scholarly journals Viability of liquid medium-inoculation of Rhizobium etli in planting furrows with common bean

2018 ◽  
Vol 53 (3) ◽  
pp. 394-398
Author(s):  
Dâmiany Pádua Oliveira ◽  
Bruno Lima Soares ◽  
Fábio Aurélio Dias Martins ◽  
Leandro Alex Franceschini ◽  
Bruno Ewerton da Silveira Cardillo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Liquid Medium ◽  
Common Bean ◽  
Bean Seed ◽  
Rhizobium Etli ◽  
Application Method ◽  
Application Rates ◽  
Reduced Costs ◽  
Liquid Inoculant

Abstract: The objective of this work was to evaluate the viability of liquid medium inoculation of Rhizobium etli in the planting furrow and to certify the efficiency of its strain UFLA 02-100 as a potential inoculant for common bean (Phaseolus vulgaris). The treatments consisted of three application rates of liquid inoculant applied in the planting furrows or in common bean seed, besides two controls without inoculation. The inoculant contained 109 cells of Rhizobium etli mL-1. Regardless of the application method, the yield obtained with the inoculation was equivalent to that of N from urea; however, the application in the furrows, at 0.6 L ha-1, is more advantageous due to the operational practicality and reduced costs.

Analysis of the Proteome of Common Bean (Phaseolus vulgaris L.) Roots after Inoculation with Rhizobium etli

2012 ◽  
Vol 19 (8) ◽  
pp. 880-889 ◽  
Author(s):  
Afshin Salavati ◽  
Alireza Taleei ◽  
Ali Akbar Shahnejat Bushehri ◽  
Setsuko Komatsu
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L

scholarly journals Rhizobium etli and Rhizobium gallicum Nodulate Common Bean (Phaseolus vulgaris) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

2003 ◽  
Vol 69 (2) ◽  
pp. 884-893 ◽  
Author(s):  
Claudia Silva ◽  
Pablo Vinuesa ◽  
Luis E. Eguiarte ◽  
Esperanza Martínez-Romero ◽  
Valeria Souza
Keyword(s):  
Genetic Structure ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Host Range ◽  
Polymorphism Analysis ◽  
Enzyme Electrophoresis ◽  
Rhizobium Etli ◽  
Genetic Barrier ◽  
Temporal Isolation ◽  
Rhizobium Gallicum

ABSTRACT The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs, glnII, nifH, and nodB sequences), along with host range experiments, placed the isolates examined in Rhizobium etli bv. phaseoli and Rhizobium gallicum bv. gallicum. Cluster analysis of multilocus enzyme electrophoresis and plasmid profile data separated the two species and identified numerically dominant clones within each of them. Population genetic analyses showed that there was high genetic differentiation between the two species and that there was low intrapopulation differentiation of the species over the 3 years. The results of linkage disequilibrium analyses are consistent with an epidemic genetic structure for both species, with frequent genetic exchange taking place within conspecific populations but not between the R. etli and R. gallicum populations. A subsample of isolates was selected and used for 16S ribosomal DNA PCR-restriction fragment length polymorphism analysis, nifH copy number determination, and host range experiments. Plasmid profiles and nifH hybridization patterns also revealed the occurrence of lateral plasmid transfer among distinct multilocus genotypes within species but not between species. Both species were recovered from nodules of the same plants, indicating that mechanisms other than host, spatial, or temporal isolation may account for the genetic barrier between the species. The biogeographic implications of finding an R. gallicum bv. gallicum population nodulating common bean in America are discussed.

scholarly journals Bio-protective effect of a root-nodulating Rhizobium etli strain in common bean (Phaseolus vulgaris) against Meloidogyne incognita and Radopholus similis in an in vitro autotrophic tripartite culture system

Nematology ◽  
2020 ◽  
pp. 1-9
Author(s):  
Lieselot Van der Veken ◽  
Pa Pa Win ◽  
Preeti Seeboruth ◽  
Ma. Teodora N. Cabasan ◽  
Rony Swennen ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Protective Effect ◽  
Common Bean ◽  
Meloidogyne Incognita ◽  
Culture System ◽  
Nematode Species ◽  
Rhizobial Strain ◽  
Radopholus Similis ◽  
Rhizobium Etli

