típicos aos esperados para tospovírus. Ao microscópio eletrônico de transmissão, observaram-se, em contrastação negativa, partículas pleomórficas com diâmetro entre 80 e 110 nm e em cortes ultra-finos partículas presentes em vesículas do retículo endoplasmático. Através de DAS-ELISA, identificou-se o Tomato chlorotic spot virus (TCSV). A partir de RNA total extraído de folhas infetadas, amplificaram-se, via RT-PCR, fragmentos correspondentes ao gene da proteína do capsídeo (cp) os quais foram seqüenciados e comparados com outros depositados no "GenBank". A homologia de nucleotídeos e aminoácidos deduzidos foi respectivamente de 99 e 95% quando comparada com seqüências de isolados de TCSV. A comparação com as outras espécies do gênero Tospovirus apresentou valores de homologia entre 72 e 84%. Estes resultados confirmam a identidade deste vírus como pertencente à espécie TCSV, que é predominante no Estado de São Paulo e importante patógeno de outras plantas cultivadas. Além disso, variedades de jiló quando inoculadas foram susceptíveis tanto ao TCSV como às espécies Tomato spotted wilt virus (TSWV) e Groundnut ringspot virus (GRSV).

scholarly journals Isolado do vírus do mosaico do pepino obtido de bananeira no Estado de São Paulo pertence ao subgrupo Ia

2001 ◽  
Vol 26 (1) ◽  
pp. 53-59 ◽  
Author(s):  
MARCELO EIRAS ◽  
ADDOLORATA COLARICCIO ◽  
ALEXANDRE L.R. CHAVES
Keyword(s):  
Mosaic Virus ◽  
Vigna Unguiculata ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Nicotiana Glutinosa ◽  
Musa Sp ◽  
De Se ◽  
Pcr Rflp ◽  
Das Elisa

Em 1996, foi feita a caracterização parcial de um isolado do vírus do mosaico do pepino (Cucumis mosaic virus, CMV) obtido de bananeira (Musa sp.) proveniente do município de Miracatu, SP. Com o objetivo de se determinar o subgrupo do isolado de CMV, recorreu-se às técnicas de ELISA, RT-PCR, RFLP e seqüenciamento de fragmentos de RNA genômico. Amostras de folhas infetadas, desidratadas com cloreto de cálcio e armazenadas à -20 °C desde 1994 na viroteca do Laboratório de Fitovirologia e Fisiopatologia, foram inoculadas em plantas de Nicotiana glutinosa. Dez dias após a inoculação, folhas apresentando mosaico foram utilizadas para DAS-ELISA e extração de RNAs totais. Em ELISA, houve reação apenas contra o anti-soro específico para CMV subgrupo I. Através de RT-PCR com primers desenhados para anelar em regiões conservadas da porção terminal 3' do gene da capa protéica, foi amplificado um fragmento de DNA com 486 pares de bases. O produto obtido via RT-PCR foi submetido à digestão com as enzimas EcoRI, HindIII, BamHI e MspI, obtendo-se um padrão de restrição esperado para o subgrupo I. Estes resultados foram confirmados através do seqüenciamento do produto de PCR, o qual apresentou homologia de 96% a 98% com os isolados do CMV pertencentes ao subgrupo I. Pelos sintomas observados na hospedeira diferencial Vigna unguiculata, o isolado foi confirmado como sendo do subgrupo Ia.

scholarly journals Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil

2011 ◽  
Vol 46 (4) ◽  
pp. 362-369 ◽  
Author(s):  
Marcos Cesar Gonçalves ◽  
Diogo Manzano Galdeano ◽  
Ivan de Godoy Maia ◽  
César Martins Chagas
Keyword(s):  
Mosaic Virus ◽  
Sao Paulo ◽  
Sugarcane Mosaic Virus ◽  
Maize Dwarf Mosaic Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Das Elisa

