scholarly journals Bio-protective effect of a root-nodulating Rhizobium etli strain in common bean (Phaseolus vulgaris) against Meloidogyne incognita and Radopholus similis in an in vitro autotrophic tripartite culture system

Nematology ◽  
2020 ◽  
pp. 1-9
Author(s):  
Lieselot Van der Veken ◽  
Pa Pa Win ◽  
Preeti Seeboruth ◽  
Ma. Teodora N. Cabasan ◽  
Rony Swennen ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Protective Effect ◽  
Common Bean ◽  
Meloidogyne Incognita ◽  
Culture System ◽  
Nematode Species ◽  
Rhizobial Strain ◽  
Radopholus Similis ◽  
Rhizobium Etli

Summary The bio-protective effect of a root-nodulating strain (CNPAF 512) of the nitrogen-fixing rhizobium, Rhizobium etli, against both a sedentary (Meloidogyne incognita) and a migratory (Radopholus similis) endoparasitic nematode in common bean (Phaseolus vulgaris) was examined using an in vitro autotrophic tripartite culture system. Two in vitro assays were carried out with each of the nematode species. Each assay consisted of two treatments: the plants were either inoculated with the rhizobial strain or remained non-inoculated (control plants). To examine the effect of either pre- or simultaneous inoculation of the rhizobial strain on the reproduction of M. incognita and R. similis, one assay was carried out in which the nematodes were inoculated 3 weeks after rhizobial inoculation while another assay was carried out in which the nematodes were inoculated simultaneously with the rihizobial strain. Both pre-inoculation and simultaneous inoculation with R. etli CNPAF 512 significantly suppressed the reproduction of both M. incognita and R. similis.

Effects of plant phenylpropanoid pathway products and selected terpenoids and alkaloids on the behaviour of the plant-parasitic nematodes Radopholus similis, Pratylenchus penetrans and Meloidogyne incognita

Nematology ◽  
2006 ◽  
Vol 8 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Nathalie Wuyts ◽  
Rony Swennen ◽  
Dirk De Waele
Keyword(s):  
Meloidogyne Incognita ◽  
Chemical Defence ◽  
Nematode Species ◽  
Biologically Active ◽  
Parasitic Nematodes ◽  
Pratylenchus Penetrans ◽  
Radopholus Similis ◽  
Irreversible Inhibitor ◽  
Wide Range

AbstractPlants produce a wide range of biologically active chemicals which have been extensively explored for nematode-antagonistic properties. Although phenylpropanoids are part of the chemical defence system of plants against pests and diseases, including parasitic nematodes, no comprehensive study exists which relates (levels of) phenylpropanoid compounds in roots to actual effects on nematode behaviour. Therefore, a broad spectrum evaluation was made of the effects of phenylpropanoids (simple phenolics and flavonoids) and selected monoterpenoids and alkaloids on the behaviour of the migratory endoparasites Radopholus similis and Pratylenchus penetrans and the sedentary endoparasite Meloidogyne incognita. In vitro bioassays assessed effects on chemotaxis, motility, viability and hatch. Compared with the other two nematode species, P. penetrans was remarkably insensitive to the test compounds. Only phloretin was (limited) hatch inhibitive. This property was shared by other chalcone-related compounds for R. similis. Repellents and motility inhibitors for R. similis and M. incognita were found among the simple phenolic compounds. Flavonols stood out as repellent compounds for both these nematode species, while they were, in their degraded form, also motility inhibitors for M. incognita. Dopamine was an attractant for R. similis, while ferulic acid was strongly motility inhibitive and toxic (LC50 of 120 μg ml−1) for this nematode species. Salicylic acid was a strong attractant for M. incognita. The compound was also nematicidal (LC50 of 46 μg ml−1) and an irreversible inhibitor of hatch.