Summary The bio-protective effect of a root-nodulating strain (CNPAF 512) of the nitrogen-fixing rhizobium, Rhizobium etli, against both a sedentary (Meloidogyne incognita) and a migratory (Radopholus similis) endoparasitic nematode in common bean (Phaseolus vulgaris) was examined using an in vitro autotrophic tripartite culture system. Two in vitro assays were carried out with each of the nematode species. Each assay consisted of two treatments: the plants were either inoculated with the rhizobial strain or remained non-inoculated (control plants). To examine the effect of either pre- or simultaneous inoculation of the rhizobial strain on the reproduction of M. incognita and R. similis, one assay was carried out in which the nematodes were inoculated 3 weeks after rhizobial inoculation while another assay was carried out in which the nematodes were inoculated simultaneously with the rihizobial strain. Both pre-inoculation and simultaneous inoculation with R. etli CNPAF 512 significantly suppressed the reproduction of both M. incognita and R. similis.

Genetic purity of common bean seed generations (Phaseolus vulgaris cv. 'INTA ROJO') as tested with microsatellite markers

2012 ◽  
Vol 40 (1) ◽  
pp. 73-85 ◽  
Author(s):  
O.R. Jiménez ◽  
H. Korpelainen ◽  
A. Rojas ◽  
P. Elomaa ◽  
J.P.T. Valkonen
Keyword(s):  
Phaseolus Vulgaris ◽  
Microsatellite Markers ◽  
Common Bean ◽  
Bean Seed ◽  
Genetic Purity

scholarly journals Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants

2008 ◽  
Vol 51 (1) ◽  
pp. 163-173 ◽  
Author(s):  
Cileide Maria Medeiros Coelho ◽  
Cláudia Mattos Bellato ◽  
Andréia Karime Marcelino Garcia ◽  
Victor Alexandre Vitorello ◽  
Ricardo Antunes Azevedo
Keyword(s):  
Abscisic Acid ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bean Seed ◽  
Phaseolus Vulgaris L ◽  
In Vitro Synthesis ◽  
Fruit Explants

The in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). Variation in phytate coincided with different concentrations of sucrose, myo-inositol, P and ABA for the duration tested. These compounds caused an accumulation of phytate and were more effective in the presence of myo-inositol and P. The accumulation of P varied less than phytate for the different treatments tested in vitro. In conclusion, P, sucrose, ABA, and myo-inositol caused an increase in the phytate of bean seed, showing that it could be possible to alter its content by culturing bean fruit explants in vitro.

scholarly journals Engineering the nifH Promoter Region and Abolishing Poly-β-Hydroxybutyrate Accumulation in Rhizobium etli Enhance Nitrogen Fixation in Symbiosis with Phaseolus vulgaris

2004 ◽  
Vol 70 (6) ◽  
pp. 3272-3281 ◽  
Author(s):  
Humberto Peralta ◽  
Yolanda Mora ◽  
Emmanuel Salazar ◽  
Sergio Encarnaci�n ◽  
Rafael Palacios ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Nitrogen Content ◽  
Seed Yield ◽  
Nitrogenase Activity ◽  
Genetic Material ◽  
Rhizobium Etli ◽  
Plant Weight ◽  
The Common

ABSTRACT Rhizobium etli, as well as some other rhizobia, presents nitrogenase reductase (nifH) gene reiterations. Several R. etli strains studied in this laboratory showed a unique organization and contained two complete nifHDK operons (copies a and b) and a truncated nifHD operon (copy c). Expression analysis of lacZ fusion demonstrated that copies a and b in strain CFN42 are transcribed at lower levels than copy c, although this copy has no discernible role during nitrogen fixation. To increase nitrogenase production, we constructed a chimeric nifHDK operon regulated by the strong nifHc promoter sequence and expressed it in symbiosis with the common bean plant (Phaseolus vulgaris), either cloned on a stably inherited plasmid or incorporated into the symbiotic plasmid (pSym). Compared with the wild-type strain, strains with the nitrogenase overexpression construction assayed in greenhouse experiments had, increased nitrogenase activity (58% on average), increased plant weight (32% on average), increased nitrogen content in plants (15% at 32 days postinoculation), and most importantly, higher seed yield (36% on average), higher nitrogen content (25%), and higher nitrogen yield (72% on average) in seeds. Additionally, expression of the chimeric nifHDK operon in a poly-β-hydroxybutyrate-negative R. etli strain produced an additive effect in enhancing symbiosis. To our knowledge, this is the first report of increased seed yield and nutritional content in the common bean obtained by using only the genetic material already present in Rhizobium.

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