O objetivo deste trabalho foi caracterizar biológica e molecularmente três isolados de Sugarcane mosaic virus (SCMV) de lavouras de milho, analisá-los filogeneticamente e discriminar polimorfismos do genoma. Plantas com sintomas de mosaico e nanismo foram coletadas em lavouras de milho, no Estado de São Paulo e no Município de Rio Verde, GO, e seus extratos foliares foram inoculados em plantas indicadoras e submetidos à análise sorológica com antissoros contra o SCMV, contra o Maize dwarf mosaic virus (MDMV) e contra o Johnsongrass mosaic virus (JGMV). Mudas de sorgo 'Rio' e 'TX 2786' apresentaram sintomas de mosaico após a inoculação dos três isolados, e o DAS-ELISA confirmou a infecção pelo SCMV. O RNA total foi extraído e usado para amplificação por transcriptase reversa seguida de reação em cadeia de polimerase (RT-PCR). Fragmentos específicos foram amplificados, submetidos à análise por polimorfismo de comprimento de fragmento de restrição (RFLP) e sequenciados. Foi possível discriminar os genótipos de SCMV isolados de milho de outros isolados brasileiros do vírus. Alinhamentos múltiplos e análises dos perfis filogenéticos corroboram esses dados e mostram diversidade nas sequências de nucleotídeos que codificam para a proteína capsidial, o que explica o agrupamento separado desses isolados e sugere sua classificação como estirpes distintas, em lugar de simples isolados geográficos.

scholarly journals Hidra de duas cabeças: Configuração ricoeuriana e narrador impuro numa narrativa do HC-FMUSP / Two Heads Hidra: Ricoeurian Configuration and Impure Narrator in a Narrative of HC - FMUSP

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Fabiana Carelli ◽  
Andrea Funchal Lens ◽  
Amanda Cabral Carvalho Alcântara De Oliveira ◽  
Ariadne Catarine Dos Santos ◽  
Mariluz Dos Reis ◽  
...  
Keyword(s):  
Literary Theory ◽  
Paul Ricoeur ◽  
Sao Paulo ◽  
Point Of View ◽  
São Paulo ◽  
Cultural Aspects ◽  
School Of Medicine ◽  
Paul Ricœur ◽  
The University ◽  
De Medicina

ABSTRACTFrom the point of view of literary theory and comparative literature, this article aims to analyze how it is configured the narrative of life of a patient of the General and Didactic Clinic of the University of São Paulo School of Medicine, in the context of a consultation with the resident who attends her, and how that narrative is reconfigured by the same resident, both in the conversation with her assistant and at the resumption of the consultation with the patient, in which diagnostic hypotheses, predictions and treatments will be transmitted. The analysis undertaken here is based mainly on the concepts of prefiguration, configuration and refiguration established by Paul Ricoeur in his book Time and Narrative (2010); narrator and narrative point of view, as in Arrigucci Jr. (1998) and Friedman (2002); and the cultural aspects of the comic genre, as in Aristotle (s/d), Darnton (1996), Bakhtin (1999) and Baudelaire (2002). In conclusion, this paper aims to propose some analytical and theoretical grounds for the concept of a “cleaved’ or “impure” narrator in the context of the relations between narrative and medicine.RESUMENEste artigo busca analisar, do ponto de vista da teoria literária e da literatura comparada, o modo como é configurada, por ela mesma, a narrativa de vida de uma paciente do Ambulatório Geral e Didático do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo num contexto de consulta com a médica residente que a atende, e de que modo essa narrativa é reconfigurada pela mesma residente, tanto na conversa com seu assistente quanto na retomada da consulta com a paciente, na qual hipóteses diagnósticas, prognósticos e tratamento lhe serão transmitidos. A análise empreendida aqui funda-se essencialmente nos conceitos de prefiguração, configuração e refiguração, tal como estabelecidos por Paul Ricoeur em sua obra Tempo e narrativa (2010); narrador e ponto de vista narrativo, tal como em Arrigucci Jr. (1998) e Friedman (2002); e do riso em suas articulações culturais, tal como em Aristóteles (s/d), Darnton (1996), Bakhtin (1999) e Baudelaire (2002). Ao final, este trabalho visa a propor bases analíticas e teóricas para a definição do conceito de narrador “clivado” ou “impuro”, no contexto das relações entre narrativa e medicina.

scholarly journals St. Louis encephalitis vírus: first isolation from a human in São Paulo state, Brasil

2005 ◽  
Vol 47 (5) ◽  
pp. 281-285 ◽  
Author(s):  
Iray M. Rocco ◽  
Cecília L.S. Santos ◽  
Ivani Bisordi ◽  
Selma M.C.N. Petrella ◽  
Luiz E. Pereira ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Case ◽  
Sao Paulo ◽  
Encephalitis Virus ◽  
Cell Culture Supernatant ◽  
São Paulo ◽  
Rt Pcr ◽  
Igg Antibody ◽  
Paulo State ◽  
São Paulo State