Attraction and penetration of Musa by the burrowing nematode, Radopholus similis, in an autotrophic bipartite culture system

Nematology ◽  
2021 ◽  
pp. 1-14
Author(s):  
Suganthagunthalam Dhakshinamoorthy ◽  
Erwin J. Galon ◽  
Annemie Elsen ◽  
Dirk De Waele
Keyword(s):  
Culture System ◽  
Model System ◽  
Radopholus Similis ◽  
Susceptible Plant ◽  
Compartment System ◽  
Female Migration ◽  
Equal Distance ◽  
Burrowing Nematode ◽  
Nematode Chemotaxis

Summary Our objective was to discover the stages (pre- or post-infection) in which the resistance to burrowing nematode (Radopholus similis) occurs in two resistant banana (Musa spp.) cultivars. An autotrophic in vitro culture system was used to compare R. similis migration towards, and penetration into, the banana roots. A new two-compartment autotrophic in vitro model system was developed using agar-based medium to examine the migration of R. similis to either the susceptible ‘Grande Naine’ or the resistant ‘Yangambi km5’ (‘Ykm5’), when both the Musa genotypes were present at equal distance. The autotrophic in vitro model system was advantageous, because it supported continuous root growth due to the actively photosynthesising shoots growing in the open air, while the in vitro root conditions make it possible to observe and assess the nematode chemotaxis in the transparent medium. Significantly fewer nematodes migrated towards the resistant ‘Ykm5’ plants when compared to both the susceptible ‘Grande Naine’, and another resistant cultivar, ‘Saba’, at 1 h after infection. This signals a possibility of a lower concentration or different composition of nematode attractants in ‘Ykm5’ root exudates. No significant differences were observed in the percentage of R. similis that migrated towards the roots of the susceptible and resistant banana plants at 3, 4 and 6 h after inoculation. No significant differences were observed in the percentages of female penetration in the resistant and susceptible plant roots at 1 and 2 days after inoculation. The results of the two-compartment system confirmed that when a choice is given to migrate towards the resistant and susceptible genotypes, no differences were observed in the percentage of female migration towards both the genotypes.

Association of Gluconacetobacter diazotrophicus with roots of common bean (Phaseolus vulgaris) seedlings is promoted in vitro by UV light

2006 ◽  
Vol 84 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Alejandra Trujillo-López ◽  
Oscar Camargo-Zendejas ◽  
Rafael Salgado-Garciglia ◽  
Horacio Cano-Camacho ◽  
Víctor M. Baizabal-Aguirre ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Secondary Metabolite ◽  
Uv Light ◽  
Root Hairs ◽  
Bacterial Cells ◽  
Gluconacetobacter Diazotrophicus ◽  
Seedling Roots ◽  
Metabolite Accumulation

Gluconacetobacter diazotrophicus is a diazotrophic endophyte that is a potential biofertilizer. Little is known about the mechanisms of G. diazotrophicus interaction with its host plants. We tested the effect of UV light, as an inducer of secondary metabolite accumulation, on the association between common bean ( Phaseolus vulgaris L.) seedling roots and G. diazotrophicus. UV light irradiation of seedlings 4 h prior to bacterial inoculation increased the number of bacterial cells associated with the roots by 5.65-fold with respect to a nonirradiated control (p < 0.05). Gluconacetobacter diazotrophicus associates with root hairs and root border cells. Aggregation of bacterial cells was observed in root structures from UV-induced seedlings. Secondary metabolite accumulation was also observed in roots from UV-irradiated seedlings.

Analysis of the Proteome of Common Bean (Phaseolus vulgaris L.) Roots after Inoculation with Rhizobium etli

2012 ◽  
Vol 19 (8) ◽  
pp. 880-889 ◽  
Author(s):  
Afshin Salavati ◽  
Alireza Taleei ◽  
Ali Akbar Shahnejat Bushehri ◽  
Setsuko Komatsu
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L

Plant Parasitic Nematodes Associated with Plantain (Musa acuminata x M. balbisiana, AAB) in Puerto Rico

1969 ◽  
Vol 66 (1) ◽  
pp. 52-59
Author(s):  
D. Oramas ◽  
J. Román
Keyword(s):  
Puerto Rico ◽  
Meloidogyne Incognita ◽  
Nematode Species ◽  
Musa Acuminata ◽  
Parasitic Nematodes ◽  
Radopholus Similis ◽  
Plant Parasitic Nematodes ◽  
Rotylenchulus Reniformis ◽  
Phytoparasitic Nematodes ◽  
Plant Parasitic

A survey was conducted through the five argricultural regions of Puerto Rico to determine distribution and population density of the nematode species associated with plantain. Fourteen genera, represented by 19 species of phytoparasitic nematodes were found in association with the crop: Ditylenchus, Helicotylenchus, Hoplolaimus, longidorus, Meloidogyne, Paratylenchus, Pratylenchus, Pseudhalenchus, Quinisulcius, Radopholus, Rotylenchulus, Tylenchorhynchus, Tylenchus, and Xiphinema. Meloidogyne incognita, Helicotylenchus spp., Radopholus similis, and Rotylenchulus reniformis were the most widely distributed nematode species throughout the Island. On the other hand, M. incognita, P. coffeae, R. similis, Helicotylenchus spp. , and R. reniformis had higher population densities.

scholarly journals Viability of liquid medium-inoculation of Rhizobium etli in planting furrows with common bean

2018 ◽  
Vol 53 (3) ◽  
pp. 394-398
Author(s):  
Dâmiany Pádua Oliveira ◽  
Bruno Lima Soares ◽  
Fábio Aurélio Dias Martins ◽  
Leandro Alex Franceschini ◽  
Bruno Ewerton da Silveira Cardillo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Liquid Medium ◽  
Common Bean ◽  
Bean Seed ◽  
Rhizobium Etli ◽  
Application Method ◽  
Application Rates ◽  
Reduced Costs ◽  
Liquid Inoculant

Abstract: The objective of this work was to evaluate the viability of liquid medium inoculation of Rhizobium etli in the planting furrow and to certify the efficiency of its strain UFLA 02-100 as a potential inoculant for common bean (Phaseolus vulgaris). The treatments consisted of three application rates of liquid inoculant applied in the planting furrows or in common bean seed, besides two controls without inoculation. The inoculant contained 109 cells of Rhizobium etli mL-1. Regardless of the application method, the yield obtained with the inoculation was equivalent to that of N from urea; however, the application in the furrows, at 0.6 L ha-1, is more advantageous due to the operational practicality and reduced costs.

Reproduction and pathogenicity of Helicotylenchus multicinctus, Meloidogyne incognita and Pratylenchus coffeae, and their interaction with Radopholus similis on Musa

Nematology ◽  
2006 ◽  
Vol 8 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Thomas Moens ◽  
Mario Araya ◽  
Rony Swennen ◽  
Dirk De Waele
Keyword(s):  
Root Growth ◽  
Meloidogyne Incognita ◽  
Nematode Species ◽  
Root Weight ◽  
Radopholus Similis ◽  
Population Densities ◽  
Large Variability ◽  
Pratylenchus Coffeae ◽  
Concomitant Species ◽  
Pot Plants

Abstract The reproduction and pathogenicity of Helicotylenchus multicinctus, Meloidogyne incognita and Pratylenchus coffeae was studied on Musa AAA cv. Grande Naine pot plants and microplot plants. First, separate experiments using different inoculation numbers (between 218 and 4144) and different exposure times (between 8 and 22 weeks) were executed in pots for each nematode species. Helicotylenchus multicinctus suppressed root growth by 13%, comparing inoculated with uninoculated plants. In contrast, M. incognita stimulated root growth by 6.7%, while P. coffeae had no effect on root growth. The interaction between R. similis and each of these three nematode species was investigated in a concomitancy experiment in pots. Plants without concomitant species yielded the highest R. similis population densities but did not differ from plants previously inoculated with H. multicinctus and P. coffeae. Meloidogyne incognita was the only concomitant species that reduced R. similis population densities. Inoculation with R. similis affected the recovered numbers of P. coffeae albeit with large variability. In the microplot experiment, all four nematode species except H. multicinctus reduced the bunch weight of bananas, compared to uninoculated plants. Pratylenchus coffeae and R. similis increased root damage by 129 and 262%, respectively, while only the latter species suppressed root weight with 66%.

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