This paper reports the isolation of St. Louis encephalitis virus (SLEV) from a febrile human case suspected to be dengue, in São Pedro, São Paulo State. A MAC-ELISA done on the patient's acute and convalescent sera was inconclusive and hemagglutination inhibition test detected IgG antibody for flaviviruses. An indirect immunofluorescent assay done on the C6/36 cell culture inoculated with the acute serum was positive for flaviviruses but negative when tested with dengue monoclonal antibodies. RNA extracted from the infected cell culture supernatant was amplified by RT-PCR in the presence of NS5 universal flavivirus primers and directly sequenced. Results of BLAST search indicated that this sequence shares 93% nucleotide similarity with the sequence of SLEV (strain-MSI.7), confirmed by RT-PCR performed with SLEV specific primers. Since SLEV was identified as the cause of human disease, it is necessary to improve surveillance in order to achieve early detection of this agent in the state of São Paulo and in Brazil. This finding is also an alert to health professionals about the need for more complete clinical and epidemiological investigations of febrile illnesses as in the reported case. SLEV infections can be unrecognized or confused with other ones caused by an arbovirus, such as dengue.

Detection of Culex flavivirus and Aedes flavivirus nucleotide sequences in mosquitoes from parks in the city of São Paulo, Brazil

Acta Tropica ◽  
2016 ◽  
Vol 157 ◽  
pp. 73-83 ◽  
Author(s):  
Licia Natal Fernandes ◽  
Marcia Bicudo de Paula ◽  
Alessandra Bergamo Araújo ◽  
Elisabeth Fernandes Bertoletti Gonçalves ◽  
Camila Malta Romano ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Nucleotide Sequences ◽  
São Paulo ◽  
Culex Flavivirus ◽  
The City ◽  
Aedes Flavivirus

scholarly journals Reemergence of yellow fever: detection of transmission in the State of São Paulo, Brazil, 2008

2011 ◽  
Vol 44 (3) ◽  
pp. 290-296 ◽  
Author(s):  
Eduardo Stramandinoli Moreno ◽  
Iray Maria Rocco ◽  
Eduardo Sterlino Bergo ◽  
Roosecelis Araujo Brasil ◽  
Melissa Mascheratti Siciliano ◽  
...  
Keyword(s):  
Cell Culture ◽  
Yellow Fever ◽  
Virus Isolation ◽  
Yellow Fever Virus ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Epidemiological Evaluation ◽  
Entomological Research ◽  
Virus Strains

INTRODUCTION: Following yellow fever virus (YFV) isolation in monkeys from the São José do Rio Preto region and two fatal human autochthonous cases from the Ribeirão Preto region, State of São Paulo, Brazil, two expeditions for entomological research and eco-epidemiological evaluation were conducted. METHODS: A total of 577 samples from humans, 108 from monkeys and 3,049 mosquitoes were analyzed by one or more methods: virus isolation, ELISA-IgM, RT-PCR, histopathology and immunohistochemical. RESULTS: Of the 577 human samples, 531 were tested by ELISA-IgM, with 3 positives, and 235 were inoculated into mice and 199 in cell culture, resulting in one virus isolation. One sample was positive by histopathology and immunohistochemical. Using RT-PCR, 25 samples were processed with 4 positive reactions. A total of 108 specimens of monkeys were examined, 108 were inoculated into mice and 45 in cell culture. Four virus strains were isolated from Alouattacaraya. A total of 931 mosquitoes were captured in Sao Jose do Rio Preto and 2,118 in Ribeirão Preto and separated into batches. A single isolation of YFV was derived from a batch of 9 mosquitoes Psorophoraferox, collected in Urupês, Ribeirão Preto region. A serological survey was conducted with 128 samples from the municipalities of São Carlos, Rincão and Ribeirão Preto and 10 samples from contacts of patients from Ribeirão Preto. All samples were negative by ELISA-IgM for YFV. CONCLUSIONS: The results confirm the circulation of yellow fever, even though sporadic, in the Sao Paulo State and reinforce the importance of vaccination against yellow fever in areas considered at risk.

scholarly journals Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis

2008 ◽  
Vol 50 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Terezinha Lisieux Moraes Coimbra ◽  
Raimundo N. Santos ◽  
Selma Petrella ◽  
Teresa Keico Nagasse-Sugahara ◽  
Silvana Beres Castrignano ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Manifestations ◽  
Cross Reactivity ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Paulo State ◽  
São Paulo State

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

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